{"title":"Glycation of sunnhemp protein with dextran via dry heating: Thermal, micro-structural characterization, and amino acid profiling.","authors":"Rashmi Rawat, Charanjiv Singh Saini","doi":"10.1111/1750-3841.17490","DOIUrl":"https://doi.org/10.1111/1750-3841.17490","url":null,"abstract":"<p><p>This study aims to obtain sunnhemp protein isolate (SHPI) and dextran conjugates by dry heating method of Maillard conjugation. The effects of different incubation time (0, 1, 3, 5, 7, and 9 days) on the molecular flexibility, available lysine content, antioxidant properties, molecular structure, and thermal and micro-structural properties of conjugates were compared with SHPI (no conjugation) at 60°C and 79% relative humidity. The results indicated the formation of SHPI-dextran conjugates as confirmed by the change in molecular flexibility, lysine content, antioxidant activities, color, and water activity values. The molecular structure revealed the confirmation of covalent bonding between SHPI and dextran. Differential scanning calorimetry and thermo-gravimetric analysis results exhibited improvement in the thermal stability of SHPI when conjugated with dextran. The microstructural characterization showed that Maillard conjugation changed the surface structure of SHPI. The analysis of amino acid composition displayed that lysine, arginine, and phenylalanine were the dominant Maillard reaction sites of SHPI and dextran. Among all the conjugated samples, 5 days of incubation time was selected as an optimum condition for the development of SHPI-dextran conjugates on the basis of the aforementioned characterization. Overall, it was concluded that Maillard conjugation of sunnhemp protein with dextran via dry-heating technique could modify and improve its various attributes. PRACTICAL APPLICATION: The conjugation of plant proteins with polysaccharide through the Maillard reaction under dry heating conditions represents a natural and green technique for improving the techno-functional properties of proteins. The study has the potential to establish framework for the utilization of Sunnhemp protein isolate-dextran conjugates. This approach offers the potential for cost-effective production of emulsifiers and development of effective encapsulating matrices. The investigation expands on an underutilized plant protein source facilitating an alternative to animal-based proteins and contributing to the development of a sustainable circular bioeconomy.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Active cardboard box with palm wood waste powder and orange oil to prevent browning and quality loss in cabbage: Mode of action and potential for reuse.","authors":"Nirundorn Matan, Athakorn Promwee, Narumol Matan","doi":"10.1111/1750-3841.17493","DOIUrl":"https://doi.org/10.1111/1750-3841.17493","url":null,"abstract":"<p><p>Browning, caused by enzymatic activity and storage conditions, affects cabbage during cold storage and is crucial for customer acceptance. This study investigated the effect of cardboard packaging containing low concentrations of nano-orange oil (ONE) at 0.006% in palm wood waste powder for anti-browning and extending the shelf life of cabbage. The incorporation of ONE into palm wood powder (PWP) using different methods (soaking, vapor, vapor with ultrasonic device, and control) was examined before using the active PWP to develop cardboard cabbage packaging. The reuse of the active cabbage box packaging was also investigated for up to three reuses. The results showed that a greater anti-browning effect was achieved with cardboard packaging made from active PWP with orange oil vapor and an ultrasonic device compared to other adsorption methods, with significantly higher inhibition of the key browning enzyme activities of polyphenol oxidase (PPO) and peroxidase (POD). Additionally, antioxidant activity and bioactive compounds were improved, maintaining the bright green color of cabbage after 21 days of storage. The shelf life of cabbage stored in active cardboard was extended to at least 21 days compared to 5 days for the control. The active cabbage box with PWP and ONE vapor with an ultrasonic device showed potential for reuse at least two times. Limonene was found on the surface of stored cabbage and may be a key factor in antimicrobial activity, helping to control microbial growth on the cabbage surface within standard limits during long-term storage. This finding provides valuable guidance for reducing cabbage waste during transportation and storage from farm to market. PRACTICAL APPLICATION: This research offers new insights into active cardboard packaging made from palm wood powder with a low concentration of orange oil vapor to prevent browning and microbial growth in storage boxes. The optimal method for producing this packaging uses nano-orange oil vapor at 0.006% with an ultrasonic device, which could be feasible for large-scale production. The packaging effectively reduced PPO and POD enzyme activity, delaying browning and extending cabbage shelf life by at least threefold compared to the control, while maintaining color and freshness. This cost-effective method promotes the sustainable use of agricultural waste in the fresh vegetable industry, as it can be reused at least twice, benefiting farmers and reducing cabbage waste.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tamara Parada, Pablo Pardo, Javier Saurina, Sonia Sentellas
{"title":"Characterization of dark chocolates based on polyphenolic profiles and antioxidant activity.","authors":"Tamara Parada, Pablo Pardo, Javier Saurina, Sonia Sentellas","doi":"10.1111/1750-3841.17451","DOIUrl":"https://doi.org/10.1111/1750-3841.17451","url":null,"abstract":"<p><p>Dark chocolates were characterized according to geographical origin, cocoa variety, and cocoa content using the methylxanthine and polyphenolic composition and antioxidant activity as the data. The main study objective was to uncover sample patterns and identify possible markers of quality, variety, or origin to deal with authentication or fraud detection issues. In the study, a set of 26 dark chocolates from different varieties (e.g., Criollo, Forastero, and Trinitario) harvested in Africa, America, and Asia was analyzed. The optimized sample treatment consisted of defatting the chocolate (1 g of sample with 5 mL of cyclohexane for 15 min, three times) and then extracting the analytes by sonication with methanol/water 60:40 (v:v) for 15 min. The filtered extracts were analyzed by reversed-phase high-performance liquid chromatography with UV and spectrophotometric methods (Folin-Ciocalteu, ferric reducing antioxidant power, and aluminum methods) to determine individual phenolics and overall indexes of antioxidant and flavonoid content. Results from this chocolate set indicated that American samples are richer than African counterparts in alkaloids and phenolics (e.g., 1.7 vs. 1.1 mg g<sup>-1</sup> caffeine and 14.5 vs. 12.5 mg g<sup>-1</sup> total flavanols, respectively). Regarding cocoa varieties, Criollo cocoa was richer in bioactive compounds and antioxidant capacity (e.g., 16, 15, and 12 mg g<sup>-1</sup> total flavanols for Criollo, Forastero, and Trinitario, respectively). These results indicate that the analytes resulted in potential descriptors of varietal or geographical attributes.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Joshua Bernin, Preston Watanabe, Caleb E Wagner, Stephanie Smith, Girish M Ganjyal
{"title":"Mung bean protein enhances the expansion of corn starch during twin-screw extrusion.","authors":"Joshua Bernin, Preston Watanabe, Caleb E Wagner, Stephanie Smith, Girish M Ganjyal","doi":"10.1111/1750-3841.17375","DOIUrl":"https://doi.org/10.1111/1750-3841.17375","url":null,"abstract":"<p><p>This study examined the effects of the inclusion of mung bean protein (MBP) on the direct expansion characteristics of corn starch during twin-screw extrusion. Six blends of corn starch and MBP isolate (0%, 5%, 10%, 15%, 20%, and 25% w/w) were hydrated to three different moisture contents (MCs) (16%, 19%, and 21% w.b.). The blends were extruded using a twin-screw extruder at three screw speeds (SSs) (300, 400, and 500 rpm). The resulting extrudates were evaluated for their water solubility index, water absorption index, expansion ratio (ER), true density, unit density, and porosity. As the protein content increased, the porosity of the extrudates increased. The ER of all extrudates ranged from 2.90 to 5.46, with the largest ER observed at an SS of 400 rpm, an MC of 19%, and 25% MBP inclusion. The porosity of the extrudates ranged from 1.79% to 11.42%. SS and protein content had a significant impact (p < 0.05) on the porosity and ER of the extrudate. PRACTICAL APPLICATION: This work provides valuable information for the industry on utilizing mung bean protein in direct expanded corn starch-based extruded snacks. The information could be useful in the development of high-protein extruded snacks and breakfast cereals.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pitima Sinlapapanya, Suriya Palamae, Jirayu Buatong, Jaksuma Pongsetkul, Yu Fu, Bin Zhang, Soottawat Benjakul
{"title":"Ethanolic cashew leaf extract: Antifungal activity and its application for shelf-life extension of dried salted tilapia fillets.","authors":"Pitima Sinlapapanya, Suriya Palamae, Jirayu Buatong, Jaksuma Pongsetkul, Yu Fu, Bin Zhang, Soottawat Benjakul","doi":"10.1111/1750-3841.17504","DOIUrl":"https://doi.org/10.1111/1750-3841.17504","url":null,"abstract":"<p><p>Ethanolic cashew leaf extract (ECLE) is rich in phenolic compounds with diverse bioactivities and can serve as a safe natural preservative. This study evaluated the antifungal activity and application of ECLE for shelf-life extension of dried salted tilapia fillets. Several extraction methods, antifungal activity, and application of ECLE in dried salted tilapia fillets were investigated. Ultrasonication followed by the Soxhlet extraction resulted in the highest yield (26.78%), total phenolic content (TPC), and total flavonoid content (TFC) (p < 0.05). Conversely, the Soxhlet extraction method rendered lower yield (14.35%), TPC, and TFC (p < 0.05). NaCl at high concentrations decreased both TPC and TFC in all ECLE samples, demonstrating the decomposition of those compounds induced by NaCl. ECLE obtained via the Soxhlet extraction method exhibited lower minimum inhibitory concentration (MIC) and minimum fungicidal concentration values than those prepared using other extraction methods. Thus, the former showed higher efficacy in inhibiting fungal growth and reducing mycelium growth than others (p < 0.05), despite being less effective than potassium sorbate. At 4MIC, ECLE inhibited mycelium growth (56.83%-78.66%) and spore germination (87.5%-100%) after 72 h and 10-16 h of treatment, respectively. ECLE (4MIC) could inhibit the toxin production of fungi. For the challenge test, in which ECLE at 400 and 600 mg/kg was added to dried salted tilapia fillet inoculated with Aspergillus flavus, fungal growth was retarded over 9 days of storage at 25 ± 2°C (room temperature). Thus, ECEL could act as a natural food preservative to prevent fungal contamination. Toxin from fungi could be avoided, and the quality of dried salted fish was maintained. PRACTICAL APPLICATION: Cashew leaf extract rich in polyphenols can inhibit fungal proliferation, reduce mycelium expansion, prevent spore germination, and limit aflatoxin production. The extract can enhance the safety of dried salted fish, especially when contaminated with Aspergillus flavus in the humid atmosphere (80% relative humidity), particularly for small and medium enterprises. Nevertheless, this extract can also be applied in the fish processing industry, in which the synthetic antifungal agent could be replaced by the natural additive.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Improving sunflower oil stability with propolis: A study on antioxidative effects of Turkish propolis during accelerated oxidation.","authors":"Ayhan Baştürk, Berfin Yavaş","doi":"10.1111/1750-3841.17482","DOIUrl":"https://doi.org/10.1111/1750-3841.17482","url":null,"abstract":"<p><p>Propolis, a natural resinous substance collected by bees, is known for its potent antioxidant properties. This study investigates the antioxidant activities and total phenolic contents of propolis samples from 16 provinces of Türkiye and their effects on the oxidative stability of sunflower oil. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) inhibition was in the range of 28.1%-92.5% in thirteen propolis samples, whereas this rate was 24.5% in butylated hydroxytoluene (BHT). Although 2,2'-azino-bis(3-ethylbenzotiazolin-6-sulfonic acid) (ABTS) value was 224 µmol trolox/g in BHT, this value was in the range of 262-1370 µmol trolox/g in propolis samples, except for one. Propolis methanol extracts 13 applied to sunflower oil at a concentration of 1000 ppm were more efficient than BHT added at 200 ppm for inhibiting the production of peroxide value (PV). Similarly, most propolis extracts (1000 ppm) demonstrated antioxidant activity against the production of p-anisidine (p-AV) in sunflower oil under accelerated oxidation conditions. It was determined that Turkish propolis had strong antioxidant properties and delayed oxidation and may be utilized commercially in the food sector to delay the oxidation of fats and oils.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of different heat treatment methods on physicochemical characteristics and in vitro digestibility of sweet potato flour and its application in meal replacement flour.","authors":"Shunzhang Ma, Jingwen Zhang, Mingjun Peng, Ruiling Shen, Jilin Dong","doi":"10.1111/1750-3841.17481","DOIUrl":"https://doi.org/10.1111/1750-3841.17481","url":null,"abstract":"<p><p>This study examined the effects of various heat treatments on physicochemical properties, functionality, and starch digestibility of sweet potato flour. Heat treatments darkened the color but did not change the chemical structure. Solubility decreased and swelling power increased (17.3%-18.3%) with baking, frying, and microwaving, while extrusion and steaming had opposite effects. Rapidly digestible starch content increased (8.96%-41.91%) in all treatments except steaming, which reduced slowly digestible starch (61.81%-28.97%). Based on the analysis of these studies, using low-temperature baked and extruded sweet potato flour as the main raw material, combined with the concept of complete nutrition, supplemented by nutritious ingredients such as quinoa, oats, and whey protein, we have successfully developed two sweet potato meal replacement flours (SP-1 and SP-2) and found they had good brewing properties and showed shear-thinning behavior. This study provided theoretical basis for sweet potato deep processing and functional product development. PRACTICAL APPLICATION: This paper studied the effects of different heat treatments on sweet potato flour, and developed two meal replacement flour based on this, to provide a theoretical basis for the application of the sweet potato industry.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G Sánchez-Terrón, R Martínez, M J Freire, J Molina-Infante, M Estévez
{"title":"Gastrointestinal fate of proteins from commercial plant-based meat analogs: Silent passage through the stomach, oxidative stress in intestine, and gut dysbiosis in Wistar rats.","authors":"G Sánchez-Terrón, R Martínez, M J Freire, J Molina-Infante, M Estévez","doi":"10.1111/1750-3841.17458","DOIUrl":"https://doi.org/10.1111/1750-3841.17458","url":null,"abstract":"<p><p>Plant-based meat analogs (PBMAs) are common ultra-processed foods (UPFs) included in the vegan/vegetarian diets as presumed healthy alternatives to meat and meat products. However, such health claims need to be supported by scientific evidence. To gain further insight into this topic, two commercial UPFs typically sold as meat analogs, namely, seitan (S) and tofu (T), were included in a cereal-based chow and provided to Wistar rats for 10 weeks. A group of animals had, simultaneously, an isocaloric and isoprotein experimental diet formulated with cooked beef (B). In all cases, experimental chows (∼4 kcal/g feed) had their basal protein concentration increased from 14% to 30% using proteins from S, T, or B. Upon slaughter, in vivo protein digestibility was assessed, and the entire gastrointestinal tract (digests and tissues) was analyzed for markers of oxidative stress and untargeted metabolomics. Metagenomics was also applied to assess the variation of microbiota composition as affected by dietary protein. Diets based on PBMAs showed lower protein digestibility than those containing meat and promoted an intense luminal glycoxidative stress and an inflammatory intestinal response. The fermentation of undigested oxidized proteins from T in the colon of Wistar rats likely led to formation of mutagenic metabolites such as p-cresol. The presence of these compounds in the animal models raises concerns about the potential effects of full replacement of meat by certain PBMAs in the diet. Therefore, future research might target on translational human studies to shed light on these findings.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Nondestructive freshness prediction of large yellow croaker (Pseudosciaena crocea) using computer vision and machine learning techniques based on pupil color.","authors":"Xudong Wu, Qingxiang Zhang, Zhiqiang Wang, Zongmin Wang, Hongbo Yan, Lanlan Zhu, Jie Chang","doi":"10.1111/1750-3841.17412","DOIUrl":"https://doi.org/10.1111/1750-3841.17412","url":null,"abstract":"<p><p>Conventional methods for evaluating of fish freshness based on physiological and biochemical methods are often destructive, complicated, and costly. This study aimed to predict the freshness of large yellow croaker which was sampled every second day in 9 consecutive days at 4°C, using computer vision technology combined with pupil color parameters and different machine learning algorithms (back propagation neural network, BPNN; radial basis function neural network; support vector regression; and random forest regression, RFR). In the process of model building, the RFR model provided the most accurate prediction for the value of total volatile basic nitrogen (TVB-N), with the R-square of the test set ( <math> <semantics><msubsup><mi>R</mi> <mi>p</mi> <mn>2</mn></msubsup> <annotation>$R^{2}_p $</annotation></semantics> </math> ) of 0.993. The BPNN model exhibited the best fit for predicting the value of thiobarbituric acid (TBA), with <math> <semantics><msubsup><mi>R</mi> <mi>p</mi> <mn>2</mn></msubsup> <annotation>$R^{2}_p $</annotation></semantics> </math> of 0.959. Additionally, the RFR model was the most effective in forecasting total viable count (TVC), with <math> <semantics><msubsup><mi>R</mi> <mi>p</mi> <mn>2</mn></msubsup> <annotation>$R^{2}_p $</annotation></semantics> </math> of 0.935. After validation, the root mean square error values of the RFR model for predicting TVB-N value, TBA value, and TVC value were the lowest, which were 0.764, 0.067, and 0.219, respectively. It demonstrated the applicability and good predictive performance of the RFR model for predicting biochemical and microbiological indicators. These findings also demonstrated that monitoring the changes in pupil color could successfully predict the freshness of chilled fish. PRACTICAL APPLICATION: Application Scenario: Quality inspectors detect changes in the freshness of large yellow croaker in real time from the beginning of distribution to the selling site.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of in vitro simulated digestion and fermentation characteristics of the crude exopolysaccharide from Levilactobacillus brevis M-14.","authors":"Qi Wang, Gen Li, Wenjun Qin, Jin Cai, Nifei Wang","doi":"10.1111/1750-3841.17467","DOIUrl":"https://doi.org/10.1111/1750-3841.17467","url":null,"abstract":"<p><p>The present study employed an in vitro static digestion model to simulate the saliva-gastrointestinal digestion and fecal fermentation of exopolysaccharides (EPSs) extracted from Levilactobacillus brevis M-14, aiming to understand the dynamic changes in physicochemical properties, biological potential of EPS, as well as their impact on the human enteric microorganism. EPS was mainly composed of polysaccharides. The molecular weight (M<sub>w</sub>) showed that EPS comprised two parts with a high M<sub>w</sub> and a low M<sub>w</sub> fraction of 42.81 × 10<sup>4</sup> and 1.23 × 10<sup>4</sup> Da, respectively. EPSs mainly consisted of mannose, rhamnose, galacturonic acid, glucose, and galactose in a molar ratio of 0.42∶0.13∶0.21∶0.13∶0.11. In the simulated digestion process, EPS was relatively stable. Furthermore, simulated digestion increased the antioxidant and hypoglycemic capacities of EPS. During the fermentation stage, the total carbohydrate contents of EPS decreased by 20.19%. The M<sub>w</sub> of the two components of EPS decreased by 16.37% and 61.67%, respectively, and accompanied by the production of free monosaccharides. EPS had the potential to modulate the composition of gut microbiota, increasing the relative abundance of Enterococcus and Parabacteroides, while decreasing the relative abundance of Bacteroides. The pH decreased and total short-chain fatty acids contents increased, especially acetic acid and propionic acid. This research provided valuable insights into the potential application of EPS as a prebiotic agent.</p>","PeriodicalId":193,"journal":{"name":"Journal of Food Science","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}