An Electrochemical Aptamer-Based (E-AB) Sensor Using Ti3C2Tx(MXene) and cDNA-PAA for Detection of AFB1

IF 3.4 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Food Science Pub Date : 2025-09-23 DOI:10.1111/1750-3841.70571

Xiaoya Meng, Zhongyu Li, Zili Xia, Dongdong Chen, Zijing Yuan, Yang Han, Heng Zhang, Xia Sun, Jun Seop Lee, Usmanova Surayo Rakhmatzhanovna, Bobokalonov Jamshed Tolehmurodovich, Wenping Zhao

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Abstract

An electrochemical aptasensor based on Ti₃C₂T_x (MXene) and cDNA-PAA was established for sensitive detection of aflatoxin B1 (AFB1) in peanuts. The high specific surface area of Ti₃C₂T_x (MXene) contributed to improve electrochemical efficiency and enhancing sensor stability which were characterized by the Randles−Sevcik equation. The self-made polyacrylic acid (PAA) nanogel was added with aptamer complementary chain (cDNA) to form cDNA-PAA nano gel composite, which could realize the synergistic amplification of the difference between the electrochemical signals before and after the addition of AFB1. The sensing effect of cDNA-PAA was validated using DPV. Methylene blue (MB) was modified at the proximal 3′ termini of the aptamer (Apt-MB), meanwhile AuNPs was used to immobilize Apt-MB and improve the efficiency of electrochemical reactions. The competition between AFB1 and cDNA-PAA combined with Apt MB resulted in a significant change in the electrochemical signal current. In addition, the constructed electrochemical aptasensor had a lower detection limit of 1.0 × 10⁻³ ng/L for AFB1 under optimal detection conditions, and a detection range of 1 to 1000 ng/L. Moreover, peanuts with different concentrations of AFB1 were used as actual detection samples. Through the constructed sensor detection, the spiked recovery rates within the spiking range of 50 to 500 ng/mL were 97.76% to 101.84% (n = 3).

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基于Ti3C2Tx（MXene）和cDNA-PAA的电化学适配体（E-AB）传感器检测AFB1

建立了一种基于Ti3C2Tx （MXene）和cDNA-PAA的电化学感应传感器，用于花生中黄曲霉毒素B1 （AFB1）的灵敏检测。Ti3C2Tx （MXene）的高比表面积有助于提高电化学效率和增强传感器的稳定性，这是由Randles - Sevcik方程表征的。将自制的聚丙烯酸（PAA）纳米凝胶加入适体互补链（cDNA），形成dna -PAA纳米凝胶复合物，可实现对加入AFB1前后电化学信号差异的协同放大。用DPV验证了dna - paa的传感效果。在适配体（Apt-MB）近3′端修饰亚甲基蓝（MB），同时利用AuNPs固定Apt-MB，提高电化学反应效率。AFB1与dna - paa结合Apt MB的竞争导致了电化学信号电流的显著变化。此外，在最佳检测条件下，所构建的电化学感应传感器对AFB1的检出限为1.0 × 10−3 ng/L，检测范围为1 ~ 1000 ng/L。并以不同AFB1浓度的花生为实际检测样品。通过构建的传感器检测，在50 ~ 500 ng/mL加标范围内加标回收率为97.76% ~ 101.84% （n = 3）。

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来源期刊

Journal of Food Science 工程技术-食品科技

CiteScore

7.10

自引率

2.60%

发文量

412

审稿时长

3.1 months

期刊介绍： The goal of the Journal of Food Science is to offer scientists, researchers, and other food professionals the opportunity to share knowledge of scientific advancements in the myriad disciplines affecting their work, through a respected peer-reviewed publication. The Journal of Food Science serves as an international forum for vital research and developments in food science. The range of topics covered in the journal include: -Concise Reviews and Hypotheses in Food Science -New Horizons in Food Research -Integrated Food Science -Food Chemistry -Food Engineering, Materials Science, and Nanotechnology -Food Microbiology and Safety -Sensory and Consumer Sciences -Health, Nutrition, and Food -Toxicology and Chemical Food Safety The Journal of Food Science publishes peer-reviewed articles that cover all aspects of food science, including safety and nutrition. Reviews should be 15 to 50 typewritten pages (including tables, figures, and references), should provide in-depth coverage of a narrowly defined topic, and should embody careful evaluation (weaknesses, strengths, explanation of discrepancies in results among similar studies) of all pertinent studies, so that insightful interpretations and conclusions can be presented. Hypothesis papers are especially appropriate in pioneering areas of research or important areas that are afflicted by scientific controversy.