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Permodelan Koding pada Penelitian Kualitatif-Studi Kasus 模态案例研究
IF 3.7 3区 生物学
Nucleus Pub Date : 2022-07-02 DOI: 10.37010/nuc.v3i1.758
Hamzah Robbani
{"title":"Permodelan Koding pada Penelitian Kualitatif-Studi Kasus","authors":"Hamzah Robbani","doi":"10.37010/nuc.v3i1.758","DOIUrl":"https://doi.org/10.37010/nuc.v3i1.758","url":null,"abstract":"penelitian kualitatif pilihan untuk memberikan solusi dari keterbatasan metode kuantitatif dalam mengungkapkan persepsi suatu variabel oleh suatu responden. tujuan penelitian ini adalah untuk memberikan permodelan koding metode kualitatif dengan pendekatan studi kasus yang marak dilakukan peneliti muda untuk memecahkan suatu evaluasi bisnis proses maupun organisasi tempat mereka bekerja. metode penelitian ini menggunakan pendekatan fenomenologi dengan interview dan observasi sebagai teknik pengumpulan data. peneliti mengembangkan permodelan koding dengan tabel yang dapat digunakan peneliti untuk laporan penelitian kualitatif. koding menjadi isu yang menyulitkan peneliti pada metode kualitatif dengan berbagai tahapannya, namun pada penelitian ini dapat dipersingkat cukup dengan tiga tahapan saja","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2022-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79220514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mental Workload Analysis Using NASA-TLX Method on Customer Service Employees in Strategist Informa Social Media Division (PT Home Center Kawan Lama) 基于NASA-TLX方法的strategy Informa社交媒体部门客服员工心理负荷分析(PT Home Center Kawan Lama)
IF 3.7 3区 生物学
Nucleus Pub Date : 2022-06-14 DOI: 10.37010/nuc.v3i1.671
Ratih Permata Sari, Rini Setiowati, Anggi Oktaviani
{"title":"Mental Workload Analysis Using NASA-TLX Method on Customer Service Employees in Strategist Informa Social Media Division (PT Home Center Kawan Lama)","authors":"Ratih Permata Sari, Rini Setiowati, Anggi Oktaviani","doi":"10.37010/nuc.v3i1.671","DOIUrl":"https://doi.org/10.37010/nuc.v3i1.671","url":null,"abstract":"Kawan Lama Group has been established since 1955 and until now has several business fields engaged in the Retail, Industrial, Food and Beverage, Service, Property and E-commerce sectors. For more than half a century, Kawan Lama continues to grow and develop to employ more than 40,000 employees. Kawan Lama Group's vision is more than just a family business, we are a business for families. Our mission is to bring values to a better life through business development and sustainable growth. PT. Home Center Kawan Lama or Informa is a furniture company starting from home furniture, office furniture, to commercial space furniture. Informa provides more than 35,000 quality items that can meet the needs of customers to have their dream furniture. The NASA-TLX method was developed by Sandra. G Hart from NASA-Ames Research Center and Lowell E. Staveland from San Jose State University in 1981. This method performs multidimensional measurements by considering weights and grades. The level of the first factor relates to the work performed, while the other three factors relate to the subject. The average WWL (weighted workload) mental workload of customer service employees prior to the proposed work shift improvement, application of music therapy and motivation is the morning shift of 76.78, the afternoon shift of 77.97 and the night shift of 80. Average Results WWL (weighted workload) mental workload of employees in the morning, afternoon and evening shifts shows a high mental workload. The morning, afternoon and evening shifts have significant differences in physical needs, mental needs, time requirements, performance, stress levels and average WWL (weighted workload).","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2022-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83519111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Perancangan Sistem Informasi Akuntansi pada Bunda Laundry 洗衣女工的会计信息设计系统
IF 3.7 3区 生物学
Nucleus Pub Date : 2022-05-30 DOI: 10.37010/nuc.v3i1.716
Hapsari Widayani
{"title":"Perancangan Sistem Informasi Akuntansi pada Bunda Laundry","authors":"Hapsari Widayani","doi":"10.37010/nuc.v3i1.716","DOIUrl":"https://doi.org/10.37010/nuc.v3i1.716","url":null,"abstract":"Tujuan penelitian ini adalah membuat sistem terkomputerisasi pada Bunda Laundry  yang dapat membantu dalam proses administrasi dan pembuatan laporan keuangan, memudahkan pegawai untuk melihat ketersediaan stock bahan baku yang menunjang proses cuci setrika, membantu pegawai Bunda Laundry  untuk meningkatkan kualitas pelayanan administrasi terhadap pelanggan sehingga dapat meningkatkan penjualan, dan mengetahui proses perancangan sistem informasi akuntansi pada Bunda Laundry . Data yang digunakan adalah data primer. Metode pengumpulan data berupa observasi langsung ke objek penelitian yaitu Bunda Laundry. Metode pengembangan sistem meliputi tahap perencanaan sistem, tahap analisis sistem, tahap perancangan sistem, tahap implementasi sistem, serta tahap pengujian dan penggunaan sistem. Hasil penelitian yaitu proses pelayanan konsumen dan pencatatan pada Bunda Laundry  masih menggunakan sistem yang manual sehingga dibuatkan sistem yang terkomputerisasi. Prosedur pelayanan konsumen dimulai dari konsumen menaruh pakaiannya pada Bunda Laundry  lalu bagian administrasi memberikan kuitansi pembayaran dan setelah itu pakaian konsumen diberikan kepada bagian produksi untuk di proses. Bila sudah selesai konsumen dapat mengambilnya dan membayarnya. Bagian administrasi akan merekap pembayaran tersebut dan membuat laporan keuangan yang akan diperiksa oleh pemilik laundry . Perancangan sistem ini dilakukan dengan mendeskripsikan masalah dan menganalisisnya menggunakan analisis PIECES. Setelah mengetahui kendala yang terjadi pada Bunda Laundry, penulis melakukan rancangan sistem menggunakan alur data flow diagram (DFD) dan membuat database dengan entity relationship diagram (ERD). Setelah semua hal dilalui, proses selanjutnya adalah menggambarkan rancangannya secara sederhana.","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2022-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77267081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Estimation of the Open Unemployn Rate on Province Jambi Using the Double Exponential Smootthing 用双指数平滑法估计我省公开失业率
IF 3.7 3区 生物学
Nucleus Pub Date : 2022-05-26 DOI: 10.37010/nuc.v3i1.675
Anisa Rahmawati, Nayla Desviona, Tiara Puapita Sari
{"title":"Estimation of the Open Unemployn Rate on Province Jambi Using the Double Exponential Smootthing","authors":"Anisa Rahmawati, Nayla Desviona, Tiara Puapita Sari","doi":"10.37010/nuc.v3i1.675","DOIUrl":"https://doi.org/10.37010/nuc.v3i1.675","url":null,"abstract":"Unemployment is an employment problem that is often faced in every region, especially in Jambi Province.  The indicator used to determine the number of unemployed is the open unemployment rate (TPT).  High unemployment can be a major source of poverty that can lead to crime and hinder the process of economic development.  As an initial precaution for the government before the increase in unemployment, which is to estimate the future TPT in Jambi Province.  One of the methods used in estimating TPT in Jambi Province is the Double Exponential Smoothing Method from Holt.  The measure of the forecast error uses the Mean Absolute Percent Error (MAPE).  The aim is to obtain estimated TPT results in Jambi Province in the coming year, using Holt's Double Exponential Smoothing Method.  The estimation results obtained, for TPT data there is a MAPE value of 18.96% with parameters  = 0.9 and  = 0.2.  The estimation model obtained is = .  The estimation results show that the TPT rate will continue to increase next year, it can be taken into consideration for the government in determining future plans to reduce TPT in order to accelerate economic development in Jambi Province. ","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2022-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80006916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pengaruh Metode Pembelajaran Daring terhadap Prestasi Belajar Bahasa Inggris pada Siswa Kelas XI di SMKN 1 Bogor
IF 3.7 3区 生物学
Nucleus Pub Date : 2022-05-15 DOI: 10.37010/nuc.v3i1.899
Wahyu Utama
{"title":"Pengaruh Metode Pembelajaran Daring terhadap Prestasi Belajar Bahasa Inggris pada Siswa Kelas XI di SMKN 1 Bogor","authors":"Wahyu Utama","doi":"10.37010/nuc.v3i1.899","DOIUrl":"https://doi.org/10.37010/nuc.v3i1.899","url":null,"abstract":"Tujuan penelitian adalah untuk mengetahui seberapa besar pengaruh metode pembelajaran daring terhadap prestasi belajar Bahasa Inggris pada siswa kelas XI di SMKN 1 Bogor. Metode penelitian yang digunakan dalam penelitian ini adalah metode kuantitatif dengan pendekatan deskriptif untuk melihat seberapa besar pengaruh variabel bebas metode pembelajaran daring (X) terhadap variabel terikat prestasi belajar Bahasa Inggris (Y). Selanjutnya, teknik analisis yang digunakan yaitu teknik analisis koefisien korelasi, analisis koefisien determinasi, dan analisis regresi linear sederhana. Hasil penelitian ini menunjukkan bahwa nilai koefisien korelasi r sebesar -0,3178 yang berarti terdapat pengaruh antara metode pembelajaran daring terhadap prestasi belajar Bahasa Inggris pada siswa kelas XI di SMKN 1 Bogor. Koefisien determinasi diperoleh nilai sebesar 10% yang berarti variabel bebas metode pembelajaran daring (X) memberikan kontribusi sebesar 10% terhadap prestasi belajar Bahasa Inggris, sedangkan sisanya sebesar 90% dipengaruhi oleh variabel lain yang tidak diikutsertakan dalam penelitian ini. Persamaan linear sederhana dalam penelitian ini adalah Y = 96,889 - 0,137 X. Setelah itu, penelitian dilakukan dengan uji hipotesis menggunakan uji t secara parsial yang diperoleh nilai thitung = 2,743 dan ttabel pada df 67 (69-2) dengan taraf signifikan 0,05 secara dua arah sebesar 1,996 maka thitung > ttabel (2,743 > 1,996). Dengan adanya uji hipotesis ini, dapat disimpulkan bahwa terdapat pengaruh antara metode pembelajaran daring terhadap prestasi belajar Bahasa Inggris pada siswa kelas XI di SMKN 1 Bogor","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2022-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82184411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Peningkatan Loyalitas Pelanggan pada PT Home Center Indonesia Menggunakan Metode Algoritma C4.5 dan Metode CSI (Customer Satisfaction Index) 增加客户对PT Home Center印尼忠诚度使用算法C4.5和CSI方法(客户满意度指数)
IF 3.7 3区 生物学
Nucleus Pub Date : 2022-05-15 DOI: 10.37010/nuc.v3i1.636
Riri Cornellia, Anggi Oktaviani, Deny Wibisono
{"title":"Peningkatan Loyalitas Pelanggan pada PT Home Center Indonesia Menggunakan Metode Algoritma C4.5 dan Metode CSI (Customer Satisfaction Index)","authors":"Riri Cornellia, Anggi Oktaviani, Deny Wibisono","doi":"10.37010/nuc.v3i1.636","DOIUrl":"https://doi.org/10.37010/nuc.v3i1.636","url":null,"abstract":"PT Home Center Indonesia atau Informa merupakan sebuah perusahaan perabot dimulai dari perabot rumah, kantor, hingga perabot ruang komersial. Tujuan penelitian ini untuk mengetahui jumlah respons pelanggan yang puas dan tidak puas dalam pelayanan jasa yang diberikan perusahaan perabot dan mengetahui faktor yang mempengaruhi kepuasan dan loyalitas untuk menyesuaikan pelayanan jasa yang diharapkan pelanggan. Dengan memberikan peningkatan loyalitas terhadap pelanggan dan menggunakan metode yang tepat, untuk mengukur seberapa peningkatan kepuasan dan loyalitas konsumen menggunakan metode data mining, Algoritma C4.5 dan CSI (Customer Satisfaction Index) yang menggunakan atribut kepuasan dan loyalitas pelanggan dalam penelitian yang mencakup dari harga, fasilitas, pelayanan dan loyalitas. atribut pendukung seperti kepentingan dan kepuasan dari (bukti fisik, kehandalan, empati, daya tanggap, kepastian) Adanya metode ini diharapkan dapat mengatasi masalah yang ada pada perusahaan agar dapat memprediksi dan mengevaluasi untuk terciptanya kepuasan dan loyalitas pelanggan yang diharapkan konsumen sesuai dengan target perusahaan. Hasil penelitian pengambilan responden pada pelanggan hasil dari nilai entropy dan gain sebesar entropy 0.971 dan gain sebesar 0,821 dalam perhitungan Algoritma C4.5. hasil perhitungan tingkat kepuasan pelanggan nilai CSI di PT Home Center Indonesia mendapatkan skor sebesar 42,14% yang berada pada rentang angka nilai indeks kepuasan pelanggan 35 – 50%. Dengan hasil kepuasan pelanggan secara keseluruhan berada pada kriteria “kurang puas”.","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2022-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85419328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Workshop on RanBP2/Nup358 and acute necrotizing encephalopathy RanBP2/Nup358与急性坏死性脑病研讨会
IF 3.7 3区 生物学
Nucleus Pub Date : 2022-04-29 DOI: 10.1080/19491034.2022.2069071
Alexander F. Palazzo, Jomon Joseph, M. Lim, K. Thakur
{"title":"Workshop on RanBP2/Nup358 and acute necrotizing encephalopathy","authors":"Alexander F. Palazzo, Jomon Joseph, M. Lim, K. Thakur","doi":"10.1080/19491034.2022.2069071","DOIUrl":"https://doi.org/10.1080/19491034.2022.2069071","url":null,"abstract":"ABSTRACT Dominant missense mutations in RanBP2/Nup358 cause Acute Necrotizing Encephalopathy (ANE), a pediatric disease where seemingly healthy individuals develop a cytokine storm that is restricted to the central nervous system in response to viral infection. Untreated, this condition leads to seizures, coma, long-term neurological damage and a high rate of mortality. The exact mechanism by which RanBP2 mutations contribute to the development of ANE remains elusive. In November 2021, a number of clinicians and basic scientists presented their work on this disease and on the interactions between RanBP2/Nup358, viral infections, the innate immune response and other cellular processes.","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2022-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79753279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 7
Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis 盘基ostellum spastin参与了半封闭有丝分裂过程中的核膜动力学
IF 3.7 3区 生物学
Nucleus Pub Date : 2022-03-17 DOI: 10.1080/19491034.2022.2047289
Ulrike Schweigel, Petros Batsios, A. Müller-Taubenberger, R. Gräf, Marianne Grafe
{"title":"Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis","authors":"Ulrike Schweigel, Petros Batsios, A. Müller-Taubenberger, R. Gräf, Marianne Grafe","doi":"10.1080/19491034.2022.2047289","DOIUrl":"https://doi.org/10.1080/19491034.2022.2047289","url":null,"abstract":"ABSTRACT Dictyostelium amoebae perform a semi-closed mitosis, in which the nuclear envelope is fenestrated at the insertion sites of the mitotic centrosomes and around the central spindle during karyokinesis. During late telophase the centrosome relocates to the cytoplasmic side of the nucleus, the central spindle disassembles and the nuclear fenestrae become closed. Our data indicate that Dictyostelium spastin (DdSpastin) is a microtubule-binding and severing type I membrane protein that plays a role in this process. Its mitotic localization is in agreement with a requirement for the removal of microtubules that would hinder closure of the fenestrae. Furthermore, DdSpastin interacts with the HeH/ LEM-family protein Src1 in BioID analyses as well as the inner nuclear membrane protein Sun1, and shows subcellular co-localizations with Src1, Sun1, the ESCRT component CHMP7 and the IST1-like protein filactin, suggesting that the principal pathway of mitotic nuclear envelope remodeling is conserved between animals and Dictyostelium amoebae.","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2022-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73426864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
In situ nuclear matrix preparation in Drosophila melanogaster embryos/tissues and its use in studying the components of nuclear architecture 黑腹果蝇胚胎/组织原位核基质制备及其在核结构组分研究中的应用
IF 3.7 3区 生物学
Nucleus Pub Date : 2022-03-03 DOI: 10.1080/19491034.2022.2043608
R. Pathak, Ashish Bihani, Rahul Sureka, Parul Varma, R. Mishra
{"title":"In situ nuclear matrix preparation in Drosophila melanogaster embryos/tissues and its use in studying the components of nuclear architecture","authors":"R. Pathak, Ashish Bihani, Rahul Sureka, Parul Varma, R. Mishra","doi":"10.1080/19491034.2022.2043608","DOIUrl":"https://doi.org/10.1080/19491034.2022.2043608","url":null,"abstract":"ABSTRACT The study of nuclear matrix (NuMat) over the last 40 years has been limited to either isolated nuclei from tissues or cells grown in culture. Here, we provide a protocol for NuMat preparation in intact Drosophila melanogaster embryos and its use in dissecting the components of nuclear architecture. The protocol does not require isolation of nuclei and therefore maintains the three-dimensional milieu of an intact embryo, which is biologically more relevant compared to cells in culture. One of the advantages of this protocol is that only a small number of embryos are required. The protocol has been extended to larval tissues like salivary glands with little modification. Taken together, it becomes possible to carry out such studies in parallel to genetic experiments using mutant/transgenic flies. This protocol, therefore, opens the powerful field of fly genetics to cell biology in the study of nuclear architecture. Summary: Nuclear Matrix is a biochemically defined entity and a basic component of the nuclear architecture. Here we present a protocol to isolate and visualize Nuclear Matrix in situ in the Drosophila melanogaster and its potential applications.","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2022-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86152385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Mammalian nuclear speckles exhibit stable association with chromatin: a biochemical study 哺乳动物核斑与染色质稳定相关:一项生化研究
IF 3.7 3区 生物学
Nucleus Pub Date : 2022-02-27 DOI: 10.1080/19491034.2021.2024948
K. Raina, B. Rao
{"title":"Mammalian nuclear speckles exhibit stable association with chromatin: a biochemical study","authors":"K. Raina, B. Rao","doi":"10.1080/19491034.2021.2024948","DOIUrl":"https://doi.org/10.1080/19491034.2021.2024948","url":null,"abstract":"ABSTRACT Nuclear Speckles (NS) are phase-separated condensates of protein and RNA whose components dynamically coordinate RNA transcription, splicing, transport and DNA repair. NS, probed largely by imaging studies, remained historically well known as Interchromatin Granule Clusters, and biochemical properties, especially their association with Chromatin have been largely unexplored. In this study, we tested whether NS exhibit any stable association with chromatin and show that limited DNAse-1 nicking of chromatin leads to the collapse of NS into isotropic distribution or aggregates of constituent proteins without affecting other nuclear structures. Further biochemical probing revealed that NS proteins were tightly associated with chromatin, extractable only by high-salt treatment just like histone proteins. NS were also co-released with solubilised mono-dinucleosomal chromatin fraction following the MNase digestion of chromatin. We propose a model that NS-chromatin constitutes a “putative stable association” whose coupling might be subject to the combined regulation from both chromatin and NS changes. Abbreviations: NS: Nuclear speckles; DSB: double strand breaks; PTM: posttranslational modifications; DDR: DNA damage repair; RBP-RNA binding proteins; TAD: topologically associated domains; LCR: low complexity regions; IDR: intrinsically disordered regions.","PeriodicalId":19392,"journal":{"name":"Nucleus","volume":null,"pages":null},"PeriodicalIF":3.7,"publicationDate":"2022-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91053833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
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