Nouvelle revue francaise d'hematologie最新文献_第5页

[Eprex and daily autologous transfusion]. [依普雷克斯与每日自体输血]。

Nouvelle revue francaise d'hematologie Pub Date : 1995-01-01

M Chassaigne, I Desbois, C Decilap

引用次数: 0

Study of the vesicles released during conservation of red cells. 红细胞保存过程中释放的囊泡的研究。

Nouvelle revue francaise d'hematologie Pub Date : 1995-01-01

N Ghailani, C Guillemin, C Vigneron

引用次数: 0

Cerebral venous sinus thrombosis associated with refractory anaemia with excess of blasts. 脑静脉窦血栓形成与细胞过多的难治性贫血相关。

Nouvelle revue francaise d'hematologie Pub Date : 1995-01-01

A Vayá, L Larrea, F Gomis, Y Mira, J Aznar

引用次数: 0

[A new indication for Sandimmum (cyclosporine A). Press release from Sandoz Laboratories]. [Sandimmum(环孢素A)的新适应症。山德士实验室新闻稿]。

Nouvelle revue francaise d'hematologie Pub Date : 1995-01-01

引用次数: 0

Presence of functional P2T and P2U purinoceptors on the human megakaryoblastic cell line, Meg-01 characterization by functional and binding studies. 功能P2T和P2U嘌呤受体在人巨核母细胞系上的存在，通过功能和结合研究鉴定Meg-01。

Nouvelle revue francaise d'hematologie Pub Date : 1995-01-01

B Hechler, J P Cazenave, D Hanau, C Gachet

{"title":"Presence of functional P2T and P2U purinoceptors on the human megakaryoblastic cell line, Meg-01 characterization by functional and binding studies.","authors":"B Hechler, J P Cazenave, D Hanau, C Gachet","doi":"","DOIUrl":"","url":null,"abstract":"The platelet receptor for ADP has been classified as a P2 purinoceptor of the P2T type where ADP is the natural agonist and ATP a competitive antagonist. Since the P2T receptor seems to be specific to platelets, we investigated the possibility that the human megakaryoblastic cell line Meg-01 might express the platelet ADP receptor, using functional studies and the ADP analogue [33P]2MeSADP as a specific P2T radioligand. ADP and 2MeSADP were able to induce shape change and pseudopod formation in Meg-01 cells. [33P]2MeSADP binding to membrane preparations was saturable and specific, binding sites being of high affinity (Kd = 13 +/- 3 nM) and present at a concentration of 13 +/- 7 fmoles/mu g protein. In contrast to UTP and GTP, ADP and ATP were able to displace the specific [33P]2MeSADP binding. ADP, 2MeSADP, UTP and ATP induced a dose dependent increase in intracellular calcium concentration. Desensitization experiments demonstrated that UTP, and ADP share a common P2U purinoceptor and that Meg-01 cells also express a P2T purinoceptor for which ADP and 2MeSADP are agonists and ATP a competitive antagonist. It was concluded that the human megakaryoblastic cell line Meg-01 expresses two distinct types of functional P2 receptor; a P2U and a P2T purinoceptor. This cell line could therefore provide the necessary material to clone the as yet unidentified platelet P2T purinoceptor.","PeriodicalId":19366,"journal":{"name":"Nouvelle revue francaise d'hematologie","volume":"37 4","pages":"231-40"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19868440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pregnancy occurring during complete cytogenetic response after interferon therapy in chronic myeloid leukaemia. 慢性髓性白血病干扰素治疗后细胞遗传学完全应答期间发生妊娠。

Nouvelle revue francaise d'hematologie Pub Date : 1995-01-01

L Longree, P Pautier, F Guilhot, P Brice

引用次数: 0

Imputability of heparin in heparin induced thrombocytopenia: a 10 year experience of the Centre Regional de Pharmacovigilance of Reims-Champagne Ardenne. 肝素诱发的血小板减少症中肝素的归因性:兰斯-香槟-阿登地区药物警戒中心的10年经验。

Nouvelle revue francaise d'hematologie Pub Date : 1995-01-01

P Nguyen, M L Germain, L Sume, H Choisy

引用次数: 0

Platelet procoagulant activity is independent of aggregation. 血小板促凝活性与聚集无关。

Nouvelle revue francaise d'hematologie Pub Date : 1995-01-01

F Rendu, P Lavie, S Saleun, D Lasne

{"title":"Platelet procoagulant activity is independent of aggregation.","authors":"F Rendu, P Lavie, S Saleun, D Lasne","doi":"","DOIUrl":"","url":null,"abstract":"Platelet prothrombinase activity and aggregation were studied in parallel in the same platelet suspensions with the aim of determining the relationship between these two responses. Stimulation of platelets with thrombin (0.12 U/ml) plus collagen (20 mu g/ml) led to maximum thrombin generation when the aggregation intensity was still only 30%. Since the rate of thrombin formation then remained constant as aggregation intensity was still only 30%. Since the rate of thrombin formation then remained constant as aggregation increased up to its maximum intensity, the aggregation processes did not appear to result in partial masking of any procoagulant surface. This was confirmed by the fact that thrombin generation was the same on aggregated platelets as on activated platelets prevented from aggregating by addition of RGDS. Finally, maximum 5-hydroxy-tryptamine release could be obtained in the absence of thrombin generation. Thus platelet procoagulant activity did not appear to be related to aggregation or secretion and thrombin formation on the surface of activated platelets was in fact two to three times more important in the absence of stirring than under the conditions of continuous stirring required for aggregation. In conclusion, these results suggest procoagulant activity and aggregation to be two independent platelet responses which occur in different physiological situations.","PeriodicalId":19366,"journal":{"name":"Nouvelle revue francaise d'hematologie","volume":"37 6","pages":"327-31"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19871435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro expansion of CD34+ cells from peripheral blood of myeloma and lymphoma patients. 骨髓瘤和淋巴瘤患者外周血CD34+细胞的体外扩增。

Nouvelle revue francaise d'hematologie Pub Date : 1995-01-01

B Mahe, A Menard, F Accard, D Pineau, N Robillard, S Hermouet

{"title":"In vitro expansion of CD34+ cells from peripheral blood of myeloma and lymphoma patients.","authors":"B Mahe, A Menard, F Accard, D Pineau, N Robillard, S Hermouet","doi":"","DOIUrl":"","url":null,"abstract":"We studied the feasibility of in vitro expansion of CD34+ cells from patients with multiple myeloma (MM) or follicular non Hodgkin lymphoma (NHL). CD34+ cells were selected from peripheral blood (PB) using avidinbiotin immunoadsorption columns: purified CD34+ cells from three MM and five NHL patients were expanded. First, CD34+ cells (2 MM, 4 NHL) were grown for 14 days in 5 ml of IMDM plus 12.5% horse serum (HS), 12.5% fetal calf serum (FCS) and a commonly used combination of cytokines: IL1alpha, IL3, IL6, SCF, GM-CSF, G-CSF (10 ng/ml each) and EP (4 UI/ml). In these conditions, at day 14, average increase in CD34+, CFU-GM and total cell numbers were, respectively: x 6.0 x 23 and x 2,113 fold with 20 to 35% of granulocytic cells. In terms of CD34+ cell, CFU-GM and total cell outputs, MM cultures were comparable to NHL cultures, but MM cultures seemed to produce less granulocytic cells than NHL cultures. Next, in vitro expansion of PB CD34+ cells was tested in culture media suitable for clinical use. Two cultures (1 MM, 1 NHL) were carried out for 14 days in 20 ml of X-Vivo 10 medium, 2% human serum, IL1alpha, IL3, IL6, SCF, GM-CSF, G-CSF (6 ng/ml each) and EP (2 UI/ml). Increase in CD34+, CFU-GM and total cell numbers in these conditions were, respectively: x 5.7 and x 19.7, x 11.9 and x 40.9, x 424 and x 408 fold, with at least 75% of granulocytic cells in both cultures. We conclude that, although further improvements are necessary, in vitro expansion of PB CD34+ cells can presumably be carried out successfully for MM patients as well as for NHL patients, including in conditions suitable for clinical use.","PeriodicalId":19366,"journal":{"name":"Nouvelle revue francaise d'hematologie","volume":"37 6","pages":"335-41"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19873340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Peripheral blood CD34+ cells: method of purification and ex vivo expansion. 外周血CD34+细胞:纯化和体外扩增方法。

Nouvelle revue francaise d'hematologie Pub Date : 1995-01-01

A Bohbot, O Feugeas, J M Cuillerot, A Grosse, I Rusciani, A Faradji, F Oberling

{"title":"Peripheral blood CD34+ cells: method of purification and ex vivo expansion.","authors":"A Bohbot, O Feugeas, J M Cuillerot, A Grosse, I Rusciani, A Faradji, F Oberling","doi":"","DOIUrl":"","url":null,"abstract":"Peripheral blood stem cells (PBSC) are used increasingly for autotransplantation in the treatment of acute leukemia, lymphoma, multiple myeloma, solid tumors such as ovarian and breast carcinoma. They are collected by leukaphereses during rapid hematopoietic recovery, following cytotoxic chemotherapy with or without administration of hematopoietic growth factors. We studied the clonogenic and cytokine-mediated expansion potential of CD34+ cells from mobilized PBSC. Low density mononuclear cells were processed using the CEPRATE LC CD34 KIT (CellPro). CD34+ purified cells, were cultured in suspension with 6 combined hematopoietic growth factors (IL1beta, IL3, IL6 at 100 U/ml and G-CSF, GM-CSF and stem cell factor at 10 ng/ml of each) for up to four weeks. Every week, cells were counted and CFU-GM assay was performed in a methylcellulose based medium. We have analysed the percentage of cells bearing CD34, CD33, CD38, HLA-DR, CD45RA, CD45RO antigens. Our results showed, that CD34+ cells were obtained with a purity of 92 +/- 2.3% and a yield of 71 +/- 10.7%. The majority co-expressed CD33 (57.76 +/- 34.16%) and CD38 (62.2 +/- 34%) antigens. These culture conditions, are necessary to obtain a fold increase of nucleated cells (377 fold at week 4), of CFU-GM progenitors (41.2 fold at week 3) and of CD34+ cell absolute number (10 fold at week 1) with an important differentiation of progenitors in particular myeloid progenitors.","PeriodicalId":19366,"journal":{"name":"Nouvelle revue francaise d'hematologie","volume":"37 6","pages":"359-65"},"PeriodicalIF":0.0,"publicationDate":"1995-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19873343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0