Alondra SALCIDO-MARTÍNEZ, Esteban Sánchez, Sandra PÉREZ-ÁLVAREZ, C. A. Ramírez-Estrada
{"title":"Agronomic biofortification with magnesium nanofertilizer and its effect on the nutritional quality of beans","authors":"Alondra SALCIDO-MARTÍNEZ, Esteban Sánchez, Sandra PÉREZ-ÁLVAREZ, C. A. Ramírez-Estrada","doi":"10.15835/nbha51413246","DOIUrl":"https://doi.org/10.15835/nbha51413246","url":null,"abstract":"Crop quality has been compromised due to nutrient deficiencies. The macronutrient magnesium (Mg) is essential; however, it has not been considered in agronomic fertilization programs, affecting human health. The objective of the study was to increase the Mg content in the fruits of green beans cv. ‘Strike’ by applying Mg-nanofertilizer, as well as evaluating its effect on growth, performance and nutritional quality, versus magnesium sulfate (MgSO4). The experiment was carried out under shade mesh conditions in Delicias, Chihuahua, Mexico during the period August-October 2022. A completely randomized experimental design was used, with two Mg sources: Mg nanofertilizer (NanoMg) and MgSO4 at doses of 50, 100 and 200 ppm and a control without application, forming seven treatments with six repetitions each. The results indicate that the maximum yield was provided by NanoMg and MgSO4 at 200 ppm, with values greater than 300%. The Mg distribution pattern for the NanoMg treatments presented the following concentration order: root>leaf>stem>fruit; while, for the MgSO4 treatments it was: leaf>root>stem>fruit. The most efficient treatment in increasing the Mg content in the fruit was NanoMg at 200 ppm, which achieved a biofortification of more than 120% with respect to the control. Therefore, when consuming 100 g of green beans cv. ‘Strike’ biofortified by NanoMg, the recommended daily needs of the human being could be satisfied. Finally, it is concluded that nanofertilizers are the best option for a biofortification program since they offer a sustainable alternative by increasing productivity and quality in green bean fruits.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"34 3","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139266832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Agro-mophological characterization, adaptational behaviour and flower abnormalities of Lilium L. cultivars in addition to a flower with stochastic flower formula","authors":"Aysun Çavuşoğlu","doi":"10.15835/nbha51412949","DOIUrl":"https://doi.org/10.15835/nbha51412949","url":null,"abstract":"This experiment was undertaken to determine the adaptation of Lilium L. cultivars to North-Western part of Türkiye and to define morphologic plant growth characteristics at aboveground part, including flower life and flower abnormalities. The bulbs of the three Lilium L. cultivars, ‘Profundo’, ‘Valverde’, and ‘Vonq’, were sown to perlite in pots in June, 2021, and evaluated for emergence and flowering rates, stem and plant height, stem and flower diameter, leaf and flower number, flower abnormality rates, flower fragrance degree and flower life in 2022, after one year adaptation without removing the previous sown bulbs. As the results of the cultivars differences these were determined that, at the adaptive year; the emergence rates varied between 60.42-89.58%, flowering rates changed between 43.75-87.50%, stem heights changed between 40.63-48.25 cm, plant heights changed between 52.50-59.75 cm and these data showed statistically importance. In addition to the data in the adaptive year; stem diameters, leaf numbers, flower numbers flower fragrance degree and flower longevities also showed statistical importance between cultivars. The flower abnormality rates were found between 8.3-25% in cultivars although no statistical results. Interestingly a striking, stochastic case also was observed from a flower with 8 tepals, 8 stamen and 4 lobed stigma at ‘Valverde’ contrary to well-known flower formula. It has been concluded that ‘Profundo’ has more remarkable result in terms of longevity of flower compared to the other two varieties while ‘Valverde’ and ‘Vonq’ shared the same group with some precedence in most of the agro-morphologic characteristics e.g. plant height, stem height and stem diameter. Lilium L. ‘Vonq’ showed that the highest adaptive capacity among the cultivars with the highest emergence rate (89.58) in all sowed bulbs and flowering rate (87.50) in all emerged bulbs under the study conditions in the open-air.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"13 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139272156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Saensouk, Wipa Yaowachai, Theeraphan Chumroenphat, Sukanya Nonthalee, P. Saensouk
{"title":"In vitro regeneration, transplantation and phytochemical profiles of Kaempferia angustifolia Roscoe","authors":"S. Saensouk, Wipa Yaowachai, Theeraphan Chumroenphat, Sukanya Nonthalee, P. Saensouk","doi":"10.15835/nbha51413190","DOIUrl":"https://doi.org/10.15835/nbha51413190","url":null,"abstract":"Kaempferia angustifolia Roscoe is a medicinal plant in the family Zingiberaceae, with wild occurrence decreasing as a result of both natural and human threats. Tissue culture techniques provide an alternative method of propagation for mass production. Microshoots of K. angustifolia (1 cm in length) were cultured on MS medium supplemented with various concentrations of cytokinins (BA and Kinetin) and auxins (NAA and IAA) for 8 weeks. Phytochemical profiles were evaluated by total phenolic contents (TPC), total flavonoid contents (TFC), antioxidant activity (2,2-Diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing ⁄ antioxidant power (FRAP) assay) and Gas chromatography–mass spectrometry (GC–MS) analysis. Maximum number of shoots was observed in medium supplemented with 2 mg/l BA plus 0.5 mg/l NAA (6.33 shoots/explant) and optimal rooting was induced in MS medium with 2 mg/l Kinetin plus 1 mg/l NAA (35.70 roots/explant). Highest numbers of shoots and roots were obtained when cultured on liquid MS medium supplemented with 2 mg/l BA, 2 mg/l Kinetin and 1 mg/l NAA (8.73 shoots/explant and 29.67 roots/explant, respectively). Regenerated plantlets of K. angustifolia were transferred to pots containing different types of plant materials under natural conditions for 8 weeks. Optimal survival rate was 100% when transplanting K. angustifolia to soil, sand, soil and sand (1:1) or soil and small pieces of rock (1:1) ratio. The methanol extract of leaves K. angustifolia from natural plants and in vitro derives plants showed a significantly higher amount of TFC and antioxidant activity. GC-MS analysis identified 52 phytochemical compounds in leaves of K. angustifolia. This study may be helpful to increase the value of commercial production of K. angustifolia, pharmaceutical and medicinal purpose.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"126 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139272956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hexane extract of Telosma cordata enhances neurite outgrowth via the epigenetically regulated genes expression in neuronal cells","authors":"Nguyet Thi DOAN, Hung Minh NGUYEN, Tuyet Loan Thi BUI, Huyen Trang Thi NGUYEN, Duong Hoang Nguyen, Truyen van Nguyen","doi":"10.15835/nbha51413204","DOIUrl":"https://doi.org/10.15835/nbha51413204","url":null,"abstract":"Telosma cordata has long been consumed as foods and herbal medicine. The aim of this research is to investigate the potential biological activities of Telosma cordata flower (TCF) extracts and their molecular mechanisms in the treatment of neurological diseases, especially dementia. In general, the plant samples were successfully identified through DNA barcoding regions, utilizing matK, trnH-psbA, and rbcL markers. The ethanol extract and its fraction exhibited significantly high acetylcholinesterase inhibitory activity. Moreover, hexane extract of T. cordata displayed the most potent neurotrophic activity in a preliminary cell-based screening based on C6 cells neurite outgrowth. Additionally, hexane extract of T. cordata demonstrated the highest antioxidant activity. Finally, the hexane extract of T. cordata upregulated the expression of BDNF, NGF and acetyl H3 in C6 cells, affecting both mRNA and protein levels. These findings indicate that Telosma cordata could be a strong candidate for developing pharmacological drugs to treat dementia and neurodegenerative diseases.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"34 2","pages":""},"PeriodicalIF":1.8,"publicationDate":"2023-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139273652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Introduction pages, NBHA-CN 51(3), 2023","authors":"Radu E. SESTRAS","doi":"10.15835/nbha51313432","DOIUrl":"https://doi.org/10.15835/nbha51313432","url":null,"abstract":"The papers published in Notulae Botanicae Horti Agrobotanici Cluj-Napoca, Issue 3, Volume 51, 2023 represent new exciting research in different topics of life science, respectively in plant science, horticulture, agronomy, crop science, and forestry. Among the interesting articles we invite you to find news about: Comparison of the potential of extracts from the flower, fruit pulp, and seed of Cassia fistula L. on MCF-7 breast cancer cell growth and cell migration; Evaluation of anticancer activity of Plumbago indica root extract on cervical HeLa cancer cells; Metabolome and transcriptome analyses reveal the colouring mechanism of red honeysuckle (Lonicera japonica Thunb.); Ethylene modulates root growth and mineral nutrients levels in trifoliate orange through the auxin-signaling pathway; Green nanotechnology for plant bacterial diseases management in cereal crops: a review on metal-based nanoparticles; Physiological responses of coffee (Coffea arabica L.) plants to biochar application under water deficit conditions; Gene mining and functional analysis related to maize (Zea mays L.) seed size; Phytochemical properties, antioxidant potential and fatty acids profiling of three dragon fruit species grown under sub-tropical climate; Chemical composition and anti-inflammatory effect of Phellodendron amurense Rupr. stem bark extract; Ecotype effects on photosynthesis performance using A/PFFD among Pinus nigra Arn.; Assessment of herbaceous vegetation classification using orthophotos produced from the image acquired with unmanned aerial systems.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135246917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mehvish RAMZAN, Wang LIHONG, Huma TARIQ, Syed F.A. GILLANI, Saima SHAFIQUE, Adnan RASHEED, Mahmoud F. SELEIMAN
{"title":"Assessment of genetic divergence in wheat lines (Triticum aestivum L.) involving biochemical and protein markers in rainfed conditions","authors":"Mehvish RAMZAN, Wang LIHONG, Huma TARIQ, Syed F.A. GILLANI, Saima SHAFIQUE, Adnan RASHEED, Mahmoud F. SELEIMAN","doi":"10.15835/nbha51313313","DOIUrl":"https://doi.org/10.15835/nbha51313313","url":null,"abstract":"Wheat (Triticum aestivum L.) is the important and strategic cereal crop for the majority of world’s populations. It is significant staple food of about two billion people. In next few years, world demand for wheat is expected to be 40 percent higher than that of its level today. Keeping in view the importance of the crop research work was conducted in the laboratory of Plant Breeding and Molecular Genetics, University of Poonch Rawalakot, AJK, Pakistan. The aim of the research was to find out the genetic diversity of different wheat lines. In the experiment, 50 different lines of wheat species (Triticum aestivum L.) was used to detect genetic diversity by utilizing Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and biochemical analysis. On the basis of biochemical analysis lines 3111 and 3123 was diverse among 50 lines for antioxidant activity by using DPPH radical and 3135 and 3139 for phenolic contents and for flavonoid 3148 and 3107 was found more promising. Molecular characterization by SDS PAGE showed diversity in three wheat lines 3136, 3138 and 3110. These wheat lines could be our potential lines for future wheat improvement program as they were also promising regarding to the high yields.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135426018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Muhammad T. ASLAM, Imran KHAN, Muhammad U. CHATTHA, Rizwan MAQBOOL, Muhammad ZIAULHAQ, Wang LIHONG, Sajid USMAN, Adnan RASHEED, Muhammad U. HASSAN, Mohamed HASHEM, Rehab O. ELNOUR, Muhammad M. IQBAL, Muhammad ARSHAD
{"title":"The critical role of nitrogen in plants facing the salinity stress: Review and future prospective","authors":"Muhammad T. ASLAM, Imran KHAN, Muhammad U. CHATTHA, Rizwan MAQBOOL, Muhammad ZIAULHAQ, Wang LIHONG, Sajid USMAN, Adnan RASHEED, Muhammad U. HASSAN, Mohamed HASHEM, Rehab O. ELNOUR, Muhammad M. IQBAL, Muhammad ARSHAD","doi":"10.15835/nbha51313347","DOIUrl":"https://doi.org/10.15835/nbha51313347","url":null,"abstract":"Salinity stress is a serious abiotic stress that negatively affect the crop growth and development. Mineral nutrient supplementation is considered as an effective strategy to mitigate the adverse effects of salinity. Nitrogen (N) is an important nutrient needed for plants and its application also an effective strategy to mitigate adverse impacts of salinity. Salinity stress disturbs plant physiological, and biochemical functions, antioxidant activities, cellular membranes, antioxidant activities and nutrient uptake thereby cause significant reduction in plant growth and development. The application of N maintains membrane stability, plant water relations, leaf gas exchange characteristics, and protect the plants from oxidative damages which induce the salt tolerance in plants. Besides, this N also improves nutrient uptake and it also induce cellular signaling that mitigate the adverse impacts of salinity. Therefore, it is interesting to understand the role of N in inducing salt tolerance in plants. In present review the mechanisms of N uptake and assimilation in plants under saline conditions are discussed. The present review provides information on how N mitigates ionic toxicity, and oxidative damages and maintains nutrient balance to counter the toxic effects of salinity stress in plants. This review will help the readers to learning more about the role of N in inducing salt tolerance in plants.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135426013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Usman SHAFQAT, Awais MAQSOOD, Aneeza ISHFAQ, Sadia MUSTAFA, Yumna RASHEED, Faisal MAHMOOD, Muhammad U. HASSAN, Jameel Mohammed AL-KHAYRI, Mohammed Ibrahim ALDAEJ, Muhammad Naeem SATTAR, Adel Abdel-Sabour REZK, Mustafa Ibrahim ALMAGHASLA, Wael Fathi SHEHATA, Tarek Abdelaziz SHALABY
{"title":"Green nanotechnology for plant bacterial diseases management in cereal crops: a review on metal-based nanoparticles","authors":"Usman SHAFQAT, Awais MAQSOOD, Aneeza ISHFAQ, Sadia MUSTAFA, Yumna RASHEED, Faisal MAHMOOD, Muhammad U. HASSAN, Jameel Mohammed AL-KHAYRI, Mohammed Ibrahim ALDAEJ, Muhammad Naeem SATTAR, Adel Abdel-Sabour REZK, Mustafa Ibrahim ALMAGHASLA, Wael Fathi SHEHATA, Tarek Abdelaziz SHALABY","doi":"10.15835/nbha51313333","DOIUrl":"https://doi.org/10.15835/nbha51313333","url":null,"abstract":"Cereals are an important source of nutrients for animals. Several diseases cause severe yield loss in cereal crops. Bacterial diseases result in varying yield losses across cereals: Wheat (5-40%), maize (15-98.9%), rice (20-70%), pearl millet (3-35%), and oats (15-49%). Diseases may be bacterial diseases, fungal or viral. Bacterial diseases are traditionally treated by pesticides. Chemically synthesized pesticides are toxic and hazardous to the environment. Nanotechnology is emerging and novel field for agriculture, especially in plant pathology as a strong antimicrobial agent. Nanoparticles have been synthesized in various ways i.e., biological, physical, and chemical methods. Chemical and physical methods of nanoparticles are costly and toxic to the environment. The biological method for the synthesis of nanoparticles is eco-friendly and economical. Microorganisms or plant extracts are used for metal nanoparticle synthesis. The application of nanoparticles in agriculture has a wide scope and it can bring nano-revolution. This review summarizes the antibacterial activity of biosynthesized metal nanoparticles and their role in bacterial disease management of cereals.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135586334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Haocheng WANG, Binjuan YANG, Haiying TANG, Athar MAHMOOD, Rizwan MAQBOOL, Muhammad U. HASSAN, Tahir A. KHAN, Muhammad M. IQBAL, Guoqin HUANG
{"title":"Winter cropping improves yield, dry matter accumulation and translocation and nitrogen uptake of double-cropping rice","authors":"Haocheng WANG, Binjuan YANG, Haiying TANG, Athar MAHMOOD, Rizwan MAQBOOL, Muhammad U. HASSAN, Tahir A. KHAN, Muhammad M. IQBAL, Guoqin HUANG","doi":"10.15835/nbha51313299","DOIUrl":"https://doi.org/10.15835/nbha51313299","url":null,"abstract":"Winter cropping is widely considered as an effective way to increase rice yield. Therefore, this study was performed to find optimal winter cropping patterns in middle and lower reaches of the Yangtze River, China. Five experimental treatments were set up based on the long-term field experiment including winter fallow (CK), Chinese milk vetch winter cropping pattern (CRR), rapeseed winter cropping pattern (RRR), garlic winter cropping pattern (GRR), and winter multiple cropping rotation (ROT). The effects of different winter cropping patterns on the yield, dry matter accumulation and translocation, and plant nitrogen uptake were explored in double-cropping rice. The results showed that compared with CK, winter cropping increased the early and late rice yield by 7.91-10.70% and 3.57-6.89%, respectively. Similarly, compared with CK, winter cropping patterns also increased the number of spikes in early rice by 19.36-25.81% and CRR and ROT increased the effective number of spikes in late rice by 25.58% and 23.26% respectively. The dry matter (DM) translocation by stem and leaf of early rice under CRR was highest, with a 36.2% increase compared with CK, however, DM accumulation after heading in early rice under CRR was lowest. GRR decreased the dry matter translocation by stem and leaf in early rice but increased DM accumulation after heading in early and late rice by 65.28% and 13.44% as compared to CK. Moreover, ROT increased the dry matter translocation by stem and leaf in late rice by 112.63%. Additionally, GRR and ROT treatments increased the stem nitrogen uptake in early rice by 61.76% and 58.61% as compared to control, while CRR increased the nitrogen uptake by stem, leave and spike in late rice by 40.76%, 49.51% and 42.92%. In conclusion, CRR is more beneficial to increase DM accumulation and translocation by stem and leaf in double-cropping rice, and nitrogen uptake by rice plants.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135586478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Molecular characterization of guava Psidium guajava in Cereté, Córdoba, Colombia","authors":"Enrique PARDO, Karen HERNÁNDEZ, Teodora CAVADÍA","doi":"10.15835/nbha51313305","DOIUrl":"https://doi.org/10.15835/nbha51313305","url":null,"abstract":"Guava (Psidium guajava L.) is the most cherished cultivated fruit species in the Myrtaceae family, and it is a perennial tree native to tropical America. The objective of this study was to determine the genetic variability of Psidium guajava in Cereté (Colombia) using SSR markers. DNA extraction was performed using the Mini-prep method with modifications. Nine microsatellites were amplified using the PCR Touchdown technique. Genetic-population parameters such as the number of alleles, effective number of alleles, observed heterozygosity, expected heterozygosity, fixation index, Hardy-Weinberg equilibrium, and polymorphic information content were calculated using PopGene 1.31 software. The number of alleles varied between 4 for markers mPgCIR13, mPgCIR20, mPgCIR23, and 8 for marker mPgCIR19, respectively. The average value of effective number of alleles was 3.722, observed heterozygosity was 0.217, and expected heterozygosity was 0.254. The average fixation index was 0.101. Hardy-Weinberg equilibrium tests revealed significant differences in the markers. The FIS coefficient had an average value of 0.385, the FIT coefficient showed an average of 0.490, and the FST coefficient had a value of 0.178. Genetic distance analysis showed that Mateo Gómez was closely related to Retiro de los Indios, while Rabolargo appeared to be the most distant population. The study revealed low genetic variability within and between the populations studied, possibly, reflecting the type of asexual propagation applied in guava crops.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135720313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}