Notulae Botanicae Horti Agrobotanici Cluj-napoca最新文献_第5页

Assessment of genetic divergence in wheat lines (Triticum aestivum L.) involving biochemical and protein markers in rainfed conditions 旱作条件下小麦品系(Triticum aestivum L.)生物化学和蛋白质标记遗传分化的评估

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-28 DOI: 10.15835/nbha51313313

Mehvish RAMZAN, Wang LIHONG, Huma TARIQ, Syed F.A. GILLANI, Saima SHAFIQUE, Adnan RASHEED, Mahmoud F. SELEIMAN

{"title":"Assessment of genetic divergence in wheat lines (Triticum aestivum L.) involving biochemical and protein markers in rainfed conditions","authors":"Mehvish RAMZAN, Wang LIHONG, Huma TARIQ, Syed F.A. GILLANI, Saima SHAFIQUE, Adnan RASHEED, Mahmoud F. SELEIMAN","doi":"10.15835/nbha51313313","DOIUrl":"https://doi.org/10.15835/nbha51313313","url":null,"abstract":"Wheat (Triticum aestivum L.) is the important and strategic cereal crop for the majority of world’s populations. It is significant staple food of about two billion people. In next few years, world demand for wheat is expected to be 40 percent higher than that of its level today. Keeping in view the importance of the crop research work was conducted in the laboratory of Plant Breeding and Molecular Genetics, University of Poonch Rawalakot, AJK, Pakistan. The aim of the research was to find out the genetic diversity of different wheat lines. In the experiment, 50 different lines of wheat species (Triticum aestivum L.) was used to detect genetic diversity by utilizing Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and biochemical analysis. On the basis of biochemical analysis lines 3111 and 3123 was diverse among 50 lines for antioxidant activity by using DPPH radical and 3135 and 3139 for phenolic contents and for flavonoid 3148 and 3107 was found more promising. Molecular characterization by SDS PAGE showed diversity in three wheat lines 3136, 3138 and 3110. These wheat lines could be our potential lines for future wheat improvement program as they were also promising regarding to the high yields.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135426018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The critical role of nitrogen in plants facing the salinity stress: Review and future prospective 氮在植物盐度胁迫中的关键作用:综述与展望

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-28 DOI: 10.15835/nbha51313347

Muhammad T. ASLAM, Imran KHAN, Muhammad U. CHATTHA, Rizwan MAQBOOL, Muhammad ZIAULHAQ, Wang LIHONG, Sajid USMAN, Adnan RASHEED, Muhammad U. HASSAN, Mohamed HASHEM, Rehab O. ELNOUR, Muhammad M. IQBAL, Muhammad ARSHAD

{"title":"The critical role of nitrogen in plants facing the salinity stress: Review and future prospective","authors":"Muhammad T. ASLAM, Imran KHAN, Muhammad U. CHATTHA, Rizwan MAQBOOL, Muhammad ZIAULHAQ, Wang LIHONG, Sajid USMAN, Adnan RASHEED, Muhammad U. HASSAN, Mohamed HASHEM, Rehab O. ELNOUR, Muhammad M. IQBAL, Muhammad ARSHAD","doi":"10.15835/nbha51313347","DOIUrl":"https://doi.org/10.15835/nbha51313347","url":null,"abstract":"Salinity stress is a serious abiotic stress that negatively affect the crop growth and development. Mineral nutrient supplementation is considered as an effective strategy to mitigate the adverse effects of salinity. Nitrogen (N) is an important nutrient needed for plants and its application also an effective strategy to mitigate adverse impacts of salinity. Salinity stress disturbs plant physiological, and biochemical functions, antioxidant activities, cellular membranes, antioxidant activities and nutrient uptake thereby cause significant reduction in plant growth and development. The application of N maintains membrane stability, plant water relations, leaf gas exchange characteristics, and protect the plants from oxidative damages which induce the salt tolerance in plants. Besides, this N also improves nutrient uptake and it also induce cellular signaling that mitigate the adverse impacts of salinity. Therefore, it is interesting to understand the role of N in inducing salt tolerance in plants. In present review the mechanisms of N uptake and assimilation in plants under saline conditions are discussed. The present review provides information on how N mitigates ionic toxicity, and oxidative damages and maintains nutrient balance to counter the toxic effects of salinity stress in plants. This review will help the readers to learning more about the role of N in inducing salt tolerance in plants.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135426013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green nanotechnology for plant bacterial diseases management in cereal crops: a review on metal-based nanoparticles 绿色纳米技术在谷类作物细菌性病害管理中的应用:金属基纳米颗粒研究进展

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-27 DOI: 10.15835/nbha51313333

Usman SHAFQAT, Awais MAQSOOD, Aneeza ISHFAQ, Sadia MUSTAFA, Yumna RASHEED, Faisal MAHMOOD, Muhammad U. HASSAN, Jameel Mohammed AL-KHAYRI, Mohammed Ibrahim ALDAEJ, Muhammad Naeem SATTAR, Adel Abdel-Sabour REZK, Mustafa Ibrahim ALMAGHASLA, Wael Fathi SHEHATA, Tarek Abdelaziz SHALABY

{"title":"Green nanotechnology for plant bacterial diseases management in cereal crops: a review on metal-based nanoparticles","authors":"Usman SHAFQAT, Awais MAQSOOD, Aneeza ISHFAQ, Sadia MUSTAFA, Yumna RASHEED, Faisal MAHMOOD, Muhammad U. HASSAN, Jameel Mohammed AL-KHAYRI, Mohammed Ibrahim ALDAEJ, Muhammad Naeem SATTAR, Adel Abdel-Sabour REZK, Mustafa Ibrahim ALMAGHASLA, Wael Fathi SHEHATA, Tarek Abdelaziz SHALABY","doi":"10.15835/nbha51313333","DOIUrl":"https://doi.org/10.15835/nbha51313333","url":null,"abstract":"Cereals are an important source of nutrients for animals. Several diseases cause severe yield loss in cereal crops. Bacterial diseases result in varying yield losses across cereals: Wheat (5-40%), maize (15-98.9%), rice (20-70%), pearl millet (3-35%), and oats (15-49%). Diseases may be bacterial diseases, fungal or viral. Bacterial diseases are traditionally treated by pesticides. Chemically synthesized pesticides are toxic and hazardous to the environment. Nanotechnology is emerging and novel field for agriculture, especially in plant pathology as a strong antimicrobial agent. Nanoparticles have been synthesized in various ways i.e., biological, physical, and chemical methods. Chemical and physical methods of nanoparticles are costly and toxic to the environment. The biological method for the synthesis of nanoparticles is eco-friendly and economical. Microorganisms or plant extracts are used for metal nanoparticle synthesis. The application of nanoparticles in agriculture has a wide scope and it can bring nano-revolution. This review summarizes the antibacterial activity of biosynthesized metal nanoparticles and their role in bacterial disease management of cereals.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135586334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Winter cropping improves yield, dry matter accumulation and translocation and nitrogen uptake of double-cropping rice 冬播提高了双季稻产量、干物质积累转运和氮素吸收

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-27 DOI: 10.15835/nbha51313299

Haocheng WANG, Binjuan YANG, Haiying TANG, Athar MAHMOOD, Rizwan MAQBOOL, Muhammad U. HASSAN, Tahir A. KHAN, Muhammad M. IQBAL, Guoqin HUANG

{"title":"Winter cropping improves yield, dry matter accumulation and translocation and nitrogen uptake of double-cropping rice","authors":"Haocheng WANG, Binjuan YANG, Haiying TANG, Athar MAHMOOD, Rizwan MAQBOOL, Muhammad U. HASSAN, Tahir A. KHAN, Muhammad M. IQBAL, Guoqin HUANG","doi":"10.15835/nbha51313299","DOIUrl":"https://doi.org/10.15835/nbha51313299","url":null,"abstract":"Winter cropping is widely considered as an effective way to increase rice yield. Therefore, this study was performed to find optimal winter cropping patterns in middle and lower reaches of the Yangtze River, China. Five experimental treatments were set up based on the long-term field experiment including winter fallow (CK), Chinese milk vetch winter cropping pattern (CRR), rapeseed winter cropping pattern (RRR), garlic winter cropping pattern (GRR), and winter multiple cropping rotation (ROT). The effects of different winter cropping patterns on the yield, dry matter accumulation and translocation, and plant nitrogen uptake were explored in double-cropping rice. The results showed that compared with CK, winter cropping increased the early and late rice yield by 7.91-10.70% and 3.57-6.89%, respectively. Similarly, compared with CK, winter cropping patterns also increased the number of spikes in early rice by 19.36-25.81% and CRR and ROT increased the effective number of spikes in late rice by 25.58% and 23.26% respectively. The dry matter (DM) translocation by stem and leaf of early rice under CRR was highest, with a 36.2% increase compared with CK, however, DM accumulation after heading in early rice under CRR was lowest. GRR decreased the dry matter translocation by stem and leaf in early rice but increased DM accumulation after heading in early and late rice by 65.28% and 13.44% as compared to CK. Moreover, ROT increased the dry matter translocation by stem and leaf in late rice by 112.63%. Additionally, GRR and ROT treatments increased the stem nitrogen uptake in early rice by 61.76% and 58.61% as compared to control, while CRR increased the nitrogen uptake by stem, leave and spike in late rice by 40.76%, 49.51% and 42.92%. In conclusion, CRR is more beneficial to increase DM accumulation and translocation by stem and leaf in double-cropping rice, and nitrogen uptake by rice plants.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135586478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular characterization of guava Psidium guajava in Cereté, Córdoba, Colombia 产自哥伦比亚ceret<e:1> Córdoba的番石榴Psidium guajava的分子特征

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-26 DOI: 10.15835/nbha51313305

Enrique PARDO, Karen HERNÁNDEZ, Teodora CAVADÍA

{"title":"Molecular characterization of guava Psidium guajava in Cereté, Córdoba, Colombia","authors":"Enrique PARDO, Karen HERNÁNDEZ, Teodora CAVADÍA","doi":"10.15835/nbha51313305","DOIUrl":"https://doi.org/10.15835/nbha51313305","url":null,"abstract":"Guava (Psidium guajava L.) is the most cherished cultivated fruit species in the Myrtaceae family, and it is a perennial tree native to tropical America. The objective of this study was to determine the genetic variability of Psidium guajava in Cereté (Colombia) using SSR markers. DNA extraction was performed using the Mini-prep method with modifications. Nine microsatellites were amplified using the PCR Touchdown technique. Genetic-population parameters such as the number of alleles, effective number of alleles, observed heterozygosity, expected heterozygosity, fixation index, Hardy-Weinberg equilibrium, and polymorphic information content were calculated using PopGene 1.31 software. The number of alleles varied between 4 for markers mPgCIR13, mPgCIR20, mPgCIR23, and 8 for marker mPgCIR19, respectively. The average value of effective number of alleles was 3.722, observed heterozygosity was 0.217, and expected heterozygosity was 0.254. The average fixation index was 0.101. Hardy-Weinberg equilibrium tests revealed significant differences in the markers. The FIS coefficient had an average value of 0.385, the FIT coefficient showed an average of 0.490, and the FST coefficient had a value of 0.178. Genetic distance analysis showed that Mateo Gómez was closely related to Retiro de los Indios, while Rabolargo appeared to be the most distant population. The study revealed low genetic variability within and between the populations studied, possibly, reflecting the type of asexual propagation applied in guava crops.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135720313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

GIS based soil erosion assessment using the USLE model for efficient land management: A case study in an area with diverse pedo-geomorphological and bioclimatic characteristics 基于GIS的土壤侵蚀评估，利用USLE模型进行有效的土地管理:以具有不同土壤地貌和生物气候特征的地区为例

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-26 DOI: 10.15835/nbha51313263

Paul SESTRAS, Sevastel MIRCEA, Sanda ROȘCA, Ștefan BILAȘCO, Tudor SĂLĂGEAN, Lucian O. DRAGOMIR, Mihai V. HERBEI, Simion BRUMA, Catalin SABOU, Rastko MARKOVIĆ, Shuraik KADER

{"title":"GIS based soil erosion assessment using the USLE model for efficient land management: A case study in an area with diverse pedo-geomorphological and bioclimatic characteristics","authors":"Paul SESTRAS, Sevastel MIRCEA, Sanda ROȘCA, Ștefan BILAȘCO, Tudor SĂLĂGEAN, Lucian O. DRAGOMIR, Mihai V. HERBEI, Simion BRUMA, Catalin SABOU, Rastko MARKOVIĆ, Shuraik KADER","doi":"10.15835/nbha51313263","DOIUrl":"https://doi.org/10.15835/nbha51313263","url":null,"abstract":"The complex environmental process of soil erosion is crucial to sustainable land management and conservation. This study uses the Universal Soil Loss Equation (USLE) model to understand the intricate interactions that cause soil erosion in Cluj County, Romania, a region susceptible to complex erosion and landslide phenomenon. The established analysis depicts erosion patterns from localised hotspots to regions of relative stability, providing valuable insights into this critical issue. Spatial distribution maps with color-coded gradients show soil erosion risk and identify vulnerable regions, with temporal investigations depicting how environmental changes affect soil erosion, making it relevant to soil conservation and land management. A careful analysis of the USLE model's parameters (Ls, Cs, C, S, K) shows their soil erosion contributions. The erosion rates were graded in five classes in accordance to general practices of USLE modelling, which range from very-low, low, moderate, high and very-high susceptibility to soil erosion. While a significant majority of the county’s surface is represented by very-low and low erosion risk, several hotspots were identified with intense erosion processes that highlights the critical need to implement soil conservation measures in the area. Identifying erosion hotspots and conservation solutions encourages stakeholders to protect soils, and can provide policymakers with useful information for developing improved guidelines for soil and water conservation.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135720312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biocontrol of Fusarium wilt disease in pepper plant by plant growth promoting Penicillium expansum and Trichoderma harzianum 利用植物生长促进青霉和哈茨木霉对辣椒枯萎病的生物防治

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-26 DOI: 10.15835/nbha51313302

Amer M. ABDELAZIZ, Mohamed H. SHARAF, Amr H. HASHEM, Abdulaziz A. AL-ASKAR, Samy A. MAREY, Fares A. MOHAMED, Mohamed N. ABDELSTAR, Mosad A. ZAKI, Hamada ABDELGAWAD, Mohamed S. ATTIA

{"title":"Biocontrol of Fusarium wilt disease in pepper plant by plant growth promoting Penicillium expansum and Trichoderma harzianum","authors":"Amer M. ABDELAZIZ, Mohamed H. SHARAF, Amr H. HASHEM, Abdulaziz A. AL-ASKAR, Samy A. MAREY, Fares A. MOHAMED, Mohamed N. ABDELSTAR, Mosad A. ZAKI, Hamada ABDELGAWAD, Mohamed S. ATTIA","doi":"10.15835/nbha51313302","DOIUrl":"https://doi.org/10.15835/nbha51313302","url":null,"abstract":"Plant growth promoting fungi (PGPF) were employed in the present study to biocontrol Fusarium wilt disease in pepper plants. Two of the five fungal isolates were chosen based on biochemical characteristics such as their production of hydrocyanic acid, siderophores, and IAA, phosphate solubilization, and in vitro antifungal activities. The most potent fungal isolates were identified as Penicillium expansum (P. expansum) and Trichoderma harzianum (T. harzianum). Using GC-MS, it was found that PGPF extracts contain compounds with antifungal activity, antioxidants, and plant growth stimulators. The combined effect of T. harzianum and P. expansum increased the protection against fusarial wilt by (76.74%), followed by T. harzianum by (50%), then P. expansum by (17.64%). Significant improvement because of using the mixture (T. harzianum and P. expansum) showed an increase in shoot length (59.4%), root length (129%), and number of leaves (52.6%). Chlorophyll A and B levels in infected plants were consistently raised by 28.71% and 67.58%, respectively; as a result of application the mixture (T. harzianum and P. expansum). Also, there was an increase in soluble proteins and carbohydrates in infected plants treated with (T. harzianum) by 25.42% and 31.78% over untreated infected plants, respectively. It could be recommended that the use of targeted PGPF strains, especially a mixture of T. harzianum and P. expansum could be commercially used as therapeutic nutrients against Fusarium wilt of pepper plants.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135720314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological responses and adoptive mechanisms in oat against three levels of salt stress 燕麦对三种盐胁迫的生理反应及适应机制

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-26 DOI: 10.15835/nbha51313249

Zhan-Wu GAO, Sun PEILIANG, Yan-Hui CUI, Asma HANIF, Zong-Ze YANG, Rui-Li LI, Chun-Sheng MU, Adnan RASHEED

{"title":"Physiological responses and adoptive mechanisms in oat against three levels of salt stress","authors":"Zhan-Wu GAO, Sun PEILIANG, Yan-Hui CUI, Asma HANIF, Zong-Ze YANG, Rui-Li LI, Chun-Sheng MU, Adnan RASHEED","doi":"10.15835/nbha51313249","DOIUrl":"https://doi.org/10.15835/nbha51313249","url":null,"abstract":"To identify the propagation mechanisms and the adaptive strategies of oat seedlings exposed to NaCl, NaHCO3 and Na2CO3 the main salts in the soils of the salt-alkali grasslands of the Songnen Plain of China, growth rates and physiological indices of oat seedlings were measured in plants grown in soils with different concentrations (48-144 mmol L-1) of the three salts. The results demonstrated that although oat seedlings survival rates were unaffected by NaCl stress, the tiller number, plant height, and shoot and root dry weights decreased with increasing salt concentration, in the order of Na2CO3＞NaHCO3＞NaCl. In addition, propagation mechanisms higher concentrations of Na+ accumulated in the shoots and roots of oat seedlings under Na2CO3 stress and NaHCO3 stress than in seedlings under NaCl stress. Reductions in concentrations of K+ were also greater under both Na2CO3 and NaHCO3 stress than NaCl, especially in the roots. Large amounts of Cl－ and proline were found to accumulate in oat seedlings, most likely as a strategy for maintaining osmotic and ionic homeostasis under NaCl stress.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135720531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic diversity and population structure of tomato brown rugose fruit virus (ToBRFV) variants from Antalya province, Turkey 土耳其安塔利亚省番茄褐皱果病毒(ToBRFV)变异的遗传多样性和群体结构

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-25 DOI: 10.15835/nbha51313356

Abdullah GÜLLER, Mustafa USTA, Filiz RANDA-ZELYÜT

{"title":"Genetic diversity and population structure of tomato brown rugose fruit virus (ToBRFV) variants from Antalya province, Turkey","authors":"Abdullah GÜLLER, Mustafa USTA, Filiz RANDA-ZELYÜT","doi":"10.15835/nbha51313356","DOIUrl":"https://doi.org/10.15835/nbha51313356","url":null,"abstract":"In this study, bioinformatic analyses were carried out according to the fully coded CP and MP gene regions of the agent, using six novel tomato brown rugose fruit virus (ToBRFV) variants obtained from the production greenhouses in Antalya, where the infection was first detected in Turkey and global variants. Molecular evolutionary analyses using both CP and MP gene regions showed that all variants were distributed in three major clades. Population dynamics studies for both gene regions have shown that there was very low nucleotide diversity and haplotype diversity. The low haplotype diversity for the CP and MP genes indicated almost no recombination status. A strong negative selection was determined for CP and MP gene regions, dN/dS= 0.0877 and dN/dS=0.2104, respectively. Neutrality test results revealed that ToBRFV populations are in an expansion phase. Pairwise comparisons were performed between populations separated in the geographic hierarchy as American, European, and Asian variants, and the findings showed intense gene flow and high genetic similarity (FST<0,33 and migration rate >1). The results of this study reveal the recent population structure of the virus and suggest that necessary precautions should be taken in the international seed trade against contaminated seeds.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135866277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The growth rate and genetic variability of Scots pine (Pinus sylvestris L.) half-sibs in test crops of Northern Kazakhstan 北哈萨克斯坦试验作物中苏格兰松(Pinus sylvestris L.)半兄弟的生长速率和遗传变异

4区生物学

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-25 DOI: 10.15835/nbha51313261

Yana KREKOVA, Nadezhda CHEBOTKO, Dmitry KAGAN, Svetlana IVANOVSKAYA, Yekaterina VIBE, Andrey KABANOV

{"title":"The growth rate and genetic variability of Scots pine (Pinus sylvestris L.) half-sibs in test crops of Northern Kazakhstan","authors":"Yana KREKOVA, Nadezhda CHEBOTKO, Dmitry KAGAN, Svetlana IVANOVSKAYA, Yekaterina VIBE, Andrey KABANOV","doi":"10.15835/nbha51313261","DOIUrl":"https://doi.org/10.15835/nbha51313261","url":null,"abstract":"Scots pine (Pinus sylvestris L.) is a valuable tree species, playing an important role in maintaining the ecological and economic balance not only in Kazakhstan, but also in many countries around the world. The purpose of this study was to assess the growth rate and to identify the genotypes of the half-sib progeny of Scotch pine plus trees in order to further obtain high-quality seeds with valuable genetic traits. A molecular genetic analysis was carried out using six microsatellite and 20 isoenzyme loci on 15 Scots pine half-sib families, represented by two samples of seeds collected in the subsequent harvesting periods (planted in 1986 and 1987), that evaluated the taxonomic characteristics such as tree height and trunk diameter (DBH, measured at 1.3 meters). Families with the highest growth rate in both samples were selected as candidates for the elite group. During the SSR analysis, a decrease in the level of expected heterozygosity (0.2377-0.5362 with an average value of 0.4593) was observed in P. sylvestris L. half-sibs compared to the sample of maternal plus trees (0.5444), with similar results to that of the “founder effect”. At the same time, the level of observed heterozygosity remained sufficiently high (0.3333-0.7037 with an average value of 0.4980 and 0.4556, respectively). During the isoenzyme analysis, the level of genetic variability either decreased or was similar to that of the maternal trees. Rare and unique alleles were found in seed samples of the analysed tree families. In general, this research showed that a comprehensive evaluation combining both traditional breeding methods (genetic assessment of progeny in the course of growth) and molecular genetic analysis is required for the selection of elite trees.","PeriodicalId":19364,"journal":{"name":"Notulae Botanicae Horti Agrobotanici Cluj-napoca","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135866265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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