Molecular characterization of guava Psidium guajava in Cereté, Córdoba, Colombia

IF 1.4 4区生物学 Q3 PLANT SCIENCES

Notulae Botanicae Horti Agrobotanici Cluj-napoca Pub Date : 2023-09-26 DOI:10.15835/nbha51313305

Enrique PARDO, Karen HERNÁNDEZ, Teodora CAVADÍA

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引用次数: 0

Abstract

Guava (Psidium guajava L.) is the most cherished cultivated fruit species in the Myrtaceae family, and it is a perennial tree native to tropical America. The objective of this study was to determine the genetic variability of Psidium guajava in Cereté (Colombia) using SSR markers. DNA extraction was performed using the Mini-prep method with modifications. Nine microsatellites were amplified using the PCR Touchdown technique. Genetic-population parameters such as the number of alleles, effective number of alleles, observed heterozygosity, expected heterozygosity, fixation index, Hardy-Weinberg equilibrium, and polymorphic information content were calculated using PopGene 1.31 software. The number of alleles varied between 4 for markers mPgCIR13, mPgCIR20, mPgCIR23, and 8 for marker mPgCIR19, respectively. The average value of effective number of alleles was 3.722, observed heterozygosity was 0.217, and expected heterozygosity was 0.254. The average fixation index was 0.101. Hardy-Weinberg equilibrium tests revealed significant differences in the markers. The FIS coefficient had an average value of 0.385, the FIT coefficient showed an average of 0.490, and the FST coefficient had a value of 0.178. Genetic distance analysis showed that Mateo Gómez was closely related to Retiro de los Indios, while Rabolargo appeared to be the most distant population. The study revealed low genetic variability within and between the populations studied, possibly, reflecting the type of asexual propagation applied in guava crops.

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产自哥伦比亚ceret Córdoba的番石榴Psidium guajava的分子特征

番石榴(Psidium guajava L.)是番石榴科中最珍贵的栽培水果，是一种原产于热带美洲的多年生乔木。本研究的目的是利用SSR标记确定哥伦比亚ceret番石榴的遗传变异。DNA提取采用修改后的Mini-prep方法。采用PCR触地技术扩增9颗微卫星。利用PopGene 1.31软件计算等位基因数、有效等位基因数、观察杂合度、期望杂合度、固定指数、Hardy-Weinberg平衡、多态信息含量等遗传种群参数。标记mPgCIR13、mPgCIR20、mPgCIR23的等位基因数量分别为4个和mPgCIR19的8个。有效等位基因数平均值为3.722，观察杂合度为0.217，期望杂合度为0.254。平均固定指数为0.101。Hardy-Weinberg平衡检验显示标记物存在显著差异。FIS系数平均值为0.385,FIT系数平均值为0.490,FST系数平均值为0.178。遗传距离分析表明，Mateo Gómez与Retiro de los Indios亲缘关系密切，而Rabolargo似乎是距离最远的种群。研究表明，所研究群体内部和群体之间的遗传变异性较低，这可能反映了番石榴作物无性繁殖的类型。

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来源期刊

Notulae Botanicae Horti Agrobotanici Cluj-napoca PLANT SCIENCES-

CiteScore

2.70

自引率

0.00%

发文量

118

审稿时长

3 months

期刊介绍： Notulae Botanicae Horti Agrobotanici Cluj-Napoca is a peer-reviewed biannual journal aimed at disseminating significant research and original papers, critical reviews and short reviews. The subjects refer on plant biodiversity, genetics and plant breeding, development of new methodologies that can be of interest to a wide audience of plant scientists in all areas of plant biology, agriculture, horticulture and forestry. The journal encourages authors to frame their research questions and discuss their results in terms of the major questions of plant sciences, thereby maximizing the impact and value of their research, and thus in favor of spreading their studies outcome. The papers must be of potential interest to a significant number of scientists and, if specific to a local situation, must be relevant to a wide body of knowledge in life sciences. Articles should make a significant contribution to the advancement of knowledge or toward a better understanding of existing biological and agricultural concepts. An international Editorial Board advises the journal. The total content of the journal may be used for educational, non-profit purposes without regard to copyright. The distribution of the material is encouraged with the condition that the authors and the source (Notulae Botanicae Horti Agrobotanici Cluj-Napoca or JCR abbrev. title Not Bot Horti Agrobo) are mentioned.