R. Kachkoul, Ghita Benjelloun Touimi, Ghita El Mouhri, R. El Habbani, A. Lahrichi
{"title":"Pathophysiological aspects of renal stone formation and stone types","authors":"R. Kachkoul, Ghita Benjelloun Touimi, Ghita El Mouhri, R. El Habbani, A. Lahrichi","doi":"10.55779/nsb15111462","DOIUrl":"https://doi.org/10.55779/nsb15111462","url":null,"abstract":"Urinary stone formation is one of the oldest and most widespread diseases known to man. The disease has a multifactorial etiology that includes anatomic, environmental, genetic, infectious, metabolic, nutritional, and most importantly socio-economic factors. It is caused by a biochemical imbalance in urine between stone-forming inhibitors and promotors in a process known as lithogenesis. The mechanisms underlying the formation and development of urinary stones are not fully understood, but it can be said that they generally begin by increased urinary supersaturation of lithiasis promoters in the urine, followed by nucleation and aggregation. Subsequently, the crystals combine with other crystals in solution to form agglomerates that accumulate in the kidney. Free radical-mediated oxalate-induced renal membrane damage promotes crystal retention at the surface of the renal papilla, as well as crystal nucleation at lower supersaturation levels. In addition, stone type identification is of great interest in guiding the physician to an effective diagnosis, which allows to determine the causes in order to treat the disease and prevent recurrence. In this context, this present study reviews current knowledge on the pathophysiological aspects of kidney stone formation as well as the type of stones.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41745801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali El Boukhari, Salma Tabi, A. El Mousadik, Rachida El Boullani, A. Tahiri, Meriyem Koufan, H. Benyahia, R. Bouharroud, Naïma Ait Aabd
{"title":"Flow cytometry and chromosome numbers variation in argan tree Argania spinosa (L.) Skeels","authors":"Ali El Boukhari, Salma Tabi, A. El Mousadik, Rachida El Boullani, A. Tahiri, Meriyem Koufan, H. Benyahia, R. Bouharroud, Naïma Ait Aabd","doi":"10.55779/nsb15111451","DOIUrl":"https://doi.org/10.55779/nsb15111451","url":null,"abstract":"Argania spinosa L. Skeels is an endemic species of west-central Morocco, which is characterized by a high diversity of morphological and genetic traits. It constitutes a natural resource for oleo-agro-sylvo-pastoral uses. All conservation and genetic breeding strategies aimed to domesticate argan require a good knowledge of the plant material. However, several studies focused on agronomical, morphological, phytochemical, and molecular characterization, while the cytogenetic aspects were less investigated. The objective of this work is to identify the chromosome number and ploidy level on the national argan collection at the Agadir Regional Agronomic Research Center, Morocco. The determination of the chromosome number was carried out on root tips of germinated seeds collected from five trees genotypes selected on various morphological aspects. As a result, chromosome count on active root tip cells showed variation in the number (2n = 20; 2n = 22; 2n = 24) with a stable ploidy level (2n = 2x) that is confirmed by flow cytometry. These results combine two previous findings (2n=20, 2n=24) and reveal a third existence of twenty-two chromosome. As a conclusion, A. spinosa has three chromosomal numbers which represent the genetic diversity of the chromosomal number that this species exhibits. More studies are required to explain this variation on chromosome numbers for future breeding programs and to avoid incompatibilities.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46596171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antagonism and plant growth promoting traits of actinomycetes isolated from the rhizosphere of halophyte Atriplex halimus L.","authors":"Inas Boukelloul, Lamia Aouar, Mohamed CHEKARA BOUZIANI, A. Zellagui, Mouna Derdour, Youcef Necib","doi":"10.55779/nsb15111437","DOIUrl":"https://doi.org/10.55779/nsb15111437","url":null,"abstract":"Biocontrol is considered as an effective alternative to the application of agrochemicals, which are harmful to the environment, human, and animal health. In this study, twenty-six strains of actinomycetes were isolated from rhizospheric arid soil of the halophyte Atriplex halimus L. ῾Guettaf’ in Biskra province, Algeria. The six isolates that have inhibited at least three phytopathogenic fungi among the five tested (Fusarium oxysporum, Alternaria alternata, Fusarium solani, Aspergillus flavus and Botrytis cinerea) were selected, and have been tested in vitro against phytopathogenic bacteria (Pectobacterium carotovorum and Streptomyces scabies). They were also evaluated for their ability to hydrolyze phosphate, elaborate siderophores, produce indole-3-acetic acid (IAA), and to antagonize S. scabies in vivo (on radish seedlings). Based on the physicochemical analyses, soil samples were categorized as alkaline and extremely-saline. The antagonism results revealed varying antifungal potential among the selected isolates (Act11, Act16, Act17, Act18, Act23 and Act24), about 50% were able to inhibit the growth of F. solani and A. flavus, followed by 33.33% of those having antagonized F. oxysporum, while A. alternata was found to be the most sensitive. Only Act18 has antagonized S. scabies in vitro with an inhibition diameter zone of 19 ± 0.41 mm. However, in vivo trials showed that four isolates have counteracted S. scabies. Among them, Act18 and Act24 have significantly and positively affected the root surface (P = 0.0062) and prevented common scab. IAA was detected in all selected isolates with Act24 being the highest producer (77.45 μg mL−1). Additionally, degradation ability revealed that four isolates were able to hydrolyze phosphate while three exhibited the capacity of elaborating siderophores. The six isolates were assigned to Streptomyces genius according to their morphological, physiological and chemotaxonomical traits. Based on this study, Streptomyces sp. Act18 and Streptomyces sp. Act24 that tolerate 7.5% NaCl concentration, prevent common scab and exhibit some plant growth attributes, may be considered as promising biocontrol agents to be applied in arid and saline soils.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46146019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Anatomical and morphological structure of the leaf of the genus Malus spp.","authors":"I. Goncharovska, I. Szot","doi":"10.55779/nsb15111449","DOIUrl":"https://doi.org/10.55779/nsb15111449","url":null,"abstract":"The article describes changes in the structure, leaves, (including mesophyll structure, structure of the midrib, distribution of stomata, and the number of vascular bundles) in Malus during the growing season (from June to August) under the influence of prolonged drought. The leaves anatomy and morphology were investigated by methods of light and scanning electron microscopy. The study was carried out on four representatives of the genus Malus: M. niedzwetzkyana Borkh., M. baccata ‘Pendula’, M. domestica ‘Eliza Rathke’, M. domestica ‘Renet Simirenko’. The influence of drought on the size of the mesophyll of the leaf plate and the number of stomata along the stretch from June to August was studied. The cell circumference of the sclerenchyma ranged from μm in 27.33 (M. niedzwetzkyana) to 81.92 μm (in M. domestica ‘Elise Rathke’). The number of stomata varied from 214.0 units in (M. domestica ‘Renet Simirenko’) to 304.0 units in (M. baccata ‘Pendula’), length from 11.6 μm (M. baccata ‘Pendula’) to 28.63 μm (M. domestica ‘Elise Rathke’), width from 9.4 μm (M. domestica ‘Elise Rathke’) up to 21.8 μm (M. domestica ‘Elise Rathke’) μm. The length of the trichomes varied from 409.92 μm (M. domestica ‘Renet Simirenko’) to 745.09 μm (M. niedzwetzkyana). Also, the anocytic stomatal type, the number, different stomata and trichomes, the presence of wax on the cuticle, and the found druses of calcium oxalate in M. baccata ‘Pendula’, can be important for systematic phylogeny. Thanks to these studies, it is possible to suggest physiological adaptability of apple trees to prolonged drought, as well as to identify more resistant phenotypes for the breeding process to develop resistant cultivars.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45754782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anas EL MEKKAOUI, M. Khamar, C. Slimani, A. Nounah, E. Cherkaoui, F. Benradi, Chaimae Rais
{"title":"Phytochemical studies and in vitro evaluation of the antioxidant activity of some medicinal and aromatic plants from Morocco","authors":"Anas EL MEKKAOUI, M. Khamar, C. Slimani, A. Nounah, E. Cherkaoui, F. Benradi, Chaimae Rais","doi":"10.55779/nsb15111423","DOIUrl":"https://doi.org/10.55779/nsb15111423","url":null,"abstract":"The present work was carried out to evaluate the phenolic compounds and the antioxidant activities of some solvent extract (methanol, hydroethanol and aqueous) of several Moroccan medicinal plants known for their high antioxidant properties. The extracts were obtained by sonication, then, the total phenolics and flavonoids compounds were determined using Folin-Ciocalteu and Aluminium chloride. Afterwards, the Total Antioxidant Capacity and DPPH scavenging methods were performed. Results of phytochemical analysis showed that the total phenolics content were the highest in the hydroethanolic extract of Arbutus unedo with 160.76 mg GAE g-1DM, and the flavonoids content were the highest for the hydroethanolic extracts of Inula viscosa with 489.77 mg QE g-1 DM. Also, it can be noted that Arbutus unedo, Argania spinosa, and Myrtus communis exhibited the most potent antioxidant activity respectively with 0.026; 0.043; 0.036 mg ml-1.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45443922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"FTIR analysis and study of some physicochemical parameters and antioxidant activity of Opuntia ficus indica seed oil from Tebessa region, Algeria","authors":"Mouna Dib, A. Benbott","doi":"10.55779/nsb15111345","DOIUrl":"https://doi.org/10.55779/nsb15111345","url":null,"abstract":"Opuntia ficus-indica is well-known in Algeria for its nutritional and therapeutic properties. Our study aims to determine the physicochemical properties, the functional groups and the antioxidant activity of cactus seed oil and thus contribute to the valorization of seeds. The extraction of prickly pear seed oil with cold-press provided a yield of 4.26± 0.93%. The evaluation of the its physicochemical parameters by the norms of AFNOR yielded the following results: density: 0.908± 0.00030 kg/dm; RI: 1.4714±0,00010; AV: 1.4± 0.09 mg KOH/g; IV: 114.08± 0.67 g I 2 /100 g; SV: 188.94± 0.58 mg KOH / g; ES: 187.54±0.49 mg KOH/g; PV: 1.56 g O2 /g; chlorophyll number: 1.076 and Carotenoids number: 0.315. FTIR spectrum was used to determine the functional groups and type of associated vibrations in the extract oil, which was scanned in the range (4000-400) cm -1. The FTIR results showed that the oil extract contains fatty acid methyl esters, revealing functional groups with ranges of properties, H- C = O, -(CH2) n -, C-O, C = C and C = O in the spectrum. The analysis of antioxidant activity using DPPH and FRAP methods showed that the OFI seed oil had a significant activity in capturing free radicals (IC50= 0.050 mg/mL), (EC50 = 0.123 mg/mL) respectively. We conclude from this current study that this oil can be used in folk medicine.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49243788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. R. Baiyeri, K. Yusuf, R. O. Obalowu, Ganiyu Saad, I. Banjoko
{"title":"Impact of magnetization of irrigation water on growth, yield and nutritional qualities of tomato under deficit irrigation","authors":"M. R. Baiyeri, K. Yusuf, R. O. Obalowu, Ganiyu Saad, I. Banjoko","doi":"10.55779/nsb15111360","DOIUrl":"https://doi.org/10.55779/nsb15111360","url":null,"abstract":"Tomato is a vegetable crop commonly grown in Nigeria and consumed by most people. It contains vitamins and vital nutrients that are essential for good health. This study was conducted to determine the effect of magnetization of irrigation water on the growth, yield and nutritional qualities of tomatoes under deficit irrigation. Tomato seeds (‘Roma VF’ and ‘UC 82B’ varieties) were planted in 96 buckets (11 liters capacity), 48 buckets for magnetized water (MW) and 48 buckets for non-magnetized water (NMW), grown in a greenhouse and harvested 82 days after planting. The irrigation water was treated with 30 pieces of 10×25×50 mm neodymium magnets (1.2 Tesla). The tomato plants were subjected to deficit irrigation to determine the effect on the performance of tomatoes at 100% (1 liter), 80% (0.8 liters), 60% (0.60 liters) and 50% (0.50 liter) water requirement. Each treatment was replicated 6 times for both MW and NMW. The MW increased the growth of ‘Roma VF’ and ‘UC 82B’ varieties by 5.44-38.10% and the stem girth by 21.13-49.01%. MW increased the yield of ‘Roma VF’ variety by 110.00%, 36.00%, 6.26% and 24.00% for 100%, 80%, 60% and 50% of water application, respectively but increased the yield of ‘UC 82B’ variety by 56.52% for 100% water application. MW also improved vitamin A and C content by 7.89-27.94% and 0.45-19.06%, respectively. The concentrations of Lead were slightly higher in the tomato irrigated with MW than in the NMW but values of other heavy metals were not consistent and very close for both MW and NMW.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45368335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"From skin infection to invasive diseases: A descriptive review of Staphylococcus aureus, focusing on Panton-Valentine leucocidin and methicillin-resistant strains","authors":"I. Zeouk","doi":"10.55779/nsb15111402","DOIUrl":"https://doi.org/10.55779/nsb15111402","url":null,"abstract":"Despite advances in scientific research, Staphylococccus aureus remains a pyogenic and toxigenic bacterium involved in different infections, it endowed with the capacity to infect several biotopes and cause a wide range of infections ranging from skin diseases to other serious pathologies such as pneumonia, meningitis, sepsis, osteomyelitis and infectious endocarditis. Moreover, the emergence of resistant strains constitutes a serious public health problem. Thus, the development of new active compounds from natural sources such as medicinal plants is urgently needed. To this end, the aim of our review was to describe the state of art of infections caused by S. aureus, its pathogenesis, treatment and to provide a synthesis about studies reporting a bio guided isolation of most promising compounds selected for their anti-staphylococcal activity.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43183350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biogenesis of silver nanoparticles from the shoot extract of Delonix regia its characterization (UV–Vis spectroscopy and SEM) and evaluation for antimicrobial potential","authors":"B. Sharma, A. Alam","doi":"10.55779/nsb15111199","DOIUrl":"https://doi.org/10.55779/nsb15111199","url":null,"abstract":"The current research work explores the production of silver nanoparticles using aqueous extracts of Delonix regia (Boj. ex Hook.) Raf. (Angiosperms; Fabaceae) shoots for the bioreduction of Ag metal and its antimicrobial activity. Fourier transform infrared spectroscopy (FTIR), Zeta potential, ultraviolet-visible spectroscopy, scanning electron microscopy (SEM), and X-ray diffraction (XRD) have been used to evaluate the produced silver nanoparticles (AgNPs). Both antibacterial and antifungal activity were examined against bacterial and fungal pathogens, viz., Escherichia coli and Bacillus subtilis, and fungal strains, viz., Fusarium oxysporum and Aspergillus niger. The presence of silver nanoparticles was observed by the color change, i.e., from pale yellow to dark brown. The zeta potential observed for the produced nanoparticle is -18mv. The SEM and XRD revealed the size of synthesized AgNPs, i.e., 35nm and SEM size lies in the range of 40-60 nm. UV-visible absorption spectra were found at wavelength 425 nm. The synthesized nanoparticles are cost-efficient and could be an alternative procedure for the peculiar production of nanoparticles and also act as potential antimicrobial agents.","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42581658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Potential biological control agents for the control of vector mosquitoes: A review","authors":"Anjali Rawani","doi":"10.55779/nsb15111325","DOIUrl":"https://doi.org/10.55779/nsb15111325","url":null,"abstract":"Mosquitoes are a major cause of lethal vector-borne diseases like dengue, malaria, filariasis, chikungunya, and Japanese encephalitis, among other diseases. In a developing country like India, mosquito-borne diseases are significant threats to familiar people as in certain places, there remains low sanitation. Larval and pupal life stages of mosquitoes are mostly confined to tropical and temperate waterbodies and often form a significant proportion of biomass waterbodies. Due to rebound vectorial capacity, resistance to chemical insecticides, and harmful environmental effects, the vector control program has shifted to using biological control agents. These methods are target-specific, eco-friendly, cost-effective, and can be easily deployed. So, the present review is focused on collating and updating the information on the use of aquatic predators, bacterial strains such as Bacillus sp. and actinobacterial, algae, and fungi, which are widely used for control of adult mosquitoes in their variety of natural habitats. This review also covers the predation of larvivorous fish and botanical insecticides. ","PeriodicalId":19353,"journal":{"name":"Notulae Scientia Biologicae","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41859375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}