Neuroscience Research Communications最新文献_第10页

Differential responses of intracisternal injection of interleukin‐1 β to acute and inflammatory orofacial pain model in freely moving rats 脑内注射白介素- 1 β对自由活动大鼠急性和炎症性口面部疼痛模型的差异反应

Neuroscience Research Communications Pub Date : 2002-11-01 DOI: 10.1002/NRC.10047

H. Choi, J. S. Park, D. Ahn

{"title":"Differential responses of intracisternal injection of interleukin‐1 β to acute and inflammatory orofacial pain model in freely moving rats","authors":"H. Choi, J. S. Park, D. Ahn","doi":"10.1002/NRC.10047","DOIUrl":"https://doi.org/10.1002/NRC.10047","url":null,"abstract":"The present study was performed to investigate the modulatory role of certain interleukin (IL)-1β in nociception with a nociceptive jaw opening reflex and an orofacial formalin test in freely moving rats. In an acute pain model, 10 pg, 100 pg and 1 ng IL-1β injected intracisternally did not change the digastric eletromyogram (dEMG). However, 10 ng IL-1β suppressed dEMG to 76±8 % of control values. In an inflammatory pain model, 10 pg IL-1β injected intracisternally did not change formalin-induced noxious behavioral responses. However, 100 pg IL-1β increased formalin-induced noxious behavioral responses. At higher dose of 10 ng IL-1β, it did not increase formalin-induced behavioral responses. Pretreatment with IL-1 receptor antagonist abolished hyperalgesic response of 100 pg IL-1β. These results suggest that the intracisternal injection of IL-1β modulate the transmission of nociceptive information in the orofacial area. The hypo/hyper-algesic responses of central cytokines seem to depend on the pain model or dose related manner and the hyperalgesic action seems to be mediated by IL-1 receptor.","PeriodicalId":19198,"journal":{"name":"Neuroscience Research Communications","volume":"43 1","pages":"145-154"},"PeriodicalIF":0.0,"publicationDate":"2002-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80780023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Stimulation of Ca2+/Mg2+‐ATPase activity in adult rat cerebrocortical synaptosomes by 3‐5‐3′‐L‐triiodothyronine 3‐5‐3′‐L‐三碘甲状腺原氨酸对成年大鼠脑皮质突触体Ca2+/Mg2+‐atp酶活性的刺激

Neuroscience Research Communications Pub Date : 2002-11-01 DOI: 10.1002/NRC.10052

Nilkanta Chakrabarti, A. Ray

{"title":"Stimulation of Ca2+/Mg2+‐ATPase activity in adult rat cerebrocortical synaptosomes by 3‐5‐3′‐L‐triiodothyronine","authors":"Nilkanta Chakrabarti, A. Ray","doi":"10.1002/NRC.10052","DOIUrl":"https://doi.org/10.1002/NRC.10052","url":null,"abstract":"Stimulation of the calcium-stimulated and magnesium-dependent adenosine triphosphatase (Ca2+/Mg2+-ATPase) activity by 3-5-3′-L-triiodothyronine (T3) in synaptosomes prepared from adult rat cerebral cortex was demonstrated both in vitro and in vivo. T3 stimulated the Ca2+/Mg2+-ATPase activity in time dependent and dose dependent manner to depolarization-induced synaptosomes suspended in Ca2+-loaded choline-chloride buffer, represented involvement of the recovery phase of the changes in intrasynaptosomal Ca2+ level in this activation. T3 (100nM) also stimulated the enzyme activity in nondepolarized intact synaptosomes suspended in choline-chloride buffer. T3 had direct and dose-dependent stimulatory effect on the Ca2+/Mg2+-ATPase activity of the lysed synaptosomal suspension. The effect of T3 on the lysed synaptosomes was higher than that found on the nondepolarized intact synaptosomes. The condition probably represents the direct and/or indirect interaction of T3 with the enzyme through plasmamembrane related phenomenon that needs further investigation. After T3 treatment, a dose-dependent activation of the enzyme was found in vivo. It appears that T3 plays a role on adult brain neuronal Ca2+-mobilization through stimulation of the Ca2+/Mg2+-ATPase activity in nerve terminals.","PeriodicalId":19198,"journal":{"name":"Neuroscience Research Communications","volume":"32 1","pages":"193-201"},"PeriodicalIF":0.0,"publicationDate":"2002-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91352329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Effects of L‐arginine on the brain ischaemia‐reperfusion damage in rats: An investigation by somatosensory evoked potentials and histopathology L -精氨酸对大鼠脑缺血再灌注损伤的影响:体感诱发电位和组织病理学研究

Neuroscience Research Communications Pub Date : 2002-11-01 DOI: 10.1002/NRC.10050

T. Kurt, A. Oğuzhanoğlu, R. Ortaç, B. Turman, E. Adiguzel

引用次数: 2

Effects of low‐dose scopolamine on locomotor activity: No dissociation between cognitive and non‐effects 低剂量东莨菪碱对运动活动的影响:认知和非影响之间没有分离

Neuroscience Research Communications Pub Date : 2002-11-01 DOI: 10.1002/NRC.10049

G. Poorheidari, J. Pratt, Nima Dehghani

{"title":"Effects of low‐dose scopolamine on locomotor activity: No dissociation between cognitive and non‐effects","authors":"G. Poorheidari, J. Pratt, Nima Dehghani","doi":"10.1002/NRC.10049","DOIUrl":"https://doi.org/10.1002/NRC.10049","url":null,"abstract":"Attempts have been made to dissociate the cognitive effects of scopolamine from its non-cognitive effects. It has been suggested that low doses of scopolamine may induce memory impairment without inducing significant non-cognitive effects. We therefore tested changes in locomotor activity (as a non-cognitive effect) in rats treated with low-dose scopolamine (which is believed to induce cognitive effects only). \u0000 \u0000 \u0000 \u0000In this study, locomotor activity (as a non-cognitive effect) induced by low doses of this drug was evaluated by using two methods and rat strains. In the first study (circular box method, an automated open-field), scopolamine hydrobromide (HBr), scopolamine methylbromide (MeBr) or saline was injected subcutaneously into male Sprague-Dawley rats. After 30 min, rats were put into an automated open-field and locomotor activity was quantified by recording interruptions of infrared beams, with print-outs every 2 min for 16 min. Locomotor activity was assessed by summing these recordings. In the second study (closed platform), scopolamine HBr or saline was injected intraperitoneally into male Long-Evans rats. Twenty minutes later, the rats were placed in the center of a square-shaped closed platform (with 3×3 divisions). Locomotor activity was defined as the sum of crossings (traversing of four paws of the rat from one area into another of nine) and rears, which were recorded every 5 min for 20 min. \u0000 \u0000 \u0000 \u0000Results from the circular box study showed that scopolamine HBr produced a marked increase in locomotor activity whereas scopolamine MeBr produced a non-significant decrease in locomotor activity. The closed platform data also demonstrated that scopolamine HBr increased locomotor activity significantly. \u0000 \u0000 \u0000 \u0000These data show that scopolamine can induce non-cognitive effects (such as increased locomotor activity), even at low doses. Our results also imply that the increase in locomotor activity is mediated centrally.","PeriodicalId":19198,"journal":{"name":"Neuroscience Research Communications","volume":"37 4","pages":"165-174"},"PeriodicalIF":0.0,"publicationDate":"2002-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91535637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Intrathecal methylprednisolone does not improve outcome after severe spinal cord injury in the rat 鞘内注射甲基强的松龙不能改善大鼠严重脊髓损伤后的预后

Neuroscience Research Communications Pub Date : 2002-11-01 DOI: 10.1002/NRC.10051

I. Vanický, L. Urdzikova, K. Saganová, M. Marsala

引用次数: 10

Transplantation of a new composite of neural cells and marrow stromal cells into rat brain after stroke 一种新型神经细胞与骨髓基质细胞复合物在脑卒中后的移植研究

Neuroscience Research Communications Pub Date : 2002-11-01 DOI: 10.1002/NRC.10048

Yi Li, X. Yang, Jieli Chen, Lei Wang, Ying Wang, Chunling Zhang, Xiaoguang Chen, M. Katakowski, T. Mikkelsen, Mei Lu, M. Chopp

引用次数: 3

Hindbrain glucoprivic inhibition of the proestrus lutenizing hormone surge in the female rat is attenuated by exogenous lactate administration 雌性大鼠后脑葡萄糖对发情前黄体激素激增的抑制作用被外源性乳酸给药减弱

Neuroscience Research Communications Pub Date : 2002-09-01 DOI: 10.1002/NRC.10039

K. Briski

{"title":"Hindbrain glucoprivic inhibition of the proestrus lutenizing hormone surge in the female rat is attenuated by exogenous lactate administration","authors":"K. Briski","doi":"10.1002/NRC.10039","DOIUrl":"https://doi.org/10.1002/NRC.10039","url":null,"abstract":"Central glucose availability is a critical monitored variable in the regulation of reproductive neuroendocrine function. While current studies implicate the periventricular caudal brainstem as a source of signaling of decreased availability of catabolic intermediates and/or glycolytic end products, the mechanisms by which local metabolic ‘sensors’ signal diminished glucose utilization are not clear. The present experiments investigated whether fourth ventricular infusion of the aerobic catabolic substrate, lactate, attenuates glucoprivic suppression of luteinizing hormone (LH). Cyclic female rats were injected at 13.00h on proestrus with the glucose antimetabolite, 2-deoxy-D-glucose (2DG; 50 vs. 150ug), or vehicle into the rostral fourth ventricle. Additional proestrus animals were treated by continuous fourth ventricular infusion of 10.0 µM lactate/hr or vehicle, initiated prior to ip injection of 400mg 2DG/kg bw or saline. Serial blood samples were obtained hourly during proestrus afternoon for radioimmunoassay of plasma LH. The results demonstrate a reduction in the LH surge following icv 2DG administration, while showing that fourth ventricular infusion of lactate reversed inhibition of hormone release by systemic antimetabolite administration. These findings support the view that hindbrain signaling of glucose metabolic dysfunction initiates neural mechanisms that inhibit the proestrus LH surge, and that lactate utilization may be monitored as an indicator of local cellular energy stasis.","PeriodicalId":19198,"journal":{"name":"Neuroscience Research Communications","volume":"10 1","pages":"67-73"},"PeriodicalIF":0.0,"publicationDate":"2002-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85943767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Cyclosporine‐A delays the end‐plate degeneration in denerved rat muscles 环孢素A延缓大鼠无神经肌肉终板退变

Neuroscience Research Communications Pub Date : 2002-09-01 DOI: 10.1002/NRC.10041

L. Rodella, G. Bonaspetti, R. Rezzani, E. Borsani, R. Fenu, U. Pazzaglia, R. Bianchi

引用次数: 0

Identification of novel protein-coding genes from human fetal hippocampus ESTs database 从人胎儿海马ESTs数据库中鉴定新的蛋白质编码基因

Neuroscience Research Communications Pub Date : 2002-09-01 DOI: 10.1002/NRC.10042

Keyue Ding, Xiaojing Sun, Min Zhou, Jing Cai, Tianjing Cai, Yan Zhang, Tongbin Zhu, Zhengguo Zhang, B. Qiang, Yan Shen

{"title":"Identification of novel protein-coding genes from human fetal hippocampus ESTs database","authors":"Keyue Ding, Xiaojing Sun, Min Zhou, Jing Cai, Tianjing Cai, Yan Zhang, Tongbin Zhu, Zhengguo Zhang, B. Qiang, Yan Shen","doi":"10.1002/NRC.10042","DOIUrl":"https://doi.org/10.1002/NRC.10042","url":null,"abstract":"We presented a procedure to identify novel protein-coding genes from human fetal hippocampus ESTs database generated in our previous study. The protocol is involved in searching the GenBank non-redundant (nr) protein database with ESTs coding regions query. We used ESTScan program to detect and reconstruct coding regions in the ESTs sequence data. With almost 10,000 raw ESTs analyzed, 4,061 coding regions were extracted. The corresponding amino acid sequences were then searched against the GenBank nr-protein databases; all the similarity hits were parsed in a homology analysis workbench. 22 ESTs were identified as the “seed” ESTs, which might be the fragment of the novel protein-coding genes. Indeed, further analysis of these ESTs in full-length cDNA cloning in silico, as well as the mapping information in UCSC and the added experimental proof, verified the effectiveness of the strategy. Taking advantage of rare ESTs resources, and the effective strategy, we would explore more novel genes in the human genome.\u0000 \u0000 \u0000 \u0000 [The ESTs sequence data described in this paper have been submitted to the GenBank data library (http://www.ncbi.nlm.nih.gov/dbEST) under accession nos. BM174886-958, BM259607-9; and the full length cDNA sequence can be obtained in http://www.chgb.org.cn/hippocampus.htm]","PeriodicalId":19198,"journal":{"name":"Neuroscience Research Communications","volume":"72 1","pages":"93-99"},"PeriodicalIF":0.0,"publicationDate":"2002-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87270389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Tissue xanthine oxidase activity and nitric oxide levels after spinal cord ischemia/reperfusion injury in rabbits: comparison of caffeic acid phenethyl ester (CAPE) and methylprednisolone 兔脊髓缺血/再灌注损伤后组织黄嘌呤氧化酶活性和一氧化氮水平:咖啡酸苯乙酯(CAPE)和甲基强的松龙的比较

Neuroscience Research Communications Pub Date : 2002-09-01 DOI: 10.1002/NRC.10044

Ş. Şahin, S. Sogut, H. Ozyurt, E. Uz, A. Ilhan, O. Akyol

{"title":"Tissue xanthine oxidase activity and nitric oxide levels after spinal cord ischemia/reperfusion injury in rabbits: comparison of caffeic acid phenethyl ester (CAPE) and methylprednisolone","authors":"Ş. Şahin, S. Sogut, H. Ozyurt, E. Uz, A. Ilhan, O. Akyol","doi":"10.1002/NRC.10044","DOIUrl":"https://doi.org/10.1002/NRC.10044","url":null,"abstract":"Caffeic acid phenethyl ester exhibits antioxidant properties. This experimental study was designed to determine the effect of CAPE on nitric NO level and XO activity after reperfusion injury of spinal cord. New Zealand white rabbits were undergone aortic occlusion. CAPE, methylprednisolone or saline were injected intraperitoneally before surgical intervention. Animals were subjected to 21 min of crossclamp time. At the end of occlusion time, the clamps were removed and restoration of the blood flow was verified visually. Animals in sham group underwent a surgical procedure similar to the other groups but the aorta was not occluded. Spinal cord specimens were obtained to determine the tissue levels of NO and XO activity. \u0000 \u0000 \u0000 \u0000Both XO activity and NO level in ischemia group were significantly higher than those of sham group (p = 0.0005 and 0.0003, respectively). MP and CAPE had no effect on XO activity after reperfusion. These two agents decreased NO levels nearly to that of sham group after reperfusion. There were no differences between MP and CAPE on reducing NO level. These results suggest that rabbits with ischemia-reperfusion injury in spinal cord have increased cord concentrations of nitrite and nitrate that are indicative of endogenous overproduction of NO. CAPE may be regarded as an agent that protects spinal cord from ischemia-reperfusion injury.","PeriodicalId":19198,"journal":{"name":"Neuroscience Research Communications","volume":"42 1","pages":"111-121"},"PeriodicalIF":0.0,"publicationDate":"2002-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85114301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21