{"title":"3‐5‐3′‐L‐三碘甲状腺原氨酸对成年大鼠脑皮质突触体Ca2+/Mg2+‐atp酶活性的刺激","authors":"Nilkanta Chakrabarti, A. Ray","doi":"10.1002/NRC.10052","DOIUrl":null,"url":null,"abstract":"Stimulation of the calcium-stimulated and magnesium-dependent adenosine triphosphatase (Ca2+/Mg2+-ATPase) activity by 3-5-3′-L-triiodothyronine (T3) in synaptosomes prepared from adult rat cerebral cortex was demonstrated both in vitro and in vivo. T3 stimulated the Ca2+/Mg2+-ATPase activity in time dependent and dose dependent manner to depolarization-induced synaptosomes suspended in Ca2+-loaded choline-chloride buffer, represented involvement of the recovery phase of the changes in intrasynaptosomal Ca2+ level in this activation. T3 (100nM) also stimulated the enzyme activity in nondepolarized intact synaptosomes suspended in choline-chloride buffer. T3 had direct and dose-dependent stimulatory effect on the Ca2+/Mg2+-ATPase activity of the lysed synaptosomal suspension. The effect of T3 on the lysed synaptosomes was higher than that found on the nondepolarized intact synaptosomes. The condition probably represents the direct and/or indirect interaction of T3 with the enzyme through plasmamembrane related phenomenon that needs further investigation. After T3 treatment, a dose-dependent activation of the enzyme was found in vivo. It appears that T3 plays a role on adult brain neuronal Ca2+-mobilization through stimulation of the Ca2+/Mg2+-ATPase activity in nerve terminals.","PeriodicalId":19198,"journal":{"name":"Neuroscience Research Communications","volume":"32 1","pages":"193-201"},"PeriodicalIF":0.0000,"publicationDate":"2002-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"6","resultStr":"{\"title\":\"Stimulation of Ca2+/Mg2+‐ATPase activity in adult rat cerebrocortical synaptosomes by 3‐5‐3′‐L‐triiodothyronine\",\"authors\":\"Nilkanta Chakrabarti, A. Ray\",\"doi\":\"10.1002/NRC.10052\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Stimulation of the calcium-stimulated and magnesium-dependent adenosine triphosphatase (Ca2+/Mg2+-ATPase) activity by 3-5-3′-L-triiodothyronine (T3) in synaptosomes prepared from adult rat cerebral cortex was demonstrated both in vitro and in vivo. T3 stimulated the Ca2+/Mg2+-ATPase activity in time dependent and dose dependent manner to depolarization-induced synaptosomes suspended in Ca2+-loaded choline-chloride buffer, represented involvement of the recovery phase of the changes in intrasynaptosomal Ca2+ level in this activation. T3 (100nM) also stimulated the enzyme activity in nondepolarized intact synaptosomes suspended in choline-chloride buffer. T3 had direct and dose-dependent stimulatory effect on the Ca2+/Mg2+-ATPase activity of the lysed synaptosomal suspension. The effect of T3 on the lysed synaptosomes was higher than that found on the nondepolarized intact synaptosomes. The condition probably represents the direct and/or indirect interaction of T3 with the enzyme through plasmamembrane related phenomenon that needs further investigation. After T3 treatment, a dose-dependent activation of the enzyme was found in vivo. It appears that T3 plays a role on adult brain neuronal Ca2+-mobilization through stimulation of the Ca2+/Mg2+-ATPase activity in nerve terminals.\",\"PeriodicalId\":19198,\"journal\":{\"name\":\"Neuroscience Research Communications\",\"volume\":\"32 1\",\"pages\":\"193-201\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2002-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"6\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Neuroscience Research Communications\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/NRC.10052\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Neuroscience Research Communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/NRC.10052","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 6
摘要
体外和体内实验证实了3-5-3′- l -三碘甲状腺原氨酸(T3)对成年大鼠大脑皮层突触体钙刺激和镁依赖的腺苷三磷酸酶(Ca2+/Mg2+- atp酶)活性的刺激作用。T3以时间依赖和剂量依赖的方式刺激Ca2+/Mg2+- atp酶活性,以去极化诱导的突触体悬浮在Ca2+负载的氯化胆碱缓冲液中,代表了这种激活中突触体内Ca2+水平变化的恢复阶段的参与。T3 (100nM)也刺激悬于氯化胆碱缓冲液中的未去极化完整突触体的酶活性。T3对裂解的突触体悬浮液的Ca2+/Mg2+- atp酶活性有直接和剂量依赖性的刺激作用。T3对裂解的突触体的影响高于未去极化的完整突触体。这种情况可能是T3通过质膜与酶直接或间接相互作用的结果,需要进一步研究。在T3治疗后,体内发现了一种剂量依赖性的酶激活。似乎T3通过刺激神经末梢Ca2+/Mg2+- atp酶活性,在成人脑神经细胞Ca2+动员中起作用。
Stimulation of Ca2+/Mg2+‐ATPase activity in adult rat cerebrocortical synaptosomes by 3‐5‐3′‐L‐triiodothyronine
Stimulation of the calcium-stimulated and magnesium-dependent adenosine triphosphatase (Ca2+/Mg2+-ATPase) activity by 3-5-3′-L-triiodothyronine (T3) in synaptosomes prepared from adult rat cerebral cortex was demonstrated both in vitro and in vivo. T3 stimulated the Ca2+/Mg2+-ATPase activity in time dependent and dose dependent manner to depolarization-induced synaptosomes suspended in Ca2+-loaded choline-chloride buffer, represented involvement of the recovery phase of the changes in intrasynaptosomal Ca2+ level in this activation. T3 (100nM) also stimulated the enzyme activity in nondepolarized intact synaptosomes suspended in choline-chloride buffer. T3 had direct and dose-dependent stimulatory effect on the Ca2+/Mg2+-ATPase activity of the lysed synaptosomal suspension. The effect of T3 on the lysed synaptosomes was higher than that found on the nondepolarized intact synaptosomes. The condition probably represents the direct and/or indirect interaction of T3 with the enzyme through plasmamembrane related phenomenon that needs further investigation. After T3 treatment, a dose-dependent activation of the enzyme was found in vivo. It appears that T3 plays a role on adult brain neuronal Ca2+-mobilization through stimulation of the Ca2+/Mg2+-ATPase activity in nerve terminals.