NIgerian Journal of Neuroscience最新文献_第2页

In Silico Protein-Ligand Interaction Study of Selected Phytochemicals Against Monoamine Oxidase-B 针对单胺氧化酶 B 的精选植物化学物质的蛋白质配体相互作用硅学研究

NIgerian Journal of Neuroscience Pub Date : 2023-06-30 DOI: 10.47081/njn2023.14.2/004

Patrick Abolarin, Nathaniel Amedu, N. Atasie, B. Owoyele

{"title":"In Silico Protein-Ligand Interaction Study of Selected Phytochemicals Against Monoamine Oxidase-B","authors":"Patrick Abolarin, Nathaniel Amedu, N. Atasie, B. Owoyele","doi":"10.47081/njn2023.14.2/004","DOIUrl":"https://doi.org/10.47081/njn2023.14.2/004","url":null,"abstract":"Parkinson’s disease (PD) is a progressive neurodegenerative disease associated with the death of dopaminergic neurons in the substantia nigra pars compacta. Monoamine oxidase B (MAO-B) inhibitors lessen the degree of PD but still present various side effects. Natural products such as morin, piceatannol, resveratrol, and gallic acid are vital phytochemicals from plants with numerous health benefits. This in silico study aimed to identify potent natural MAO-B inhibitors that could serve as better alternatives to the known MAO-B inhibitors (rasagiline and selegiline). The crystal structure of MAO-B (PDB ID: 2C65) was retrieved from the Protein Data Bank (RSCB) and prepared for molecular docking via Discovery Studio 2020 software. Molecular docking between MAO-B and morin, piceatannol, resveratrol, gallic acid, rasagiline, and selegiline utilised the PyRx software. The Discovery Studio 2020 software was used for visualization. The SwissADME server was used to study the physiochemical properties (Lipinski rule of five), pharma-cokinetic parameters, and absorption, distribution, metabolism, elimination, and toxicity (ADMET) profiles of morin. Comparing results with binding affinity indicated that morin (-10.0 kcal/mol) has a superior potency against 2C65 than piceatannol (-9.0 kcal/mol), resveratrol (-807 kcal/mol), dopamine (-6.4 kcal/mol), gallic acid (-6.3 kcal/mol), rasagiline (-8.0 kcal/mol), and selegiline (-7.4 kcal/mol). Drug candidates comply with all five of Lipinski’s drug-likeness rules with appropriate ADMET properties. Overall, the molecular docking results suggest that morin may be considered a suitable therapeutic candidate for PD treatment.","PeriodicalId":19166,"journal":{"name":"NIgerian Journal of Neuroscience","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139367395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carica papaya Fruit Extract Protects the Cerebellum of Wistar Rats Against Acrylamide-Induced Oxidative Stress 木瓜果实提取物可保护 Wistar 大鼠的小脑免受丙烯酰胺诱导的氧化应激影响

NIgerian Journal of Neuroscience Pub Date : 2023-06-30 DOI: 10.47081/njn2023.14.2/002

Leonard Okah, I. Imosemi

{"title":"Carica papaya Fruit Extract Protects the Cerebellum of Wistar Rats Against Acrylamide-Induced Oxidative Stress","authors":"Leonard Okah, I. Imosemi","doi":"10.47081/njn2023.14.2/002","DOIUrl":"https://doi.org/10.47081/njn2023.14.2/002","url":null,"abstract":"Acrylamide is a water-soluble vinyl monomer produced industrially as an agent for the production of plastics and cosmetics, in waste water management as a flocculation agent, and naturally in fried, baked, and roasted foods, especially when heated beyond 120 °C. Acrylamide (AC) is associated with severe neurotoxic complications through the initiation of oxidative stress. Hence the use of Carica papaya (CP), a natural antioxidant, in protecting the cerebellum of Wistar rats against acrylamide-induced oxidative stress. Forty adult female Wistar rats (180-200 g) were divided into four groups (n=10): Control (1 mL distilled water), AC (25 mg/kg), CP (300 mg/kg), and CP+AC (300 mg/kg+25 mg/kg). All treatments were administered orally for 21 days. Thereafter, the rats were weighed, and neurobehavioural tests were done. The rats were sacrificed, while their cerebella were dissected out and preserved for oxidative stress, antioxidant markers, and histological and immunohistochemical studies. Data were analysed using analysis of variance at p<0.05. There was decreased body weight, locomotor activities, and forelimb grip strength in the AC-treated group compared with the control and other treated groups. Increased lipid peroxidation and nitric oxide levels, decreased glutathione (GSH) levels, and catalase, superoxide dismutase, and glutathione peroxidase activities in the AC-treated groups compared with the control and other treated groups. Histologically, degeneration, pyknosis, shrinking and loss of Purkinje neurons, reactive astrogliosis, and apoptosis occurred in the AC-treated group compared with the control and other treated groups. In conclusion, acrylamide induced oxidative stress in the rat cerebellum, and administration of CP fruit extract offered protection from its neurotoxic effects.","PeriodicalId":19166,"journal":{"name":"NIgerian Journal of Neuroscience","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139367363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-depressant Potentials of Some Bioactive Components of Basella alba Leaves in Chronic Unpredictable Stress Rat Model 白花玄参叶中一些生物活性成分在慢性不可预测压力大鼠模型中的抗抑郁潜能

NIgerian Journal of Neuroscience Pub Date : 2023-06-30 DOI: 10.47081/njn2023.14.2/003

HidaayahO Jimoh-Abdulghaffaar, Emmanuel Areola, Olanrewaju Jimoh, Ayobami Sodimu, Victor Adebosin, Azeez Bakare, Deborah Ipinmoroti, Oluwafisolami Olagbemi, Sarah Yousuph, Jessica Egwa, L. Ojulari

{"title":"Anti-depressant Potentials of Some Bioactive Components of Basella alba Leaves in Chronic Unpredictable Stress Rat Model","authors":"HidaayahO Jimoh-Abdulghaffaar, Emmanuel Areola, Olanrewaju Jimoh, Ayobami Sodimu, Victor Adebosin, Azeez Bakare, Deborah Ipinmoroti, Oluwafisolami Olagbemi, Sarah Yousuph, Jessica Egwa, L. Ojulari","doi":"10.47081/njn2023.14.2/003","DOIUrl":"https://doi.org/10.47081/njn2023.14.2/003","url":null,"abstract":"Bioactive components of Basella alba leaves are responsible for their antidepressant-like activity. However, the component with the greatest anti-depressant activity is unknown. This study investigated the antidepressant-like activities of the bioactive components (phenols, flavonoids, and glycosides) in Basella alba leaves. Forty-two male Wistar rats weighing 50–200 g were randomly divided into six groups (n=7). All the groups, except for the control, were subjected to chronic unpredictable stress (CUS) for five weeks. The rats in the CUS groups were treated with normal saline (1 mL/kg), escitalopram (5 mg/kg), and each of the phenol-, flavonoid-, and glycoside-rich Basella alba extracts (200 mg/kg) orally for twenty-one days. The tail suspension, sucrose preference, light-dark box, and hole-board tests were carried out before and after the induction of depression. In the CUS groups, reduced mobility time on tail suspension, increased percentage sucrose consumption, frequency of head dips on the hole board, and line-cross frequency in the light-dark box were observed. The latency on the hole board was reduced with Basella alba components, while there was a significant (p<0.05) decrease in serum brain-derived neurotrophic factor and an increase in serum IL-6 in the CUS animals not treated with extracts. The phenol-rich Basella alba extract showed the most potent antidepressant-like activity, followed by the flavonoid-rich extract. The bioactive components of Basella alba, particularly phenols, were effective in ameliorating the depressive features of CUS and should be further studied for use as an adjunct or stand-alone antidepressant.","PeriodicalId":19166,"journal":{"name":"NIgerian Journal of Neuroscience","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139367192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tramadol and Coffee Synergy Triggered Oxidative Stress, Altered Cerebellar Histomorphology and Behaviour in Rats 曲马多和咖啡协同作用引发氧化应激，改变小脑组织形态和行为

NIgerian Journal of Neuroscience Pub Date : 2023-04-19 DOI: 10.47081/njn2023.14.1/004

Samuel Umanah, I. Umoh, Aquaisua Aquaisua

{"title":"Tramadol and Coffee Synergy Triggered Oxidative Stress, Altered Cerebellar Histomorphology and Behaviour in Rats","authors":"Samuel Umanah, I. Umoh, Aquaisua Aquaisua","doi":"10.47081/njn2023.14.1/004","DOIUrl":"https://doi.org/10.47081/njn2023.14.1/004","url":null,"abstract":"Tramadol intoxication with other substances has recently raised neurological concerns especially among youths in the society. The effects of tramadol and coffee on the cerebellum of Wistar rats were studied. Thirty adult Wistar rats (average weight of 180 g) were randomly assigned into six groups (n=6) as follows: Control, coffee (72.46 mg/kg), coffee (108.69 mg/kg), tramadol (2.86 mg/kg), tramadol (5.71 mg/kg) and coffee (72.46 mg/kg) + tramadol (2.86 mg/kg). The administrations were orally, and done once daily for twenty-eight consecutive days. On day 29, spontane-ous alternation in the T-maze was carried out, and the rats anaesthetized with ketamine hydrochloride (50 mg/kg intraperitoneally). Their blood were assayed for antioxidant, while the 10 % buffered formalin perfused brains were routinely processed with haematoxylin and eosin technique. Behavioural study showed significantly (p<0.05) increased spontaneous alternation in coffee (108.69 mg/kg), tramadol (5.71 mg/kg), and coffee (72.46 mg/kg) + tramadol (2.86 mg/kg) groups compared to the control. Catalase, superoxide dismutase and reduced glutathione levels were not different (p>0.05), while the malondialdehyde level was significantly (p<0.05) higher in the coffee (72.46 mg/kg) + tramadol (2.86 mg/kg) group compared to the control. Histological observations showed cerebellar cytoarchitectural alterations, including atrophy of the Purkinje cells in the coffee (108.69 mg/kg), tramadol (5.71 mg/kg), and coffee (72.46 mg/kg) + tramadol (2.86 mg/kg) groups compared to the control. In conclusion, co-administration of tramadol and coffee triggered free radicals, damaged the cerebellar tissues and caused a more deleterious effect on memory function than individual doses.","PeriodicalId":19166,"journal":{"name":"NIgerian Journal of Neuroscience","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77879482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Cocos nucifera L. Water on Scopolamine-Induced Memory Impairment in the Wistar Rat 古柯叶水对东莨菪碱致Wistar大鼠记忆损伤的影响

NIgerian Journal of Neuroscience Pub Date : 2023-04-01 DOI: 10.47081/njn2023.14.1/003

Busayo Oladun, A. Oyerinde, Ayodele Oyadeyi, B. Ayinde, A. Onasanwo

{"title":"Effect of Cocos nucifera L. Water on Scopolamine-Induced Memory Impairment in the Wistar Rat","authors":"Busayo Oladun, A. Oyerinde, Ayodele Oyadeyi, B. Ayinde, A. Onasanwo","doi":"10.47081/njn2023.14.1/003","DOIUrl":"https://doi.org/10.47081/njn2023.14.1/003","url":null,"abstract":"Memory loss is an important Alzheimer's disease indicator (AD) linked with hippocampal damage. Scopolamine (SCO) antagonizes muscarinic acetylcholine receptor (mAChR) whose effect is similar to AD symptoms. Cocos nucifera L. (coconut) water is a crucial biological fluid containing nutritional components, and consumed for its health benefits. This study investigated the effect of C. nucifera water on memory impairment caused by SCO in rats. Thirty-two male Wistar rats were divided into four groups (n=8): control (normal saline); SCO (1 mg/kg/i.p.), C. nucifera water (0.4 mL/20 g) + SCO (1 mg/kg/i.p.), and donepezil (5 mg/kg/i.p.)+ SCO (1 mg/kg/i.p.). The levels of acetylcholinesterase (AChE), brain-derived neurotrophic factor (BDNF), interleukin-6 (IL-6), tumour necrosis factor (TNF-α), and cyclo-oxygenase-2 (Cox-2) were measured. Hippocampal histology was done. Utilizing GraphPad Prism for statistical analy-sis, pre-treatment with C. nucifera water and donepezil displayed higher (p<0.01) spontaneous alternation in the Y-maze, and decreased escape latency, increased entries and time spent in the target quadrant (p<0.01) of the Morris water maze compared to the SCO group. These pre-treatment also inhibited AChE activity (p<0.001), and reduced expressions of IL-6, TNF-α and Cox-2 (p<0.001). However, there were no marked differences (p>0.05) in BDNF across the groups. Histology of SCO-only group revealed degenerated neurons, whereas the pre-treated groups showed regenerated neurons with less necrotic cells. The administration of SCO to the rats resulted in some form of memory impairment, and C. nucifera water showed promise for reversing this deficits","PeriodicalId":19166,"journal":{"name":"NIgerian Journal of Neuroscience","volume":"57 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84090847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Acute Aerobic Exercise on Working Memory Among Female Students of Bingham University, Nigeria 急性有氧运动对尼日利亚宾汉姆大学女学生工作记忆的影响

NIgerian Journal of Neuroscience Pub Date : 2023-04-01 DOI: 10.47081/njn2023.14.1/002

Susan Ogbe, A. Danborno, Mercy Oluwayomi, Mahan Mallo, Sheba Ishaku

{"title":"The Effect of Acute Aerobic Exercise on Working Memory Among Female Students of Bingham University, Nigeria","authors":"Susan Ogbe, A. Danborno, Mercy Oluwayomi, Mahan Mallo, Sheba Ishaku","doi":"10.47081/njn2023.14.1/002","DOIUrl":"https://doi.org/10.47081/njn2023.14.1/002","url":null,"abstract":"Researches have showed that participation in a physical activity is associated with increased performance across a variety of tasks involving attention, cognition, and memory. The present study aimed at investigating the effects of aerobic exercise on working memory in non-athletic female students of Bingham University. A total of 53 participants who completed the physical activity readiness questionnaire (PAR-Q) were considered for this study. The study was conducted in three days, with day 1 being the baseline testing. Participants completed an N-back working memory task before the start of aerobic exercise. They were then required to perform an aerobic exercise on the treadmill for 10 min; and after which, were made to perform the N-back working memory task again. The same procedure was carried out on days 2 and 3. All data were analysed using analysis of variance at p<0.05. A significant (p<0.001) increase in total false alarm was observed in subjects after exercise and on days 2 and 3 compared to the first day before exercise. There was significant decrease in reaction time after the exercise, and on days 2 and 3 of the study as compared to the first day at p<0.001. This study has shown that acute aerobic exercise improved working memory in the female students’ population","PeriodicalId":19166,"journal":{"name":"NIgerian Journal of Neuroscience","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80261513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vanadium Impairs Neuronal Cytoarchitecture of the Hippocampal Trisyn-aptic Loop in the African Giant Rat (Cricetomys gambianus, Waterhouse) 钒对非洲巨鼠海马trisins -aptic环神经元细胞结构的影响

NIgerian Journal of Neuroscience Pub Date : 2023-02-10 DOI: 10.47081/njn2023.14.1/001

O. Mustapha, Micheal Awala-Ajakaiye, Boluwatife Agbalu, S. Bello, M. Olude, James Olopade

{"title":"Vanadium Impairs Neuronal Cytoarchitecture of the Hippocampal Trisyn-aptic Loop in the African Giant Rat (Cricetomys gambianus, Waterhouse)","authors":"O. Mustapha, Micheal Awala-Ajakaiye, Boluwatife Agbalu, S. Bello, M. Olude, James Olopade","doi":"10.47081/njn2023.14.1/001","DOIUrl":"https://doi.org/10.47081/njn2023.14.1/001","url":null,"abstract":"Environmental pollution with vanadium may pose neurotoxicity threats to known unique neural attributes (cognition and olfaction) of the African giant rat (AGR). This rodent lives within the same ecological zones as the human populace. Thus, experimental investigations on the effect of vanadium on this rodent may mirror latent epidemiological scenarios in the human populace. This work was designed to evaluate the neurotoxic effect of vanadium on the hippocampal neuronal infrastructure and circuitry and provide cellular correlates to its significant pathologies in the AGR. Twelve adult male AGRs were assigned into two groups (n = 6/group; vanadium and control). They were dosed daily with 3 mg/kg body sodium metavanadate and sterile water for 14 days, respectively, and harvested brains were processed for histology. Using Nissl and Golgi staining techniques with stereological analysis, we demonstrated the effect of vanadium on AGR neuronal architecture of the hippocampal trisynaptic loop as a probable cellular mechanism of vanadium-induced memory deficits. Specifically, the most significant pathologies were seen in the dentate gyrus and CA3, with significantly decreased neuronal density disrupted cytoarchitecture and loss of dendritic arborisations and axonal extensions. At the same time, hippocampal subfields CA2 and CA4 showed region-specific resistance to vanadium-induced neurotoxicity. Furthermore, the selective vulnerability to vanadium-induced neurotoxicity is adduced. This work has demonstrated the neurotoxic effect of vanadium on the hippocampal subfields and circuitry in the AGR and highlighted its probable impact on their translational purposes. This rodent may be a suitable model for evaluating memory pathologies in neurotoxicological studies","PeriodicalId":19166,"journal":{"name":"NIgerian Journal of Neuroscience","volume":"74 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90363993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Astrocytic Response in the Striatum and Corpus Callosum of the Brain of Adult Wistar Rats with Global Ischaemia 全脑缺血成年Wistar大鼠脑纹状体和胼胝体的星形细胞反应

NIgerian Journal of Neuroscience Pub Date : 2022-12-31 DOI: 10.47081/njn2022.13.4/005

M. Shokunbi, F. Olopade, O. Femi-Akinlosotu, Olasiji Oladeji

{"title":"Astrocytic Response in the Striatum and Corpus Callosum of the Brain of Adult Wistar Rats with Global Ischaemia","authors":"M. Shokunbi, F. Olopade, O. Femi-Akinlosotu, Olasiji Oladeji","doi":"10.47081/njn2022.13.4/005","DOIUrl":"https://doi.org/10.47081/njn2022.13.4/005","url":null,"abstract":"Ischaemic stroke occurs when the blood supply to a part of the brain is suddenly interrupted. Astrocytes are known to maintain ionic homeostasis, scavenge free radicals and support neurogenesis. This study examined the astrocytic responses in the corpus callosum and striatum to global ischaemia with functional deficits in adult male rats. The common carotid artery (CCA) in adult rats was occluded temporarily (30 min) or permanently and then kept for 24 h or three days, while a sham operation was done for the control (n=10 per group). Neurobehavioural testing for motor deficits (square grid and pole tests) were carried out before sacrifice. The rats’ brains were stained with haematoxylin and eosin, and immunohistochemically with anti-glial fibrillary acidic protein for astrocytes. Quantitative data from behavioural tests were compared using analysis of variance, and significance was set at 0.05. The rats with permanent CCA occlusion had increased latency to turn and return to the base in the pole test, and reduced latency to fall in the square grid test compared to the temporary occlusion and control groups. Sections of the corpus callosum and striatum had increased proliferation and hypertrophy of the astrocytes in the permanent CCA occlusion, which was less obvious in the temporary occlusion group compared to the control. Global ischaemia caused neuronal degeneration and reactive astrogliosis in the corpus callosum and striatum, which were more pronounced in the group with permanent arterial occlusion than those that underwent reperfusion. Early reperfusion is crucial for structural and functional recovery following brain ischaemia in stroke management, and astrocytes play important roles in both the pathogenesis and recovery process.","PeriodicalId":19166,"journal":{"name":"NIgerian Journal of Neuroscience","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74844409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mucuna pruriens Constituted Diet Ameliorates Manganese-Induced Behavioural Deficit and Histological Alterations in the Brains of Rats 含粘液成分的饮食改善锰诱导的大鼠脑行为缺陷和组织学改变

NIgerian Journal of Neuroscience Pub Date : 2022-12-31 DOI: 10.47081/njn2022.13.4/003

Olayi Ogamode, Micheal Obu, O. Ijomone

{"title":"Mucuna pruriens Constituted Diet Ameliorates Manganese-Induced Behavioural Deficit and Histological Alterations in the Brains of Rats","authors":"Olayi Ogamode, Micheal Obu, O. Ijomone","doi":"10.47081/njn2022.13.4/003","DOIUrl":"https://doi.org/10.47081/njn2022.13.4/003","url":null,"abstract":"Overexposure to manganese (Mn) has been clearly established to trigger neurotoxicity that may result in Parkinson-like symptoms. Mucuna pruriens have been reported to possess constituents that could be neuroprotective. The present study evaluated the effect of M. pruriens constituted diet in Mn-induced memory and motor deficits, as well as on histological changes in the brain. Twenty-four adult Wistar rats were randomly assigned into four groups of six rats. Three groups of animals were subjected to Mn-administration and on standard feed, 10% or 20% M. pruriens constituted feeds for a 5-week duration. The remaining group served as control and received standard feed only. Following experimental treatments, rats were subjected to the Y-maze test and the open-field test. Furthermore, brains were excised and evaluated with routine haematoxylin and eosin histological protocol. Result of spontaneous alternation in the Y-maze test showed that Mn caused decreased memory performance and decreasing motor and explorative activities, but co-treatment with M. pruriens mitigated behavioural impairments. Additionally, Mn-administration resulted in noticeable neurodegenerative features in the cerebellum, hippocampus and striatum. However, concurrent use of M. pruriens diet slightly attenuated histological alterations. In conclusion, the present study suggests that treatment with M. pruriens may provide ameliorative benefits against Mn-induced neurotoxicity","PeriodicalId":19166,"journal":{"name":"NIgerian Journal of Neuroscience","volume":"22 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91489543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of in-vivo Vanadium-Induced Neurotoxicity and Withdrawal on Three Consecutive Generations of Mice Brains: A Neurobehavioural and Histo-Immunohistochemical Study 体内钒诱导的神经毒性和戒断对连续三代小鼠大脑的影响:神经行为和组织免疫组织化学研究

NIgerian Journal of Neuroscience Pub Date : 2022-12-31 DOI: 10.47081/njn2021.12.4/002

A. Lijoka, O. Igado, F. Olopade, James Olopade

{"title":"Effects of in-vivo Vanadium-Induced Neurotoxicity and Withdrawal on Three Consecutive Generations of Mice Brains: A Neurobehavioural and Histo-Immunohistochemical Study","authors":"A. Lijoka, O. Igado, F. Olopade, James Olopade","doi":"10.47081/njn2021.12.4/002","DOIUrl":"https://doi.org/10.47081/njn2021.12.4/002","url":null,"abstract":"Heavy metals exposure causes multi-systemic pathologies in biological systems. Due to their genotoxic effects, some of these anomalies have been reported to transcend to unexposed generations. Vanadi-um, a transition metal, crosses the blood brain barrier, causing neuroinflammatory and demyelinating lesions with neurobehavioural inadequacies in exposed subjects. There are however scarce scientific information on vanadium neurotoxicity over generations of exposed subjects. This study explored the effects of vanadium exposure on the body weight, neurobehavioural (open field and negative geotaxis tests) and neurohistological changes (Purkinje cell, astroglial and myelin histoarchitecture) in three consecutive generations – two exposed generations (G1 and G2) and one withdrawal generation (G3), of mice. Neonatal pups of BALB/c mice in G1 and G2, were treated with sodium metavanadate every 48 h, first via lactation from post natal day (PND) 1-14, and later intraperitoneally (PND 15-21) for males; PND 15-45 for female pups till they were mated with unexposed males. The G3 pups were not treated with sodium metavanadate. The vanadium treated pups had irregular body weight gain. In all the studied generations (G1-3), there were reduced locomotor and exploratory activities, and diminished motor and vestibular function compared to the controls. Furthermore, there was multilayering and pyknosis of the cerebellar Purkinje cells, moderate to marked astrogliosis and generalized demyelination in the corpus callosum, hippocampus (CA1-4 and the dentate gyrus), cerebral cortex, thalamus and cerebellum. This study revealed that effects of vanadium exposure including neurobehavioural anomalies, astrogliosis, demyelination and cerebellar Purkinje cell pathology can persist across multiple generations, including generation not initially exposed","PeriodicalId":19166,"journal":{"name":"NIgerian Journal of Neuroscience","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79618902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0