Neuroimmunology and Neuroinflammation最新文献_第8页

Interleukin-1β-induced inflammatory signaling in C20 human microglial cells 白细胞介素-1β诱导的C20人小胶质细胞炎症信号

Neuroimmunology and Neuroinflammation Pub Date : 2018-12-26 DOI: 10.20517/2347-8659.2018.60

R. Davis, D. Buck, K. McCracken, G. W. Cox, Subhas Das

{"title":"Interleukin-1β-induced inflammatory signaling in C20 human microglial cells","authors":"R. Davis, D. Buck, K. McCracken, G. W. Cox, Subhas Das","doi":"10.20517/2347-8659.2018.60","DOIUrl":"https://doi.org/10.20517/2347-8659.2018.60","url":null,"abstract":"Aim: Increased inflammatory signaling in microglia is implicated in the pathogenesis of neurodegenerative diseases, trauma, psychiatric disorders, and anxiety/depression. Understanding inflammatory signaling in microglia is critical for advancing treatment options. Studying rodent-derived microglia has yielded substantial information, yet, much remains to better understand inflammatory signaling in human microglia. Hence, there is great interest in developing immortalized human microglial cell lines. The C20 human microglial cell line was recently developed and our primary objective was to advance our knowledge of inflammatory signaling in these cells. Methods: Expression of the microglia specific marker transmembrane protein 119 (TMEM119) was assessed by western blot analysis. Lipopolysaccharide (LPS)and interleukin-1β (IL-1β)-induced cytokine/chemokine expression was determined by ELISA. Phosphorylation of inhibitory kappa B alpha (IκBα), nuclear factor (NF)-κB p65, and p38 mitogen-activated protein kinase (p38 MAPK) was measured by western blot analysis. Results: TMEM119 was expressed in unstimulated C20 cells, and to a greater extent in IL-1β-stimulated cells. IL-1β significantly induced IL-6, monocyte chemoattractant protein-1/CCL2, and interferon-γ inducible protein 10/CXCL10 expression. LPS induced CCL2 expression, but not IL-6 or CXCL10 expression. IL-1β induced inflammatory signaling as indicated by increased phosphorylation of IκBα, NF-κB p65 and p38 MAPK. Conclusion: We provide the first evidence that C20 microglia express TMEM119. This is the initial report of IL-1βinduced activation of IκBα, NF-κB p65, and p38 MAPK and subsequent CXCL10, CCL2 and IL-6 secretion in C20 cells. These findings advance our understanding of inflammatory signaling in C20 cells and support the value of this cell line as a research tool.","PeriodicalId":19129,"journal":{"name":"Neuroimmunology and Neuroinflammation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44224249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Cerebral autosomal dominant arteriopathy with subcortical infarcts and leucoencephalopathy vs. multiple sclerosis. Either one or sometimes both? 伴有皮质下梗死和白质脑病的常染色体显性遗传性脑动脉病与多发性硬化症。要么一个，有时两个？

Neuroimmunology and Neuroinflammation Pub Date : 2018-12-14 DOI: 10.20517/2347-8659.2018.50

G. Paraskevas, V. Constantinides, E. Kapaki

引用次数: 4

Paraneoplastic limbic encephalitis associated with testicular mixed germ cell tumor 伴睾丸混合生殖细胞瘤的肿瘤旁边缘脑炎

Neuroimmunology and Neuroinflammation Pub Date : 2018-12-10 DOI: 10.20517/2347-8659.2018.55

D. Almedallah, Gada Alsaffar, Ghadeer Al-Shabeeb, A. Baarmah, E. Nassim

引用次数: 1

Immunotherapy and checkpoint inhibitors for gliomas 神经胶质瘤的免疫治疗和检查点抑制剂

Neuroimmunology and Neuroinflammation Pub Date : 2018-11-15 DOI: 10.20517/2347-8659.2018.46

Clairice Pearce, M. Chrostek, Emily G. Fellows, Nikolas G. Toman, Sarah K. Tran, A. Crane, W. Low

{"title":"Immunotherapy and checkpoint inhibitors for gliomas","authors":"Clairice Pearce, M. Chrostek, Emily G. Fellows, Nikolas G. Toman, Sarah K. Tran, A. Crane, W. Low","doi":"10.20517/2347-8659.2018.46","DOIUrl":"https://doi.org/10.20517/2347-8659.2018.46","url":null,"abstract":"Glioma treatments are faced with challenges, including the inability to fully eliminate cancer stem cells, the immunosuppressive tumor microenvironment, and the blood brain barrier. Although progress has been made with surgical, radiation, and chemotherapies, prognosis for patients remains poor. Rapidly emerging immunotherapies may be able to address the challenges that conventional techniques cannot. Immunotherapies manipulate the patient’s immune system to selectively combat malignancies. Therapies often work to enhance T-cell and natural killer (NK) cell function, which can both eliminate tumor cells and enhance remission. Vaccines encourage in vivo development of anti-tumor T-cells and NK cells, while adoptive transfer techniques focus on engineering immune cells ex vivo before reintroducing them to patients. Vaccine and adoptive transfer therapies have been shown to induce enhanced immune responses in patients but have not always correlated with improved outcomes, likely because of the tumor immunosuppressive microenvironment. Checkpoint inhibitors can impair these tumor immunosuppressive capabilities. Although no one treatment has been able to consistently eliminate gliomas and maintain remission, combinations of vaccines or adoptive transfer techniques in conjunction with immune checkpoint inhibitors offers promise.","PeriodicalId":19129,"journal":{"name":"Neuroimmunology and Neuroinflammation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45990985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Total tumor RNA pulsed dendritic cells plus adoptive transfer of ex-vivo enriched autologous T-lymphocytes in the treatment of children with primary brain tumors 肿瘤总RNA脉冲树突状细胞加体外富集的自体t淋巴细胞过继转移治疗儿童原发性脑肿瘤

Neuroimmunology and Neuroinflammation Pub Date : 2018-10-29 DOI: 10.20517/2347-8659.2018.44

S. Gururangan, E. Sayour, D. Mitchell

引用次数: 5

Correction: In silico design of novel gold-phosphate containing compounds as selective inhibitors of cathepsin B in neuroinflammation 修正:新型含金-磷酸化合物在神经炎症中作为组织蛋白酶B的选择性抑制剂的硅设计

Neuroimmunology and Neuroinflammation Pub Date : 2018-10-22 DOI: 10.20517/2347-8659.2018.61

Alexsey V. Raevsky, M. Sharifi, V. Pinchuk, A. Klegeris

引用次数: 0

CAR-T cell therapy in neuro-oncology: applications and toxicity CAR-T细胞疗法在神经肿瘤学中的应用和毒性

Neuroimmunology and Neuroinflammation Pub Date : 2018-10-18 DOI: 10.20517/2347-8659.2018.51

Akanksha Sharma, Gustavo De Leon, A. Porter, Marie F. Grill, A. Rosenthal, Christine E. Brown, K. Swanson, M. Mrugala

引用次数: 4

It takes two: potential therapies and insights involving microglia and macrophages in glioblastoma 它需要两个方面:胶质母细胞瘤中涉及小胶质细胞和巨噬细胞的潜在疗法和见解

Neuroimmunology and Neuroinflammation Pub Date : 2018-10-18 DOI: 10.20517/2347-8659.2018.47

John Choi, N. Mai, Christopher M. Jackson, Z. Belcaid, M. Lim

{"title":"It takes two: potential therapies and insights involving microglia and macrophages in glioblastoma","authors":"John Choi, N. Mai, Christopher M. Jackson, Z. Belcaid, M. Lim","doi":"10.20517/2347-8659.2018.47","DOIUrl":"https://doi.org/10.20517/2347-8659.2018.47","url":null,"abstract":"Microglia and macrophages, two myeloid cell lineages with different origins, make up the majority of immune cells present in glioblastoma (GBM). However, much of the literature does not distinguish between microglia and macrophages, despite a growing body of evidence that demonstrates key structural and functional differences between the cell types. Furthermore, the current M1/M2 paradigm used to sub-classify microglia and macrophages has proven to be incomplete at best, with the growing amount of in vivo and genomic data incompatible with this dichotomy. Finally, a number of studies have already established that in the setting of the GBM tumor microenvironment, both microglia and macrophages are complicit in tumor progression. This review highlights the differences between microglia and macrophages, particularly in the context of GBM, and discusses at length several potential therapeutic strategies made possible by understanding specific pro-tumor and anti-tumor pathways in these myeloid populations. Ultimately, investigating the differences between microglia and macrophages offers insight into the progression of GBM, its marked resistance to current immunotherapy regimens, and future directions for new treatment modalities.","PeriodicalId":19129,"journal":{"name":"Neuroimmunology and Neuroinflammation","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2018-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48969856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Changes in microglia activity of rat brain induced by Macrovipera lebetina obtusa venom 毒蜥毒素诱导大鼠脑小胶质细胞活性的变化

Neuroimmunology and Neuroinflammation Pub Date : 2018-09-29 DOI: 10.20517/2347-8659.2018.33

A. Darbinyan, M. Antonyan, H. Koshatashyan, Susanna S. Gevorgyan, H. Arestakesyan, Z. Karabekian, N. Ayvazyan, A. Voskanyan

引用次数: 1

Presynaptic mechanisms at prefrontal synapses involved in persistent pain 参与持续疼痛的前额叶突触突触前机制

Neuroimmunology and Neuroinflammation Pub Date : 2018-09-29 DOI: 10.20517/2347-8659.2018.48

H. Toyoda

引用次数: 0