Neurobiology最新文献_第3页

Effect of essential fatty acids on proliferation of two neural tumor lines. 必需脂肪酸对两种神经肿瘤细胞系增殖的影响。

Neurobiology Pub Date : 1975-08-01

E Yavin, Z Yavin, J H Menkes

引用次数: 0

Sialoglycoprotein synthesis in developing rat brain. 发育中的大鼠脑唾液糖蛋白合成。

Neurobiology Pub Date : 1975-08-01

D H van den Eijnden, W van Dijk, P A Roukema

引用次数: 0

Morphological changes of astrocyte-like cells induced by serum beta-lipoprotein in brain cell culture. 血清β -脂蛋白诱导脑细胞培养星形细胞样细胞的形态学改变。

Neurobiology Pub Date : 1975-06-01

H Offner, G Konat, J Clausen

引用次数: 0

Separation of brain-RNA by micro-electrophoresis on agarose-acrylamide gels. 琼脂糖-丙烯酰胺凝胶微电泳分离脑rna。

Neurobiology Pub Date : 1975-06-01

A Cupello, H Hydén

{"title":"Separation of brain-RNA by micro-electrophoresis on agarose-acrylamide gels.","authors":"A Cupello, H Hydén","doi":"","DOIUrl":"","url":null,"abstract":"A simple method is described for RNA fractionation on the microscale (10(-8) to 10(-7) g). The optimal conditions for electrophoretic separation of low molecular weight RNA (4S-16S) on such a scale have been determined running a standard mixture of 4S, 5S and 16S E. coli RNAs on agarose-polyacrylamide gels at three different concentrations of acrylamide. The separations were evaluated for resolution of the different species and for linearity in the relationship between mobility and logarithm of molecular weight. A straight linear relationship and good resolution was obtained on a 3.48 per cent polyacrylamide - 0.7 per cent agarose gel. When a sample of 10(-2) O.D.U.260 of macroextracted cerebral RNA was separated on such a gel, six unusual RNA bands were detected between 5A and 18S. To find out optimal conditions for RNA fractionation in the 16S-28S range a standard mixture of 16S, 18S, 23S and 28S RNA was run on gels of three different acrylamide concentrations. The separations were again evaluated with regard to both the resolution and the linearity of the relationship between the logarithms of the molecular weights and the mobilities of the standard RNA species. A 0.7 per cent agarose - 2.5 per cent polyacrylamide gel appeared to be quite suitable with regard to both. The fractionation of a microsample of RNA macroextracted from whole rat brain is presented. We also present the fractionation of the RNA microextracted from 1 mg of the CA3 region of rat hippocampus.","PeriodicalId":19126,"journal":{"name":"Neurobiology","volume":"5 3","pages":"129-36"},"PeriodicalIF":0.0,"publicationDate":"1975-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12282107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protein bound radioactivity in neuronal and glial fractions following intra-carotid 3H-leucine perfusion. 颈动脉内3h -亮氨酸灌注后神经元和胶质部分蛋白结合放射性的变化。

Neurobiology Pub Date : 1975-06-01

C Blomstrand, A Hamberger, A Sellström, O Steinwall

引用次数: 0

Fractionation of protein carried by axoplasmic transport. II. Comparison in the rat of proteins carried to the optic relay nuclei. 由轴浆运输携带的蛋白质的分离。2大鼠光传递核携带蛋白的比较。

Neurobiology Pub Date : 1975-06-01

L G Siegel, W O McClure

引用次数: 0

The effect of pre- and postnatal undernutrition on the development of the cerebellar cortex in the rat. II. Histochemical observations. 产前和产后营养不良对大鼠小脑皮质发育的影响。2组织化学观察。

Neurobiology Pub Date : 1975-06-01

L Persson, A Sima

引用次数: 0

Measurement of protein in cultures of nervous tissue grown on collagen substrate. 胶原基质上神经组织培养物中蛋白质含量的测定。

Neurobiology Pub Date : 1975-06-01

T H Oh, S U Kim, D D Johnson

引用次数: 0

Disease-inducing activity of different parts of bovine encephalitogenic protein in guinea-pigs. 牛致脑蛋白不同部位在豚鼠体内的致病活性。

Neurobiology Pub Date : 1975-06-01

J Lindh, H Bergstrand

引用次数: 0

Brain proteins in undernourished rats during learning. 营养不良大鼠学习过程中的脑蛋白。

Neurobiology Pub Date : 1975-05-01

H Hydén, P W Lange

{"title":"Brain proteins in undernourished rats during learning.","authors":"H Hydén, P W Lange","doi":"","DOIUrl":"","url":null,"abstract":"The effect of protein calorie undernourishment was studied in the hippocampus, the visual and the sensory-motor cortex of rats, subjected to a reversal learning test, with respect to protein fractions containing the acid proteins S-100 and 14-3-2. These proteins are brain specific and are confined to the nervous system of vertebrates and invertebrates. The 14-3-2 protein is localized in the fractions 4 and 5 counted from the anodal front in an acrylamide electrophoretic separation. Incorporation of 1-14-C-leucine and 3-H-leucine was determined in single and double-labeling experiments. The rats learned to discriminate between dark and light in a reversal and in a final re-reversal test. Extinction rats served as a comparison to trained rats although we stress the comparison trained, undernourished versus trained, fully fed rats. Behaviorally, the undernourished rats showed lower acquisition expressed as number of correct responses per trial block, but a somewhat higher rate of acquisition compared to the fully fed rats. In the untrained rats (undernourished versus fully fed) the following was found: a decreased amount of S100 in the visual cortex; an increased amount of S100 in the sensory-motor cortex. Significant differences existed in the biochemical response between the two groups of rats in the learning test (trained, undernourished versus trained, fully fed rats): decreased relative specific activities of the hippocampal S100, 4 and 5 proteins, and the 4 protein of the visual cortex; but increased relative specific activities of the 4 and 5 proteins of the sensory-motor cortex. Evidence is presented that these protein changes are specific. The changed response of the undernourished rats is interpreted as an adaption of the central nervous system to the stress on the organism induced by the protein calorie deficiency.","PeriodicalId":19126,"journal":{"name":"Neurobiology","volume":"5 2","pages":"84-100"},"PeriodicalIF":0.0,"publicationDate":"1975-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11994012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0