由轴浆运输携带的蛋白质的分离。2大鼠光传递核携带蛋白的比较。

Neurobiology Pub Date : 1975-06-01
L G Siegel, W O McClure
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引用次数: 0

摘要

用眼内注射放射性l -蛋氨酸标记白化大鼠视网膜神经节细胞轴浆快速流动(200 mm/天)中携带的蛋白质。注射后4 1/2小时,用增溶力培养基提取积聚在膝状外侧核(LGN)和上丘(SC)的转运蛋白。超过70%的输送物质只能在含有Triton X-100和十二烷基硫酸钠等洗涤剂的介质中溶解。转运蛋白比活性超过7000 dpm/mg。具有如此高的比活度,可以在高分辨率条件下进行进一步分馏。溶解的蛋白质在聚丙烯酰胺凝胶上的分离,无论有或没有洗涤剂,表明大量(60-100)多肽被运输。使用L-[35S]蛋氨酸和L-[甲基- 3h]蛋氨酸的双标记程序,将转运到LGN的物质与转运到SC的物质进行比较。从视网膜神经节细胞转运到两个主要视神经传递核的多肽数量没有统计学上的显著差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fractionation of protein carried by axoplasmic transport. II. Comparison in the rat of proteins carried to the optic relay nuclei.

Proteins carried in the fast (200 mm/day) axoplasmic flow of retinal ganglion cells in albino rats were labeled with intraocular injections of radioactive L-methionine. Transported proteins which accumulated in the lateral geniculate nuclei (LGN) and superior colliculi (SC) 4 1/2 hours after injection were extracted with media of increasing solubilizing power. Over 70 percent of the transported material is soluble only in media possessing detergents such as Triton X-100 and sodium dodecyl sulfate. Specific activities of the transported proteins exceeded 7000 dpm/mg. With specific activities this high, further fractionation could be carried out under conditions of high resolution. Separations of solubilized proteins on polyacrylamide gels, either with or without detergents, showed that a large number (60-100) of polypeptides are transported. Using a double labeling procedure which employed L-[35S]methionine and L-[methyl-3H]methionine, material transported to the LGN was compared with that transported to the SC. No statistically significant differences were seen in the polypeptides transported by the retinal ganglion cells to the two major optic relay nuclei.

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