Neurobiology最新文献

Incorporation of [3H] leucine into brain stem protein fractions: the effect of a behaviorally active, N-terminal fragment of ACTH in hypophysectomized rats. [3H]亮氨酸掺入脑干蛋白组分:垂体去切大鼠ACTH n端行为活性片段的影响

Neurobiology Pub Date : 1975-12-01

M E Reith, P Schotman, W H Gispen

引用次数: 0

The use of hybrid molecules in a study of the equilibrium between nerve growth factor monomers and dimers. 杂化分子在神经生长因子单体和二聚体之间平衡的研究中的应用

Neurobiology Pub Date : 1975-12-01

J B Moore, E M Shooter

{"title":"The use of hybrid molecules in a study of the equilibrium between nerve growth factor monomers and dimers.","authors":"J B Moore, E M Shooter","doi":"","DOIUrl":"","url":null,"abstract":"The major protein in beta nerve growth factor preparations, beta1NGF, is a dimer in which both peptide chains have COOH-terminal arginine residues. Digestion of beta1NGF with carboxypeptidase B produced a dimer, beta3NGF, in which both chains lack these terminal arginine residues. Exposure of mixtures of beta1 and beta3NGF dimers to 8 M urea to produce monomers, followed by removal of urea to allow recombination, resulted in the formation of the hybrid beta2NGF, comprising one arginine-containing and one arginine-less chain, as well as the parent dimers. The amount of the three dimers formed was close to that expected from random association of monomers. Hybrid beta2NGF was also formed from mixtures of beta1 and beta3NGF where incubated at pH 2.6 to 4.5. The formation of beta2NGF has a half-time of 6 h at pH 4.0 and 4 degrees C. Its rate of formation decreased above pH 4.5, becoming minimal between pH 9.5 and pH 10.5, and increased with increasing temperature. The amount of beta2NGF formed was determined by the lowest pH to which the parent mixture was exposed, irrespective of its prior history. These data suggest that the hybrid is formed by the same mechanism in the absence and presence of the urea step. An approximate value for Kd, the equilibrium dissociation constant of the dimer equilibrium monomer equilibrium was derived. Its value was 3 - 10(-10) M at pH 4.0 and 4 degrees C. The alpha-subunit of 7S NGF decreased the rate of formation of beta2NGF not only at pHs where an alphabeta complex is stable, but also at an acid pH where no complex formation is observed by sedimentation analysis, suggesting that the present methodology offers a more sensitive probe of subunit interactions. In contrast, the gamma subunit and a number of indifferent proteins had little or no effect on the appearance of beta2NGF at the pHs studied.","PeriodicalId":19126,"journal":{"name":"Neurobiology","volume":"5 6","pages":"369-81"},"PeriodicalIF":0.0,"publicationDate":"1975-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11226008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distribution and age-dependent concentration in brain tissue of glycoproteins containing N-acetylgalactosamine. 含n -乙酰半乳糖胺的糖蛋白在脑组织中的分布和年龄依赖性浓度。

Neurobiology Pub Date : 1975-12-01

E G Brunngraber, B D Brown, A Aro

引用次数: 0

Dissociated cultures of newborn mouse brain. I. Metabolism of sulfated lipids and mucopolysaccharides. 新生小鼠大脑的分离培养。1 .硫酸脂质和粘多糖的代谢。

Neurobiology Pub Date : 1975-12-01

U N Wiesmann, K Hofmann, T Burkhart, N Herschkowitz

{"title":"Dissociated cultures of newborn mouse brain. I. Metabolism of sulfated lipids and mucopolysaccharides.","authors":"U N Wiesmann, K Hofmann, T Burkhart, N Herschkowitz","doi":"","DOIUrl":"","url":null,"abstract":"The metabolism of 35SO4-sulfated lipids and mucopolysaccharides was studied in dissociated brain cell cultures from newborn albino mouse brains. The cultures were maintained under an atmosphere of 40% O2 and 5% CO2 in apparent good health up to 30 days. Early morphological examination of the dissociated cells demonstrated an initial partial reaggregation of the cells, which later settled and became confluent bilayered cultures. Cell proliferation measured by DNA and protein determination, morphological differentiation and biochemical differentiation took place in the dissociated brain cell cultures analogous in some respects to the in vivo situation. A timed increase in the synthesis of a myelin precursor, cerebroside 35SO4, was observed after 6 to 8 days in culture (DIC). A peak of cerebroside sulfate was evident at 17 DIC. No stable sulfatide was observed at any time. Protein-bound macromolecular 35SO4-MPS was synthetized and secreted from the cells into the culture medium. Maximal synthesis and secretion occurred at 8 DIC. This culture system proves to be a useful model for studying some aspects of differentiation of brain cells under controlled external conditions.","PeriodicalId":19126,"journal":{"name":"Neurobiology","volume":"5 6","pages":"305-15"},"PeriodicalIF":0.0,"publicationDate":"1975-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"11348614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The preparation and properties of nerve growth factor protein at alkaline pH. 碱性条件下神经生长因子蛋白的制备及性能研究。

Neurobiology Pub Date : 1975-12-01

J R Perez-Polo, E M Shooter

引用次数: 0

Morphological, histochemical and chromosomal patterns of neuroblastoma parental and purine resistant lines. 神经母细胞瘤亲本系和嘌呤抗性系的形态、组织化学和染色体模式。

Neurobiology Pub Date : 1975-12-01

J Ciesielski-Treska, S Warter, P Mandel

引用次数: 0

Effects of freezing and storage on subcellular fractionation of guinea pig and human brain. 冷冻和贮藏对豚鼠和人脑亚细胞分离的影响。

Neurobiology Pub Date : 1975-12-01

W L Stahl, P D Swanson

{"title":"Effects of freezing and storage on subcellular fractionation of guinea pig and human brain.","authors":"W L Stahl, P D Swanson","doi":"","DOIUrl":"","url":null,"abstract":"Subcellular fractionations were carried out on guinea pig and human brains. Distributions of protein marker enzymes, and galactolipids were examined with guinea pig cerebral cortex that was (Group I) homogenized immediately; (Group II) stored 3 to 5 days at -70 degrees C prior to homogenization; (Group III) stored 3 to 6 months; (Group IV) homogenized after 3 h at R.T. and 16 to 18 h at 4 degrees C and then stored at -70 degrees C for 7 to 9 months. Human frontal lobe obtained at autopsy was fractionated immediately (Group V) or stored at -70 degrees C for 5 to 8 months prior to fractionation (Group VI). Protein recoveries in myelin, microsomal, synaptosomal, and supernatant fractions were decreased in brains that were not frozen for several hours prior to storage (Groups IV-VI). SDH and MAO recoveries in the nuclear and free mitochondrial fractions were increased in these groups. AChE, a membrane marker, was also increased in the free mitochondrial fractions in Groups IV-VI, suggesting increased contamination of mitochondria by synaptosomal membrane fragments. Arylsulfatase, a lysosomal enzyme, was decreased in the free mitochondrial fraction with freezing, but the distributions in tissues not frozen for several hours showed only an increase in the nuclear fraction and a decrease in the microsomal fraction. Freezing brought about an increase in supernatant LDH and a decrease in this enzyme in the free mitochondrial fractions. Total galactolipid contents in synaptosomal and free mitochondrial fractions were increased by freezing and storage. Though some redistribution of enzymes takes place, meaningful subcellular fractions can be obtained after storage of fresh and postmortem brain tissues.","PeriodicalId":19126,"journal":{"name":"Neurobiology","volume":"5 6","pages":"393-400"},"PeriodicalIF":0.0,"publicationDate":"1975-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"12384597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro study of bovine oligodendroglia. 牛少突胶质细胞的体外研究。

Neurobiology Pub Date : 1975-12-01

M E Fewster, S Blackstone

引用次数: 0

Concanavalin A-binding glycopeptides from rat brain glycoproteins. 从大鼠脑糖蛋白中提取的刀豆蛋白a结合糖肽类。

Neurobiology Pub Date : 1975-12-01

H I Hof, J P Susz, J I Javaid, E G Brunngraber

引用次数: 0

Studies on the action of nerve growth factor. II. Neurotubule protein levels during neurite outgrowth. 神经生长因子作用的研究。2神经突生长过程中的神经小管蛋白水平。

Neurobiology Pub Date : 1975-10-01

S B Mizel, J R Bamburg

引用次数: 0