Natural product sciences最新文献_第10页

Snake Venom Phospholipase A₂ and its Natural Inhibitors 蛇毒磷脂酶a2及其天然抑制剂

Natural product sciences Pub Date : 2020-12-31 DOI: 10.20307/nps.2020.26.3.259

Pushpendra Singh, Mohammad Yasir, Ruchir Khare, M. Tripathi, R. Shrivastava

{"title":"Snake Venom Phospholipase A₂ and its Natural Inhibitors","authors":"Pushpendra Singh, Mohammad Yasir, Ruchir Khare, M. Tripathi, R. Shrivastava","doi":"10.20307/nps.2020.26.3.259","DOIUrl":"https://doi.org/10.20307/nps.2020.26.3.259","url":null,"abstract":"Snakebite is a severe medical, economic, and social problem across the world, mostly in the tropical and subtropical area. These regions of the globe have typical of the world''s venomous snakes present where access to prompt treatment is limited or not available. Snake venom is a complex mixture of toxin proteins like neurotoxin and cardiotoxin, and other enzymes like phospholipase A2 (PLA2), haemorrhaging, transaminase, hyaluronidase, phosphodiesterase, acetylcholinesterase, cytolytic and necrotic toxins. Snake venom shows a wide range of biological effects like anticoagulation or platelet aggregation, hemolysis, hypotension and edema. Phospholipase A2 is the principal constituent of snake venom; it catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid, which is the precursor of eicosanoids including prostaglandins and leukotrienes. The information regarding the structure and function of the phospholipase A2 enzyme may help in treating the snakebite victims. This review article constitutes a brief description of the structure, types, mechanism occurrence, and tests of phospholipase A2 and role of components of medicinal plants used to inhibit phospholipase A2.","PeriodicalId":19080,"journal":{"name":"Natural product sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44010060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Chemicals Constituents from Leaves of Diospyros iturensis (Gürke) Letouzey & F. White and their Biological Activities 薯蓣(Diospyros iturensis, g<s:1> rke) Letouzey & F. White叶化学成分及其生物活性研究

Natural product sciences Pub Date : 2020-12-31 DOI: 10.20307/nps.2020.26.4.311

Marius Feumo Feusso Hermann, Catherine Vardamides Juliette, de dieu Dongmo Jean, Laure Maza Djomkam Hermine, Mvot Akak Carine, M. Lateef, A. Ayaz, Guy Blaise Azebaze Anatole, François Kamdem Waffo Alain, Shaiq Ali Muhammad

引用次数: 2

Characterization and Potent Application of Pleurotus floridanus Trypsin Inhibitor (PfTI) 花菇胰蛋白酶抑制剂（PfTI）的特性及其应用

Natural product sciences Pub Date : 2020-09-30 DOI: 10.20307/NPS.2020.26.3.207

M. Pannippara, S. Kesav, R. Raghavan, Abraham Mathew, S. Bhat, Elyas Kothanan Kozhiyil

{"title":"Characterization and Potent Application of Pleurotus floridanus Trypsin Inhibitor (PfTI)","authors":"M. Pannippara, S. Kesav, R. Raghavan, Abraham Mathew, S. Bhat, Elyas Kothanan Kozhiyil","doi":"10.20307/NPS.2020.26.3.207","DOIUrl":"https://doi.org/10.20307/NPS.2020.26.3.207","url":null,"abstract":"Characterization and in vitro inhibition studies of protease inhibitor from the mushroom Pleurotus floridanus (PfTI) towards the pest Papilio demoleus is studied. The addition of 1 mM Mn2+, Na2+, Ba2+ and Ni 2+ enhanced the PfTI activity. The ICP-atomic emission spectrum showed the presence of Ca2+, Mg2+ and Zn2+ in the PfTI. Surfactants SDS and CTAB at a concentration of 1% reduced the PfTI activity whereas, the nonionic detergents Triton X and Tween 80 increased the activity. The inhibitory activity gradually decreased with increase in concentration of DMSO and H2O2. The activity was increased by dithiothreitol up to a concentration of 80 μM and inactivated at 140 μM. The activity of PMSF modified PfTI was drastically reduced to 0.234 U/mL at 4 mM concentration and similar results were obtained for modification of cysteine by N-Ethylmaleimide at slightly higher concentrations. The complex of trypsin and PfTI showed complete loss in fluorescence intensity at 343 nm compared with control. In vitro inhibition studies of PfTI with midgut proteases isolated from citrus pest P. demoleus with protease activity of 1.236 U was decreased to 0.613 U by 50 μL (0.1 mg/mL) of the inhibitor. Inhibitor was stable up to 0.04 M concentration of HCl.","PeriodicalId":19080,"journal":{"name":"Natural product sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48686267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Isolation and Identification of Bioactive Compounds from the Tuber of Brassica oleracea var. gongylodes 甘蓝块茎中生物活性物质的分离与鉴定

Natural product sciences Pub Date : 2020-09-30 DOI: 10.20307/NPS.2020.26.3.214

Ritu Prajapati, S. Seong, Hyeung‐Rak Kim, H. Jung, Jae Sue Choi

引用次数: 4

Effects of Compounds from Physalis angulata on Fatty Acid Synthesis and Glucose Metabolism in HepG2 Cells via the AMP-activated Protein Kinase Pathway 角藻化合物通过AMP激活蛋白激酶途径对HepG2细胞脂肪酸合成和葡萄糖代谢的影响

Natural product sciences Pub Date : 2020-09-30 DOI: 10.20307/NPS.2020.26.3.200

Hoang Thai Hoa, N. Thu, N. Dong, T. Oanh, T. T. Hien, D. Ha

{"title":"Effects of Compounds from Physalis angulata on Fatty Acid Synthesis and Glucose Metabolism in HepG2 Cells via the AMP-activated Protein Kinase Pathway","authors":"Hoang Thai Hoa, N. Thu, N. Dong, T. Oanh, T. T. Hien, D. Ha","doi":"10.20307/NPS.2020.26.3.200","DOIUrl":"https://doi.org/10.20307/NPS.2020.26.3.200","url":null,"abstract":"The ability of the total extract from Physalis angulata; three fractions after partitioning with n-hexane, ethyl acetate (TBE), and water; and four withanolides (compounds 1 – 4) to phosphorylate 5'-adenosine monophosphate-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) in HepG2 cells was evaluated. The TBE fraction (50 μg/mL) activated p-ACC and p-AMPK expression most strongly. Compounds 1 – 4 (10 μM) upregulated p-ACC expression at different levels. Compound 4 induced the most significant changes in p-AMPK expression, followed by 1 and 2. Sterol regulatory element-binding proteins (SREBPs) play a functional role in the transcriptional regulation of the lipogenic pathway, including fatty acid synthase (FAS) and ACC. The effects of compounds 2 and 4 (10 μM) on FAS and SREBP-1c expression under high glucose conditions (30 mM) in HepG2 cells were evaluated further. Both dose-dependently inhibited FAS and SREBP-1c expression as well as lipid accumulation (1 – 10 μM) were compared to high-concentration glucose control, which upregulated FAS and SREBP-1c. These results suggest that compounds 2 and 4 upregulate AMPK, suppress FAS and SREBP-1c, and have potential effects on glucose and lipid metabolism. (Less)","PeriodicalId":19080,"journal":{"name":"Natural product sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47981815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Anti-adipogenic Pregnane Steroid from a Hydractinia-associated Fungus, Cladosporium sphaerospermum SW67 水螅相关真菌球孢枝孢SW67的抗脂肪妊娠类固醇

Natural product sciences Pub Date : 2020-09-30 DOI: 10.20307/NPS.2020.26.3.230

Lee Seoung-Rak, Kang, Hee-Sun, Yoo Min-Jeong, Yi Sang-Ah, B. Christine, Lee Jaecheol, Kim, Ki-Hyun

{"title":"Anti-adipogenic Pregnane Steroid from a Hydractinia-associated Fungus, Cladosporium sphaerospermum SW67","authors":"Lee Seoung-Rak, Kang, Hee-Sun, Yoo Min-Jeong, Yi Sang-Ah, B. Christine, Lee Jaecheol, Kim, Ki-Hyun","doi":"10.20307/NPS.2020.26.3.230","DOIUrl":"https://doi.org/10.20307/NPS.2020.26.3.230","url":null,"abstract":"A pregnane steroid, 3α-hydroxy-pregn-7-ene-6,20-dione (1), was isolated from a Hydractiniaassociated Cladosporium sphaerospermum SW67 by repetitive column chromatographic separation and highperformance liquid chromatography (HPLC) purification. The planar structure of 1 was elucidated from the analysis of the spectroscopic data (1D and 2D NMR spectra) and LC-MS data. The absolute configuration of 1 was determined by interpretation of ROESY spectrum of 1, together with the comparison of reported spectroscopic values in previous studies. To the best of our knowledge, this is the first report of the identification of the pregnane scaffold from C. sphaerospermum, a natural source. Compound 1 was evaluated for its effects on lipid metabolism and adipogenesis during adipocyte maturation and showed that compound 1 substantially inhibited lipid accumulation compared to the control. Consistently, the expression of the adipocyte marker gene (Adipsin) was reduced upon incubation with 1. Further, we evaluated the effects of 1 on lipid metabolism by measuring the transcription of lipolytic and lipogenic genes. The expression of the lipolytic gene ATGL was significantly elevated upon exposure to 1 during adipogenesis, whereas the expression of lipogenic genes FASN and SREBP1 was significantly reduced upon treatment with 1. Thus, our findings provide experimental evidence that the steroid derived from Hydractinia-associated C. sphaerospermum SW67 is a potential therapeutic agent for obesity.","PeriodicalId":19080,"journal":{"name":"Natural product sciences","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43994140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

Liver Function Analyses and Spleen Histology Assessment Following the Co-administration of Cisplatin and Methanolic Extract of Portulaca Oleracea in Wistar Rats: An Experimental Study 顺铂与马齿苋醇提物联合给药对Wistar大鼠肝功能及脾脏组织学的影响

Natural product sciences Pub Date : 2020-09-30 DOI: 10.20307/nps.2020.26.3.252

Okafor Izuchukwu Azuka, Nnamah Uchenna Somtochukwu, N. Jude

引用次数: 0

Phytochemical Constituents of Phyllanthus urinaria 叶下珠的植物化学成分

Natural product sciences Pub Date : 2020-06-30 DOI: 10.20307/NPS.2020.26.2.151

J. M. Cha, J. Park, Sang-Un Choi, K. Lee

引用次数: 1

NMR Assignments of Rotameric Aporphine Alkaloids from Liriodendron tulipifera 郁金香鹅掌楸中旋美阿啡类生物碱的核磁共振分配

Natural product sciences Pub Date : 2020-06-30 DOI: 10.20307/NPS.2020.26.2.171

InWha Park, M. Na

引用次数: 0

Butyrylcholinesterase Inhibitory Activity and GC-MS Analysis of Carica papaya Leaves 番木瓜叶的丁酰胆碱酯酶抑制活性及GC-MS分析

Natural product sciences Pub Date : 2020-06-30 DOI: 10.20307/nps.2020.26.2.165

Nelson Jeng Yeou Chear, Sathiya Maran, K. Y. Yeong, Y. Ong, B. Goh

引用次数: 4