Butyrylcholinesterase Inhibitory Activity and GC-MS Analysis of Carica papaya Leaves

Q3 Chemistry

Natural product sciences Pub Date : 2020-06-30 DOI:10.20307/nps.2020.26.2.165

Nelson Jeng Yeou Chear, Sathiya Maran, K. Y. Yeong, Y. Ong, B. Goh

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引用次数: 4

Abstract

Carica papaya is a medicinal and fruit plant owing biological activities including antioxidant, antiviral, antibacterial and anticancer. The present study aims to investigate the acetyl (AChE) and butyryl (BChE) cholinesterase inhibitory potentials of C. papaya extracts as well as their chemical compositions. The chemical composition of the active extract was identified using a gas chromatography-mass spectrometry (GCMS). Ellman enzyme inhibition assay showed that the alkaloid-enriched leaf extract of C. papaya possessed significant anti-BChE activity with an enzyme inhibition of 75.9%. GC-MS analysis showed that the alkaloid extract composed mainly the carpaine (64.9%) - a major papaya alkaloid, and some minor constituents such as aliphatic hydrocarbons, terpenes and phenolics. Molecular docking of carpaine revealed that this molecule formed hydrogen bond and hydrophobic interactions with choline binding site and acyl pocket. This study provides some preliminary findings on the potential use of C. papaya leaf as an herbal supplement for the prevention and treatment of Alzheimer’s disease.

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番木瓜叶的丁酰胆碱酯酶抑制活性及GC-MS分析

番木瓜是一种药用和果树，具有抗氧化、抗病毒、抗菌和抗癌等生物学活性。本研究旨在研究木瓜提取物的乙酰胆碱酯酶（AChE）和丁酰胆碱酯酶（BChE）抑制潜力及其化学成分。使用气相色谱-质谱法（GCMS）鉴定活性提取物的化学成分。Ellman酶抑制试验表明，富含生物碱的木瓜叶提取物具有显著的抗BChE活性，酶抑制率为75.9%。GC-MS分析表明，该生物碱提取物主要由木瓜主要生物碱carpaine（64.9%）和一些次要成分如脂肪烃、萜类和酚类组成。分子对接显示，该分子与胆碱结合位点和酰基口袋形成氢键和疏水相互作用。这项研究提供了一些关于木瓜叶作为草药补充剂预防和治疗阿尔茨海默病的潜在用途的初步发现。

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来源期刊

Natural product sciences Chemistry-Organic Chemistry

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： Natural Product Sciences is the official publication of the Korean Society of Pharmacognosy which was launched in 1995. The journal is published quarterly at the end of March, June, September, and December each year and the official title of the journal is abbreviated title as "Nat. Prod. Sci." The research papers on original work, either experimental or theoretical, that advance our understanding of natural product sciences, including important questions of phytochemistry, chemistry, and bio-chemistry of natural resources will be published. Timely reviews and commentaries on recent progress in active areas of natural products research will be also published.