Marina S Ascunce, Douglas B Booher, Aaron C Stoll, Airlan San Juan, Agnes Lee, Sanford D Porter
{"title":"First Records and Expanding Distribution of a Small Big-Headed Ant, Pheidole parva, in Florida, USA.","authors":"Marina S Ascunce, Douglas B Booher, Aaron C Stoll, Airlan San Juan, Agnes Lee, Sanford D Porter","doi":"10.1007/s13744-026-01416-4","DOIUrl":"10.1007/s13744-026-01416-4","url":null,"abstract":"<p><p>Pheidole parva Mayr (1865) is a small ubiquitous ant native to the Indomalayan region where it is considered a pest in healthcare facilities. This tramp ant was first reported outside its native range more than 100 years ago in the Seychelles Islands. Since then, it has been found in other areas of the Old World, where it was likely accidentally introduced via trading ships. In the 2000s, this ant was detected in Japan (2001), the Arabian Peninsula (2009), and recently in the Mediterranean island of Cyprus (2023) and Lebanon (2025). Here, we describe its first occurrence in North America, in the state of Florida where both Nearctic and Neotropical realms meet. Our findings are based on recent Pheidole parva specimens collected throughout the state, curated iNaturalist observations, Antweb data, and DNA barcoding based on the mitochondrial cytochrome oxidase subunit I (COI) gene. Among the 26 P. parva specimens analyzed from Florida, we found three different COI haplotypes. Two of them were also found in other introduced areas; however, one of the haplotypes has only been detected in a native population. The three haplotypes were distributed throughout the state with no clear geographical structure, consistent with multiple introductions and broad establishment of this non-native ant in Florida.</p>","PeriodicalId":19071,"journal":{"name":"Neotropical Entomology","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13388651/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148536417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Felipe Capoccia, Lílian Andrade, Marianna V P Simões, Fernando A Frieiro-Costa, João Vasconcellos-Neto
{"title":"The Tortoise Beetles of an Atlantic Forest Mountain Range: Host Plants and Temporal Distribution.","authors":"Felipe Capoccia, Lílian Andrade, Marianna V P Simões, Fernando A Frieiro-Costa, João Vasconcellos-Neto","doi":"10.1007/s13744-026-01411-9","DOIUrl":"10.1007/s13744-026-01411-9","url":null,"abstract":"<p><p>Tortoise beetles (Cassidinae s. str., Chrysomelidae, Coleoptera) compose a highly diverse group of insects, with most species found in Neotropical regions, such as the Brazilian Atlantic Forest, one of the most important hotspots in the world. Neotropical cassidines are known to typically display strong host specialization and distinct temporal patterns; however, host plants of most species are still unknown and very few studies have quantitatively investigated their temporal distribution. Here, we present a comprehensive list of tortoise beetle species for Serra do Japi, an Atlantic Forest mountain range, by comparing and combining data from 26 months of field work with data from previous samplings done in the same area. By collecting richness and abundance of adults for 12 consecutive months, as well as monthly mean temperature and precipitation, we also tested the correlation between assemblage and climatic factors. Our final list is composed of 71 cassidines species, with 11 new distribution records for the state of São Paulo and new host plant records for 36 of these species. We also show that the adults of this tortoise beetle assemblage present a seasonal pattern, similar to that of subtropical cassidines, which is significantly influenced by temperature variation, but not by precipitation. Our results highlight the importance of Serra do Japi as a conservation unit and contribute to a better understanding of several ecological aspects of Cassidinae s. str.</p>","PeriodicalId":19071,"journal":{"name":"Neotropical Entomology","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13388358/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148536435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Resident Parasitoids and Biotic Resistance to Invasive Species in Hawai'i, and Implications for Biological Control Programs.","authors":"Mark G Wright, Michelle G Au","doi":"10.1007/s13744-026-01418-2","DOIUrl":"10.1007/s13744-026-01418-2","url":null,"abstract":"<p><p>Hawai'i is subject to disproportionately high rates of biological invasion, including adventive insects. These alien species have been the targets of numerous biological control efforts. The contribution of native species preventing adventives from becoming invasive has received little attention. We analyzed numbers of invasive species utilized by native parasitoids, showing that resident natural enemies infrequently attack alien species, but in some cases, exert significant population control. Purposefully introduced biological control agents likely also contribute to mitigating new invasions. Certain taxa, such as Coleoptera, should be considered priority targets for importation biological control, as they appear highly unlikely to accumulate resident natural enemies.</p>","PeriodicalId":19071,"journal":{"name":"Neotropical Entomology","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13388341/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148536460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First Record of the Chewing Louse Trichodectes canis (Psocodea: Trichodectidae) on the Domestic Cat (Felis catus) in Central America.","authors":"Juan J Oporta-López, Adriana Troyo","doi":"10.1007/s13744-026-01419-1","DOIUrl":"10.1007/s13744-026-01419-1","url":null,"abstract":"<p><p>Trichodectes canis is a chewing louse considered highly specific to domestic and wild canids. Here, we report the first record of T. canis infesting a domestic cat (Felis catus) in Central America. In April 2025, an adult male cat from a rural community near Juigalpa, Chontales Department of Nicaragua, was brought to a veterinary clinic in critical condition, showing marked lethargy and anorexia. During clinical inspection, multiple chewing lice were observed on the animal's coat. Six specimens (five nymphs and one female) were manually collected and taxonomically identified as T. canis based on diagnostic morphological characters. The cat died a few minutes after admission. The statement of the owner as to the absence of dogs in the household and the recovery of multiple live specimens suggest a possible establishment of the lice on the feline host, rather than a momentary contamination. This finding represents a new host record for T. canis in the Neotropical region and expands knowledge on the distribution and host range of this species.</p>","PeriodicalId":19071,"journal":{"name":"Neotropical Entomology","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148536429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Weifeng Wang, Bo Xu, Xiaolan Ou, Yanhui Lu, Jingsong Kong, Guifen Zhang, Cong Huang, Yibo Zhang, Fanghao Wan
{"title":"Electrophysiological and Behavioral Responses of Tuta absoluta to the Tomato-Associated Volatile 1-Nonanol.","authors":"Weifeng Wang, Bo Xu, Xiaolan Ou, Yanhui Lu, Jingsong Kong, Guifen Zhang, Cong Huang, Yibo Zhang, Fanghao Wan","doi":"10.1007/s13744-026-01410-w","DOIUrl":"10.1007/s13744-026-01410-w","url":null,"abstract":"<p><p>The South American tomato pinworm, Tuta absoluta (Lepidoptera: Gelechiidae), is a devastating invasive pest of tomato (Solanum lycopersicum). To evaluate candidate female-oriented semiochemicals, we examined the electroantennogram (EAG) activity of ten tomato-associated volatiles selected from previous reports. The compound 1-nonanol was selected as a candidate based on its relatively strong EAG activity and was subsequently assessed for its effects on olfaction, orientation, and oviposition. These findings indicate that 1-nonanol elicits female-biased attraction and stimulates oviposition under laboratory conditions. However, its ecological relevance within natural tomato volatile blends and its field performance require further validation before it can be developed as a female-oriented IPM component.</p>","PeriodicalId":19071,"journal":{"name":"Neotropical Entomology","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148308615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Influence of Habitat and Climate on the Spatial Distribution of Outbreaks of the Hylesia metabus Moth, Responsible for Lepidopterism, in Coastal French Guiana.","authors":"Raphaël Fougeray, Isaline Orhon, Manon Denux, Romane Ibanez, Romane Leseur, Liliana Ballesteros-Mejia, Rodolphe Rougerie, Giacomo Sellan, Yi Moua, Mónica Arias, Melanie McClure","doi":"10.1007/s13744-026-01399-2","DOIUrl":"10.1007/s13744-026-01399-2","url":null,"abstract":"<p><p>Understanding the environmental context of insect outbreaks is crucial, particularly for pest species with significant impact on human health. The ashen moth Hylesia metabus is a generalist Lepidoptera, of which the females have urticating scales (setae) that cause severe dermatological reactions, and its outbreaks thus pose serious public health challenges along the coastal regions of Venezuela and French Guiana. Despite the species' broad distribution throughout northern South America, outbreaks remain unpredictable and localized. Here, we explored factors that correlate with the spatial distribution of outbreaks by investigating 13 sites in French Guiana. We assessed forest structure, tree species composition, canopy cover, and avian predation rates in the field. Additionally, we performed species distribution modeling to explore the effect of climate. Outbreak-prone sites were associated with overall low tree densities, limited daily temperature variation, pronounced seasonal changes between the dry and rain seasons, and, surprisingly, higher avian predation rates which may be more indicative of favorable landscape structure. These conditions are more prevalent along the coast of French Guiana, contrasting sharply with the stable and diverse inland rainforest ecosystems where outbreaks are rarely reported. These findings highlight habitat features consistently associated with outbreaks and provide a first step toward identifying ecological conditions that may influence outbreak propensity and can inform future monitoring strategies under changing environmental conditions.</p>","PeriodicalId":19071,"journal":{"name":"Neotropical Entomology","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13279682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148271600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alexandra Gianaris, Manuela O Ramalho, Amanda A de Oliveira, Maria Santina de Castro Morini, Cintia Martins, Odair C Bueno
{"title":"Bacterial Diversity in Leaf-Cutter Ant Species: Host-Microbe Interactions and Environmental Effects.","authors":"Alexandra Gianaris, Manuela O Ramalho, Amanda A de Oliveira, Maria Santina de Castro Morini, Cintia Martins, Odair C Bueno","doi":"10.1007/s13744-026-01386-7","DOIUrl":"10.1007/s13744-026-01386-7","url":null,"abstract":"<p><p>Historically, studies have sought to identify host-specific factors in host-microbe interactions as a means of understanding evolutionary success. The genus Atta, comprising leaf-cutter ants, is native to the southern Neotropics and obligately mutualistic with cultivated fungi. It hosts a remarkably diverse range of bacterial communities, yet this variability remains poorly understood. Using high-throughput amplicon sequencing of the 16S rRNA genes of the whole worker, we showed significant difference between the bacterial communities among 4 dominant Atta species: Atta sexdens (Linnaeus), Atta laevigata (Smith), Atta capiguara (Gonçalves), and Atta bisphaerica (Forel). We also discovered significant differences in bacterial communities from laboratory conditions, pesticide treatment, and the fungal garden symbiont of Atta sexdens. Surprisingly, bacterial communities of Atta sexdens kept in the laboratory were not significantly different from pesticide-treated Atta sexdens, laying the groundwork for potential refinement of standard research methods.</p>","PeriodicalId":19071,"journal":{"name":"Neotropical Entomology","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13260043/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148218782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Leonardo Martín Díaz-Nieto, Alfonsina Palladini, Andrea Moyano, Cristina Coria, Fernando Murúa, Valeria Pantano, Juan Rull
{"title":"Wolbachia in Tephritid Populations from Argentina: New Hosts and First Report of Multiple Infections in Rhagoletotrypeta pastranai.","authors":"Leonardo Martín Díaz-Nieto, Alfonsina Palladini, Andrea Moyano, Cristina Coria, Fernando Murúa, Valeria Pantano, Juan Rull","doi":"10.1007/s13744-026-01408-4","DOIUrl":"10.1007/s13744-026-01408-4","url":null,"abstract":"<p><p>The Tephritidae family comprises agriculturally important insect pests that cause significant economic losses worldwide. Interest in eco-friendly pest control methods is growing, and novel strategies involving symbiotic microorganisms are under investigation. Strategies involving the bacterium Wolbachia have been proposed for managing pest insects and disease vectors. However, they have not yet been used for tephritid pest control. The aim of this study was to detect and characterize Wolbachia strains in tephritid flies from Argentina that could be proposed for use in area-wide programs applying the incompatible insect technique. We also aimed to identify and analyze Wolbachia strains in little-studied species of no economic importance. Infested fruits were collected from different localities in two provinces of Argentina. Adults were identified and the presence of Wolbachia strains was evaluated using the wsp surface protein primer. Genotyping was performed through multilocus sequence typing (MLST). When multiple infections were suspected, the obtained amplification products were cloned into P-GEN. Two new Wolbachia hosts were identified, Rhagoletis blanchardi Aczél and Rhagoletotrypeta pastranai Aczél from Tucumán. Interestingly, we found a multiple infection by Wolbachia in R. pastranai, detecting three different strains. The Anastrepha fraterculus (Wiedemann) strains were similar to haplotypes previously described in Argentina. Our results provide the first records of Wolbachia strains in new tephritid hosts from Argentina. The phenotypic effects of these strains on their hosts should be further investigated to evaluate their potential for Wolbachia-based control strategies.</p>","PeriodicalId":19071,"journal":{"name":"Neotropical Entomology","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148218761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Acute Toxicity and Locomotor Effects of Oral Exposure to the Insecticide Indoxacarb in Two Species of Neotropical Stingless Bees, Melipona beecheii and Nannotrigona perilampoides.","authors":"Selmy Adriana Ayala-Tzab, Esaú Ruiz-Sánchez, Cristian Góngora-Gamboa, Horacio Ballina-Gómez, Luis Latournerie-Moreno, Alejandro García-Ramírez","doi":"10.1007/s13744-026-01407-5","DOIUrl":"10.1007/s13744-026-01407-5","url":null,"abstract":"<p><p>Indoxacarb is an oxadiazine insecticide widely used in agriculture to control lepidopteran larvae. Although it is often regarded as a selective, reduced-risk compound with limited effects on non-target arthropods, its potential impact on bees, especially native species, remains poorly understood. In this study, we evaluated the acute toxicity and locomotor effects of indoxacarb in two stingless bee species, Melipona beecheii and Nannotrigona perilampoides (Hymenoptera: Meliponini). Under laboratory conditions, bees were exposed for 48 h to sucrose solution containing the field-recommended concentration of indoxacarb (75 mg L<sup>-1</sup>), its tenfold (7.5 mg L<sup>-1</sup>) and 100-fold (0.75 mg L<sup>-1</sup>) dilutions, or an insecticide-free control. Survival, mean survival time, hazard ratios (HR), and walking speed were assessed. At the field-recommended concentration, both species reached 0% survival, within 12 h in M. beecheii and 48 h in N. perilampoides. Mean survival time was 11.7 h and 42.4 h, and the HR was 335.21 and 36.62, respectively. At the tenfold and 100-fold dilutions, survival in M. beecheii declined to 23.3% and 53.3% after 48 h, whereas N. perilampoides declined to 73.3% with both dilutions. Exposure to indoxacarb also affected locomotor performance. In M. beecheii, walking speed decreased after 6 h of exposure, with the significant reduction at the field-recommended concentration, while the lowest concentration did not differ from the control. In N. perilampoides, this effect occurred only at the field-recommended rate after 10 h. Overall, our findings show that indoxacarb poses lethal and sublethal risks to Neotropical stingless bees, with M. beecheii exhibiting greater susceptibility than N. perilampoides.</p>","PeriodicalId":19071,"journal":{"name":"Neotropical Entomology","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148150755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pedro Bonfá-Neto, Felipe Ribeiro Pereira Sarmento, Rayner Paresqui Constantino, Roberta Paresque, Frederico Falcão Salles
{"title":"Integrative Taxonomy and Life Stage Associations in Nectopsyche fuscomaculata Flint, 1983 (Trichoptera: Leptoceridae).","authors":"Pedro Bonfá-Neto, Felipe Ribeiro Pereira Sarmento, Rayner Paresqui Constantino, Roberta Paresque, Frederico Falcão Salles","doi":"10.1007/s13744-026-01406-6","DOIUrl":"10.1007/s13744-026-01406-6","url":null,"abstract":"<p><p>Semaphoronts are entities that carry the characteristics of individuals at different periods over time, essential for understanding species in various biological areas. In the long-horned caddisfly genus Nectopsyche Müller, 1879 (Trichoptera: Leptoceridae), there is a significant lack of knowledge about semaphoronts, especially in South America. Historically, descriptions have focused mainly on adult males, with descriptions of larvae, pupae, and adult females occurring in isolated studies. This study addresses the Haeckelian shortfall by associating adults and larvae of the genus Nectopsyche using mitochondrial COI gene sequences. The study was conducted in the Rio Doce hydrographic basin, southeastern Brazil, where specimens were collected and photographed. The resulting DNA sequences were compared with data available and analyzed using Neighbor-Joining methods. Our findings show that N. fuscomaculata and its congeners were recovered with high support values, presenting a barcode gap of 7% among them. As a result, the association between the adult female and larval semaphoronts with the adult male of N. fuscomaculata was established. The previously unknown semaphoronts of this species, namely the female and the larva, are described and illustrated. This study expands current knowledge of the genus' biological diversity and highlights the importance of integrative taxonomic approaches.</p>","PeriodicalId":19071,"journal":{"name":"Neotropical Entomology","volume":"55 1","pages":""},"PeriodicalIF":1.8,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13221352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148055786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}