Mycotoxins最新文献_第8页

遺伝子塩基配列を用いての Fusarium 属菌の同定と分子系統学的位置付けに関する研究利用基因碱基序列的Fusarium属菌的鉴定和分子系统学定位的研究

Mycotoxins Pub Date : 2013-07-31 DOI: 10.2520/MYCO.63.133

渡辺麻衣子

{"title":"遺伝子塩基配列を用いての Fusarium 属菌の同定と分子系統学的位置付けに関する研究","authors":"渡辺麻衣子","doi":"10.2520/MYCO.63.133","DOIUrl":"https://doi.org/10.2520/MYCO.63.133","url":null,"abstract":"Species of the genus Fusarium are well-researched in many fields, and a commonly problem by researchers interested in Fusarium species is the probable taxonomic system and the identification method of this genus. The traditional taxonomic system for fungi has been proposed based on the mainly morphological species concept, including the genus Fusarium. Recently, many researchers have applied molecular markers to examine the taxonomy and identification of Fusarium species. This review shows some recent findings from our studies about molecular phylogeny and identification of Fusarium species based on analyses with nucleotide sequences. First, the genetic markers were evaluated for identifying Fusarium isolates by calculation of the homologies with pairwise comparison of all tested strains, and of the ratio of nucleotide substitution rate. It was suggested that aminoadipate reductase gene (lys2) is notionally the most appropriate genetic marker for identifying isolates among the six genes examined. Second, actual identification of food-borne isolates of the genus Fusarium based on the nucleotide sequence homology was performed, and the results were evaluated. In terms of accuracy and ease, b-tubulin gene, not lys2, is the most useful genetic maker among the six genes examined. Finally, the genetic markers were evaluated for the phylogenetic analysis of Fusarium species. It was suggested the lys 2 have a singular evolutionary history than other genes. To obtain a reliable phylogeny for Fusarium species, the lys2 sequences were excluded from the dataset, and the species tree was inferred. The reliable Fusarium species tree was reconstructed, and some interesting relationships were newly described.","PeriodicalId":19069,"journal":{"name":"Mycotoxins","volume":"11 1","pages":"133-142"},"PeriodicalIF":0.0,"publicationDate":"2013-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84191527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

ナイジェリアにおけるゼアラレノンと T-2 トキシンによるとうもろこし汚染とヒトへの暴露尼日利亚zeallennon和T-2毒素对玉米的污染和人类的暴露

Mycotoxins Pub Date : 2013-07-31 DOI: 10.2520/MYCO.63.143

C. G. Afolabi, E. J. A. Ekpo, Ranajit Bandyopadhyay

引用次数: 5

沖縄の伝統発酵食品―豆腐ようの歴史，発酵と機能性冲绳传统发酵食品——豆腐用的历史，发酵与功能性

Mycotoxins Pub Date : 2013-01-31 DOI: 10.2520/MYCO.63.67

正昭安田

引用次数: 0

Genome engineering of Fusarium species by using positive and negative selection approaches for studying regulation of mycotoxin production 利用正选择和负选择方法对镰刀菌进行基因组工程，研究真菌毒素产生的调控

Mycotoxins Pub Date : 2013-01-31 DOI: 10.2520/MYCO.63.85

Y. Nakajima, Hinayo Ichikawa, Kazuyuki Maeda, T. Nishiuchi, Tetsuo Kobayashi, M. Kimura

引用次数: 1

Occurrence, mycotoxins and toxicity of Fusarium species from Abelmoschus esculentus and Sesamum indicum seeds 青豆和芝麻种子镰刀菌的发生、真菌毒素和毒性

Mycotoxins Pub Date : 2013-01-31 DOI: 10.2520/MYCO.63.27

C. Ezekiel, C. Nwangburuka, G. Chioma, M. Sulyok, B. Warth, C. Afolabi, Adenike A. Osiberu, Michael O. Oladimeji, O. A. Denton, G. Tayo, R. Krska

{"title":"Occurrence, mycotoxins and toxicity of Fusarium species from Abelmoschus esculentus and Sesamum indicum seeds","authors":"C. Ezekiel, C. Nwangburuka, G. Chioma, M. Sulyok, B. Warth, C. Afolabi, Adenike A. Osiberu, Michael O. Oladimeji, O. A. Denton, G. Tayo, R. Krska","doi":"10.2520/MYCO.63.27","DOIUrl":"https://doi.org/10.2520/MYCO.63.27","url":null,"abstract":"Seeds of two okra accessions and 17 samples of sesame seeds were examined for contamination by Fusarium species. Altogether, 37 Fusarium isolates were obtained from the two crops: 6 isolates from two okra accessions and 31 isolates from 10 sesame samples. The isolates belonged to three species: F. oxysporum, F. semitectum and F. verticillioides. All isolates from okra were identified as F. semitectum while the three species of Fusarium occurred in sesame. Six randomly selected isolates from the two crops were screened for their ability to produce mycotoxins in ofada rice culture and the crude extracts of the mycotoxins were tested on week-old Clarias gariepinus fingerlings. Six metabolites were produced by the isolates in ofada rice: equisetin (EQUS), fumonisin B1 (FB1), FB2, methyl-equisetin (M-EQUS), moniliformin (MON) and zearalenone (ZEA). All isolates produced EQUS at concentrations ranging 454-29,983 μg/kg. All isolates except F. semitectum BUFC 041 and F. oxysporum BUFC 024 produced MON and ZEA, while three F. semitectum isolates produced M-EQUS. Only F. verticillioides isolates produced fumonisins. There was evidence of variation within species obtained from both crops based on toxin profile and level of toxin produced. The culture extracts of all isolates induced 100% lethality to C. gariepinus fingerlings except for extracts of F. semitectum BUFC 041 which killed only 62.2% of the fingerlings, possibly due to the absence of MON. Our data suggest that okra and sesame may be potential sources of toxigenic Fusarium.","PeriodicalId":19069,"journal":{"name":"Mycotoxins","volume":"38 1","pages":"27-38"},"PeriodicalIF":0.0,"publicationDate":"2013-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90587271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Food poisoning cases caused by natural toxins in Okinawa prefecture 冲绳县天然毒素引起的食物中毒病例

Mycotoxins Pub Date : 2013-01-31 DOI: 10.2520/MYCO.63.55

Koji Tamanaha

引用次数: 0

Alternaria species and mycotoxins associated to black point of cereals 与谷物黑斑病有关的交替菌种类和真菌毒素

Mycotoxins Pub Date : 2013-01-31 DOI: 10.2520/MYCO.63.39

M. T. Amatulli, F. Fanelli, A. Moretti, G. Mulè, A. Logrieco

引用次数: 19

Fusarium graminearum の Tri4 遺伝子のプロモーターをトリコテセン生合成遺伝子クラスターの末端に配置すると機能しない将Fusarium graminearum的Tri4基因的启动子放置在生物合成基因簇的末端，它就会失效。

Mycotoxins Pub Date : 2013-01-31 DOI: 10.2520/MYCO.63.17

武史東海, 佑一中嶋, 雛代市川, 一行前田, 巧西内, 哲夫小林, 真木村

引用次数: 1

カビ毒国際会議 WMF meets IUPAC 2012 参加報告カビ毒国际会议 WMF meets IUPAC 2012 参加报告

Mycotoxins Pub Date : 2013-01-31 DOI: 10.2520/MYCO.63.93

博之中川

引用次数: 0

Use of cyclodextrin-based polymer for patulin analysis in apple juice 用环糊精聚合物分析苹果汁中的展霉素

Mycotoxins Pub Date : 2013-01-01 DOI: 10.2520/MYCO.63.1

Takashi Shirasawa, M. Ueda, M. Appell, T. Goto

{"title":"Use of cyclodextrin-based polymer for patulin analysis in apple juice","authors":"Takashi Shirasawa, M. Ueda, M. Appell, T. Goto","doi":"10.2520/MYCO.63.1","DOIUrl":"https://doi.org/10.2520/MYCO.63.1","url":null,"abstract":"Penicillium expansum, one of the patulin producing fungi that causes decay on apple, is recognized as the main source of patulin contamination on apple and apple products. The widely used method for patulin analysis in apple juice is liquid-liquid extraction with ethyl acetate followed by HPLC-UV or LC-MS detection. Previous studies have shown cyclodextrin polymers to exhibit favorable adsorption properties for several classes of small organic molecules, including patulin in apple juice. In this study, an insoluble polymer composed of cyclodextrin crosslinked with 4 ,4 ʼ -methylenebis(phenyl isocyanate) was synthesized for use in the solid phase extraction of patulin from apple juice. Conditions investigated for this method were solvent for column conditioning, sample volume to load patulin on the column, solvent for washing, and solvent and volume for patulin elution and optimized recovery of patulin from the column. At the optimized conditions, the recovery and relative standard deviation (RSD) of patulin from apple juice spiked at 10, 20, 50, 80 and 100 ng mL were 78 and 20 %, 71 and 13 %, 78 and 17 %, 71 and 7.1 %, 67 and 2.9 %, respectively. Limit of quantitation (LOQ) of patulin in apple juice by this method was 10 ng mL.","PeriodicalId":19069,"journal":{"name":"Mycotoxins","volume":"36 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77963139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12