Moscow University Biological Sciences Bulletin最新文献_第2页

Oligoribonucleotide-Containing Nanocomplexes Based on Aminopropylsilanol Nanoparticles as Effective Inhibitors of Influenza A Virus Replication 基于氨基丙基硅烷醇纳米颗粒的含寡核苷酸纳米复合物是甲型流感病毒复制的有效抑制剂

Moscow University Biological Sciences Bulletin Pub Date : 2024-03-25 DOI: 10.3103/s0096392524600431

M. N. Repkova, O. Yu. Mazurkov, E. I. Filippova, M. A. Procenko, N. A. Mazurkova, B. P. Chelobanov, A. S. Levina, V. F. Zarytova

{"title":"Oligoribonucleotide-Containing Nanocomplexes Based on Aminopropylsilanol Nanoparticles as Effective Inhibitors of Influenza A Virus Replication","authors":"M. N. Repkova, O. Yu. Mazurkov, E. I. Filippova, M. A. Procenko, N. A. Mazurkova, B. P. Chelobanov, A. S. Levina, V. F. Zarytova","doi":"10.3103/s0096392524600431","DOIUrl":"https://doi.org/10.3103/s0096392524600431","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Titanium dioxide or aminopropylsilanol nanoparticles were shown to be effective vehicles for delivering oligodeoxyribonucleotides and deoxyribozymes to cells to affect target nucleic acids. In this paper, the proposed principle of the delivery has been implemented in relation to oligoribonucleotides (ORN), components of short interfering RNAs (siRNAs). It has been shown that the obtained ORN-containing nanocomplexes (Si~NH2·ORN) based on aminopropylsilanol nanoparticles penetrate eukaryotic cells. These nanocomplexes have been investigated as agents for suppressing the replication of influenza A virus (IAV) in the cellular system. It has been shown that the ORN strands targeted to (+)RNA and (–)RNA of the IAV 5th segment reduces the titer of the virus by 99.7% and 98.4%, respectively. Thus, oligoribonucleotides in the Si~NH2·ORN nanocomplexes effectively inhibit the replication of the influenza A virus.","PeriodicalId":19004,"journal":{"name":"Moscow University Biological Sciences Bulletin","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140302747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme and Biosurfactant Production by Exopolysaccharide Producing Bacteria Isolated from Cassava Peel Heaps 从木薯皮堆中分离出的产外多糖细菌产生的酶和生物表面活性剂

Moscow University Biological Sciences Bulletin Pub Date : 2024-03-25 DOI: 10.3103/s0096392523600254

J. A. Amao, P. F. Omojasola, A. A. Ayandele, A. G. Adewoyin

{"title":"Enzyme and Biosurfactant Production by Exopolysaccharide Producing Bacteria Isolated from Cassava Peel Heaps","authors":"J. A. Amao, P. F. Omojasola, A. A. Ayandele, A. G. Adewoyin","doi":"10.3103/s0096392523600254","DOIUrl":"https://doi.org/10.3103/s0096392523600254","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>A major one among bacteria used in the commercial production of enzymes is Bacillus sp.; others include Escherichia sp., Pseudomonas, Proteus, Serratia, and Rhizobium, which also yield an appreciable quantity of enzymes. Biosurfactants naturally play a vital role in the teeming motility of microbes and contribute to cellular physiological procedures of signalling and, differentiation, and biofilm formation. The sourcing and selection of microbes from natural sources have endlessly been shown to be an effective way of identifying isolates of industrial value. The current study aimed to determine the ability of exopolysaccharide-producing bacteria isolated from cassava peel heap to produce some extracellular enzymes and biosurfactants. Thirteen bacteria earlier isolated from cassava peel heaps samples and found to be exopolysaccharide producers were used in this study. The highest xylanase activity was recorded at 40°C by B. amyloliquefaciens (J47). Titratable acid content was highest in peel fermented with isolate Lactobacillus plantarum (J18). In contrast, the pH of fermented peel fell below pH 7, with the lowest being the peel fermented by Pectobacterium carotovorum (J36). Three Bacillus spp. (Bacillus sp. J1, Bacillus subtilis J2, and B. amyloliquefaciens J47) were able to produce all the three enzymes (xylanase, cellulase, and β-glucosidase) assayed for. Bacterial isolates from cassava peel heap can be helpful in many industrial processes with their ability to produce substances of industrial value.","PeriodicalId":19004,"journal":{"name":"Moscow University Biological Sciences Bulletin","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140302748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rheological Behavior of Polysaccharide Hydrogels of Alginate Reinforced by a Small Amount of Halloysite Nanotubes for Extrusion 3D Printing 海藻酸多糖水凝胶的流变行为--以少量海泡石纳米管为增强材料，用于挤压式三维打印

Moscow University Biological Sciences Bulletin Pub Date : 2024-03-11 DOI: 10.3103/s0096392523700268

V. S. Molchanov, S. A. Glukhova, O. E. Philippova

引用次数: 0

Patterns of a Disulfide Bond Formation in Toxins of Spiders from the Ctenidae Family: A Comparison with Predictions of the AlphaFold 2.0 Neural Network 栉水母科蜘蛛毒素中二硫键的形成模式：与 AlphaFold 2.0 神经网络预测的比较

Moscow University Biological Sciences Bulletin Pub Date : 2024-03-11 DOI: 10.3103/s0096392523700141

P. A. Mironov, Z. O. Shenkarev

{"title":"Patterns of a Disulfide Bond Formation in Toxins of Spiders from the Ctenidae Family: A Comparison with Predictions of the AlphaFold 2.0 Neural Network","authors":"P. A. Mironov, Z. O. Shenkarev","doi":"10.3103/s0096392523700141","DOIUrl":"https://doi.org/10.3103/s0096392523700141","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Venoms of wandering or running spiders from the family Ctenidae are multicomponent and include >500 identified peptides and proteins called ctenitoxins. The main components of the venom are cysteine-rich peptides containing an inhibitor cystine knot (ICK) motif. The pharmacological diversity of ctenitoxins makes it possible to consider some of them as prototypes for the development of new drugs to treat chronic pain, Huntington’s disease, erectile dysfunction, and glaucoma. Based on the localization of cysteine residues in the amino acid sequence, ctenitoxins are divided into 14 groups containing 6–14 Cys residues. To date, the spatial structure of only one ctenitoxin, ω-CNTX-Pn4a (Pha1β or Tx3-6) from the Brazilian wandering spider Phoneutria nigriventer, has been determined. Another ten structural groups of ctenitoxins are homologous to the known spatial structures of toxins from other spider families or other proteins; finally, three more ctenitoxin groups do not have any known structural homologues. In this study, we proposed possible patterns for the disulfide bond formation in all ctenitoxin groups. A comparison of the obtained schemes with the predictions of the AlphaFold 2.0 algorithm shows that this neural network does not always correctly predict the structure of cysteine-rich peptides, especially in the case of modeling of mature peptides without leader sequences.","PeriodicalId":19004,"journal":{"name":"Moscow University Biological Sciences Bulletin","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140097728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Structural Heterogeneity and Diversity of Bacillus subtilis 168 Biofilms under Different Conditions 不同条件下枯草芽孢杆菌 168 生物膜的结构异质性和多样性

Moscow University Biological Sciences Bulletin Pub Date : 2024-03-11 DOI: 10.3103/s0096392523700190

T. Osire, Y. Wang, O. Burtseva, O. S. Sokolova

引用次数: 0

Cryoconveyor Protocols in Correlative Light and Electron Microscopy: From Multilevel Imaging to Modeling the Biophysical Effects and “Cryotheranostics” 相关光镜和电子显微镜中的低温输送规程：从多层次成像到生物物理效应建模和 "低温对抗 "技术

Moscow University Biological Sciences Bulletin Pub Date : 2024-03-11 DOI: 10.3103/s0096392523700244

O. V. Gradov

{"title":"Cryoconveyor Protocols in Correlative Light and Electron Microscopy: From Multilevel Imaging to Modeling the Biophysical Effects and “Cryotheranostics”","authors":"O. V. Gradov","doi":"10.3103/s0096392523700244","DOIUrl":"https://doi.org/10.3103/s0096392523700244","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>This paper is a technical and methodological note aimed to introduce into the practice of biological research methods a cryomicroscopy in a conveyor mode from small magnifications to the limits of magnification/resolution of scanning electron cryomicroscopy (CryoSEM). The protocol described can be applied to the samples of a low sample preparation complexity without ultratomy or processing typical of transmission electron microscopy. According to this protocol, samples are analyzed in a single microcuvette (chip) indexed by laboratory information management system and sequentially transferred from the nondestructive low-resolution optical microscopy instruments, such as lensless cryo-microscopes, to the CryoSEM/CryoESEM level (in programmable environments and atmospheres). Methods that were introduced and tested include correlative lensless cryomicroscopy and CryoSEM (including those with the sequential transition to microanalysis on wavelength-dispersive X-ray spectrometer on the Rowland circle) as well as microscopy and microinterferometry methods in the ranges from infrared to far-ultraviolet. Among the advantages of the cryoconveyor analysis protocols are sample preservation in a single portable cuvette-chip along with a possibility to establish spatial colocalization between data of optical and electron microscopy using pattern recognition software (all the way to indexing in the laboratory information system) in conducting a full range of microscopic examination. An opportunity is additionally provided for a comprehensive nondestructive sample analysis in a sequential study of microscopic systems with a possibility of variation at the subsequent stages of high-resolution microscopy, depending on the results obtained at the preceding stages of microscopy (at lower resolution).","PeriodicalId":19004,"journal":{"name":"Moscow University Biological Sciences Bulletin","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140097926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryo-Electron Microscopy at the Biological Faculty of Moscow State University 莫斯科国立大学生物系的冷冻电镜技术

Moscow University Biological Sciences Bulletin Pub Date : 2024-03-11 DOI: 10.3103/s0096392523700232

A. V. Moiseenko, A. M. Egorov, K. V. Shaitan, O. S. Sokolova

引用次数: 0

Structural Study of the Candida auris Ribosome 念珠菌核糖体结构研究

Moscow University Biological Sciences Bulletin Pub Date : 2024-03-11 DOI: 10.3103/s0096392523700220

A. A. Atamas, A. I. Guskov, A. V. Rogachev

引用次数: 0

Growth of Mesenchymal Stem Cells on Oriented Microstructured Films and Electrospun Scaffolds 间充质干细胞在定向微结构薄膜和电纺支架上的生长

Moscow University Biological Sciences Bulletin Pub Date : 2024-03-11 DOI: 10.3103/s0096392523700189

I. V. Demianova, E. A. Akoulina, I. I. Zharkova, V. V. Voinova, D. V. Chesnokova, A. M. Hossain, T. K. Makhina, G. A. Bonartseva, V. I. Kulikouskaya, V. V. Nikalaichuk, Yu. R. Mukhortova, A. S. Pryadko, M. A. Surmeneva, R. A. Surmenev, K. V. Shaitan, A. P. Bonartsev

引用次数: 0

Single-Molecule Imaging of Ion Channels Isolated in Detergent and Styrene-Maleic Acid Copolymer 分离在洗涤剂和苯乙烯-马来酸共聚物中的离子通道的单分子成像

Moscow University Biological Sciences Bulletin Pub Date : 2024-03-11 DOI: 10.3103/s0096392523700177

A. A. Glukhova, G. Lu, K. V. Shaitan, D. V. Bagrov, G. S. Glukhov

引用次数: 0