Single-Molecule Imaging of Ion Channels Isolated in Detergent and Styrene-Maleic Acid Copolymer
Q3 Agricultural and Biological Sciences
引用次数: 0
Abstract
In this work we purified Shaker ion channel using two alternative techniques – the detergent-based (CHAPS) and styrene-maleic acid copolymer lipid particles (XIRAN-based). Using XIRAN-based technique, we can obtain higher yield of purified protein. We used atomic force microscopy and transmission electron microscopy methods to visualize the purified single molecules. Atomic force microscopy allows measuring the diameter of the single molecules but does not reveal any high-resolution details. The sizes of individual Shaker molecules isolated by the two purification methods were close to each other.
分离在洗涤剂和苯乙烯-马来酸共聚物中的离子通道的单分子成像
摘要 在这项工作中，我们使用了两种可供选择的技术来纯化振动台离子通道--基于去垢剂（CHAPS）和苯乙烯-马来酸共聚物脂质颗粒（基于 XIRAN）。使用基于 XIRAN 的技术，我们可以获得更高产率的纯化蛋白质。我们使用原子力显微镜和透射电子显微镜方法来观察纯化的单分子。原子力显微镜可以测量单分子的直径，但不能显示任何高分辨率的细节。两种纯化方法分离出的单个振动筛分子的大小接近。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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来源期刊
Moscow University Biological Sciences Bulletin
Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.00
自引率
0.00%
发文量
18
期刊介绍：
Moscow University Biological Sciences Bulletin is forum for research in all important areas of modern biology. It publishes original work on qualitative, analytical and experimental aspects of research. The scope of articles to be considered includes plant biology, zoology, ecology, evolutionary biology, biophysics, genetics, genomics, proteomics, molecular biology, cell biology, biochemistry, endocrinology, immunology, physiology, pharmacology, neuroscience, gerontology, developmental biology, bioinformatics, bioengineering, virology, and microbiology.
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