Nano Biomedicine and Engineering最新文献_第9页

Preparation of Magnesium Oxide Nanoparticles and Study Its Loaded With Recombinant Human Erythropoietin alfa Drug 氧化镁纳米颗粒的制备及其负载重组人促红细胞生成素α药物的研究

Nano Biomedicine and Engineering Pub Date : 2022-11-17 DOI: 10.5101/nbe.v14i2.p186-191

F. F. Al-Kazazz, S. Jaber, A. H. Mohammed, Amer Abdullah, Mustafa M.Kadhim, Ameera Sultan

{"title":"Preparation of Magnesium Oxide Nanoparticles and Study Its Loaded With Recombinant Human Erythropoietin alfa Drug","authors":"F. F. Al-Kazazz, S. Jaber, A. H. Mohammed, Amer Abdullah, Mustafa M.Kadhim, Ameera Sultan","doi":"10.5101/nbe.v14i2.p186-191","DOIUrl":"https://doi.org/10.5101/nbe.v14i2.p186-191","url":null,"abstract":"Loading rHuEPO-alpha drug on the surface of magnesium oxide nanoparticles further improves the effect of the drug as a treatment for anemia. After preparation of magnesium oxide nanoparticles by precipitation method, it was diagnosed by X-ray diffraction and Transmission Electron Microscopy. the loaded was diagnosed by Docking program, UV-Visible and Transmission Electron Microscopy. The study of LD50 was for thirty male mice. Magnesium Oxide nanoparticles had an average crystallite size of 8.42 nm. oral LD50 test was 896 mg/kg. Docking results presented a high energy binding with force linkage due to the link of the oxygen atom of MgO NPs with three hydrogen bonds of amino acids in the rHuEPO-alpha structure. The UV-Visible result was certain that all rHuEPO-alpha drug was loaded on the surface of MgO NPs. TEM shows that the average particle size was 9.94 nm after loaded of the rHuEPO-alpha drug on MgO NPs while was 9 nm before the loaded. Magnesium oxide nanoparticles may exhibit an effect on increasing the efficacy of rHuEPO-alpha after the loaded, leading to elevated hemoglobin and reduced anemia disease.","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46148812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNAi Degrades Spike Protein Gene Transcripts of the SARS-CoV-2 for Developing Drugs to Treat of COVID-19 RNAi降解SARS-CoV-2刺突蛋白基因转录本用于开发治疗COVID-19的药物

Nano Biomedicine and Engineering Pub Date : 2022-11-07 DOI: 10.5101/nbe.v14i2.p173-185

Weiwei Zhang, Linjia Huang, Jumei Huang, Xin Jiang, Xiaohong Ren, X. D. Shi, Ling Ye, Shuhui Bian, Jianhe Sun, Yufeng Gao, Zehua Hu, Lintin Guo, Suyan Chen, Jiahao Xu, Jie Wu, Jiwen Zhang, Daxiang Cui, Fangping Dai

{"title":"RNAi Degrades Spike Protein Gene Transcripts of the SARS-CoV-2 for Developing Drugs to Treat of COVID-19","authors":"Weiwei Zhang, Linjia Huang, Jumei Huang, Xin Jiang, Xiaohong Ren, X. D. Shi, Ling Ye, Shuhui Bian, Jianhe Sun, Yufeng Gao, Zehua Hu, Lintin Guo, Suyan Chen, Jiahao Xu, Jie Wu, Jiwen Zhang, Daxiang Cui, Fangping Dai","doi":"10.5101/nbe.v14i2.p173-185","DOIUrl":"https://doi.org/10.5101/nbe.v14i2.p173-185","url":null,"abstract":"COVID-19 is caused by severe acute respiratory SARS-CoV-2. Regardless of the availability of treatment strategies for COVID-19, effective therapy will remain essential. A promising approach to tackle the SARS-CoV-2 could be small interfering (si) RNAs. Here we designed the small hairpin RNA (named as shRNA688) for targeting the prepared 813 bp Est of the S protein genes (Delta). The conserved and mutated regions of the S protein genes from the genomes of the SARS-CoV-2 variants in the public database were analyzed. A 813 bp fragment encoding the most part of the RBD and partial downstream RBD of the S protein was cloned into the upstream red florescent protein gene (RFP) as a fusing gene in the pCMV-S-Protein RBD-Est-RFP plasmid for expressing a potential target for RNAi. The double stranded of the DNA encoding for shRNA688 was constructed in the downstream human H1 promoter of the plasmid in which CMV promoter drives enhanced green fluorescent protein (EGFP) marker gene expression. These two kinds of the constructed plasmids were co-transfected into HEK293T via Lipofectamine 2000. The degradation of the transcripts of the SARS-CoV-2 S protein fusing gene expressed in the transfected HEK293T treated by RNAi was analyzed by RT-qPCR with a specific probe of the targeted SARS-CoV-2 S protein gene transcripts. Our results showed that shRNA688 targeting the conserved region of the S protein genes could effectively reduce the transcripts of the S protein genes. This study provides a cell model and technical support for the research and development of the broad-spectrum small nucleic acid RNAi drugs against SARS-CoV-2 or the RNAi drugs for the other hazard viruses which cause human diseases.","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":"14 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciopen.com/article_pdf/1633005221057290241.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Nano Cinnamon: A Study in Human Blood Medium Using Cyclic Voltammetry on Glassy Carbon Electrode (GCE) 纳米肉桂在人血培养基中的玻碳电极循环伏安法研究

Nano Biomedicine and Engineering Pub Date : 2022-10-23 DOI: 10.5101/nbe.v14i2.p167-172

M. Radhi, A. Ibrahim, M. Jabir, E. Al-Mulla, W. H. Hoidy

引用次数: 0

A Molecular Dynamic Approach to a Theory on the Dynamical Behaviour of HIV (3LPU Protein) - Water Molecules Interactions in Atomic Structures HIV（3LPU蛋白）-水分子在原子结构中相互作用动力学行为理论的分子动力学方法

Nano Biomedicine and Engineering Pub Date : 2022-10-22 DOI: 10.5101/nbe.v14i2.p159-166

Zahra Karimi, Shokoufeh Heydaripour, Rozita Farhadi, Bita Farhadi, Fariba Karami Moghadam, M. Omidi

引用次数: 6

The Anti-proliferative Activity of Factory Wastes Nanoparticles against Uterus Cancer Cells: In-vitro Study 工厂废料纳米粒子对癌症子宫细胞的抗增殖活性研究

Nano Biomedicine and Engineering Pub Date : 2022-10-21 DOI: 10.5101/nbe.v14i2.p149-158

N. Ghdeeb, A. H. Mohammed, A. R. A. Majeed

{"title":"The Anti-proliferative Activity of Factory Wastes Nanoparticles against Uterus Cancer Cells: In-vitro Study","authors":"N. Ghdeeb, A. H. Mohammed, A. R. A. Majeed","doi":"10.5101/nbe.v14i2.p149-158","DOIUrl":"https://doi.org/10.5101/nbe.v14i2.p149-158","url":null,"abstract":"As a result of increasingly rigorous environmental legislation and industry adoption of waste minimization recommendations, the development of recycling and reuse possibilities for metal-related trash has become increasingly important.Inthis research, calcium oxide and mixture of calcium, iron, and magnesium oxides was extracted from the wastes of cement factories using hydrofluoric acid. As a result, this research provides a comprehensive picture of waste recycling, and reuse throughout extraction and it examines the current state and potential developments in mineral and metal waste recycling and reusing them in different applications, among these applications, it is used CaO and CaO:MgO:Fe2O3 NPs in medical applications, including uterine cancer. Characterizations of the extracted nano-products were done using (XRD) which confirmed the crystalline nature of CaO and the mixture of CaO:MgO:Fe2O3 NPs. More, (FESEM) has been used to demonstrate the morphology of the nanostructures of CaO and the mixture CaO:MgO:Fe2O3 NPs. The data yielded the average grain size of CaO and the mixture CaO:MgO:Fe2O3 NPs calculated by SEM and XRD (64.08, 84.2) nm and (38.636, 31.597) nm respectively. (EDS) spectrum and XRD pattern suggested that prepared CaO and the mixed CaO:MgO:Fe2O3 NPs were highly pure. In addition, the ability of NPs as a promising anticancer agent was tested against MES-SA cells line, according to the MTT assay results of MESSA cells the IC50 (91.65 and 98.4) μg/mL for CaO and CaO:MgO:Fe2O3 NPs respectively. The results showed that the extracted nanoparticles CaO and the mixture CaO:MgO:Fe2O3 NPs served as an anticancer agent.","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44724684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual Degradation of Hexavalent Chromium (VI) and Cotton Blue Dye by Reduced and PVP-capped Silver Nanoparticles Using Fruit Extract of Ficus carica 以无花果果实提取物为原料，还原pvp包覆纳米银对六价铬(VI)和棉蓝染料的双重降解

Nano Biomedicine and Engineering Pub Date : 2022-10-20 DOI: 10.5101/nbe.v14i2.p123-135

Rama Jyothi Narjala, Susmila Aparna Gaddam, Sangeeta C. Casimeer, Siva Gayathri Velakanti, Ramamurthy Nadipi, Vishali Batyala, L. N. Suvarapu, V. Kotakadi

引用次数: 1

DFT-Quantum Chemical and Experimental Studies of a New 2-(Substituted Thio) Furan as a Corrosion Inhibitor in Acidic Media 新型2-(取代硫代)呋喃在酸性介质中缓蚀剂的dft -量子化学及实验研究

Nano Biomedicine and Engineering Pub Date : 2022-10-20 DOI: 10.5101/nbe.v14i2.p136-148

Israa M. H. Al-mousawi, N. A. Khudhair, Lama S. Ahmed

引用次数: 1

Metals Concentrations in Blood and Urine Measured Using a New Nano Metals Oxide Electrode 使用新型纳米金属氧化物电极测量血液和尿液中的金属浓度

Nano Biomedicine and Engineering Pub Date : 2022-09-19 DOI: 10.5101/nbe.v14i2.p107-113

E. Abood

引用次数: 5

Influence of Co Transplantation SnO2 Nano Film on the Structural and Optical Properties Using Radio Frequency Magnetron Sputtering 射频磁控溅射Co移植SnO2纳米膜对结构和光学性能的影响

Nano Biomedicine and Engineering Pub Date : 2022-01-07 DOI: 10.5101/nbe.v14i1.p1-6

E. Hassan, Mohamed Dawod, Zainab Kadhom Hamzh, M. Hassan

{"title":"Influence of Co Transplantation SnO2 Nano Film on the Structural and Optical Properties Using Radio Frequency Magnetron Sputtering","authors":"E. Hassan, Mohamed Dawod, Zainab Kadhom Hamzh, M. Hassan","doi":"10.5101/nbe.v14i1.p1-6","DOIUrl":"https://doi.org/10.5101/nbe.v14i1.p1-6","url":null,"abstract":"In this research, investigated characterization of pure and Cobalt doped Tin dioxide SnO2:Co with 3, 5 and 7 wt% fabricated using radio frequency magnetron sputtering method deposited on glass surfaces. The results showed that prepared SnO2:Co were nano films and poly crystalline in form with favored reflection permanently (110) plan, and the crystallite size decreases as the Co concentration increased 9-14 nm. The optical properties represented by the transmittance of perspicuous and cobalt transplantation SnO2 layers were studied and results showed that highest transmittance obtained was 91% in the pure films and decreased to 78% as the Co concentration increased; the wavelength range was 300-900 nm due to be the increasing of the Co amount during the deposition, leading to a linear increase in mobility and carrier concentration, until a threshold of Co content was overcome and from that point onward the mobility began to decrease. Optical energy gaps of perspicuous and Co transplantation SnO2 nano layers were determined and the energy gap was reduced from 3.50 eV of perspicuous nano layers to 3.29 eV for the highest transplantation concentration.","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44415427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capsaicin Nanoparticles as Therapeutic Agents against Gliomas 辣椒素纳米粒子对胶质瘤的治疗作用

Nano Biomedicine and Engineering Pub Date : 2021-12-16 DOI: 10.5101/nbe.v13i4.p433-445

Evelin Martínez-Benavidez, I. Higuera‐Ciapara, S. Herrera-Rodríguez, Ofelia Lugo-Melchor, F. Goycoolea, F. G. Jazo

{"title":"Capsaicin Nanoparticles as Therapeutic Agents against Gliomas","authors":"Evelin Martínez-Benavidez, I. Higuera‐Ciapara, S. Herrera-Rodríguez, Ofelia Lugo-Melchor, F. Goycoolea, F. G. Jazo","doi":"10.5101/nbe.v13i4.p433-445","DOIUrl":"https://doi.org/10.5101/nbe.v13i4.p433-445","url":null,"abstract":"Capsaicin is an alkaloid molecule with outstanding biological activity. Several reports have shown that capsaicin exerts significant antitumoral effects in several cancer cell lines, including gliomas. However, its application has been very limited due to its hydrophobicity, low affinity, and short life span. Gliomas are a heterogeneous group of brain malignant tumors with increasing prevalence worldwide. Standard therapy against these tumors generally includes resection by surgery, radiation, and chemotherapy or their combination. However, elicitation of tumor resistance to chemical or radiation treatments remains one of the main challenges to be resolved, particularly in the case of glioblastomas. Nanotechnology is an innovative approach to the treatment of Central Nervous System diseases and especially to gliomas treatment. Indeed, the use of nanotherapeutic formulations offers several advantages over the conventional methods of drug delivery therapy. In this review, we analyzed the current literature regarding the development of capsaicin-loaded nanoparticles as a promising approach for the treatment of malignant brain tumors.","PeriodicalId":18971,"journal":{"name":"Nano Biomedicine and Engineering","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49578214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0