Mucosal Immunology最新文献

Spatially separated epithelium-associated and lamina propria neutrophils present distinct functional identities in the inflamed colon mucosa.

IF 7.9 2区医学

Mucosal Immunology Pub Date : 2025-03-06 DOI: 10.1016/j.mucimm.2025.02.008

Lenore K Yalom, Caroline J Herrnreiter, Triet M Bui, Joey Lockhart, Enzo B Piccolo, Xingsheng Ren, Cenfu Wei, Anastasiia Serdiukova, Edward B Thorp, Parambir S Dulai, Ronen Sumagin

{"title":"Spatially separated epithelium-associated and lamina propria neutrophils present distinct functional identities in the inflamed colon mucosa.","authors":"Lenore K Yalom, Caroline J Herrnreiter, Triet M Bui, Joey Lockhart, Enzo B Piccolo, Xingsheng Ren, Cenfu Wei, Anastasiia Serdiukova, Edward B Thorp, Parambir S Dulai, Ronen Sumagin","doi":"10.1016/j.mucimm.2025.02.008","DOIUrl":"https://doi.org/10.1016/j.mucimm.2025.02.008","url":null,"abstract":"Inflammatory Bowel Disease (IBD) features en masse neutrophil (PMN) infiltration of the colon tissue, where PMNs occupy spatially distinct niches, including the lamina propria mucosa (LPNs) and the crypt epithelium (epithelium-associated neutrophils or EANs). Spatial PMN localization is currently used as a clinical disease scoring parameter, and EAN presence has been correlated with disease severity prognosis and reduced response to therapy. Surprisingly, although PMN heterogeneity and their clinical relevance in IBD is now well-recognized, localization-driven PMN specialization has not been investigated. We found that following initial PMN influx during the active disease phase, EANs were near-completely resolved in both UC remission patients and in murine colitis, whereas LPNs persisted throughout the resolution phase, implicating EANs as likely drivers of disease. Local profiling of transcriptional programs (by murine and human single-cell RNA sequencing, coupled with human spatial RNA transcriptomics) and functional phenotypes, including real-time intravital imaging of murine LPNs versus EANs in inflamed colon revealed LPNs and EANs to have distinct functional identities. LPN programs allowed for heightened motility and pathogen uptake, whereas EANs were overrepresented by hyperactivated/pro-apoptotic states with elevated ROS and inflammatory TNFα production. Thus, we demonstrate that colon LPNs and EANs have distinct functional identities, with EANs exhibiting activated states and apparent cytotoxicity, which may actively contribute to tissue damage. Our findings further identify EANs as potential therapeutic targets for improving mucosal healing and sustaining clinical remission in UC.","PeriodicalId":18877,"journal":{"name":"Mucosal Immunology","volume":" ","pages":""},"PeriodicalIF":7.9,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Female sex hormones and the oral contraceptive pill modulate asthma severity through GLUT-1. 女性性激素和口服避孕药通过 GLUT-1 调节哮喘的严重程度。

IF 7.9 2区医学

Mucosal Immunology Pub Date : 2025-02-26 DOI: 10.1016/j.mucimm.2025.02.006

Alexandra C Brown, Olivia R Carroll, Jemma R Mayall, Nazanin Zounemat-Kermani, Samantha L E Vinzenz, Henry M Gomez, Ed F Mills, Richard Y Kim, Chantal Donovan, Katherine J Baines, Evan J Williams, Bronwyn S Berthon, Katie J Wynne, Hayley A Scott, James W Pinkerton, Yike Guo, Philip M Hansbro, Paul S Foster, Peter A B Wark, Sven-Erik Dahlen, Ian M Adcock, Lisa G Wood, Jay C Horvat

{"title":"Female sex hormones and the oral contraceptive pill modulate asthma severity through GLUT-1.","authors":"Alexandra C Brown, Olivia R Carroll, Jemma R Mayall, Nazanin Zounemat-Kermani, Samantha L E Vinzenz, Henry M Gomez, Ed F Mills, Richard Y Kim, Chantal Donovan, Katherine J Baines, Evan J Williams, Bronwyn S Berthon, Katie J Wynne, Hayley A Scott, James W Pinkerton, Yike Guo, Philip M Hansbro, Paul S Foster, Peter A B Wark, Sven-Erik Dahlen, Ian M Adcock, Lisa G Wood, Jay C Horvat","doi":"10.1016/j.mucimm.2025.02.006","DOIUrl":"https://doi.org/10.1016/j.mucimm.2025.02.006","url":null,"abstract":"Females are disproportionately affected by asthma. An increased understanding of how female sex hormones influence key pathophysiological processes that underpin asthma may identify new, more effective asthma therapies, particularly for females with severe, poorly controlled asthma. We assessed the effects of oral ethinylestradiol/levonorgestrel (representing OCP use) and depot-medroxyprogesterone acetate (DMPA) and estradiol injections on key features of experimental asthma, and determined their effects on glucose transporter-1 (GLUT-1). The effects of OCP use on clinical asthma outcomes, and the relationships between estrogen receptors and type 2 (T2), non-T2, and GLUT-1 responses, in clinical asthma were also determined. OCP and DMPA reduce T2 responses, disease features, and lung expression of GLUT-1, whereas estradiol increases lung expression of GLUT-1, and results in severe, corticosteroid-insensitive, neutrophil-enriched disease, in experimental asthma. OCP use is associated with reduced T2 cytokine and GLUT-1 responses in clinical asthma. GLUT-1 expression is increased in sputum of severe asthmatics, and positively correlates with estrogen receptor expression and both T2 and non-T2 inflammatory responses. Significantly, OCP or GLUT-1 inhibition protects against obesity-associated or estradiol-induced, severe, experimental asthma, respectively. Together, these data show how female sex hormones and the OCP likely modulate asthma severity by modifying GLUT-1 responses in the airways.","PeriodicalId":18877,"journal":{"name":"Mucosal Immunology","volume":" ","pages":""},"PeriodicalIF":7.9,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased ocular plasma cells induce damaging α-synuclein⁺ microglia in autoimmune uveitis.

IF 7.9 2区医学

Mucosal Immunology Pub Date : 2025-02-25 DOI: 10.1016/j.mucimm.2025.02.007

Minghao Li, Meng Feng, Tingting Liu, Songqi Duan, Xuejing Man, Xiaomeng Yuan, Lijie Wang, Yu Sun, Xunbin Wei, Qiang Fu, Baofa Sun, Wei Lin

{"title":"Increased ocular plasma cells induce damaging α-synuclein+ microglia in autoimmune uveitis.","authors":"Minghao Li, Meng Feng, Tingting Liu, Songqi Duan, Xuejing Man, Xiaomeng Yuan, Lijie Wang, Yu Sun, Xunbin Wei, Qiang Fu, Baofa Sun, Wei Lin","doi":"10.1016/j.mucimm.2025.02.007","DOIUrl":"10.1016/j.mucimm.2025.02.007","url":null,"abstract":"Autoimmune uveitis (AIU) is an immune-inflammatory disease that can lead to blindness. However, incomplete understanding of the involved immune cell subsets and their contributions to retinal injury has hindered the development of effective AIU therapies. Using single-cell RNA sequencing and immunofluorescence, we identified α-synuclein+ microglia as the primary subset of damaged ocular cells in the eyes of the experimental autoimmune uveitis (EAU) mouse model. Ocular-infiltrating plasma cells (PCs) were shown to express multiple inflammatory factors, particularly TNF-α, which promoted the production of α-synuclein+ microglia. Studies of heterogeneous PC subtypes revealed that MUC1- PCs represent the primary pathogenic subset, secreting multiple cytokines. Although MUC1+ PCs expressed TGF-β, they exhibited long-lived characteristics and secreted IgG and IgM, thereby prolonging disease progression. Finally, the small G protein Rab1A, also expressed in the PCs of Vogt-Koyanagi-Harada (VKH) patients, was found to mediate autophagy and NF-κB expression, influencing PCs survival and inflammatory responses. Silencing or knocking down Rab1A in PCs inhibited their survival. This study elucidates potential mechanisms underlying the neuroimmune inflammatory response and highlights the previously unrecognized role of infiltrating PCs in AIU, offering novel therapeutic targets for this disease.","PeriodicalId":18877,"journal":{"name":"Mucosal Immunology","volume":" ","pages":""},"PeriodicalIF":7.9,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interleukin-10 production by innate lymphoid cells restricts intestinal inflammation in mice.

IF 7.9 2区医学

Mucosal Immunology Pub Date : 2025-02-21 DOI: 10.1016/j.mucimm.2025.02.005

Jing Li, Justin Jacobse, Jennifer M Pilat, Harsimran Kaur, Weihong Gu, Seung Woo Kang, Mark Rusznak, Hsin-I Huang, Julio Barrera, Pauline A Oloo, Joseph T Roland, Caroline V Hawkins, Andrew P Pahnke, Marian Khalil, M Kay Washington, Keith T Wilson, Christopher S Williams, R Stokes Peebles, Liza Konnikova, Yash A Choksi, Gianna Elena Hammer, Ken S Lau, Jeremy A Goettel

{"title":"Interleukin-10 production by innate lymphoid cells restricts intestinal inflammation in mice.","authors":"Jing Li, Justin Jacobse, Jennifer M Pilat, Harsimran Kaur, Weihong Gu, Seung Woo Kang, Mark Rusznak, Hsin-I Huang, Julio Barrera, Pauline A Oloo, Joseph T Roland, Caroline V Hawkins, Andrew P Pahnke, Marian Khalil, M Kay Washington, Keith T Wilson, Christopher S Williams, R Stokes Peebles, Liza Konnikova, Yash A Choksi, Gianna Elena Hammer, Ken S Lau, Jeremy A Goettel","doi":"10.1016/j.mucimm.2025.02.005","DOIUrl":"10.1016/j.mucimm.2025.02.005","url":null,"abstract":"Interleukin-10 (IL-10) is an immunomodulatory cytokine critical for intestinal immune homeostasis. IL-10 is produced by various immune cells but IL-10 receptor signaling in intestinal CX3CR1+ mononuclear phagocytes is necessary to prevent spontaneous colitis in mice. Here, we utilized fluorescent protein reporters and cell-specific targeting and found that Rorc-expressing innate lymphoid cells (ILCs) produce IL-10 in response to anti-CD40-mediated intestinal inflammation. Deletion of Il10 specifically in Rorc-expressing ILCs led to phenotypic changes in intestinal macrophages and exacerbated both innate and adaptive immune-mediated models of experimental colitis. The population of IL-10+ producing ILCs shared markers with both ILC2 and ILC3 with nearly all ILC3s being of NCR+ subtype. Interestingly, Ccl26 was enriched in IL-10+ ILCs and markedly reduced in IL-10-deficient ILC3s. Since CCL26 is a ligand for CX3CR1, we employed RNA in situ hybridization and observed increased numbers of ILCs in close proximity to Cx3cr1-expressing cells under inflammatory conditions. Finally, we generated a transgenic RorctdTomato reporter mouse that faithfully marked RORγt+ cells that could rescue disease pathology and aberrant macrophage phenotype following adoptive transfer into mice with selective Il10 deficiency in ILC3s. These results demonstrate that IL-10 production by a population of ILCs functions to promote immune homeostasis in the intestine possibly via direct effects on intestinal macrophages.","PeriodicalId":18877,"journal":{"name":"Mucosal Immunology","volume":" ","pages":""},"PeriodicalIF":7.9,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Triggering mouth-resident antiviral CD8⁺ T cells potentiates experimental periodontitis.

IF 7.9 2区医学

Mucosal Immunology Pub Date : 2025-02-21 DOI: 10.1016/j.mucimm.2025.02.003

Flávia M Saavedra, Danielle B Brotto, Vineet Joag, Courtney A Matson, Pavel P Nesmiyanov, Mark C Herzberg, Vaiva Vezys, David Masopust, J Michael Stolley

{"title":"Triggering mouth-resident antiviral CD8+ T cells potentiates experimental periodontitis.","authors":"Flávia M Saavedra, Danielle B Brotto, Vineet Joag, Courtney A Matson, Pavel P Nesmiyanov, Mark C Herzberg, Vaiva Vezys, David Masopust, J Michael Stolley","doi":"10.1016/j.mucimm.2025.02.003","DOIUrl":"10.1016/j.mucimm.2025.02.003","url":null,"abstract":"Emerging evidence indicates that gingival-resident helper CD4+ T cells are major drivers of periodontal inflammation in response to commensal and pathogenic oral microorganisms. Whether tissue-resident memory CD8+ T cells (TRM), which principally safeguard against viruses and cancer but also drive certain autoimmune and inflammatory conditions, impact periodontitis progression and severity remain unknown. We asked whether local reactivation of oral CD8+ TRM of a defined antigen specificity could exacerbate ligature-induced periodontitis (LIP), a well-established model of periodontal disease in mice. Topical application of virus-mimicking peptides to the oral mucosa concurrent with LIP 1) intensified alveolar bone loss, 2) amplified gingival and cervical lymph node inflammation, and 3) stimulated gingival transcriptional changes in genes related to innate immune sensing and cell-mediated cytotoxicity. Therapeutic depletion of CD103-expressing oral CD8+ TRM in advance of LIP prevented exacerbation of disease. These observations provide evidence that oral CD103+ CD8+ TRM have the potential to participate in gingival inflammation, alveolar bone loss, and periodontitis.","PeriodicalId":18877,"journal":{"name":"Mucosal Immunology","volume":" ","pages":""},"PeriodicalIF":7.9,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lung-resident memory Th2 cells regulate pulmonary cryptococcosis by inducing type-II granuloma formation.

IF 7.9 2区医学

Mucosal Immunology Pub Date : 2025-02-19 DOI: 10.1016/j.mucimm.2025.02.004

Keigo Ueno, Akiko Nagamori, Nahoko Oniyama Honkyu, Kyung J Kwon-Chung, Yoshitsugu Miyazaki

{"title":"Lung-resident memory Th2 cells regulate pulmonary cryptococcosis by inducing type-II granuloma formation.","authors":"Keigo Ueno, Akiko Nagamori, Nahoko Oniyama Honkyu, Kyung J Kwon-Chung, Yoshitsugu Miyazaki","doi":"10.1016/j.mucimm.2025.02.004","DOIUrl":"10.1016/j.mucimm.2025.02.004","url":null,"abstract":"Lung-resident memory T cells (lung TRMs) settle in the lung and respond rapidly to external antigens, and are therefore considered to have great potential for development of respiratory vaccines. Here, we demonstrate that lung-resident memory Th2 cells (lung TRM2) protect against pulmonary mycosis caused by Cryptococcus gattii. We developed novel whole-cell intranasal vaccines using a heat-inactivated C.gattii capsule-deficient strain cap59Δ, which induced ST-2+ Gata-3+ lung TRM2 specifically responding to C.gattii whole-cell antigen. Lung fungal burden and survival rate were significantly improved in immunized mice after infection challenge. The immunosuppressive agent FTY720 did not impact vaccine effectiveness, and adoptive transfer of lung TRMs into Rag-1-deficient mice decreased the lung fungal burden. In IL-4/IL-13 double-knockout (DKO) mice, immunization did not efficiently induce eosinophil recruitment and granuloma formation, and the fungal burden was not decreased after infection challenge. Co-culture of lung TRM2 with myeloid lineages induced multinucleated giant cells (MGCs) in the presence of antigen, which phagocytosed live C.gattii cells without opsonization, whereas lung TRM2 from DKO mice did not induce MGCs. These findings provide a new model in which lung TRM2 suppress C.gattii infection via granuloma induction.","PeriodicalId":18877,"journal":{"name":"Mucosal Immunology","volume":" ","pages":""},"PeriodicalIF":7.9,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influenza virus-induced type I interferons disrupt alveolar epithelial repair and tight junction integrity in the developing lung.

IF 7.9 2区医学

Mucosal Immunology Pub Date : 2025-02-19 DOI: 10.1016/j.mucimm.2025.02.002

Abigail P Onufer, Joshua Chang Mell, Laura Cort, Abhishek Rao, Nontokozo V Mdluli, Alison J Carey

{"title":"Influenza virus-induced type I interferons disrupt alveolar epithelial repair and tight junction integrity in the developing lung.","authors":"Abigail P Onufer, Joshua Chang Mell, Laura Cort, Abhishek Rao, Nontokozo V Mdluli, Alison J Carey","doi":"10.1016/j.mucimm.2025.02.002","DOIUrl":"10.1016/j.mucimm.2025.02.002","url":null,"abstract":"Recently, we demonstrated that influenza A virus (IAV)-infected murine neonates lacking a functional IFN-I receptor (IFNAR-/-) had significantly improved survival and reduced lung pathology relative to wild-type (WT) neonates. In direct contrast, adult IFNAR-/- mice display enhanced morbidity following IAV infection relative to WT adults. We hypothesized that IAV-induced IFN-I signaling in primary neonatal type II alveolar epithelial cells (TIIECs), the main cell type of IAV infection and initiator of host response in the lung, contributed to age-specific viral pathogenesis. Multifactorial transcriptional analysis of purified TIIECs revealed age, not infection status, as the primary driver of transcriptional differences in TIIECs. Subsequent pathway analysis demonstrated IAV-infected IFNAR-/- neonates significantly upregulated cell proliferation, tissue repair and tight junction genes at 2-days post-infection (dpi), compared to WT neonates. Next, to determine if these growth and repair differences persisted later in infection, targeted analysis of repair gene expression and immunofluorescent quantification of pulmonary sealing tight junction molecules ZO-1 and occludin was performed at 6-dpi. Relative to WT neonates, IFNAR-/- neonates had significantly higher whole lung occludin staining and repair gene expression. Together, our data demonstrates IFN-I signaling is extremely pathogenic in the developing lung by disrupting alveolar repair and pulmonary barrier integrity.","PeriodicalId":18877,"journal":{"name":"Mucosal Immunology","volume":" ","pages":""},"PeriodicalIF":7.9,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143472767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IL-6 mediates defense against influenza virus by promoting protective antibody responses but not innate inflammation. IL-6 通过促进保护性抗体反应而非先天性炎症，介导对流感病毒的防御。

IF 7.9 2区医学

Mucosal Immunology Pub Date : 2025-02-18 DOI: 10.1016/j.mucimm.2025.02.001

F Piattini, N D Sidiropoulos, I Berest, M Kopf

{"title":"IL-6 mediates defense against influenza virus by promoting protective antibody responses but not innate inflammation.","authors":"F Piattini, N D Sidiropoulos, I Berest, M Kopf","doi":"10.1016/j.mucimm.2025.02.001","DOIUrl":"10.1016/j.mucimm.2025.02.001","url":null,"abstract":"Influenza virus infection is a leading cause of morbidity and mortality worldwide, posing a significant public health problem. The pro-inflammatory cytokine interleukin-6 (IL-6) has been shown to promote defense against respiratory viral infection, while excessive IL-6 responses have been associated with severe pneumonia. Heterogenous expression of IL-6R and the IL-6-signal transducer subunit (gp130) across many cell types and different signaling modalities have made it difficult to define the precise role of the IL-6/IL-6R pathway in vivo. We generated multiple cell lineage-specific Il6ra-deficient mice and compared them to global Il6ra-/- and Il-6-/- mice to dissect the systemic and cell-intrinsic mechanisms for pneumonitis and control of influenza A virus (IAV) infection. Delayed viral clearance and severe morbidity in the global IL-6 knockouts were associated with reduced antibody responses, complement C3 and C5 production, and impaired T follicular helper (Tfh) cell generation. Mice lacking IL-6R exclusively in T cells phenocopied a defect in Tfh cell differentiation and antibody production, although susceptibility to IAV was only mildly affected. Mice lacking IL-6R specifically in B cells mounted normal antibody responses. Moreover, innate pro-inflammatory cytokine responses, myeloid cell infiltration, and adaptive immunity in the lung remained unaffected in Il6rafl/flLysMCre mice. Our results suggest that IL-6 mediates defense against IAV mainly by generating Tfh cells and promoting local C3 production, which together are required for eliciting protective antibody responses by B cells.","PeriodicalId":18877,"journal":{"name":"Mucosal Immunology","volume":" ","pages":""},"PeriodicalIF":7.9,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibiotic-induced dysbiosis of the ocular microbiome affects corneal circadian rhythmic activity in mice.

IF 7.9 2区医学

Mucosal Immunology Pub Date : 2025-02-05 DOI: 10.1016/j.mucimm.2025.01.010

Xinwei Jiao, Yan Li, Yu Hu, Ruyu Yan, Ting Fu, Jun Liu, Zhijie Li

{"title":"Antibiotic-induced dysbiosis of the ocular microbiome affects corneal circadian rhythmic activity in mice.","authors":"Xinwei Jiao, Yan Li, Yu Hu, Ruyu Yan, Ting Fu, Jun Liu, Zhijie Li","doi":"10.1016/j.mucimm.2025.01.010","DOIUrl":"10.1016/j.mucimm.2025.01.010","url":null,"abstract":"The ocular surface microbiota plays a critical role in maintaining corneal homeostasis, but its disruption and subsequent effects on corneal functions remain poorly understood. This study investigates how antibiotic-induced microbial depletion affects the corneal circadian transcriptome in C57BL/6J mice. Dysbiosis was induced using a topical antibiotic cocktail, and RNA sequencing was employed to analyze gene expression across eight time points over 24 h. Antibiotic treatment disrupted corneal circadian rhythms, eliminating rhythmicity in 1,812 genes and introducing rhythmicity in 1,928 previously arrhythmic genes. Furthermore, epithelial adhesion was impaired, inflammation was elevated, and neural sensitivity was reduced. More than 50 % of ocular microbial genera exhibited daily oscillations, with six genera showing significant correlations with corneal rhythmic transcripts. Additionally, the administration of TLR agonists restored circadian gene expression patterns, with partial recovery of corneal barrier function and immune homeostasis, further highlighting the potential of microbiota-targeted therapies in treating ocular surface disorders. These findings underscore the critical role of the ocular microbiota in regulating corneal health and suggest that restoring microbial balance via TLR activation may offer new therapeutic avenues for eye diseases.","PeriodicalId":18877,"journal":{"name":"Mucosal Immunology","volume":" ","pages":""},"PeriodicalIF":7.9,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial damage-associated molecular patterns: New perspectives for mitochondria and inflammatory bowel diseases.

IF 7.9 2区医学

Mucosal Immunology Pub Date : 2025-02-05 DOI: 10.1016/j.mucimm.2025.01.013

Zhijie Wang, Zixuan He, Xin Chang, Lu Xie, Yihang Song, Haicong Wu, Hao Zhang, Shuling Wang, Xiaofeng Zhang, Yu Bai

引用次数: 0