Mutation Research\/environmental Mutagenesis and Related Subjects最新文献_第2页

The 28th annual environmental mutagen society meeting 第28届环境诱变剂学会年会

Mutation Research\/environmental Mutagenesis and Related Subjects Pub Date : 1996-12-12 DOI: 10.1016/S0165-1161(96)00037-4

引用次数: 0

Chromosome analysis of peripheral lymphocytes from persons exposed to radioactive fallout in Norway from the Chernobyl accident 挪威切尔诺贝利事故放射性沉降物暴露者外周淋巴细胞的染色体分析

Mutation Research\/environmental Mutagenesis and Related Subjects Pub Date : 1996-12-12 DOI: 10.1016/S0165-1161(96)90241-1

A. Brøgger , J.B. Reitan , P. Strand , I. Amundsen

引用次数: 12

Chrysotile asbestos fibers mediate homologous recombination in Rat2λ fibroblasts: implications for carcinogenesis 温石棉纤维介导Rat2λ成纤维细胞的同源重组：致癌作用的意义

Mutation Research\/environmental Mutagenesis and Related Subjects Pub Date : 1996-12-12 DOI: 10.1016/S0165-1161(96)90245-9

Kimberly Lezon-Geyda , Cindy M. Jaime , James H. Godbold , Ernest F. Savransky , Aluko Hope , Samir A. Kheiri , Zlatica M. Dzmura , Hiroshi Uehara , Edward M. Johnson , Thomas M. Fasy

{"title":"Chrysotile asbestos fibers mediate homologous recombination in Rat2λ fibroblasts: implications for carcinogenesis","authors":"Kimberly Lezon-Geyda , Cindy M. Jaime , James H. Godbold , Ernest F. Savransky , Aluko Hope , Samir A. Kheiri , Zlatica M. Dzmura , Hiroshi Uehara , Edward M. Johnson , Thomas M. Fasy","doi":"10.1016/S0165-1161(96)90245-9","DOIUrl":"https://doi.org/10.1016/S0165-1161(96)90245-9","url":null,"abstract":"<div>Asbestos fibers are widespread environmental carcinogens whose mutagenicity is now established. Nonetheless, the molecular nature of these mutations and the mechanisms by which they accelerate carcinogenesis remain poorly understood. We have assessed the ability of asbestos fibers to promote homologous recombination, a potent mechanism for generating intrachromosomal rearrangements, such as deletions, and mitotic recombination. For this, we have developed a new assay which determines the extent to which a marker gene present in DNA introduced by asbestos can recombine with homologous genes residing in a transfected cell. We have demonstrated that Calidria chrysotile fibers are mutagenic and are able to mediate transfection of molecularly marked mutant lacI genes in a manner that results in their preferential recombination with homologous wild-type genes in the transfected cell. Asbestos induced recombination events may play a significant role in asbestos mutagenesis and carcinogenesis, and promotion of recombination may underlie the well-recognized synergy of asbestos with other carcinogens.</div>","PeriodicalId":18870,"journal":{"name":"Mutation Research\\/environmental Mutagenesis and Related Subjects","volume":"361 2","pages":"Pages 113-120"},"PeriodicalIF":0.0,"publicationDate":"1996-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1161(96)90245-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72050018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Large deletion detected with the Big Blue® transgenic mouse mutagenesis assay 用Big Blue®转基因小鼠诱变试验检测到大缺失

Mutation Research\/environmental Mutagenesis and Related Subjects Pub Date : 1996-12-12 DOI: 10.1016/S0165-1161(96)00032-5

Victoria L. Buettner , Hiroshi Nishino , Steve S. Sommer

引用次数: 9

A plasmid rescue to investigate mutagenesis in transgenic D. melanogaster 用质粒拯救法研究转基因黑腹田鼠的诱变作用

Mutation Research\/environmental Mutagenesis and Related Subjects Pub Date : 1996-12-12 DOI: 10.1016/S0165-1161(96)90251-4

Martin Hersberger , Kim Kirby , John P. Phillips , Friedrich E. Würgler , Theo Koller , Rosa M. Widmer

{"title":"A plasmid rescue to investigate mutagenesis in transgenic D. melanogaster","authors":"Martin Hersberger , Kim Kirby , John P. Phillips , Friedrich E. Würgler , Theo Koller , Rosa M. Widmer","doi":"10.1016/S0165-1161(96)90251-4","DOIUrl":"10.1016/S0165-1161(96)90251-4","url":null,"abstract":"<div>We present a plasmid rescue from transgenic Drosophila to study spontaneous and mutagen-induced mutations in vivo. Transgenic Drosophila lines were established by tranformation with a shuttle vector containing the bacterial lacZ gene as a target for mutagenesis. The target gene cen be recovered into bacteria by restriction endonuclease treatment of total genomic DNA, followed by ligation on the recircularized shuttle vectors. The resulting circular plasmids are then transformed back into E. coli lacZ− mutants, where the activity of the lacZ genes is scored on the induction substrate X-Gal. The number of inactivated versus intact lacZ genes directly indicates the mutation frequency. By the described target gene rescue procedure up to 5000 lacZ gene copies can be rescued from one fly routinely. Spontaneous background mutation rates using this system are 2.6±0.6×10−4. Treatment of larvae with ethylnitrosourea (ENU) resulted in a dose-dependent increase of the mutation frequency to 4.8±0.6×10−4 for 0.5 mM and 6.9±1.2×10−4 for 1 mM ENU, respectively.</div>","PeriodicalId":18870,"journal":{"name":"Mutation Research\\/environmental Mutagenesis and Related Subjects","volume":"361 2","pages":"Pages 165-172"},"PeriodicalIF":0.0,"publicationDate":"1996-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1161(96)90251-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19942266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Antimutagens from Plumeria acuminata Ait 尖羽藻中的抗突变物质。

Mutation Research\/environmental Mutagenesis and Related Subjects Pub Date : 1996-12-12 DOI: 10.1016/S0165-1161(96)90240-X

Amelia P. Guevara , Evangeline Amor , Graeme Russell

{"title":"Antimutagens from Plumeria acuminata Ait","authors":"Amelia P. Guevara , Evangeline Amor , Graeme Russell","doi":"10.1016/S0165-1161(96)90240-X","DOIUrl":"10.1016/S0165-1161(96)90240-X","url":null,"abstract":"<div>Four isolates, A1, C1, D3, and F2, from the ethanol extract of the green leaves of Plumeria acuminata Ait. showed antimutagenic activity. The antimutagens were isolated from he bioactive hexane and carbon tetrachloride fractionsFour isolates, A1, C1, D3, and F2, from the ethanol extract of the green leaves of Plumeria acuminata Ait. showed antimutagenic activity. The antimutagens were isolated from the bioactive hexane and carbon tetrachloride fractions following a bioactivity-directed fractionation scheme and using the micronucleus test to monitor the antimutagenic activities. Structure elucidation studies indicated that C1 is stigmast-7-enol [1], D3 is lupeol carboxylic acid [2] and F2 is ursolic acid [3]. The structure of A1 was not fully elucidated but MS data suggested that it contained a long hydrocarbon chain. At a dosage of 2 mg isolate/25 g mouse, A1 reduced the number of micronucleated polychromatic erythrocytes (MPCE) induced by the mutagen, mitomycin C, by 75%, C1 by 80%, D3 by 57%, and F2 by 76%. Compound A2 was also isolated but was found inactive. Its structure was identified to be lupeol acetate [4].</div>","PeriodicalId":18870,"journal":{"name":"Mutation Research\\/environmental Mutagenesis and Related Subjects","volume":"361 2","pages":"Pages 67-72"},"PeriodicalIF":0.0,"publicationDate":"1996-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1161(96)90240-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19942286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

Contents volume 361 (1996) 目录第361卷（1996）

Mutation Research\/environmental Mutagenesis and Related Subjects Pub Date : 1996-12-12 DOI: 10.1016/S0165-1161(96)90253-8

引用次数: 0

A new possible parameter for the detection of aneuploidy inducing substances: the analysis of qualitative and quantitative abnormalities of the spindle apparatus 检测非整倍体诱导物质的一种新的可能参数:纺锤体的定性和定量异常分析

Mutation Research\/environmental Mutagenesis and Related Subjects Pub Date : 1996-12-12 DOI: 10.1016/S0165-1161(96)90239-3

U. Kochendörfer , I. Stammberger , D. Mayer , G. Schwanitz

{"title":"A new possible parameter for the detection of aneuploidy inducing substances: the analysis of qualitative and quantitative abnormalities of the spindle apparatus","authors":"U. Kochendörfer , I. Stammberger , D. Mayer , G. Schwanitz","doi":"10.1016/S0165-1161(96)90239-3","DOIUrl":"10.1016/S0165-1161(96)90239-3","url":null,"abstract":"<div>In the present study, compared to other cytogenetic methods, we measured the number of aneuploid cells directly by analyzing anomalies of the mitotic spindle. Qualitative and quantitative abnormalities of the mitotic spindle apparatus in transformed and non-transformed cell lines in vitro were classified. We treated the different cell lines with well known aneugenic agents as Benomyl and Griseofulvin and investigated the mitotic spindle under different experimental conditions. The spindle apparatus was stained by indirect immunofluorescence and the chromatin was counterstained by fluorescent dyes. The mitotic spindle showed a great sensitivity to the aneuploidy-inducing substances used in our experiments. The spindle-disturbing effect of the tested substances was demonstrated to be dose-dependent. The morphological alterations appeared to be independent of the aneuploidy-inducing test substance used, but showed a relation to the dose and length of treatment. Thus, the analysis of the mitotic spindle may be a useful screening parameter for the detection of aneuploidy-inducing substances and further investigations will provide additional results to specific parameters.</div>","PeriodicalId":18870,"journal":{"name":"Mutation Research\\/environmental Mutagenesis and Related Subjects","volume":"361 2","pages":"Pages 55-66"},"PeriodicalIF":0.0,"publicationDate":"1996-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1161(96)90239-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19942285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Induction of sister chromatid exchange by 3,4-epoxybutane- 1,2-diol in cultured human lymphocytes of different GSTT1 and GSTM1 genotypes 3,4-环氧丁烷-1,2-二醇对不同GSTT1和GSTM1基因型人淋巴细胞姊妹染色单体交换的诱导作用

Mutation Research\/environmental Mutagenesis and Related Subjects Pub Date : 1996-12-12 DOI: 10.1016/S0165-1161(96)90246-0

Sabrina Bernardini , Katarina Pelin , Kimmo Peltonen , Hilkka Järventaus , Ari Hirvonen , Constantin Neagu , Marja Sorsa , Hannu Norppa

{"title":"Induction of sister chromatid exchange by 3,4-epoxybutane- 1,2-diol in cultured human lymphocytes of different GSTT1 and GSTM1 genotypes","authors":"Sabrina Bernardini , Katarina Pelin , Kimmo Peltonen , Hilkka Järventaus , Ari Hirvonen , Constantin Neagu , Marja Sorsa , Hannu Norppa","doi":"10.1016/S0165-1161(96)90246-0","DOIUrl":"https://doi.org/10.1016/S0165-1161(96)90246-0","url":null,"abstract":"<div>The induction of sister chromatid exchanges (SCEs) by a 48-h treatment with 3,4-epoxybutane-1,2-diol (EBD), a metabolite of 1,3-butadiene, was studied in whole-blood lymphocyte cultures of 22 human donors with known genotypes of two polymorphic glutathione S-transferases (GSTs), GSTT1 and GSTM1. For both genes, donors representing a homozygous ‘null’ genotype lacking the respective GST gene and isozyme and a ‘positive’ genotype with at least one intact gene and GST activity were included. The mean frequencies of SCE/cell were similar in all genotype groups: GSTT1 null (n = 10) (mean 22.0 for 250 μM and 32.9 for 250 500 μM of EBD), GSTT1 positive (n = 14) (21.3 and 34.6, respectively), GSTM1 null (n = 10) (20.3 and 33.5) and GSTM1 positive donors (n = 15) (20.6 and 34.8). At 500 μM concentration of EBD, the lymphocyte cultures of all donors showed a significantly decreased replication index. No differences in EDB-induced SCEs or in replication index could be associated with the GSTM1 and GSTT1 genotypes either separately or in combination. When SCEs induction by EBD was compared to that of two other known epoxide metabolites of butadiene, 1,2:3,4-diepoxybutane (DEB) was effective at concentrations over two orders of magnitude lower than EBD or 1,2-epoxy-3-butene (MEB). It is concluded that EBD is an efficient inducer of SCE in cultured human lymphocytes, although not quite as effective as MEB and clearly less effective than DEB. Contrary to previous findings with DEB and MEB, the polymorphic GSTM1 and GSTT1 do not appear to be involved in the detoxification of EBD in human lymphocytes.</div>","PeriodicalId":18870,"journal":{"name":"Mutation Research\\/environmental Mutagenesis and Related Subjects","volume":"361 2","pages":"Pages 121-127"},"PeriodicalIF":0.0,"publicationDate":"1996-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1161(96)90246-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72050019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Comparison of spontaneous structural chromosome aberration frequency in 48 h-cultured human lymphocytes mitotically arrested by different colcemid treatments 不同秋水仙药处理有丝分裂阻滞48 h培养人淋巴细胞自发结构染色体畸变频率的比较

Mutation Research\/environmental Mutagenesis and Related Subjects Pub Date : 1996-09-26 DOI: 10.1016/S0165-1161(96)90227-7

Roberto Scarpato, Lucia Migliore

{"title":"Comparison of spontaneous structural chromosome aberration frequency in 48 h-cultured human lymphocytes mitotically arrested by different colcemid treatments","authors":"Roberto Scarpato, Lucia Migliore","doi":"10.1016/S0165-1161(96)90227-7","DOIUrl":"10.1016/S0165-1161(96)90227-7","url":null,"abstract":"<div>Alterations in mitotic index and cell cycle kinetics are reported to be dependent on both the culture conditions and the ability of the lymphocytes of each individual to respond to phytohaemagglutinin stimulus. Thus, the frequency of structural chromosome aberrations (CA) could prove to be affected to some degree by these parameters. CA frequency and cell proliferation index (PI) were assessed in a group of healthy subjects after adding colcemid to cultured lymphocytes 3 h (standard) and 22 h (modified) before cell fixation at 48 h. All control cultures treated with colcemid for 22 h consisted exclusively of first metaphases, whereas the proportion of second-division lymphocytes in standard cultures (3 h colcemid) ranged from 4% to 49%. In addition, CA frequencies with and without gaps were always elevated in modified cultures as compared to the standard ones, and the difference between CA percentages obtained with the two methods was found to be significantly related with increasing PI values.</div>","PeriodicalId":18870,"journal":{"name":"Mutation Research\\/environmental Mutagenesis and Related Subjects","volume":"361 1","pages":"Pages 35-39"},"PeriodicalIF":0.0,"publicationDate":"1996-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1161(96)90227-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19784966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15