A new possible parameter for the detection of aneuploidy inducing substances: the analysis of qualitative and quantitative abnormalities of the spindle apparatus

Mutation Research\/environmental Mutagenesis and Related Subjects Pub Date : 1996-12-12 DOI:10.1016/S0165-1161(96)90239-3

U. Kochendörfer , I. Stammberger , D. Mayer , G. Schwanitz

{"title":"A new possible parameter for the detection of aneuploidy inducing substances: the analysis of qualitative and quantitative abnormalities of the spindle apparatus","authors":"U. Kochendörfer , I. Stammberger , D. Mayer , G. Schwanitz","doi":"10.1016/S0165-1161(96)90239-3","DOIUrl":null,"url":null,"abstract":"<div><p>In the present study, compared to other cytogenetic methods, we measured the number of aneuploid cells directly by analyzing anomalies of the mitotic spindle. Qualitative and quantitative abnormalities of the mitotic spindle apparatus in transformed and non-transformed cell lines in vitro were classified. We treated the different cell lines with well known aneugenic agents as Benomyl and Griseofulvin and investigated the mitotic spindle under different experimental conditions. The spindle apparatus was stained by indirect immunofluorescence and the chromatin was counterstained by fluorescent dyes. The mitotic spindle showed a great sensitivity to the aneuploidy-inducing substances used in our experiments. The spindle-disturbing effect of the tested substances was demonstrated to be dose-dependent. The morphological alterations appeared to be independent of the aneuploidy-inducing test substance used, but showed a relation to the dose and length of treatment. Thus, the analysis of the mitotic spindle may be a useful screening parameter for the detection of aneuploidy-inducing substances and further investigations will provide additional results to specific parameters.</p></div>","PeriodicalId":18870,"journal":{"name":"Mutation Research\\/environmental Mutagenesis and Related Subjects","volume":"361 2","pages":"Pages 55-66"},"PeriodicalIF":0.0000,"publicationDate":"1996-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0165-1161(96)90239-3","citationCount":"11","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mutation Research\\/environmental Mutagenesis and Related Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165116196902393","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 11

Abstract

In the present study, compared to other cytogenetic methods, we measured the number of aneuploid cells directly by analyzing anomalies of the mitotic spindle. Qualitative and quantitative abnormalities of the mitotic spindle apparatus in transformed and non-transformed cell lines in vitro were classified. We treated the different cell lines with well known aneugenic agents as Benomyl and Griseofulvin and investigated the mitotic spindle under different experimental conditions. The spindle apparatus was stained by indirect immunofluorescence and the chromatin was counterstained by fluorescent dyes. The mitotic spindle showed a great sensitivity to the aneuploidy-inducing substances used in our experiments. The spindle-disturbing effect of the tested substances was demonstrated to be dose-dependent. The morphological alterations appeared to be independent of the aneuploidy-inducing test substance used, but showed a relation to the dose and length of treatment. Thus, the analysis of the mitotic spindle may be a useful screening parameter for the detection of aneuploidy-inducing substances and further investigations will provide additional results to specific parameters.

查看原文本刊更多论文

检测非整倍体诱导物质的一种新的可能参数:纺锤体的定性和定量异常分析

在本研究中，与其他细胞遗传学方法相比，我们通过分析有丝分裂纺锤体的异常来直接测量非整倍体细胞的数量。对转化细胞系和非转化细胞系有丝分裂纺锤体的定性和定量异常进行了分类。在不同的实验条件下，我们用著名的非优生药物如苯丙胺和灰黄霉素处理不同的细胞系，并研究了有丝分裂纺锤体。纺锤体用间接免疫荧光染色，染色质用荧光染料反染。有丝分裂纺锤体对实验中使用的非整倍体诱导物质表现出极大的敏感性。所测物质的纺锤体干扰效应呈剂量依赖性。形态改变似乎与使用的非整倍体诱导试验物质无关，但与处理剂量和时间有关。因此，有丝分裂纺锤体的分析可能是检测非整倍体诱导物质的有用筛选参数，进一步的研究将为特定参数提供额外的结果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

$Mutation Research\/environmental Mutagenesis and Related Subjects$

Mutation Research\/environmental Mutagenesis and Related Subjects

自引率

0.00%

发文量