MycosciencePub Date : 2023-07-31DOI: 10.47371/mycosci.2023.06.002
T. Kasuya, Reiichiro Kaneko, S. Takehashi, K. Hosaka
{"title":"Hydropodia silvae-nipponicae (Porotheleaceae), a new species from evergreen broad-leaved forests in Japan","authors":"T. Kasuya, Reiichiro Kaneko, S. Takehashi, K. Hosaka","doi":"10.47371/mycosci.2023.06.002","DOIUrl":"https://doi.org/10.47371/mycosci.2023.06.002","url":null,"abstract":"Hydropodia silvae-nipponicae, a new agaric species was described based on the specimens collected from evergreen broad-leaved forests in Japan. The present new species is distinct from H. subalpina, the type species of the genus by morphological characteristics and nuclear rDNA sequences. Hydropodia silvae-nipponicae is characterized by its nodulose cystidium-like terminal elements of pileipellis, cylindrical to clavate pileocystidia with irregular excrescences, and dimorphic pleurocystidia. It is the second species belonging to the genus Hydropodia.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"85 5","pages":""},"PeriodicalIF":1.4,"publicationDate":"2023-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41311573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"<i>Hypochnicium</i> sensu lato (<i>Polyporales</i>, <i>Basidiomycota</i>) from Japan, with descriptions of a new genus and three new species.","authors":"Nitaro Maekawa, Ryo Sugawara, Hiroki Kogi, Shungo Norikura, Kozue Sotome, Naoki Endo, Akira Nakagiri, Shuji Ushijima","doi":"10.47371/mycosci.2022.10.001","DOIUrl":"10.47371/mycosci.2022.10.001","url":null,"abstract":"<p><p>Species of <i>Hypochnicium</i> (<i>Polyporales</i>, <i>Basidiomycota</i>) collected from Japan were studied on their taxonomy by morphological and phylogenetic approaches. Phylogenetic analyses based on a nrDNA LSU and ITS dataset including the Japanese specimens and other publicly available ones show that <i>Hypochnicium</i> is polyphyletic. Since the clade containing the type species <i>H. bombycinum</i> was well-supported, we defined this clade as <i>Hypochnicium</i> s. str., and emended <i>Hypochnicium</i> to include restricted taxa with only smooth basidiospores. The new genus <i>Neohypochnicium</i> is proposed to accommodate the remaining taxa excluded from the genus <i>Hypochnicium</i> s. str., which includes both species with smooth basidiospores and ornamented ones. Three new species, <i>Gyrophanopsis japonica</i>, <i>N. asiaticum</i> and <i>N. perlongicystidiosum</i> are described and illustrated based on morphological and phylogenetic analyses using an ITS region dataset. In addition, the following 15 new combinations are proposed: <i>N. albostramineum</i>, <i>N. aotearoae</i>, <i>N. capitulateum</i>, <i>N. cremicolor</i>, <i>N. cystidiatum</i>, <i>N. geogenium</i>, <i>N. guineense</i>, <i>N. huinayense</i>, <i>N. michelii</i>, <i>N. microsporum</i>, <i>N. patagonicum</i>, <i>N. pini</i>, <i>N. punctulatum</i>, <i>N. subrigescens</i> and <i>N. wakefieldiae</i>. An identification key to Japanese species of <i>Bulbillomyces</i>, <i>Gyrophanopsis</i>, <i>Hypochnicium</i> and <i>Neohypochnicium</i> is provided.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"64 1","pages":"19-34"},"PeriodicalIF":1.5,"publicationDate":"2023-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c1/3f/MYC-64-019.PMC10042306.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9443822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycosciencePub Date : 2023-01-01DOI: 10.47371/mycosci.2023.09.003
Tie-Zhi Liu, Li Liu, Jing Wen, Shu-Yan Liu
{"title":"<i>Neoerysiphe leontopodii sp.</i> nov., a new powdery mildew from China","authors":"Tie-Zhi Liu, Li Liu, Jing Wen, Shu-Yan Liu","doi":"10.47371/mycosci.2023.09.003","DOIUrl":"https://doi.org/10.47371/mycosci.2023.09.003","url":null,"abstract":"A powdery mildew was found on Leontopodium leontopodioides (Asteraceae) in China. Phylogenetic analyses using a combination of internal transcribed spacer and 28S rDNA sequences showed that this species, which clusters as sister to Neoerysiphe joerstadii, is allied to N. galii, N. geranii, and N. nevoi. This species differs from the closely allied N. joerstadii in the number and size of asci (3–10 asci, 55–75 × 20–40 μm versus 16–32 asci, 40–60 × 20–30 μm). This species is morphologically very similar to N. gnaphalii, but clearly differs from this species in having larger chasmothecia and colorless appendages. Therefore, the powdery mildew on L. leontopodioides is described as N. leontopodii sp. nov.","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"132 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135267801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Characterization of <i>Penicillium</i> Species Isolated from <i>Dioscorea polystachya</i> in Hokkaido, Japan.","authors":"Roselyn Jove Uy, Miyuki Kayamori, Chiharu Nakashima","doi":"10.47371/mycosci.2022.11.002","DOIUrl":"https://doi.org/10.47371/mycosci.2022.11.002","url":null,"abstract":"<p><p>Chinese yam (<i>Dioscorea polystachya</i>) has been cultivated as a food crop in Japan. The blue mold disease of Chinese yam during the storage and treatment of forced sprouting is one of the hindrances in attaining productivity that can lead to food security in tropical countries and steady farming that will eventually lead to farmers' profitability. In this study, three species of the genus <i>Penicillium</i> related to the blue mold of yam were isolated from two locations in Hokkaido, Japan. From the morphology and phylogeny, these were <i>Penicillium albocoremium</i>, <i>P. polonicum</i>, and <i>P. sclerotigenum</i>. Pathogenicity was confirmed in all <i>Penicillium</i>-like isolates, and sensitivity against benomyl was evaluated based on MIC. The first two species were newly recorded as the causal fungi of the blue mold of yam in Japan, and the pathogenicity of <i>P. albocoremium</i> is first confirmed in yam seed tuber. Moreover, some isolates showed possible resistance to benomyl.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"64 1","pages":"11-18"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ce/5c/MYC-64-011.PMC10025074.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9741089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycosciencePub Date : 2023-01-01DOI: 10.47371/mycosci.2023.01.001
Ryota Kusakabe, Masahide Yamato
{"title":"Isolation and identification of an arbuscular mycorrhizal fungus specifically associated with mycoheterotrophic seedlings of <i>Gentiana zollingeri</i> (<i>Gentianaceae</i>).","authors":"Ryota Kusakabe, Masahide Yamato","doi":"10.47371/mycosci.2023.01.001","DOIUrl":"https://doi.org/10.47371/mycosci.2023.01.001","url":null,"abstract":"<p><p><i>Gentiana zollingeri</i> (<i>Gentianaceae</i>) is an initial mycoheterotrophic plant that depends on a specific group of arbuscular mycorrhizal (AM) fungi for carbon source during underground growth after seed germination. In this study, a mycorrhizal fungus dominant in mycoheterotrophic seedlings of <i>G. zollingeri</i> was successfully isolated from a soil core collected from a point close to a flowering <i>G. zollingeri</i>. The AM fungal isolate was identified as conspecific or closely related to <i>Dominikia aurea</i> (<i>Glomeraceae</i>) by spore morphology and molecular phylogeny. Basic Local Alignment Search Tool (BLAST) searches against the Maarj<i>AM</i> database showed that the nuclear small subunit ribosomal DNA sequences of the isolate matched the AM fungal sequences obtained from a wide range of plants in various ecosystems, including several mycoheterotrophs. Thus, it is suggested that the AM fungal isolate is one of the cheating susceptible AM fungi. Furthermore, the sequences corresponded to those of a group of AM fungi dominantly detected in Japanese temperate forests. Accordingly, there is a possibility that mycoheterotrophic plants, including seedlings of <i>G. zollingeri</i>, may target AM fungi with a wide host range and ubiquitous distribution.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"64 2","pages":"55-62"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d2/43/MYC-64-055.PMC10165169.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9446096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Re-examination of a rare protosteloid amoeba <i>Schizoplasmodiopsis micropunctata</i>, and the revision of <i>Tychosporium</i> (<i>Cavosteliida</i>, <i>Variosea</i>, <i>Amoebozoa</i>).","authors":"Yoshiaki Iwamoto, Yousuke Degawa, Takeshi Nakayama","doi":"10.47371/mycosci.2023.01.002","DOIUrl":"https://doi.org/10.47371/mycosci.2023.01.002","url":null,"abstract":"<p><p>The genus <i>Schizoplasmodiopsis</i> is one of the most morphologically diverse groups among the class <i>Variosea</i>. Recent phylogenetic studies suggest that <i>Schizoplasmodiopsis</i> is polyphyletic, but there are few taxonomic studies of this genus. We established <i>S. micropunctata</i> strain YIP-40, observed in detail its of morphology and lifecycle, and conducted a phylogenetic analysis. The phylogenetic analysis revealed that <i>S. micropunctata</i> was sister to <i>Tychosporium acutostipes</i>. Scanning electron microscopy showed <i>S. micropunctata</i> had a non-deciduous hilum structure that is unique to <i>Tychosporium</i>. The morphology of amoebae, mitotic behavior, and prespore cells of <i>S. micropunctata</i> also supported the close relationship to <i>Tychosporium</i>. We propose to transfer <i>S. micropunctata</i> to <i>Tychosporium</i> and emend the generic concept of <i>Tychosporium</i> to include this species.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"64 2","pages":"63-68"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/69/7b/MYC-64-063.PMC10165171.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9452437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycosciencePub Date : 2023-01-01DOI: 10.47371/mycosci.2023.03.001
Ya-Li Sun, Tolgor Bau
{"title":"<i>Rhodocybe subasyae</i>, a new species of <i>Rhodocybe</i> sect. <i>Rufobrunnea</i> (<i>Entolomataceae, Agaricales</i>) from northeast China.","authors":"Ya-Li Sun, Tolgor Bau","doi":"10.47371/mycosci.2023.03.001","DOIUrl":"https://doi.org/10.47371/mycosci.2023.03.001","url":null,"abstract":"<p><p><i>Rhodocybe</i> subasyae is described from northeast China as a new species with both morphological and molecular characteristics, which is morphologically characterized by its tricholomatoid basidiomata, orange white to beige red pileus, adnexed to sinuate lamellae and long clavate branched cheilocystidia within section <i>Rufobrunnea</i>. The phylogenetic tree based on internal transcribed spacers of rDNA (nrITS) sequences using Bayesian methods showed that new taxon was distinguished from other species of <i>Rhodocybe</i>.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"64 3","pages":"96-100"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/1c/e7/64_mycosci.2023.03.001.PMC10308067.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9737067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycosciencePub Date : 2023-01-01DOI: 10.47371/mycosci.2022.12.003
Takashi Yaguchi
{"title":"Polymerase chain reaction-based methods for the detection of heat-resistant ascomycetous fungi.","authors":"Takashi Yaguchi","doi":"10.47371/mycosci.2022.12.003","DOIUrl":"https://doi.org/10.47371/mycosci.2022.12.003","url":null,"abstract":"<p><p>There is increasing incidence of food spoilage and health hazards caused by heat-resistant fungi belonging to the genera <i>Byssochlamys</i>, <i>Thermoascus</i>, and <i>Neosartorya</i>, among others. Their ascospores cannot be sterilized by heating the food. The microbiological risk assessment studies of these fungi during the production of food and beverages indicated that these fungal species or genera in food are associated with different health risks. Therefore, it is necessary to distinguish <i>Byssochlamys</i>, <i>Thermoascus</i>, and <i>Neosartorya</i> from other fungi in the food industry. These genera can be identified by sequence analysis of housekeeping genes such as β-tubulin, but the process is costly and time-consuming. Therefore, rapid and simple PCR-based methods have been developed using specific primer sets for genus- or species-level identification. PCR amplification products are observed to be specific for each of these genera or species and do not cross-react with other fungi associated with food spoilage and environmental contamination. These identification methods are simple, rapid, and highly specific, making them feasible for use in the quality management of food production plants.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"64 2","pages":"47-54"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/28/c0/MYC-64-047.PMC10165168.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9458142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycosciencePub Date : 2023-01-01DOI: 10.47371/mycosci.2023.04.001
Li-Qiong Cai, Chang-Lin Zhao
{"title":"Molecular phylogeny and morphology reveal a new wood-rotting fungal species, <i>Sistotrema yunnanense</i> sp. nov. from the Yunnan-Guizhou Plateau.","authors":"Li-Qiong Cai, Chang-Lin Zhao","doi":"10.47371/mycosci.2023.04.001","DOIUrl":"https://doi.org/10.47371/mycosci.2023.04.001","url":null,"abstract":"<p><p>Wood-rotting fungi are important components of woody plant ecosystems and play an active role in the decomposition and turnover of nutrients from wood, and are among the major groups of <i>Basidiomycota</i>. In this study, a new species of wood-rotting fungus, <i>Sistotrema yunnanense</i>, was proposed based on morphological characteristics and molecular evidence. It is characterized by resupinate basidiomata, a monomitic hyphal system having generative hyphae with clamp connections, suburniform to urniform basidia, and short-cylindrical to oblong ellipsoid basidiospores (4.5-6.5 × 3-4 µm). Phylogenetic analyses performed using the large subunit nuc rDNA indicated that <i>S. yunnanense</i> was nested within the genus <i>Sistotrema s.l.</i> of the family <i>Hydnaceae</i>, within the order <i>Cantharellales</i>.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"64 3","pages":"101-108"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/37/14/64_mycosci.2023.04.001.PMC10308066.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9743471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
MycosciencePub Date : 2023-01-01DOI: 10.47371/mycosci.2022.12.004
Yukako Hattori, Lynn Nakano, Chiharu Nakashima
{"title":"Taxonomic re-examination of Japanese isolates of the genus <i>Lasiodiplodia</i>.","authors":"Yukako Hattori, Lynn Nakano, Chiharu Nakashima","doi":"10.47371/mycosci.2022.12.004","DOIUrl":"https://doi.org/10.47371/mycosci.2022.12.004","url":null,"abstract":"<p><p><i>Lasiodiplodia</i> species cause dieback, shoot blight, fruit rot, and stem-end rot of woody plants. Recently, studies on taxonomic revision of <i>Lasiodiplodia</i> species have been conducted globally and have led to more clarification of the species diversity of this genus. However, only three species of the genus have been reported in Japan. To evaluate the species diversity of <i>Lasiodiplodia</i> in Japan, we re-examined the taxonomical positions of 30 isolates based on their morphological and cultural characteristics and phylogenetic relationships. Phylogenetic analyses were performed using a matrix comprising ITS, <i>tef1, tub2</i>, and <i>rpb2</i> sequences, and the results indicate that the examined Japanese isolates belong to six species: <i>Lasiodiplodia brasiliensis, L. hormozganensis, L. pseudotheobromae, L. thailandica, L. theobromae</i>, and <i>Lasiodiplodia</i> sp. Moreover, three of these species were newly added to the Japanese mycoflora.</p>","PeriodicalId":18780,"journal":{"name":"Mycoscience","volume":"64 2","pages":"74-82"},"PeriodicalIF":1.4,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/cd/9b/MYC-64-074.PMC10165170.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9454642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}