Monatshefte Fur Chemie最新文献_第3页

Enzyme prodrug therapy: cytotoxic potential of paracetamol turnover with recombinant horseradish peroxidase. 酶原药疗法：重组辣根过氧化物酶转化扑热息痛的细胞毒性潜力。

IF 1.7 4区化学

Monatshefte Fur Chemie Pub Date : 2021-01-01 Epub Date: 2021-10-05 DOI: 10.1007/s00706-021-02848-x

Diana Humer, Oliver Spadiut

{"title":"Enzyme prodrug therapy: cytotoxic potential of paracetamol turnover with recombinant horseradish peroxidase.","authors":"Diana Humer, Oliver Spadiut","doi":"10.1007/s00706-021-02848-x","DOIUrl":"10.1007/s00706-021-02848-x","url":null,"abstract":"Targeted cancer treatment is a promising, less invasive alternative to chemotherapy as it is precisely directed against tumor cells whilst leaving healthy tissue unaffected. The plant-derived enzyme horseradish peroxidase (HRP) can be used for enzyme prodrug cancer therapy with indole-3-acetic acid or the analgesic paracetamol (acetaminophen). Oxidation of paracetamol by HRP in the presence of hydrogen peroxide leads to N-acetyl-p-benzoquinone imine and polymer formation via a radical reaction mechanism. N-acetyl-p-benzoquinone imine binds to DNA and proteins, resulting in severe cytotoxicity. However, plant HRP is not suitable for this application since the foreign glycosylation pattern is recognized by the human immune system, causing rapid clearance from the body. Furthermore, plant-derived HRP is a mixture of isoenzymes with a heterogeneous composition. Here, we investigated the reaction of paracetamol with defined recombinant HRP variants produced in E. coli, as well as plant HRP, and found that they are equally effective in paracetamol oxidation at a concentration ≥ 400 µM. At low paracetamol concentrations, however, recombinant HRP seems to be more efficient in paracetamol oxidation. Yet upon treatment of HCT-116 colon carcinoma and FaDu squamous carcinoma cells with HRP-paracetamol no cytotoxic effect was observed, neither in the presence nor absence of hydrogen peroxide.Supplementary information: The online version contains supplementary material available at 10.1007/s00706-021-02848-x.","PeriodicalId":18766,"journal":{"name":"Monatshefte Fur Chemie","volume":"152 11","pages":"1389-1397"},"PeriodicalIF":1.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8542555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10255306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Voltammetric determination of heavy metals in honey bee venom using hanging mercury drop electrode and PLA/carbon conductive filament for 3D printer. 用垂汞电极和3D打印机用PLA/碳导电长丝伏安法测定蜂毒中的重金属。

IF 1.8 4区化学

Monatshefte Fur Chemie Pub Date : 2021-01-01 Epub Date: 2021-01-16 DOI: 10.1007/s00706-020-02725-z

Marta Choinska, Vojtěch Hrdlička, Ivana Šestáková, Tomáš Navrátil

{"title":"Voltammetric determination of heavy metals in honey bee venom using hanging mercury drop electrode and PLA/carbon conductive filament for 3D printer.","authors":"Marta Choinska, Vojtěch Hrdlička, Ivana Šestáková, Tomáš Navrátil","doi":"10.1007/s00706-020-02725-z","DOIUrl":"https://doi.org/10.1007/s00706-020-02725-z","url":null,"abstract":"A new method for determination of selected heavy metals (Cd, Pb, Cu, Zn, and Ni) in honey bee venom was developed. Heavy metals are metabolized and incorporated into bee products, including honey and honey bee venom (apitoxin). Their composition reflects contamination of \"bee environment\", providing information about heavy metal contamination in the neighborhood of human dwellings. Moreover, assessment of bee products contamination is relevant for medicine, as they are a tool for promising therapeutic and chemoprophylactic strategies against COVID-19 (SARS-CoV-2). Owing to the complicated matrix, the developed method consists of wet mineralization with sulfuric acid, nitric acid, under increased temperature, and pressure and subsequent repeated boiling with concentrated nitric acid. Determination of the selected metals was carried out by anodic or cathodic stripping voltammetry on two types of electrodes: pen-type hanging mercury drop electrode (HMDE) and PLA filament with carbon conductive admixture (PLA-C) for 3D printer. Contents of lead and cadmium in all analyzed bee venom samples were on the level of mg kg-1, of nickel and copper about ten times higher, and of zinc on the level of g kg-1. The results achieved using HMDE were recorded with average relative standard deviation (RSD) 5.4% (from 3.2% to 8.6%) and using PLA-C 11.8% (from 6.5% to 18.0%). The results achieved using both electrodes proved to be equivalent with statistical probability higher than 95%.Graphic abstract: ","PeriodicalId":18766,"journal":{"name":"Monatshefte Fur Chemie","volume":"152 1","pages":"35-41"},"PeriodicalIF":1.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s00706-020-02725-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38853016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

A trigonal coordination of Au(I) phosphane complexes stabilized by O-H ^⋯ X (X = Cl^-, Br^-, I^-) interactions. 通过 O-H ⋯ X（X = Cl-、Br-、I-）相互作用稳定的 Au（I）膦烷配合物的三方配位。

IF 1.7 4区化学

Monatshefte Fur Chemie Pub Date : 2021-01-01 Epub Date: 2021-09-15 DOI: 10.1007/s00706-021-02843-2

Petra Gründlinger, Cezarina Cela Mardare, Thorsten Wagner, Uwe Monkowius

引用次数: 0

Tribromoisocyanuric acid as a useful oxidant for the synthesis of 1,3-diynes via Glaser coupling 三溴异氰尿酸在激光偶联法合成1,3-二炔中的氧化剂作用

IF 1.8 4区化学

Monatshefte Fur Chemie Pub Date : 2020-09-10 DOI: 10.1007/S00706-020-02673-8

Vitor S. C. de Andrade, M. C. D. Mattos

引用次数: 1

Chemophobia versus the identity of chemists: heroes of chemistry as an effective communication strategy. 化学恐惧症与化学家的身份认同：化学英雄作为一种有效的传播策略。

IF 1.7 4区化学

Monatshefte Fur Chemie Pub Date : 2020-01-01 Epub Date: 2020-06-08 DOI: 10.1007/s00706-020-02633-2

Radek Chalupa, Karel Nesměrák

引用次数: 0

Assembling the prenylneoflavone system through a Pechmann condensation/Mitsunobu reaction/Claisen rearrangement/olefin cross-metathesis sequence. 通过Pechmann缩合/Mitsunobu反应/Claisen重排/烯烃交叉复分解序列组装戊烯基新黄酮体系。

IF 1.8 4区化学

Monatshefte Fur Chemie Pub Date : 2020-01-01 Epub Date: 2020-04-28 DOI: 10.1007/s00706-020-02584-8

Oleg A Lozinski, J Bistodeau, C Bennetau-Pelissero, Vladimir P Khilya, S Shinkaruk

引用次数: 2

Synthesis and characterization of TADDOL-based chiral group six PNP pincer tricarbonyl complexes. TADDOL手性第六族PNP钳形三羰基配合物的合成与表征。

IF 1.7 4区化学

Monatshefte Fur Chemie Pub Date : 2019-01-01 Epub Date: 2018-10-20 DOI: 10.1007/s00706-018-2281-0

Sara R M M de Aguiar, Christian Schröder-Holzhacker, Jan Pecak, Berthold Stöger, Karl Kirchner

引用次数: 0

Methyl glycosides via Fischer glycosylation: translation from batch microwave to continuous flow processing. 通过Fischer糖基化的甲基糖苷：从分批微波转化为连续流处理。

IF 1.7 4区化学

Monatshefte Fur Chemie Pub Date : 2019-01-01 Epub Date: 2018-11-17 DOI: 10.1007/s00706-018-2306-8

Jonas Aronow, Christian Stanetty, Ian R Baxendale, Marko D Mihovilovic

引用次数: 0

Persistent radical anions in the series of peri-arylenes: broadband light absorption until far in the NIR and purely organic magnetism. 环芳烃系列中的持久性自由基阴离子:直到近红外波段的宽带光吸收和纯有机磁性。

IF 1.8 4区化学

Monatshefte Fur Chemie Pub Date : 2019-01-01 Epub Date: 2019-05-02 DOI: 10.1007/s00706-019-02404-8

Heinz Langhals, Ulrike Ritter-Faizade, Philipp Stadler, Marek Havlicek, Alexander Hofer, Niyazi Serdar Sariciftci

引用次数: 4

Synthesis and characterization of bis- and tris-carbonyl Mn(I) and Re(I) PNP pincer complexes. 双和三羰基Mn（I）和Re（I）PNP钳形配合物的合成与表征。

IF 1.7 4区化学

Monatshefte Fur Chemie Pub Date : 2019-01-01 Epub Date: 2018-10-20 DOI: 10.1007/s00706-018-2307-7

Mathias Glatz, Jan Pecak, Lena Haager, Berthold Stoeger, Karl Kirchner

{"title":"Synthesis and characterization of bis- and tris-carbonyl Mn(I) and Re(I) PNP pincer complexes.","authors":"Mathias Glatz, Jan Pecak, Lena Haager, Berthold Stoeger, Karl Kirchner","doi":"10.1007/s00706-018-2307-7","DOIUrl":"10.1007/s00706-018-2307-7","url":null,"abstract":"Abstract: A series of neutral bis- and cationic tris-carbonyl complexes of the types cis-[M(κ3 P,N,P-PNP)(CO)2Y] and [M(κ3 P,N,P-PNP)(CO)3]+ was prepared by reacting [M(CO)5Y] (M = Mn, Re; Y = Cl or Br) with PNP pincer ligands derived from the 2,6-diaminopyridine, 2,6-dihydroxypyridine, and 2,6-lutidine scaffolds. With the most bulky ligand PNPNH-tBu, the cationic square-pyramidal 16e bis-carbonyl complex [Mn(PNPNH-tBu)(CO)2]+ was obtained. In contrast, in the case of rhenium, the 18e complex [Re(PNPNH-tBu)(CO)3]+ was formed. The dissociation of CO was studied by means of DFT calculation revealing in agreement with experimental findings that CO release from [M(κ3 P,N,P-PNP)(CO)3]+ is in general endergonic, while for [Mn(κ3 P,N,P-PNPNH-tBu)(CO)3]+, this process is thermodynamically favored. X-ray structures of representative complexes are provided.Graphical abstract: ","PeriodicalId":18766,"journal":{"name":"Monatshefte Fur Chemie","volume":"150 1","pages":"111-119"},"PeriodicalIF":1.7,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6320747/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36879192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0