Molecular Biology Reports最新文献

{"title":"Methylation status of GSTP1 and APC promoter genes as potential biomarkers for early detection and tumor aggressiveness in prostate cancer: insights from a Moroccan cohort.","authors":"Imane Mharrach, Mouna Aqerrout, Kaoutar Anouar Tadlaoui, Abdelilah Laraqui, Anouar El Ghazzaly, Moulay Mustapha Ennaji","doi":"10.1007/s11033-025-10812-9","DOIUrl":"https://doi.org/10.1007/s11033-025-10812-9","url":null,"abstract":"<p><strong>Background: </strong>Prostate cancer (PCa) is one of the leading causes of morbidity and mortality among men worldwide. While age, race and family history are established risk factors, their molecular underpinning remain poorly understood. Aberrant promoter methylation is a well-known mechanism for silencing tumor suppressor genes in PCa. This study evaluates the methylation status of glutathione-S-transferase-P1 (GSTP1) and adenomatous polyposis coli (APC) genes in Moroccan men with PCa, assessing their potential as biomarkers for early detection and tumor characterization.</p><p><strong>Methods: </strong>A total of 70 prostate cancer tissue samples and 50 control tissues were analyzed. Methylation specific PCR (MSP) was used to evaluate the promoter methylation status of GSTP1 and APC, The results were correlated with age, PSA levels, Gleason score, tumor stage, and life style factors.</p><p><strong>Results: </strong>GSTP1 promoter methylation was observed in 79% of PCa cases and was significantly associated with lower Gleason scores (= 6: 64%, P = 0.006) and early T-stages tumors (T1 and T2, P = 0.002). APC methylation was found in 63% of cases and showed a significant association with higher Gleason scores (> 6: 59%, P = 0.007). Although APC methylation did not significantly correlate with pathological T-stage, it was more frequent in early stages (T1: 45%, T2: 34%). No significant associations were observed between GSTP1 or APC methylation and age, PSA levels, smoking, or alcohol consumption.</p><p><strong>Conclusion: </strong>promoter methylation of GSTP1 and APC is frequent in Moroccan prostate cancer patients. GSTP1 methylation appears to be associated with early stage disease, Whereas APC methylation is linked to tumor aggressiveness. These findings suggest the potential utility of GSTP1 and APC promoter methylation as biomarkers for prostate cancer detection and characterization, further validation with larger cohorts and gene expression analysis is needed to confirm their clinical relevance.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"711"},"PeriodicalIF":2.6,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interaction between COX-2 and endoplasmic reticulum stress is involved in liver ischemia-reperfusion injury in mice.","authors":"Chen Liang, Liping Guo, Xinyan Tian, Zhidan Mu, Mingyang Chen, Yarong Hu, Juan Su","doi":"10.1007/s11033-025-10814-7","DOIUrl":"https://doi.org/10.1007/s11033-025-10814-7","url":null,"abstract":"<p><strong>Objective: </strong>By establishing a mouse model of hepatic ischemia-reperfusion injury (HIRI), to investigate the effects of cyclooxygenase-2 (COX-2) and endoplasmic reticulum stress (ERS) on HIRI and their interaction in HIRI.</p><p><strong>Methods: </strong>C57BL/6J male mice were divided into four groups: Sham operation group (Sham group), hepatic ischemia-reperfusion (HIR) group, hepatic ischemia-reperfusion + Salubrinal (ER stress inhibitor) pretreatment group (HIR + Sal group), hepatic ischemia-reperfusion + NS398 (inhibitor of COX-2) pretreatment group (HIR + NS398 group). The levels of Aspartate transaminase (AST) and Alanine transaminase (ALT) in serum, the activity of Super Oxide Dismutase (SOD) and the content of malondialdehyde (MDA) in liver tissue were detected. The mRNA and protein expression of Glucose-regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), phosphorylated eukaryotic translation initiation factor 2α (p-eIF2α) and COX-2/PTGS (Prostaglandin-endoperoxide synthase) in liver tissue were detected. The morphological changes and apoptosis of liver tissue were observed.</p><p><strong>Results: </strong>Compared with the Sham group, the serum ALT and AST levels of the HIR group were increased, the SOD activity in the liver tissue was decreased, the MDA content and the expression of COX-2, p-eIF2α, GRP78 and CHOP protein were increased, and the staining results showed that hepatic sinusoidal congestion, a large number of hepatocyte death, inflammatory cell infiltration and apoptotic cells were significantly increased. The levels of ALT and AST decreased after Sal or NS398 pretreatment. SOD activity was increased, MDA content, COX-2, GRP78, CHOP mRNA and protein were decreased in liver tissue, and staining showed that liver tissue injury was improved.</p><p><strong>Conclusion: </strong>In mice with HIR, COX-2 interacts with ERS to cause liver injury by regulating oxidative stress and apoptosis.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"710"},"PeriodicalIF":2.6,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential gene expression in the intestines of Chinese mitten crabs with three carapace colors.","authors":"Qi Zhu, Maolei Wei, Xinxin Chen, Zhichao Rong, Dongran Yang, Xiaowu Chen, Xugan Wu","doi":"10.1007/s11033-025-10808-5","DOIUrl":"https://doi.org/10.1007/s11033-025-10808-5","url":null,"abstract":"<p><strong>Background: </strong>Carapace color in Chinese mitten crab (Eriocheir sinensis) influences both commercial value and physiological traits, yet its molecular basis remains unclear. The intestine plays a key role in pigment absorption and metabolism, but its involvement in pigmentation has been understudied. This study aimed to investigate intestinal gene expression differences among red, white, and green carapace strains.</p><p><strong>Methods and results: </strong>We sequenced intestinal RNA from red, white, and green morphs (n = 3 per group) on an Illumina NovaSeq platform. Following fastp quality control and Salmon quantification, 6,214 genes were identified as differentially expressed (|log2FC| ≥ 1, FDR < 0.05). Functional enrichment linked these genes to pigment transport and biosynthesis pathways, including ABC transporters, SLC carriers, carotenoid binding, and tyrosine metabolism. Seven representative DEGs (CRCN‑A1, CRCN-A2, ABCC4, ABCF1, slc23a1, slc49a4, TYROB) were validated by qRT‑PCR, with expression trends congruent to RNA‑seq results.</p><p><strong>Conclusion: </strong>Intestinal gene expression contributes to carapace pigmentation through distinct carotenoid‑transport and melanin pathways. These insights offer molecular targets for selective breeding and functional validation.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"712"},"PeriodicalIF":2.6,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"5-mC DNA methylation in neurodevelopment: from molecular mechanisms to therapeutic implications.","authors":"Dishu Huang, Wenjie Zhao, Hong Sun, Chen Yang, Li Jiang","doi":"10.1007/s11033-025-10809-4","DOIUrl":"https://doi.org/10.1007/s11033-025-10809-4","url":null,"abstract":"<p><p>5-mC DNA methylation is a fundamental epigenetic modification that plays a crucial role in neurodevelopment and neurological disorders. This review synthesizes the current understanding of 5-mC DNA methylation in neural system development and its implications in neurodevelopmental disorders. During normal neural development, 5-mC methylation precisely regulates neural stem cell differentiation and neuronal maturation through DNA methyltransferases (DNMTs) and methyl-CpG-binding domain (MBD) proteins. Disruption of these methylation patterns contributes to various neurodevelopmental disorders. In autism spectrum disorder (ASD), altered methylation patterns in specific genes like SHANK family and genome-wide methylation changes have been identified as potential diagnostic biomarkers. In fragile X syndrome, CGG trinucleotide repeat expansion increases methylation of the FMR1 gene promoter, leading to FMRP protein deficiency. Rett syndrome, primarily caused by MECP2 mutations, involves disrupted methylation-dependent transcriptional regulation. In epilepsy, DNA methylation abnormalities affect multiple epilepsy-related genes and may influence treatment responses to ketogenic diets. Despite these advances, the field faces significant challenges including tissue specificity issues, technical limitations in methylation detection, and therapeutic targeting difficulties. This review also discusses future perspectives, emphasizing the potential of DNA methylation as a therapeutic target and biomarker for neurodevelopmental disorders. Understanding these methylation mechanisms could lead to novel diagnostic tools and therapeutic strategies for various neurological conditions.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"713"},"PeriodicalIF":2.6,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New mini-satellite tandem repeat markers for strain typing of Mycobacterium avium subsp. paratuberculosis.","authors":"Elika Faraj Tabrizi, Mohammad Reza Zolfaghari, Nader Mosavari, Razieh Nazari, Keyvan Tadayon","doi":"10.1007/s11033-025-10792-w","DOIUrl":"https://doi.org/10.1007/s11033-025-10792-w","url":null,"abstract":"<p><strong>Background: </strong>The multiplicity of infection sources along with the genetically homogenous nature of Mycobacterium avium subsp. paratuberculosis (MAP) necessitates the application of high-discriminative fingerprinting techniques to establish epidemiological links and control of paratuberculosis in ruminants. The present study aimed to develop a simple Multiple-Locus Variable-Number Tandem-Repeat Analysis (MLVA) assay suitable for low-budget laboratory settings.</p><p><strong>Methods: </strong>A bioinformatics screening was conducted on three annotated MAP genomes (K10, MAP4, and MAP-JIII-386) leading to the detection of 7 potential polymorphic variable-number tandem repeats (VNTRs). A MAP collection of the three aforementioned strains, 30 field isolates and 2 laboratory strains from Iran plus 59 global Mycobacterium avium complex strains were subsequently genotyped at laboratory or in situ. Findings were validated against the French INRAE MLVA typing system (Thibault's scheme) dedicated to typing Mycobacterium avium subsp. paratuberculosis, hominissuis, avium, and silvaticum.</p><p><strong>Results: </strong>Fourty-one MLVA types were detected by our suggested MLVA scheme, compared to 45 types found by Thibault's scheme. The calculated discriminatory power of the MLVA for the former and the latter was 0.93 and 0.95, respectively.</p><p><strong>Conclusion: </strong>The suggested MLVA scheme discriminated between isolates with identical types determined by Thibault's scheme. Noting the fact that strains grouped in INMV types 2, 3, 4, 5, 33, 39, 68, 82, 131, 219, 220, New1 and New 25 were further resolved into sub-groups, we suggest considering VNTR 1067 (DI = 0.71), MAP 4356 (DI = 0.68), MATR 7 (DI = 0.53), MAP 3824 (DI = 0.56) and MATR 6 (DI = 0.49), in any future upgrading of the current version of the Thibault's scheme.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"709"},"PeriodicalIF":2.6,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silencing lncRNA MCM3AP-AS1 protects against cisplatin-induced cell death in A549 lung cancer cells.","authors":"Ibrahim Bozgeyik, Demet Tasdemir, Esra Bozgeyik, Haydar Bagis, Serdar Oztuzcu, Ahmet Ferudun Isik","doi":"10.1007/s11033-025-10822-7","DOIUrl":"https://doi.org/10.1007/s11033-025-10822-7","url":null,"abstract":"<p><strong>Background: </strong>Lung cancer remains the leading cause of cancer-related mortality worldwide, largely due to the development of resistance to standard chemotherapeutic agents such as cisplatin. Recent evidence has highlighted the regulatory role of long non-coding RNAs (lncRNAs) in mediating drug resistance mechanisms. In this study, we aimed to reveal the role of MCM3AP-AS1 in cisplatin-induced cell death in lung cancer cells.</p><p><strong>Methods and results: </strong>As a result, we found that MCM3AP-AS1, a lncRNA previously associated with poor prognosis in various cancers, was significantly upregulated in A549 lung cancer cells following cisplatin treatment. Using RNA interference-mediated silencing of MCM3AP-AS1, we observed a marked increase in cell viability and a decrease in apoptosis, as determined by MTT assay, annexin V/PI flow cytometry, and acridine orange/ethidium bromide staining.</p><p><strong>Conclusions: </strong>These findings suggest that MCM3AP-AS1 may be a crucial modulator of cisplatin-induced apoptosis in lung cancer cells.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"708"},"PeriodicalIF":2.6,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Priming effect of photobiomodulation of mesenchymal stem cells on extracellular vesicles for regenerative medicine.","authors":"Maria Emília Mota, Márcia Martins Marques, Fábio Abreu Alves, Rebeca Boltes Cecatto, Suely Kunimi Kubo Ariga, Maria Stella Moreira","doi":"10.1007/s11033-025-10791-x","DOIUrl":"https://doi.org/10.1007/s11033-025-10791-x","url":null,"abstract":"<p><p>Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) (MSCs-derived EVs) have shown promising outcomes in repairing damaged tissues across various disease models. Combining MSCs-derived EVs with advanced technologies could further enhance their use as a viable cell-free therapy in medical applications. Photobiomodulation (PBM) has shown a synergistic effect with EVs in improving tissue regeneration. This pioneer review aimed to evaluate the entire scope available in the current literature on the PBM priming effect on MSCs-derived EVs. Two independent reviewers conducted a literature search in online databases PubMed/MEDLINE, Web of Science, Scopus, EMBASE and LIVIVO. The inclusion criteria were: (1) studies investigating PBM and MSCs-derived EVs and (2) all language and data. Of 213 found articles, 8 (eight) articles were selected for a complete reading. After the complete reading, 6 (six) articles were selected for data extraction. In general, the studies reported no deleterious effects of PBM on EVs. Furthermore, in vitro, PBM increased concentration of EVs, angiogenic factors, anti-apoptotic protein levels and cell survival. In vivo, PBM and EVs demonstrated positive effects in skin wound healing and pulp regeneration. These studies highlighted the promising potential of combining both therapies (PBM and MSCs-derived EVs) as an innovative strategy in regenerative medicine. However, significant challenges remain, including the need for standardization of EVs isolation and characterization methods, as well as the evaluation of laser irradiation parameters across diverse experimental conditions. Additionally, a deeper understanding of the underlying mechanisms of PBM effects on EVs is crucial to optimize this approach and fully harness its therapeutic potential.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"707"},"PeriodicalIF":2.6,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morpho-biochemical and molecular characterization of Bacillus subtilis and Priestia megaterium isolates from eastern Indian farmlands.","authors":"Sujaan Aehsas, Jyoti Prakash Sahoo, Neelanjana Choudhury, Shaikh Nausad Hossain, Anjali Lata Sahoo, Sunil Kumar Sunani","doi":"10.1007/s11033-025-10796-6","DOIUrl":"https://doi.org/10.1007/s11033-025-10796-6","url":null,"abstract":"<p><strong>Background: </strong>The study aimed to investigate the microbial diversity of soils from distinct environments, an urban park (SJ1) and an agricultural research field (SJ2) using serial dilution and spread plate techniques on nutrient agar, to identify region-specific bacterial strains with potential agricultural or biotechnological applications.</p><p><strong>Methods and results: </strong>Bacterial isolates were purified through selective sub-culturing, characterized via Gram staining and biochemical tests (MR-VP, catalase, oxidase), and preserved at 4 °C. Genomic DNA was extracted, PCR-amplified targeting the 16S rRNA gene, and sequenced for BLAST-based identification. Phylogenetic analysis (MEGA 12, neighbor-joining, 1000 bootstraps) and evolutionary rate estimation (maximum likelihood, gamma-distributed site variation) were performed to assess genetic relationships and divergence. Morphological and biochemical analyses revealed Gram-positive rods in both isolates, exhibiting catalase positive, oxidase negative, methyl red negetive, and Voges-Proskauer positive activity. Genomic DNA was extracted, and the 16S rRNA gene was amplified (~ 1500 bp), sequenced, and analyzed via BLASTN. SJ1 showed 99.84-99.92% identity with Bacillus spp., while SJ2 matched Priestia megaterium (99.92%). Phylogenetic analysis placed SJ1 within Bacillus subtilis strain SBIS1 (GenBank: PV523294.1), and SJ2 within P. megaterium strain PIIS1 (GenBank: PV523528.1), indicating distinct evolutionary lineages. Maximum likelihood estimation revealed differing evolutionary rates, i.e., SJ1 exhibited high rate heterogeneity, while SJ2 showed uniform rates. Mean relative evolutionary rates across five discrete gamma categories for SJ1 ranged from 0.08 to 2.75, while for SJ2 they ranged from 0.90 to 1.10. SJ1 showed 25.23% (A), 20.09% (T), 23.43% (C), and 31.26% (G); while SJ2 had 24.87% (A), 21.26% (T), 24.20% (C), and 29.68% (G). Maximum log-likelihood values were -3315.740 for SJ1 (1268 aligned sites) and -1034.491 for SJ2 (750 aligned sites), confirming robust phylogenetic inference.</p><p><strong>Conclusion: </strong>The study successfully identified distinct bacterial strains with unique evolutionary rates, genetic compositions, and robust divergence between the isolates, supporting their suitability for further agricultural or biotechnological exploration.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"706"},"PeriodicalIF":2.6,"publicationDate":"2025-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144619087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variable roles of miRNA- and apoptosis-linked genes in invasive breast cancer: expression patterns, clinicopathological associations, and prognostic significance.","authors":"Luděk Záveský, Eva Jandáková, Vít Weinberger, Luboš Minář, Radovan Turyna, Ondřej Slanař","doi":"10.1007/s11033-025-10739-1","DOIUrl":"https://doi.org/10.1007/s11033-025-10739-1","url":null,"abstract":"<p><strong>Introduction: </strong>Breast cancer is the most common cancer and the leading cause of cancer-related death in women. Differential gene expression can help identify genes involved in carcinogenesis or serve as biomarkers.</p><p><strong>Methods: </strong>This study provides a comprehensive evaluation of the gene expression focusing on apoptosis-related genes, in invasive breast carcinoma of no specific type compared with benign tissue. The gene expression of nine candidate genes identified as potential targets of certain microRNAs suggested as biomarkers and known for their role in apoptosis, and two additional apoptosis-related genes identified in the screening was evaluated using qPCR together with external datasets.</p><p><strong>Results: </strong>Screening of 92 apoptosis-related genes identified several dysregulated genes including downregulated BCL2L2 and upregulated BIRC5 genes, which were further confirmed as tumor suppressor and as an oncogene, respectively. Among the miRNA-related genes, HMGA2 and RAB22A were overexpressed, while ATF2, PPM1L, VPS4A, ZEB1, and ZFP36L1 were underexpressed. The BIRC5/BCL2L2 gene signature provided AUC of 0.975, sensitivity of 93.10% and specificity of 96.43%. Increased BIRC5 expression was associated with higher tumor grades and Ki-67-positive samples while decreased levels of BCL2L2 were associated with Ki-67-positive samples. Luminal A and B samples were distinguished by the differential expression of these two genes. The high expression of HMGA2 and BIRC5 genes was observed as a negative prognostic factor for both overall survival (OS) and progression-free survival (PFS) with a favorable OS difference of ~ 1 year for HMGA2 and 1.2 years for BIRC5 in the case of their low expression. External validation identified ZEB1 as a positive and BIRC5 as a negative prognostic factor for both overall and disease-free survival.</p><p><strong>Conclusion: </strong>The results highlighted genes with possible roles in apoptosis and acting in breast carcinogenesis. In particular, BIRC5 was shown as important oncogene and ZEB1 as a tumor suppressor in invasive breast cancer. Further studies are warranted to evaluate the potential of the investigated genes as biomarkers or therapeutic targets, with possible implications for breast cancer diagnosis and treatment.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"703"},"PeriodicalIF":2.6,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastric microbiome-derived Lacticaseibacillus casei strain RIGLD MG-1 relieves Helicobacter pylori-induced inflammation in gastric epithelial cells in vitro.","authors":"Masoomeh Ghasemipoor, Mohammad Yaghoubi-Avini, Masoumeh Azimirad, Ali Nabavi-Rad, Mehdi Azizmohammad Looha, Michael Doulberis, Christian Schulz, Abbas Yadegar","doi":"10.1007/s11033-025-10817-4","DOIUrl":"https://doi.org/10.1007/s11033-025-10817-4","url":null,"abstract":"<p><strong>Background: </strong>The burden of Helicobacter pylori infection is exacerbated by rising antibiotic resistance. Probiotics, especially Lactobacillus species, have shown potential as adjunctive therapies for H. pylori infection. This study aimed to isolate a Lactobacillus strain from the gastric microbiome of healthy individuals and assess its probiotic properties against H. pylori.</p><p><strong>Methods and results: </strong>Gastric biopsies were obtained from a cohort of 10 subjects, and used for bacterial isolation. The Lactobacillus strain was identified to species level using PCR and sequencing, followed by safety assessments. MTT assay was employed to measure AGS cell viability after exposure to various concentrations of H. pylori, as well as live and pasteurized Lactobacillus. Anti-inflammatory properties of the probiotic strain were assessed in AGS cells using RT-qPCR and ELISA. Additionally, the strain's potential to inhibit H. pylori adhesion and invasion was examined. Probiotic Lacticaseibacillus casei strain RIGLD MG-1 was isolated and characterized as non-pathogenic and found to be tolerant to acidic and bile-rich environments, and susceptible to several antibiotics. Live and pasteurized L. casei downregulated the expression of NF-κB, IL-8, TNF-α, and β-catenin, meanwhile upregulated the expression of IL-10 in H. pylori-treated cells. They also alleviated H. pylori-induced proinflammatory response by lowering IL-8 and TNF-α, and boosting IL-10 production. Additionally, both probiotic forms inhibited H. pylori adhesion and invasion in AGS cells.</p><p><strong>Conclusion: </strong>Our results show that L. casei strain RIGLD MG-1 is safe and demonstrates significant immunomodulatory and anti-adhesion effects, making it a potential probiotic for use as adjunctive therapy to mitigate H. pylori-induced inflammation.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"702"},"PeriodicalIF":2.6,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144608837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}