Molecular Biology Reports最新文献

{"title":"Regulation of the sRNA ncBCG427 on mycobacterial stress adaptation.","authors":"Kailun Zhang, Zejin Du, Zijian Wang, Yingyu Chen, Aizhen Guo","doi":"10.1007/s11033-025-10354-0","DOIUrl":"https://doi.org/10.1007/s11033-025-10354-0","url":null,"abstract":"<p><strong>Background: </strong>Mycobacterium tuberculosis complex (MTBC) comprises the primary pathogens responsible for tuberculosis in humans and animals, including the virulent Mycobacterium tuberculosis (M. tb) and Mycobacterium bovis (M. bovis). As a group of intracellular bacteria, MTBC has developed intricate mechanisms for infection and survival within host cells. Among these mechanisms, small non-coding RNAs (sRNAs) play essential roles in regulating post-transcriptional pathways that may aid in stress adaptation in mycobacteria. In our previous research, we identified a novel sRNA, ncBCG427, in BCG, the attenuated strain of M. bovis. This sRNA is differentially expressed under various stress conditions, and homologs exist in both M. tb and M. bovis.</p><p><strong>Method and results: </strong>To explore the regulatory capabilities of ncBCG427 on bacterial stress adaptation in the original BCG strain, we constructed overexpression (BCG_ncBCG427) and control (BCG_Vector) strains. Our findings demonstrated that ncBCG427 significantly enhanced BCG survival under carbon starvation and acid stress conditions, while it resulted in decreased survival under iron starvation conditions. Transcriptomic analyses revealed that overexpression of ncBCG427 substantially altered gene expression profiles in these three stress environments, with differentially expressed genes (DEGs) in the same pathways enriched for different stressors. Notably, we identified 22 DEGs that responded to two or three stress conditions, implicating their involvement in pathways related to the sulfur relay system, ribosome function, folate biosynthesis, and the biosynthesis of cofactors. This suggested that these genes may served as key regulators in ncBCG427-mediated stress adaptation. Furthermore, quantitative PCR (qPCR) was employed to verify the expression levels of 16 DEGs in BCG, with 14 of them matching homologous genes in M. tb. We then created a ncBCG427 overexpression strain in M. tb (MTB_ncBCG427) alongside a control strain (MTB_Vector). Interestingly, 4 of the 14 genes displayed consistent expression trends in both M. tb and BCG under different stress conditions.</p><p><strong>Conclusion: </strong>In conclusion, our study underscored the significant role of the sRNA ncBCG427 in enhancing mycobacterial survival under stress conditions and proposed potential target genes for further exploration into the mechanisms of stress adaptation in mycobacteria.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"317"},"PeriodicalIF":2.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic-induced gut dysbiosis: unraveling the gut-heart axis and its impact on cardiovascular health.","authors":"Navpreet Kaur, Pankaj Kumar, Mahadev Dhami, Khadga Raj Aran","doi":"10.1007/s11033-025-10425-2","DOIUrl":"https://doi.org/10.1007/s11033-025-10425-2","url":null,"abstract":"<p><p>Cardiovascular diseases (CVDs) remain the major cause of morbidity and mortality amongst people of all ages across the world. Research suggests that the initiation and progression of CVDs are associated with antibiotic-induced gut dysbiosis. Antibiotics are primarily intended to be used to treat bacterial infections, which can alter gut microbiota (GM) composition, by lowering the abundance of beneficial bacteria, like Firmicutes, Bacteroidetes, and increasing the profusion of Enterobacteriaceae, leading to harm on gut health. Additionally, it reduces short-chain fatty acids (SCFAs) and bile acid metabolism, increases trimethylamine N-oxide (TMAO) production, intestinal permeability allowing lipopolysaccharide (LPS) and TMAO into systemic circulation. SCFAs play a key role in lipid metabolism, inflammation, and strengthening of the intestinal barrier, and participate in CVDs through FFAR2 and FFAR3 receptors, whereas dysbiosis reduces SCFAs levels and worsens these effects. TMAO enhances oxidative stress, inflammation, endothelial dysfunction, and cholesterol dysregulation, thus worsening CVDs. Furthermore, LPS develops systemic inflammation, insulin resistance, and endothelial dysfunction by activating the NF-κB pathway. Dysbiosis also affects bile acid synthesis, disrupting lipid and glucose metabolism, further participating in the progression of CVDs. This article aims to explore the role of gut dysbiosis in various CVDs, including congenital heart disease, hypertension, valvular heart disease, coronary heart disease, and heart failure. Furthermore, this article aims to bridge the knowledge gap regarding the gut-heart axis by exploring how antibiotics alter the gut microbiota homeostasis, further contributing to the development of CVDs and therapeutic interventions that reduce cardiovascular risks and restore the gut microbiota homeostasis.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"319"},"PeriodicalIF":2.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-124a/Sirtuin 1 pathway inhibits autophagy to promote hepatocyte apoptosis in acute-on-chronic liver failure.","authors":"Hongrui Xu, Xin Wang, Yadong Wang, Chuan Shen, Luyuan Ma, Caiyan Zhao","doi":"10.1007/s11033-025-10373-x","DOIUrl":"10.1007/s11033-025-10373-x","url":null,"abstract":"<p><strong>Background: </strong>The roles of microRNAs in the regulation of autophagy and apoptosis in hepatic cells suggest that they may serve as novel biomarkers or therapeutic targets for various liver injuries. In this study, we aim to analyze whether miR-124a regulates autophagy and apoptosis in hepatic cells, particularly in acute-on-chronic liver failure (ACLF).</p><p><strong>Materials and methods: </strong>The plasma and liver tissues from the healthy control (HC) and ACLF patients were included. Moreover, LO2 cells were used to perform in vitro experiments. To measure hepatocyte apoptosis, a TUNEL kit was used. LPS, over-expression or knockdown, 3-methyladenine (3-MA) were used in vitro experiments. The expression levels of the autophagy related proteins (Beclin-1 and LC3), anti-apoptotic proteins (BAX and Bcl-2), Sirtuin 1 (SIRT1), and miR-124a were assessed using western blotting, ELISA, and qRT-PCR.</p><p><strong>Results: </strong>ACLF patients had significantly decreased expressions of SIRT1, Bcl-2, LC3, and Beclin-1 and significant upregulation of miR-124a and BAX in both plasma and liver tissues in comparison with the HC group. miR-124a was inversely correlated with autophagy markers and SIRT1, but positively correlated with apoptosis. Upon exposure to LPS, the levels of BAX and miR-124a were notably elevated, while Beclin-1, LC3, SIRT1, and Bcl-2 were notably downregulated in LO2 cells. These changes were further exaggerated in the presence of the miR-124a mimic and EX-527 compared to the miR-124a inhibitor and SRT1720 groups. Co-transfection of miR-124a inhibitor was able to partly counteract the pro-apoptotic effects of the autophagy inhibitor 3-MA.</p><p><strong>Conclusion: </strong>miR-124a downregulates SIRT1, thereby suppressing hepatocyte autophagy and consequently inducing apoptosis.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"314"},"PeriodicalIF":2.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11910420/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of Fusarium acuminatum causing root rot on sesame in Noutheast of China.","authors":"Qiyuan An, Hongsen Cheng, Huijie Sun, Yanbin Na, Dexue Gao","doi":"10.1007/s11033-025-10428-z","DOIUrl":"https://doi.org/10.1007/s11033-025-10428-z","url":null,"abstract":"<p><strong>Background: </strong>Sesame is an important oil-producing crop, possessing approximately 50% oil and 20% protein content, along with a variety of antioxidant substances, endowing it with high edible and medicinal value. However, fungal diseases, especially Fusarium wilt with a high incidence, pose a significant threat and severely limit the development of the sesame industry.</p><p><strong>Methods and results: </strong>In this study, we identified symptoms of sesame fusarium wilt as with the midrib as the boundary, half of the leaf turns yellow while the other half remains normal, plantwilted and rotting roots, and eventually plant wilting and death. To identify the pathogen responsible, we employed a multi-faceted approach. Morphologically, we examined the color of the colonies and the specific morphology of the conidia. Moreover, we constructed a phylogenetic tree based on the DNA sequences of ITS and TEF1-α. Through these combined efforts, the pathogen was identified as F. acutuminatum. Subsequently, pathogenicity tests were carried out, and the results unequivocally demonstrated that F. acutuminatum was capable of inducing the characteristic symptoms of Fusarium wilt in sesame seedlings, including root rot and leaf yellowing.</p><p><strong>Conclusions: </strong>This study identified key aspects of sesame Fusarium wilt. The determined symptoms and pathogen, along with proven pathogenicity, offer a theoretical basis for devising strategies to prevent and treat this disease, potentially enhancing sesame industry sustainability.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"315"},"PeriodicalIF":2.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143634300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Versatile functions of methyl-CpG-binding domain 2 (MBD2) in cellular characteristics and differentiation.","authors":"Rabia Tuana Lekesiz, Kasım Kağan Koca, Gizem Kugu, Zihni Onur Çalışkaner","doi":"10.1007/s11033-025-10411-8","DOIUrl":"https://doi.org/10.1007/s11033-025-10411-8","url":null,"abstract":"<p><p>Cellular differentiation is a vital process that results in cell specialization and functionalization, synchronized with the development and growth in multicellular organisms. Any fault in this process can bring about the emergence of various diseases. Gene expression controls cellular differentiation, but various epigenetic mechanisms play a pivotal role as well. For instance, DNA methylation and combined histone modifications, such as histone acetylation/deacetylation, are crucial in cellular differentiation. Methyl-CpG-Binding Domain 2 (MBD2), a highly conserved member of the MBD protein family, is considered a reader of DNA methylation and drives the crosstalk between DNA methylation and histone deacetylation. It can functionally recruit the nucleosome remodeling and deacetylase (NuRD) complex to the CpG-methylated promoters of the genes, which will be silenced during the cell fate determination. This review focuses on the cell-specific functions of MBD2 isoforms (MBD2a, MBD2b, and MBD2c(t)) in cellular differentiation, reprogramming, and the immune system. Furthermore, the relevance between MBD2 and certain cancers was discussed for the first time in this paper. Thus, we provide a comprehensive review about the significance of MBD2 in various cellular mechanisms.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"316"},"PeriodicalIF":2.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of FLT3 ligand on the gene expression of TIM-3, HIF1-α, and TNF-α in an acute myeloid leukemia cell line.","authors":"Fatemeh Heidari, Nazanin Atieh Kazemi-Sefat, Parisa Feizollahi, Sajjad Gerdabi, Ali Akbar Pourfathollah, Masoumeh Ebtekar","doi":"10.1007/s11033-025-10396-4","DOIUrl":"https://doi.org/10.1007/s11033-025-10396-4","url":null,"abstract":"<p><strong>Background: </strong>Acute Myeloid Leukemia (AML) pathogenesis is driven by the dysregulation of various cell signaling pathways, including the FMS-Like Tyrosine Kinase 3 (FLT3) pathway and its ligand (FLT3L). These pathways play a critical role in promoting cell survival, proliferation, and resistance to apoptosis, contributing to leukemogenesis. In this study, we investigated the effects of FLT3L on the expression of key genes associated with immune regulation, hypoxia, and inflammation-TIM-3, HIF-1α, and TNF-α-in the THP-1 cell line, a well-established model for AML research.</p><p><strong>Methods: </strong>THP-1 cells were cultured under standard conditions and treated with varying concentrations of FLT3L, alongside PMA as a positive control. Quantitative RT-PCR was employed to measure the expression levels of TIM-3, HIF-1α, and TNF-α genes after 48 h of treatment.</p><p><strong>Results: </strong>Our findings demonstrated that specific concentrations of FLT3L significantly upregulated the expression of TIM-3, HIF-1α, and TNF-α in THP-1 cells. This suggests that FLT3L not only influences cell proliferation and survival but also modulates pathways related to immune evasion, hypoxia adaptation, and inflammatory responses, which are hallmarks of leukemia progression.</p><p><strong>Conclusion: </strong>These results highlight the pivotal role of FLT3L in regulating the expression of genes associated with AML pathogenesis, particularly those involved in hypoxia (HIF-1α), immune checkpoint regulation (TIM-3), and inflammation (TNF-α). The findings underscore the potential of targeting the FLT3 pathway as a therapeutic strategy in AML. Further studies are warranted to elucidate the underlying molecular mechanisms and explore their clinical implications for improving patient outcomes.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"313"},"PeriodicalIF":2.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of curcumin on cyclosporine A-induced oxidative stress, autophagy, and apoptosis in rat heart.","authors":"Roya Naderi, Alireza Seyhani, Alireza Shirpoor, Adele Jafari, Kimia Eyvani","doi":"10.1007/s11033-025-10334-4","DOIUrl":"https://doi.org/10.1007/s11033-025-10334-4","url":null,"abstract":"<p><strong>Background: </strong>Cyclosporine A (CsA) is a powerful immunosuppressant commonly used as a prophylaxis on transplant. However, it is associated with serious effects, including cardiotoxicity. Curcumin is a bioactive compound known for its anti-oxidative, anti-inflammatory, and anti-apoptotic effects. So, the present study investigated the possible protective effect of curcumin on CsA-induced heart injury in rats, focusing on oxidative stress, autophagy, and apoptosis.</p><p><strong>Methods: </strong>A total of 32 male Wistar rats were divided into control, sham (drug solvent), CsA (30 mg/kg BW), and curcumin + CsA (40 mg/kg BW, 30 mg/kg BW, respectively) groups. After 4 weeks of treatment, the heart was isolated for molecular assays. Immunoblot detected oxidative and autophagic proteins NOX4, hsp-70, beclin-1, and LC3II. The amount of 8-OHdG was measured by ELISA and heart apoptosis was examined by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling staining (TUNEL).</p><p><strong>Results: </strong>At the molecular levels, CSA increased the expression of NOX-4, beclin-1, LC3b, and oHdG in heart tissue. In addition, the amount of apoptosis increased in the heart tissue. However, curcumin treatment improved heart injury by significantly downregulating NOX4, LC3b, and decreasing 8-OHdG. Also, curcumin significantly reduced the rate of myocardial apoptosis.</p><p><strong>Conclusion: </strong>To sum up, curcumin appears to protect against CsA-induced cardiotoxicity in rats by reducing oxidative activity, apoptosis, and regulating autophagy.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"310"},"PeriodicalIF":2.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lin28/let-7 axis in breast cancer.","authors":"P Shaik Syed Ali, Md Parwez Ahmad, K M Huria Parveen","doi":"10.1007/s11033-025-10413-6","DOIUrl":"https://doi.org/10.1007/s11033-025-10413-6","url":null,"abstract":"<p><p>Let-7 microRNAs are tumor suppressor microRNAs, and their reduced expression frequently occurs in various types of cancers, including breast cancer. A notable correlation exists between decreased let-7 microRNA levels and the overexpression of Lin28A and Lin28B, particularly in breast cancer cases with poor prognoses. Dysregulation of Wnt signaling significantly contributes to the upregulation of Lin28A and Lin28B in breast cancer. Both Lin28A and Lin28B operate from different cellular compartments to inhibit the biogenesis of let-7 microRNAs, which are essential for the post-transcriptional regulation of genes involved in key cellular functions such as proliferation, differentiation, and apoptosis. Decreased expression of let-7 microRNAs leads to the overexpression of oncogenes such as K-ras, C-myc, and SOX-2 in breast cancer. Overexpression of Lin28A associated with reduced let-7 microRNA levels is observed in estrogen receptor positive, estrogen receptor negative, and human epidermal growth factor receptor 2 positive breast cancers, whereas Lin28B overexpression with reduced let-7 microRNA levels occurs specifically in triple negative breast cancer. This review aims to dissect the molecular interplay between Lin28A, Lin28B, and let-7 microRNAs, elucidating their roles in breast carcinogenesis, metastasis, and the development of resistance to conventional treatments like radiation and chemotherapy. Additionally, the review addresses potential therapeutic avenues offered by let-7 microRNAs or their mimics, as well as Lin28A and Lin28B inhibitors, in the treatment of breast cancer.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"311"},"PeriodicalIF":2.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticancer potential of purified laccase enzyme from Trametes versicolor: specific cytotoxicity against thyroid and endometrial cancer cells.","authors":"Elif Kale Bakir, Asuman Deveci Ozkan, Gulay Erman, Semih Isik, Yonca Yuzugullu Karakus","doi":"10.1007/s11033-025-10416-3","DOIUrl":"https://doi.org/10.1007/s11033-025-10416-3","url":null,"abstract":"<p><strong>Background: </strong>Cancer is one of the leading causes of death worldwide, highlighting the need to develop novel therapeutic strategies that are more effective and have fewer side effects than conventional treatments. Enzymatic cancer therapy is a promising approach due to its high specificity and minimal toxicity. Among the various enzymes, laccase, a widely used biocatalyst, has shown significant potential for anti-cancer applications due to its proliferation inhibitory properties.</p><p><strong>Methods and results: </strong>In this study, the enzyme laccase from Trametes versicolor was purified by three-phase partitioning and then its cytotoxic, genotoxic and apoptotic effects on thyroid cancer (TT) and endometrial cancer (Ishikawa) cell lines were investigated. Laccase exhibited IC₅₀ values of 88.63 µM in TT cells and 1.68 µM in Ishikawa cells. The enzyme triggered apoptosis in Ishikawa cells and induced cell cycle arrest in S phase, while significantly increasing DNA damage in both cancer cell lines. Treatment with laccase led to downregulation of the anti-apoptotic gene Bcl-2 and upregulation of the pro-apoptotic gene Bax and the DNA damage repair genes Rad51 and ATM.</p><p><strong>Conclusions: </strong>Our results emphasize the specific cytotoxicity and molecular mechanisms underlying the anti-cancer effect of laccase and demonstrate that laccase is capable of selectively targeting cancer cells and causing apoptosis and DNA damage. This study demonstrates the potential of laccase as a novel enzymatic therapeutic for the treatment of thyroid and endometrial cancer and warrants further investigation into its clinical application and efficacy.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"312"},"PeriodicalIF":2.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Atypical pathogen community-acquired pneumonia: an analysis of clinical characteristics, drug treatment, and prognosis in the related patients.","authors":"Ying Yu, Minghui Li","doi":"10.1007/s11033-025-10382-w","DOIUrl":"https://doi.org/10.1007/s11033-025-10382-w","url":null,"abstract":"<p><strong>Introduction: </strong>Serious respiratory infections can occur in both in-hospital and out-of-hospital settings. These infections are known as community-acquired pneumonias (CAPs). Streptococcus pneumoniae and other microorganisms commonly cause atypical pneumonia. This study examined the clinical features, medication therapy, and prognosis of 85 cases of community-acquired pneumonia (CAP) caused by Mycoplasma pneumoniae (MPP) and Chlamydia psittaci(C. psittaci)neumoniae (CPP).</p><p><strong>Methods: </strong>A retrospective analysis was conducted at Shaoxing People's Hospital from July 2021 to August 2024, using targeted next-generation sequencing (tNGS) of bronchoalveolar lavage fluid (BALF). Patients were classified into the MPP group (54 patients) and the CPP group (31 patients). Compared with the control group, the CPP group had a significantly lower proportion of patients with a contact history of poultry and birds, a shorter length of hospital stay, and a lower percentage of severe pneumonia cases.</p><p><strong>Results: </strong>The MPP group demonstrated higher incidences of cough and sputum production; conversely, the occurrences of fever, fatigue, diminished appetite, and generalised myalgia were comparatively lower. The MPP group exhibited markedly diminished levels of neutrophils, C-reactive protein, procalcitonin, erythrocyte sedimentation rate, heparin-binding protein, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, direct bilirubin, pH, lactic acid, and D-dimer compared with the CPP group. In contrast, the MPP group had a markedly higher lymphocyte count, platelet count, albumin levels, as well as higher concentrations of blood sodium and blood chloride. The drug treatment regimens differed between the two groups, resulting in one unfavourable outcome within the MPP group.</p><p><strong>Conclusion: </strong>In summary, fatigue, fever, and reduced appetite are more prominent symptoms in patients with CPP, whereas cough and sputum production are the primary manifestations of MPP. Pleural effusion is more prevalent in patients with CPP, Additionally, these patients also have increased inflammatory responses and decreased immune function.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"309"},"PeriodicalIF":2.6,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}