Molecular Biology Reports最新文献_第4页

SARS-CoV-2 accessory proteins ORF3a and ORF6 alter the miRNome of human lung epithelial cells. SARS-CoV-2辅助蛋白ORF3a和ORF6改变人肺上皮细胞的miRNome

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-05-22 DOI: 10.1007/s11033-025-10596-y

Apoorva, Atul Kumar, Sankha Shubhra Chakrabarti, Sunit Kumar Singh

{"title":"SARS-CoV-2 accessory proteins ORF3a and ORF6 alter the miRNome of human lung epithelial cells.","authors":"Apoorva, Atul Kumar, Sankha Shubhra Chakrabarti, Sunit Kumar Singh","doi":"10.1007/s11033-025-10596-y","DOIUrl":"https://doi.org/10.1007/s11033-025-10596-y","url":null,"abstract":"Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of COVID-19. The accessory proteins of SARS-CoV-2 have been reported to attune host immune responses and viral pathogenicity. We have studied the effect of SARS-CoV-2 accessory proteins ORF3a and ORF6 on the expression pattern of miRNAs and their impact on cell signaling pathways in human lung epithelial cells.Methods and results: The miRNA expression profiling of human lung epithelial cells revealed a subset of 14 and 19 differentially expressed miRNAs (DEMs) in response to SARS-CoV-2 ORF3a and ORF6, respectively. Target prediction tools and subsequent bioinformatic analysis revealed the involvement of DEMs in key signaling pathways like PI3K/AKT, TNF, MAPK, TGF-β, and NF-κB, as a bystander effect of SARS-CoV-2 ORF3a and ORF6. The target genes were validated using real-time PCR and immunoblotting techniques. The results demonstrate that SARS-CoV-2 ORF3a and ORF6 exploit host cellular miRNAs such as hsa-miR-101-3p, hsa-miR-4455, hsa-miR-10b-5p, hsa-miR-940, and hsa-miR-4483, etc. to modulate the key cellular signaling pathways like NF-κB, TGF-β, Ras, IL-17, MAPK, and TNF signaling pathways.Conclusions: The present study demonstrates that SARS-CoV-2 ORF3a and ORF6 modulate the miRNA expression pattern in human lung epithelial cells. ORF3a exploits miRNAs to trigger a pro-inflammatory response, while ORF6 antagonizes IFN signaling via miRNA dysregulations to help SARS-CoV-2 in evading the host's immune response.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"494"},"PeriodicalIF":2.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the role of necroptosis in the immunological microenvironment of periodontitis. 探讨坏死性上睑下垂在牙周炎免疫微环境中的作用。

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-05-22 DOI: 10.1007/s11033-025-10589-x

Yuanwei Chen, Tairan Wang, Zhanglong Zheng, Zexin Ai, Jirui Jiang, Shengjiao Li

{"title":"Investigating the role of necroptosis in the immunological microenvironment of periodontitis.","authors":"Yuanwei Chen, Tairan Wang, Zhanglong Zheng, Zexin Ai, Jirui Jiang, Shengjiao Li","doi":"10.1007/s11033-025-10589-x","DOIUrl":"https://doi.org/10.1007/s11033-025-10589-x","url":null,"abstract":"Background: Extensive research has delved into the nexus between necroptosis and immunity, yet its impact on the immunological microenvironment of periodontitis remains elusive. Therefore, the study aims to elucidate the role of necroptosis in shaping this particular microenvironment.Results: We examined the differential expression of necroptosis genes in healthy and periodontitis samples, analyzing their correlations with infiltrating immunocytes, immune responses, and the human leukocyte antigen (HLA) gene. Distinct necroptosis-mediated expression patterns were identified, along with genes associated with the necroptosis phenotype. Notably, 37 necroptosis genes were dysregulated, leading to the development of a seven-necroptosis classifier that accurately distinguished periodontitis from healthy samples. The findings reveal a profound association between necroptosis and the immunological microenvironment, evidenced by the positive correlation between ZBP1 and MLKL expression with plasma cells, the negative correlation between TNFRSF1B and ZBP1 with resting dendritic cells, and the modulation of BCR signaling and TGF family receptor activity by ZBP1 and MLKL. Furthermore, we uncovered a positive correlation between ZBP1 and HLA-C expression and a negative correlation between HSPA4 and HLA-A expression. The analysis identified two distinct necroptosis expression patterns, each characterized by unique immune features. Among the 5272 genes associated with the necroptosis phenotype, 339 genes were linked to immunity, their biological functions centering on immunocyte regulation.Conclusion: This study underscores the significant role of necroptosis in shaping the immunological microenvironment of periodontitis, offering novel insights into the pathogenesis of this condition and paving the way for potential therapeutic strategies for periodontitis and its systemic comorbidities.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"491"},"PeriodicalIF":2.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between SIRT-1 and SERPINA4 gene polymorphisms and the risk of idiopathic nephrotic syndrome among Egyptian children. SIRT-1和SERPINA4基因多态性与埃及儿童特发性肾病综合征风险之间的关系

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-05-22 DOI: 10.1007/s11033-025-10568-2

Hind S AboShabaan, Hanan Mostafa El-Sayed, Mona A Abbas

{"title":"Association between SIRT-1 and SERPINA4 gene polymorphisms and the risk of idiopathic nephrotic syndrome among Egyptian children.","authors":"Hind S AboShabaan, Hanan Mostafa El-Sayed, Mona A Abbas","doi":"10.1007/s11033-025-10568-2","DOIUrl":"https://doi.org/10.1007/s11033-025-10568-2","url":null,"abstract":"Background: Idiopathic nephrotic syndrome (INS) is the primary cause of chronic glomerular dysfunction in children. Despite extensive research, its pathophysiology remains unclear, particularly in cases that are resistant to steroids.Aim: This research evaluated the impact of SIRT-1 (rs2273773) and SERPINA4 (rs2093266) gene variants on Egyptian children's susceptibility to INS and response to steroid therapy.Methods and results: Genetic polymorphisms of SIRT-1 (rs2273773) and SERPINA4 (rs2093266) were screened in 135 INS children and a similar number of healthy volunteers using real-time PCR. Concerning SIRT-1 rs2273773 genotypes, the cases showed markedly greater CT, TT genotypes, and T allele incidences than the reference group, which increased the risk of INS by 2.01-, 4.03-, and 1.88-folds, respectively. Regarding SERPINA4 rs2093266 genotypes, the cases exhibited notably higher GA, AA genotypes, and the A allele frequencies than controls, which enlarged the risk of INS by 3.1-, 5.47-, and 2.82-folds, respectively. The frequency of GA and AA genotypes and the presence of the A allele of SERPINA4 rs2093266 were considerably higher in steroid-resistant versus steroid-sensitive cases. The logistic regression model stated the serum creatinine, blood urea nitrogen, and the SERPINA4 rs2093266 polymorphism as independent contributors to the risk for steroid resistance in INS cases.Conclusion: SIRT-1 (rs2273773) and SERPINA4 (rs2093266) gene polymorphisms significantly attributed to the risk of developing INS in Egyptian children. Furthermore, the SERPINA4 (rs2093266) polymorphism has a strong correlation with steroid resistance, suggesting that it could be a target for treatment to avoid severe renal problems and adverse steroid effects.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"493"},"PeriodicalIF":2.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulation of ATP8B2 in atherosclerosis exacerbates foam cell-like pathological changes via impairing lysosomal membrane fusion. 动脉粥样硬化中ATP8B2的下调通过损害溶酶体膜融合而加剧泡沫细胞样病理改变。

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-05-22 DOI: 10.1007/s11033-025-10565-5

Rui Bu, Weihao Zhao, Rui Liang

{"title":"Downregulation of ATP8B2 in atherosclerosis exacerbates foam cell-like pathological changes via impairing lysosomal membrane fusion.","authors":"Rui Bu, Weihao Zhao, Rui Liang","doi":"10.1007/s11033-025-10565-5","DOIUrl":"https://doi.org/10.1007/s11033-025-10565-5","url":null,"abstract":"Background: Atherosclerosis, a major cause of global mortality, involves the transformation of macrophages into foam cells, which is a key pathological process. This study aims to elucidate the molecular mechanisms that contribute to foam cell formation and the progression of atherosclerosis.Methods and results: We performed a comprehensive bioinformatics analysis of transcriptome data to identify differentially expressed genes (DEGs) associated with atherosclerosis. Using the human acute monocytic leukemia cell line THP-1, we established in vitro models of macrophages and foam cells to simulate the atherosclerotic microenvironment. Functional studies were conducted using siRNA-mediated knockdown, real-time PCR, Western blotting, and immunofluorescence imaging. Our results showed that ATP8B2 was significantly down-regulated in atherosclerotic foam cells. The downregulation of ATP8B2 led to impaired lysosomal membrane fusion, evidenced by an increase in CD63-positive compartments without a change in CD63 protein levels. Additionally, under starvation conditions, there was a significant accumulation of autophagosomes, indicating a defect in the autophagy-lysosomal pathway.Conclusions: This study, for the first time, demonstrates that the downregulation of ATP8B2 exacerbates atherosclerosis by disrupting lysosomal membrane fusion, leading to lipid accumulation and foam cell formation. These findings provide novel insights into the pathogenesis of atherosclerosis and suggest that ATP8B2 could be a potential therapeutic target for the prevention or treatment of this disease.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"485"},"PeriodicalIF":2.6,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144120294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic and molecular characterization of a recurrent SPTAN1 mutation causing SPG91. 引起SPG91的复发性SPTAN1突变的表型和分子特征。

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-05-21 DOI: 10.1007/s11033-025-10582-4

Shih-Chun Lan, Ming-Der Perng, Yung-Yee Chang, Ying-Fa Chen, Min-Yu Lan

{"title":"Phenotypic and molecular characterization of a recurrent SPTAN1 mutation causing SPG91.","authors":"Shih-Chun Lan, Ming-Der Perng, Yung-Yee Chang, Ying-Fa Chen, Min-Yu Lan","doi":"10.1007/s11033-025-10582-4","DOIUrl":"10.1007/s11033-025-10582-4","url":null,"abstract":"Background: Spectrins are ubiquitous cytoskeleton proteins found in all metazoan cells. αII-spectrin, encoded by SPTAN1, is the pivotal protein responsible for organization of the axonal cytoskeleton. Monoallelic SPTAN1 mutations cause various inherited neurological diseases, including spastic paraplegia 91 (SPG91), a type of hereditary spastic paraplegia (HSP).Methods and results: We reported two patients with SPG91 caused by the SPTAN1 mutation c.55 C > T (p.Arg19Trp), who presented with lower limb spasticity and polyneuropathy. An analysis of the patients reported in the literature in addition to the present patients revealed that SPTAN1 p.Arg19Trp was specific for an HSP phenotype, with 35% of the combined patients with sensory‒motor polyneuropathy and 30% with cerebellar ataxia. In computational simulations, this variant was predicted to perturb the stability of αII/β spectrin heterotetramerization but did not destabilize the tetramerization domain of αII-spectrin.Conclusions: Our findings on genotype‒phenotype correlations and genetic effects on molecular characteristics may provide important insights into the exploration of αII-spectrin-related neurological diseases.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"476"},"PeriodicalIF":2.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MNS blood group system in Tunisian blood donors: common and rare alleles. 突尼斯献血者的MNS血型系统：常见和罕见等位基因。

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-05-21 DOI: 10.1007/s11033-025-10552-w

Mohamed Hichem Sellami, Eya Ghazouani, Sondess Hadj Fredj, Hamida Ferchichi, Manel Chaabane, Houda Kaabi, Taieb Messaoud, Slama Hmida

{"title":"The MNS blood group system in Tunisian blood donors: common and rare alleles.","authors":"Mohamed Hichem Sellami, Eya Ghazouani, Sondess Hadj Fredj, Hamida Ferchichi, Manel Chaabane, Houda Kaabi, Taieb Messaoud, Slama Hmida","doi":"10.1007/s11033-025-10552-w","DOIUrl":"10.1007/s11033-025-10552-w","url":null,"abstract":"Background: The MNS blood group system is highly complex and characterized by the presence of major polymorphic antigens, namely MNS1/MNS2 and MNS3/MNS4. This study aims to examine the genetic basis of the common MNS antigens in Tunisian blood donors and discussed their importance in transfusion immunology.Methods and results: A group of 229 Tunisian blood donors were genotyped for the major alleles GYPA*01, GYPA*02, GYPB*03 and GYPB*04 using the SSP-PCR method. A specific GYPA region was sequenced in some subjects to examine if there is a rare allele. The statistical analysis was done using the HaploView Software. The molecular analysis showed that 4 blood donors are probably carrier of a rare GYPA allele. Furthermore, it appeared that the GYPA*01 and GYPB*04 alleles; and the GYPA*01-GYPB*04 haplotype are predominant in this series. As for the phenotype, the study showed that the MNS:1,2,-3,4 type is the most frequent in recruited blood donors.Conclusions: Overall, the study showed a balanced distribution of the targeted alleles. Furthermore, the observed frequencies appeared to be in harmony with that reported in other populations. Considering the importance of these findings, we think that this work will be an important contribution to the field of public health and epidemiology.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"474"},"PeriodicalIF":2.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the role of mitochondrial dysfunction and aging in COVID-19-Related neurological complications. 探讨线粒体功能障碍和衰老在covid -19相关神经系统并发症中的作用。

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-05-21 DOI: 10.1007/s11033-025-10586-0

Prajakta Hingole, Priya Saha, Sourav Das, Chayanika Gundu, Ashutosh Kumar

{"title":"Exploring the role of mitochondrial dysfunction and aging in COVID-19-Related neurological complications.","authors":"Prajakta Hingole, Priya Saha, Sourav Das, Chayanika Gundu, Ashutosh Kumar","doi":"10.1007/s11033-025-10586-0","DOIUrl":"10.1007/s11033-025-10586-0","url":null,"abstract":"The COVID-19 pandemic, caused by SARS-CoV-2, posed a tremendous challenge to healthcare systems globally. Severe COVID-19 infection was reported to be associated with altered immunometabolism and cytokine storms, contributing to poor clinical outcomes and in many cases resulting in mortality. Despite promising preclinical results, many drugs have failed to show efficacy in clinical trials, highlighting the need for novel approaches to combat the virus and its severe manifestations. Mitochondria, crucial for aerobic respiration, play a pivotal role in modulating immunometabolism and neuronal function, making their compromised capability as central pathological mechanism contributing to the development of neurological complications in COVID-19. Dysregulated mitochondrial dynamics can lead to uncontrolled immune responses, underscoring the importance of mitochondrial regulation in shaping clinical outcomes. Aging further accelerates mitochondrial dysfunction, compounding immune dysregulation and neurodegeneration, making older adults particularly vulnerable to severe COVID-19 and its neurological sequelae. COVID-19 infection impairs mitochondrial oxidative phosphorylation, contributing to the long-term neurological complications associated with the disease. Additionally, recent reports also suggest that up to 30% of COVID-19 patients experience lingering neurological issues, thereby highlighting the critical need for further research into mitochondrial pathways to mitigate long-tern neurological consequences of Covid-19. This review examines the role of mitochondrial dysfunction in COVID-19-induced neurological complications, its connection to aging, and potential biomarkers for clinical diagnostics. It also discusses therapeutic strategies aimed at maintaining mitochondrial integrity to improve COVID-19 outcomes.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"479"},"PeriodicalIF":2.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inhibitory effects of tetraselmis algae extract on cervical cancer cell proliferation: a molecular and cellular approach. 四瓣藻提取物对宫颈癌细胞增殖的抑制作用：分子和细胞途径。

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-05-21 DOI: 10.1007/s11033-025-10551-x

Abbas Asoudeh-Fard, Fatemeh Faezi Sis, Babak Rahmani, Mana Kamranjam, Fatemeh Soltanmohammadi, Mohammad Zareian Jahromi, Asghar Parsai

{"title":"Inhibitory effects of tetraselmis algae extract on cervical cancer cell proliferation: a molecular and cellular approach.","authors":"Abbas Asoudeh-Fard, Fatemeh Faezi Sis, Babak Rahmani, Mana Kamranjam, Fatemeh Soltanmohammadi, Mohammad Zareian Jahromi, Asghar Parsai","doi":"10.1007/s11033-025-10551-x","DOIUrl":"10.1007/s11033-025-10551-x","url":null,"abstract":"Background: Cervical cancer is the leading type of primary malignancy in the uterus, causing significant morbidity and mortality, particularly in developing countries. Traditional treatments often result in severe side effects and recurrence, highlighting the need for safer alternatives. This study investigates the anticancer potential of Tetraselmis suecica, a green microalga known for its bioactive compounds.Methods: HeLa cervical cancer cells and HUVEC were treated with varying concentrations of T. suecica (25, 50, 75, and 100 mg/ml) for 24 h. Cell viability was assessed using the MTT assay, and gene expression related to apoptosis (Bax, Bcl-2, Caspases-3, -8, -9, PTEN, and AKT) was evaluated via real-time PCR for HeLa cells. Apoptosis was quantified using Annexin-V/PI double-staining for HeLa cells as well. The effect on HUVEC cells was assessed only by the MTT assay.Results: Treatment with T. suecica significantly reduced HeLa cell viability to 25% at 75 mg/ml and increased the expression of pro-apoptotic genes while decreasing anti-apoptotic markers. Apoptotic cell populations rose markedly, indicating that T. suecica induces apoptosis through both intrinsic and extrinsic pathways. However, no significant effect on cell viability or apoptosis was observed in HUVEC cells, suggesting that T. suecica selectively targets cancer cells without affecting normal endothelial cells.Conclusions: T. suecica exhibits promising anticancer properties and may serve as a novel therapeutic agent for cervical cancer, warranting further research into its mechanisms and therapeutic applications. The selective activity on cancer cells and lack of toxicity to normal cells such as HUVECs further supports its potential for targeted cancer therapy.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"473"},"PeriodicalIF":2.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of differentially expressed genes and pathways in the post-ovulatory ampulla of cyclic pigs through a transcriptomics approach. 通过转录组学方法鉴定循环猪排卵后壶腹的差异表达基因和途径。

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-05-21 DOI: 10.1007/s11033-025-10605-0

Jaya Bharati, Satish Kumar, Bijoy Mukut Buragohain, Diptesh Das, Salam Jayachitra Devi, N H Mohan, Vivek Kumar Gupta

{"title":"Identification of differentially expressed genes and pathways in the post-ovulatory ampulla of cyclic pigs through a transcriptomics approach.","authors":"Jaya Bharati, Satish Kumar, Bijoy Mukut Buragohain, Diptesh Das, Salam Jayachitra Devi, N H Mohan, Vivek Kumar Gupta","doi":"10.1007/s11033-025-10605-0","DOIUrl":"10.1007/s11033-025-10605-0","url":null,"abstract":"Background: Information on global transcriptomic changes in the porcine ampulla after ovulation is crucial for understanding of oviductal physiology at the molecular level. The objective of the present study was to investigate the differentially expressed genes (DEGs) and signalling pathways regulating the functionality of ampulla in pigs post-ovulation.Methods and results: The RNA-sequencing of the post-ovulatory ampulla (POA) and early luteal ampulla (ELA) tissues was conducted using Illumina NextSeq2000. The R package NOISeq was used to obtain significantly differentially expressed genes (DEGs) with the probability of differential expression (1-FDR) value ≥ 0.95 and log2 fold change (log2FC) ≥ 1, which revealed 817 DEGs (657 up- and 160 down-regulated) in the POA vs. ELA group comparison. These DEGs were functionally annotated with various gene ontology terms like sterol biosynthetic process, growth, cell migration, and Reactome pathways like signal transduction, metabolism, and cell cycle, indicating key role of these molecular events in POA. The WNT, TNFR2 non-canonical NF-kB, and hedgehog signalling pathways along with the activation of the immune system process, were enriched in the POA vs. ELA group, which indicates their role in cell-cell interactions and cell fate determination in remodelling the oviductal microenvironment during transition from estrogen to progesterone domination. The highly connected upregulated hub genes ESR1, RAD51, YARS1, TYMS and CDK2 can be regarded as key regulatory factors in synchronizing the changes in POA at the molecular level in the oviduct.Conclusion: The present study revealed several DEGs, signalling pathways and novel modulatory factors associated with the ampullary physiology during early embryonic development in the POA, which may influence fertility and litter size in pigs.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"481"},"PeriodicalIF":2.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of thrombomodulin in pterygium: implications for inflammation and disease progression. 血栓调节蛋白在翼状胬肉中的表达：对炎症和疾病进展的影响

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-05-21 DOI: 10.1007/s11033-025-10588-y

Yu-Kuei Lee, Chun-Chieh Lai, I-Chen Peng, Yi-Hsun Huang

{"title":"Expression of thrombomodulin in pterygium: implications for inflammation and disease progression.","authors":"Yu-Kuei Lee, Chun-Chieh Lai, I-Chen Peng, Yi-Hsun Huang","doi":"10.1007/s11033-025-10588-y","DOIUrl":"10.1007/s11033-025-10588-y","url":null,"abstract":"Background: Pterygium is a chronic inflammatory condition of conjunctiva. Thrombomodulin (TM) is a glycoprotein involved in the regulation of inflammation. This study investigated TM expression in primary and recurrent pterygium compared to normal conjunctiva, along with its role in pterygium pathogenesis and potential as a therapeutic target for inflammation control.Methods and results: Pterygium (10 primary, 10 recurrent) and normal conjunctiva specimens were collected from 20 patients who underwent pterygium excision. TM expression was analyzed using immunofluorescence, western blotting, and real-time quantitative polymerase chain reaction (RT-PCR). Inflammatory markers, including interleukin-6 (IL-6), high mobility group box 1 (HMGB1), vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), and matrix metalloproteinase-1 (MMP-1), were measured. The results showed significantly lower TM expression in pterygium tissues (p < 0.01), with higher TM levels in the head region compared to the body, suggesting a localized inflammatory response. Additionally, macrophage marker F4/80 and neutrophil marker NIMP-R14 were elevated in pterygium tissues. Western blot and RT-PCR confirmed significantly reduced TM expression (p < 0.0001) in primary and recurrent pterygium, with recurrent cases showing even lower levels (p < 0.05). Elevated IL-6, HMGB1, VEGF, bFGF, and MMP-1 levels suggest a strong association between TM downregulation and increased inflammation.Conclusions: TM downregulation in pterygium (particularly in recurrent cases) may contribute to chronic inflammation and disease progression. Upregulation of TM at the pterygium head may represent a localized protective response against inflammation. TM supplementation should be explored as a novel therapeutic strategy to mitigate inflammation in pterygium.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"477"},"PeriodicalIF":2.6,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144111385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0