Molecular Biology Reports最新文献

{"title":"Investigation the immunotherapeutic potential of miR-4477a targeting PD-1/PD-L1 in breast cancer cell line using a CD8⁺ co-culture model.","authors":"Yasin Tülüce, Sedat Köstekci, Fuat Karakuş, Ahmet Yasin Keleş, Merve Tunçyürekli","doi":"10.1007/s11033-025-10435-0","DOIUrl":"https://doi.org/10.1007/s11033-025-10435-0","url":null,"abstract":"<p><strong>Background: </strong>In the present study, we investigated the immunotherapeutic and anticancer activities of microRNA-4477a (miR-4477a) as a PD-L1 inhibitor in breast cancer cells (MCF-7).</p><p><strong>Methods: </strong>To this end, a series of analytical procedures were conducted, including bioinformatic analysis, RT-PCR analysis, PD-L1 ELISA, in vitro co-culture analysis, cytotoxicity assays, cell migration assays, and colony formation assays, with the objective of determining the anticancer activity of the compound in question.</p><p><strong>Results: </strong>The results demonstrated that miR-4477a can bind to three distinct regions of PD-L1 mRNA with high scores (94%, 88% and 80%), effectively targeting and suppressing the crucial regulatory pathways of cancer cells. In vitro studies demonstrated that a 25 nM dose of miR-4477a caused relatively high cytotoxicity in the MCF-7 cell line, suppressed PD-L1 gene expression, and decreased sPD-L1 protein levels, strongly inhibited cell migration, and significantly reduced colony formation. The in vitro co-culture analysis revealed that cancer cells were unable to evade the surveillance and cytotoxic activity of T cells (CD8⁺) due to the blockade of PD-L1 expression by miR-4477a.</p><p><strong>Conclusions: </strong>In conclusion, miRNA-4477a has the capacity to regulate immune responses in breast cancer cells and may therefore be a promising candidate for use in cancer immunotherapy as a therapeutic agent.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"326"},"PeriodicalIF":2.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of nanocurcumin on immune-related factors in the ankylosing spondylitis patients: a double-blind, randomized, placebo-controlled clinical trial.","authors":"Halmat M Jaafar, Dana Muhammad Hamad Ameen, Talar Ahmad Merza Mohammad, Aziz Muzafar Jafaar","doi":"10.1007/s11033-025-10397-3","DOIUrl":"https://doi.org/10.1007/s11033-025-10397-3","url":null,"abstract":"<p><strong>Background: </strong>A rheumatic condition characterized by inflammation and increased bone mass at the primary sites of inflammation is called ankylosing spondylitis (AS). The pathophysiology of AS has been linked to Th17 and Regulatory T (Treg) cells, as well as the immunological and miRNA variables that are associated with them. This study looked at how immunological and miRNA variables in AS patients' peripheral blood (PB) were affected by nanocurcumin.</p><p><strong>Methods: </strong>For four months, 30 patients with AS were part of the test group, who got nanocurcumin daily, and 30 patients in the control group, who received a placebo. Using flow cytometry, the frequency of Th17 and Treg was determined. Real-time polymerase chain reaction was used to measure the expression levels of HIF1A, various related microRNAs (miRNAs; miR-18a, miR-206, and miR-30a), and CD39 with CD73 in Patients PBMCs. An enzyme-linked immunosorbent assay was also used to measure the amounts of factors and cytokines secreted in serum.</p><p><strong>Results: </strong>Following treatment with nanocurcumin, patients with AS experienced a large rise in Treg cells and a decrease in Th17 cells. The RT-PCR results showed that while miR-18a was dramatically upregulated after nanocurcumin treatment, the expression of miR-206 and miR-30a was significantly downregulated. Additionally, compared to the control group, the nanocurcumin-treated group had lower levels of HIF-1alpha production and higher levels of IGF-1, TGF-β, CD39, and CD73.</p><p><strong>Conclusion: </strong>According to the findings, Th17 and Treg cell dysregulation, along with other immunological factors and miRNAs in PB, affects the progression of AS. Nanocurcumin therapy has the potential to control these variables, as well as Th17 and Treg cells, and may, therefore, help treat AS and other autoimmune disorders.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"324"},"PeriodicalIF":2.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosmarinic acid: a promising agent for male rats' renal protection against ischemia/reperfusion injury.","authors":"Gholampour Firouzeh, Moghbeli Hanza Samira, Karimi Zeinab","doi":"10.1007/s11033-025-10422-5","DOIUrl":"https://doi.org/10.1007/s11033-025-10422-5","url":null,"abstract":"<p><strong>Background: </strong>This study investigated the potential anti-inflammatory and antioxidant renoprotective effects of rosmarinic acid, a naturally occurring phenylpropanoid, against renal damage and dysfunction following renal ischemia/reperfusion (I/R) in male rats.</p><p><strong>Methods: </strong>The rats were divided into four separate groups (n = 7 per group): sham (1 ml 1% DMSO, i.p.), sham + RA (rosmarinic acid, 100 mg/kg in 1 ml 1% DMSO, i.p.), I/R (bilateral renal ischemia for 45 min followed by 24 h reperfusion), I/R + RA (rosmarinic acid, 100 mg/kg in 1 ml 1% DMSO, i.p. once, 30 min prior to ischemia). After the 24-hour reperfusion period, kidney tissue samples, blood, and 24-hour urine were taken.</p><p><strong>Results: </strong>In contrast to the sham and sham + RA groups, IR injury (IRI) resulted in renal dysfunction (increased fractional excretion of sodium and decreased creatinine clearance), raised malondialdehyde levels, enhanced TLR4 (Toll-like receptor 4), NFĸB (nuclear factor-ĸB) and TNF-α (tumor necrosis factor-alpha) gene expression levels, and histological lesions in kidney tissue Administration of rosmarinic acid attenuated each change.</p><p><strong>Conclusions: </strong>Rosmarinic acid protects the kidney against IRI by reducing inflammation and oxidative stress. Rosmarinic acid's anti-inflammatory and antioxidant properties most likely play an important role in addressing functional problems and protecting the kidney from IRI.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"325"},"PeriodicalIF":2.6,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skeletal muscle atrophy induced by aging and disuse atrophy are strongly associated with the upregulation of the endoplasmic stress protein CHOP in rats.","authors":"J Max Michel, Joshua S Godwin, Nathan R Kerr, Thomas E Childs, Frank W Booth, C Brooks Mobley, David C Hughes, Michael D Roberts","doi":"10.1007/s11033-025-10415-4","DOIUrl":"10.1007/s11033-025-10415-4","url":null,"abstract":"<p><strong>Background: </strong>While canonical anabolic and proteolytic pathways have been well examined in the context of skeletal muscle proteostasis, the roles of endoplasmic reticulum stress (ERS) and the induced unfolded protein response (UPR) are underappreciated. Thus, we aimed to determine whether aging and/or disuse atrophy in rats altered skeletal muscle ERS/UPR markers.</p><p><strong>Methods and results: </strong>Soleus (SOL) and plantaris (PLT) muscles of 3-month-old (mo), 6 mo, 12 mo, 18 mo, and 24 mo rats (9-10 per group, 48 in total) were analyzed for UPR proteins with further analysis performed on the protein CHOP. The gastrocnemius muscles of 4 mo rats that had undergone hindlimb immobilization (HLI, n = 12) or sham casting (CTL, n = 12) were analyzed for similar targets as well as more extensive CHOP-related targets. CHOP protein was greater in the PLT and SOL of 18 and 24 mo rats versus other age groups (P < 0.05). Moreover, negative correlations existed between CHOP expression and normalized PLT (R=-0.702, P < 0.001) and SOL (R=-0.658, P < 0.001) muscle weights in all rats analyzed at different ages. CHOP protein expression was also greater in the gastrocnemius of HLI versus CTL rats (P < 0.001), and a negative correlation existed between CHOP protein expression and normalized muscle weights in these rats (R=-0.814, P < 0.001). Nuclear CHOP protein levels (P < 0.010) and genes transcriptionally regulated by CHOP were also greater in HLI versus CTL rats (P < 0.001) implicating transcriptional activity of CHOP is elevated during disuse atrophy.</p><p><strong>Conclusions: </strong>CHOP is operative during aging- and disuse-induced skeletal muscle atrophy in rodents, and more research is needed to determine if CHOP is a key mechanistic driver of these processes.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"322"},"PeriodicalIF":2.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11919930/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacteriological profile of diabetic foot ulcers and analysis of serum meteorin levels.","authors":"Esra Erdogan, Azize Yetisgen, Lezzan Keskin, Elif Seren Tanriverdi, Isilay Gokce Benk Ugur","doi":"10.1007/s11033-025-10427-0","DOIUrl":"https://doi.org/10.1007/s11033-025-10427-0","url":null,"abstract":"<p><strong>Objective: </strong>With the increase in the incidence of diabetes mellitus (DM) in recent years, diabetic foot ulcers (DFU), which are common and serious chronic complications of diabetes, have also become widespread. DFU is highly associated with a significant deterioration in quality of life as well as increased morbidity and mortality. Meteorin is a potent neurotrophic growth factor and shows antiangiogenic, antihyperalgesic, antinociceptive and neuroprotective effects. This study aimed to determine the possible relationship between meteorin, diabetes and diabetic foot ulcer by comparing the serum meteorin levels of healthy control group, DM patients and patients with diabetic foot ulcers.</p><p><strong>Methods: </strong>Our study included a total of 62 diabetic patients, 31 of whom had DFU, and 29 healthy individuals as a control group. Meteorin levels of the participants were measured using ELISA method in serum samples. Other laboratory and epidemiological data of the patients were obtained from the hospital database.</p><p><strong>Results: </strong>In the wound cultures taken from patients with DFU, the most commonly isolated bacteria were Staphylococcus aureus and Pseudomonas aeruginosa. Serum meteorin levels were found to be statistically significantly higher in diabetic patients as compared to the healthy control group, and among diabetic patients, those with DFU had significantly higher levels compared to those without DFU. A positive significant correlation was found between meteorin level and age, HbA1c, WBC, urea, sedimentation, CRP and ferritin.</p><p><strong>Conclusion: </strong>The results of our study, aimed at better understanding the biological functions and potential clinical applications of meteorin, suggest that meteorin could potentially be used as a biomarker for the development of DFU.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"323"},"PeriodicalIF":2.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modifications of RNA in cancer: a comprehensive review.","authors":"Shalu Ranga, Ritu Yadav, Meenakshi Chauhan, Ravindresh Chhabra, Parul Ahuja, Nikita Balhara","doi":"10.1007/s11033-025-10419-0","DOIUrl":"https://doi.org/10.1007/s11033-025-10419-0","url":null,"abstract":"<p><p>RNA modifications play essential roles in post-transcriptional gene regulation and have emerged as significant contributors to cancer biology. Major chemical modifications of RNA include N6-methyladenosine (m⁶A), 5-methylcytosine (m⁵C), N1-methyladenosine (m¹A), pseudouridine (ψ), and N7-methylguanosine (m⁷G). Their dynamic regulation highlights their roles in gene expression modulation, RNA stability, and translation. Advanced high-throughput detection methods, ranging from liquid chromatography-mass spectrometry and high-performance liquid chromatography to next-generation sequencing (NGS) and nanopore direct RNA sequencing, have enabled detailed studies of RNA modifications in cancer cells. Aberrant RNA modifications are associated with the dysregulation of tumor suppressor genes and oncogenes, influencing cancer progression, therapy resistance, and immune evasion. Emerging research suggests the therapeutic potential of targeting RNA-modifying enzymes and their inhibitors in cancer treatment. This review compiles and analyzes the latest findings on RNA modifications, presenting an in-depth discussion of the diverse chemical alterations that occur in RNA and their profound implications in cancer biology. It integrates fundamental principles with cutting-edge research, offering a holistic perspective on how RNA modifications influence gene expression, tumor progression, and therapeutic resistance. It emphasizes the need for further studies to elucidate the complex roles of RNA modifications in cancer, as well as the potential for multimodality therapeutic strategies that exploit the dynamic and reversible nature of these epitranscriptomic marks. It also attempts to highlight the challenges, gaps, and limitations of RNA modifications in cancer that should be tackled before their functional implications. Understanding the interplay between RNA modifications, cancer pathways, and their inhibitors will be crucial for developing promising RNA-based therapeutic approaches to cancer and personalized medicine strategies.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"321"},"PeriodicalIF":2.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of furin in the severity of COVID-19 infection via effects on miR-20b and miR-106a.","authors":"Ismail Mahmoud, Amr E Ahmed, Olfat Shaker","doi":"10.1007/s11033-025-10340-6","DOIUrl":"https://doi.org/10.1007/s11033-025-10340-6","url":null,"abstract":"<p><strong>Background: </strong>Since 2019, COVID-19 and its mutants have been among the fiercest epidemic viruses. Coronavirus is still affecting the world and will continue through its various mutants, the closest example of which is the XEC mutant. Vaccines are currently available to prevent coronavirus infections. However, the currently approved treatments after infection, especially for severely infected patients, are still limited, and they are not suitable for everyone. Many studies have investigated the ability of furin to repair coronavirus viral proteins, and other studies have shown how important miRNAs are for controlling gene expression.</p><p><strong>Aim of work: </strong>This work aims to clarify the role of furin and the possibility of alleviating the burden of viral infection with COVID-19 and its mutations via effects on miR-20b and miR-106a.</p><p><strong>Patients and methods: </strong>We collected blood samples from 40 controls and 50 patients. Each patient provided approximately 3 ml of blood, which was separated for measuring furin by ELISA and extracting RNA for real-time PCR for the relative quantification of miRNAs.</p><p><strong>Results: </strong>The serum levels of Furin and miR-106 were considerably greater in the COVID-19 group than in the control group; however, the level of miR-20b was considerably greater in the control group than in the patients group.</p><p><strong>Conclusion: </strong>These data suggest that furin and miR-20b concentrations could be beneficial in therapeutic approaches against COVID-19.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"320"},"PeriodicalIF":2.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Identification of a novel alternate promoter element in the PheST Operon of Escherichia coli.","authors":"Praveen Belagal","doi":"10.1007/s11033-025-10429-y","DOIUrl":"https://doi.org/10.1007/s11033-025-10429-y","url":null,"abstract":"","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"318"},"PeriodicalIF":2.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of the sRNA ncBCG427 on mycobacterial stress adaptation.","authors":"Kailun Zhang, Zejin Du, Zijian Wang, Yingyu Chen, Aizhen Guo","doi":"10.1007/s11033-025-10354-0","DOIUrl":"https://doi.org/10.1007/s11033-025-10354-0","url":null,"abstract":"<p><strong>Background: </strong>Mycobacterium tuberculosis complex (MTBC) comprises the primary pathogens responsible for tuberculosis in humans and animals, including the virulent Mycobacterium tuberculosis (M. tb) and Mycobacterium bovis (M. bovis). As a group of intracellular bacteria, MTBC has developed intricate mechanisms for infection and survival within host cells. Among these mechanisms, small non-coding RNAs (sRNAs) play essential roles in regulating post-transcriptional pathways that may aid in stress adaptation in mycobacteria. In our previous research, we identified a novel sRNA, ncBCG427, in BCG, the attenuated strain of M. bovis. This sRNA is differentially expressed under various stress conditions, and homologs exist in both M. tb and M. bovis.</p><p><strong>Method and results: </strong>To explore the regulatory capabilities of ncBCG427 on bacterial stress adaptation in the original BCG strain, we constructed overexpression (BCG_ncBCG427) and control (BCG_Vector) strains. Our findings demonstrated that ncBCG427 significantly enhanced BCG survival under carbon starvation and acid stress conditions, while it resulted in decreased survival under iron starvation conditions. Transcriptomic analyses revealed that overexpression of ncBCG427 substantially altered gene expression profiles in these three stress environments, with differentially expressed genes (DEGs) in the same pathways enriched for different stressors. Notably, we identified 22 DEGs that responded to two or three stress conditions, implicating their involvement in pathways related to the sulfur relay system, ribosome function, folate biosynthesis, and the biosynthesis of cofactors. This suggested that these genes may served as key regulators in ncBCG427-mediated stress adaptation. Furthermore, quantitative PCR (qPCR) was employed to verify the expression levels of 16 DEGs in BCG, with 14 of them matching homologous genes in M. tb. We then created a ncBCG427 overexpression strain in M. tb (MTB_ncBCG427) alongside a control strain (MTB_Vector). Interestingly, 4 of the 14 genes displayed consistent expression trends in both M. tb and BCG under different stress conditions.</p><p><strong>Conclusion: </strong>In conclusion, our study underscored the significant role of the sRNA ncBCG427 in enhancing mycobacterial survival under stress conditions and proposed potential target genes for further exploration into the mechanisms of stress adaptation in mycobacteria.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"317"},"PeriodicalIF":2.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic-induced gut dysbiosis: unraveling the gut-heart axis and its impact on cardiovascular health.","authors":"Navpreet Kaur, Pankaj Kumar, Mahadev Dhami, Khadga Raj Aran","doi":"10.1007/s11033-025-10425-2","DOIUrl":"https://doi.org/10.1007/s11033-025-10425-2","url":null,"abstract":"<p><p>Cardiovascular diseases (CVDs) remain the major cause of morbidity and mortality amongst people of all ages across the world. Research suggests that the initiation and progression of CVDs are associated with antibiotic-induced gut dysbiosis. Antibiotics are primarily intended to be used to treat bacterial infections, which can alter gut microbiota (GM) composition, by lowering the abundance of beneficial bacteria, like Firmicutes, Bacteroidetes, and increasing the profusion of Enterobacteriaceae, leading to harm on gut health. Additionally, it reduces short-chain fatty acids (SCFAs) and bile acid metabolism, increases trimethylamine N-oxide (TMAO) production, intestinal permeability allowing lipopolysaccharide (LPS) and TMAO into systemic circulation. SCFAs play a key role in lipid metabolism, inflammation, and strengthening of the intestinal barrier, and participate in CVDs through FFAR2 and FFAR3 receptors, whereas dysbiosis reduces SCFAs levels and worsens these effects. TMAO enhances oxidative stress, inflammation, endothelial dysfunction, and cholesterol dysregulation, thus worsening CVDs. Furthermore, LPS develops systemic inflammation, insulin resistance, and endothelial dysfunction by activating the NF-κB pathway. Dysbiosis also affects bile acid synthesis, disrupting lipid and glucose metabolism, further participating in the progression of CVDs. This article aims to explore the role of gut dysbiosis in various CVDs, including congenital heart disease, hypertension, valvular heart disease, coronary heart disease, and heart failure. Furthermore, this article aims to bridge the knowledge gap regarding the gut-heart axis by exploring how antibiotics alter the gut microbiota homeostasis, further contributing to the development of CVDs and therapeutic interventions that reduce cardiovascular risks and restore the gut microbiota homeostasis.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"319"},"PeriodicalIF":2.6,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}