Molecular Biology最新文献_第4页

Microtubule-Associated Protein 9 Is a Potential Tumor Suppressor That Is Inactivated by Methylation in Cervical Cancer 微管相关蛋白 9 是一种潜在的肿瘤抑制因子，在宫颈癌中因甲基化而失活

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-05-02 DOI: 10.1134/s0026893324700304

P. Li, F. Wang, Y. Y. Gao, W. Zhang, Y. Zhang

{"title":"Microtubule-Associated Protein 9 Is a Potential Tumor Suppressor That Is Inactivated by Methylation in Cervical Cancer","authors":"P. Li, F. Wang, Y. Y. Gao, W. Zhang, Y. Zhang","doi":"10.1134/s0026893324700304","DOIUrl":"https://doi.org/10.1134/s0026893324700304","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>Cervical cancer is a malignant disease that seriously affects women’s health, and the cause of cervical cancer is complex. Microtubule-associated protein 9 (MAP9) localizes to the mitotic spindle and it plays an important role in spindle assembly and centrosome integrity maintenance. The expression and function of MAP9 is unclear in cervical cancer. This study aims to explore the relationship between DNA methylation of MAP9 and cervical cancer, and the function of MAP9 in cervical cancer. qRT-PCR was used to detect the transcriptional expression of MAP9 in cervical cancer tissues and cell lines. The methylation status of MAP9 promoter was determined by bisulfite sequencing PCR (BSP). The DNA methyltransferase inhibitor, 5-Aza-CdR, was used to inhibit the activity of methyltransferase. Then, the cell proliferation was performed by CCK-8 assay after the transfection of MAP9 plasmids. Furthermore, the cell cycle and apoptosis were performed by flow cytometry. It was found that MAP9 was down-regulated both in cervical cancer tissues and cell lines. The promoter and the first exon region of MAP9 was hypermethylated and its mRNA expression could be restored after the treatment of 5-Aza-CdR. Ectopic expression of MAP9 could inhibit cell proliferation and cell cycle, without effecting the cell apoptosis. The low expression of MAP9 in cervical cancer dues to its hypermethylation and acts a potential tumor suppressor gene. MAP9 might be a novel biomarker in cervical cancer.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"38 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140885136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

P3H4 Regulates Apoptosis and Autophagy of Breast Cancer Cells via BCL-2/BAX/Caspase-3 and AMPK/mTOR/ULK1 Signaling Pathways P3H4 通过 BCL-2/BAX/Caspase-3 和 AMPK/mTOR/ULK1 信号通路调控乳腺癌细胞的凋亡和自噬作用

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-05-02 DOI: 10.1134/s0026893324700237

T. Wang, M. M. Li, Z. Dong, D. M. Zhu

{"title":"P3H4 Regulates Apoptosis and Autophagy of Breast Cancer Cells via BCL-2/BAX/Caspase-3 and AMPK/mTOR/ULK1 Signaling Pathways","authors":"T. Wang, M. M. Li, Z. Dong, D. M. Zhu","doi":"10.1134/s0026893324700237","DOIUrl":"https://doi.org/10.1134/s0026893324700237","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The role of prolyl 3-hydroxylase family member 4 (P3H4, or SC65) in breast cancer and the molecular mechanisms in which this protein was involved were investigated. For this purpose, microchips with cancerous and paracancerous tissues collected from 56 patients with breast cancer were constructed. The P3H4 protein expression was evaluated by immunohistochemistry. Cell lines with decreased and increased P3H4 expression were selected and divided into five groups: P3H4 overexpression and corresponding negative control, P3H4 knockout groups #1 and #2 and corresponding negative control. CCK8 assay, colony formation test, immunoblotting, scratch test, transwell test and flow cytometry were used to determine the relevant cell functions. P3H4 expression was higher in breast cancer cells than in paracancerous tissue. Compared with corresponding negative control, proliferative activity of the cells was inhibited at 24, 48 and 72 h, migration activity and invasion ability of the cells were reduced, autophagy and apoptosis in the cells were increased in P3H4 knockout groups #1 and #2. P3H4 knockout promoted apoptosis of breast cancer cells and inhibited their proliferation, migration, and invasion by activating the BCL-2/BAX/Caspase-3 and AMPK/mTOR pathways. P3H4 knockout promoted apparently autophagy by activating the AMPK/mTOR/ULK1 pathway. However, P3H4 overexpression could promote the proliferation, migration and invasion of breast cancer cells, and inhibited apoptosis and autophagy of mammary gland adenocarcinoma cells MDA-MB-231.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"109 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140885191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ginkgo biloba Leaf Polysaccharide Induces Autophagy and Modulates the Expression of Apoptosis Markers in Hepatocellular Carcinoma Cells 银杏叶多糖诱导自噬并调节肝细胞癌细胞凋亡标志物的表达

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-05-02 DOI: 10.1134/s0026893324700328

K. Li, Z. F. Yu, K. X. Zhang, Z. H. Li, X. C. Liu, B. Y. Li, Y. X. Feng, K. F. Wei, Z. G. Yan

引用次数: 0

Cytotoxicity Studies of 5-Arylaminouracil Derivatives 5-Arylaminouracil 衍生物的细胞毒性研究

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-04-09 DOI: 10.1134/s0026893324020079

V. A. Kezin, E. S. Matyugina, S. A. Surzhikov, M. S. Novikov, A. A. Maslova, I. L. Karpenko, A. V. Ivanov, S. N. Kochetkov, A. L. Khandazhinskaya

{"title":"Cytotoxicity Studies of 5-Arylaminouracil Derivatives","authors":"V. A. Kezin, E. S. Matyugina, S. A. Surzhikov, M. S. Novikov, A. A. Maslova, I. L. Karpenko, A. V. Ivanov, S. N. Kochetkov, A. L. Khandazhinskaya","doi":"10.1134/s0026893324020079","DOIUrl":"https://doi.org/10.1134/s0026893324020079","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>We have previously shown that 5-arylaminouracil derivatives can inhibit HIV-1, herpesviruses, mycobacteria, and other pathogens through various mechanisms. The purpose of this study was to evaluate the potential of 5-arylaminouracils and their derivatives against leukemia, neuroblastoma, and glial brain tumors. 5-Aminouracils with various substituents and their 5'-norcabocyclic and ribo derivatives were screened for cytotoxicity against two neuroblastoma cell lines (SH-SY5Y and IMR-32), K-562 lymphoblastic cells, HL-60 promyeoloblastic cells, and low-passage variants of well-differentiated glioblastoma multiforme (GBM5522 and GBM6138). Cytotoxicity assessment by the standard MTT test showed that most of the compounds lack significant toxicity towards the above cells. However, 5-(4-isopropylphenylamine)uracil and 5‑(4-tert-butylphenylamine)uracil exhibited a dose-dependent toxic effect towards the GBM6138 cell line with half-maximal inhibitory concentrations (IC50) of 9 and 2.3 μM, respectively. Antitumor activity was for the first time demonstrated for compounds of this type and can serve as a starting point for further research.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"14 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Bioinformatics Method for Identification of Human Proteases Active against Viral Envelope Glycoproteins: A Case Study on the SARS-CoV-2 Spike Protein 鉴定对病毒包膜糖蛋白有活性的人类蛋白酶的生物信息学方法：关于 SARS-CoV-2 Spike 蛋白的案例研究

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-04-09 DOI: 10.1134/s0026893324020122

E. V. Matveev, G. V. Ponomarev, M. D. Kazanov

{"title":"A Bioinformatics Method for Identification of Human Proteases Active against Viral Envelope Glycoproteins: A Case Study on the SARS-CoV-2 Spike Protein","authors":"E. V. Matveev, G. V. Ponomarev, M. D. Kazanov","doi":"10.1134/s0026893324020122","DOIUrl":"https://doi.org/10.1134/s0026893324020122","url":null,"abstract":"Abstract—Many viruses, including SARS-CoV-2, the coronavirus responsible for the COVID-19 pandemic, enter host cells through a process of cell-viral membrane fusion that is activated by proteolytic enzymes. Typically, these enzymes are host cell proteases. Identifying the proteases that activate the virus is not a simple task but is important for the development of new antiviral drugs. In this study, we developed a bioinformatics method for identifying proteases that can cleave viral envelope glycoproteins. The proposed approach involves the use of predictive models for the substrate specificity of human proteases and the application of a structural analysis method for predicting the vulnerability of protein regions to proteolysis based on their 3D structures. Specificity models were constructed for 169 human proteases using information on their known substrates. A previously developed method for structural analysis of potential proteolysis sites was applied in parallel with specificity models. Validation of the proposed approach was performed on the SARS-CoV-2 spike protein, whose proteolysis sites have been well studied.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"56 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bipolar Action of Inhibitor of Vasculogenic Mimicry on Gene Expression in Melanoma Cells 血管生成模拟抑制剂对黑色素瘤细胞基因表达的双极作用

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-04-09 DOI: 10.1134/s0026893324020055

N. A. Tchurikov, A. A. Vartanian, E. S. Klushevskaya, I. R. Alembekov, A. N. Kretova, V. R. Chechetkin, G. I. Kravatskaya, V. S. Kosorukov, Y. V. Kravatsky

引用次数: 0

Method of Inducible Knockdown of Essential Genes in OSC Cell Culture of Drosophila melanogaster 在黑腹果蝇 OSC 细胞培养中诱导性敲除重要基因的方法

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-04-09 DOI: 10.1134/s0026893324020110

S. V. Marfina, E. A. Mikhaleva, N. V. Akulenko, S. S. Ryazansky

引用次数: 0

The Oral Microbiome in the Development of Oral Cancer 口腔癌发病过程中的口腔微生物组

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-04-09 DOI: 10.1134/s0026893324020092

E. S. Kolegova, A. A. Schegoleva, L. A. Kononova, E. V. Denisov

引用次数: 0

Changes in the Genome of the Tick-Borne Encephalitis Virus during Cultivation 蜱传脑炎病毒基因组在培养过程中的变化

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-04-09 DOI: 10.1134/s0026893324020146

V. A. Ternovoi, E. P. Ponomareva, E. V. Protopopova, N. L. Tupota, T. P. Mikryukova, V. B. Loktev

{"title":"Changes in the Genome of the Tick-Borne Encephalitis Virus during Cultivation","authors":"V. A. Ternovoi, E. P. Ponomareva, E. V. Protopopova, N. L. Tupota, T. P. Mikryukova, V. B. Loktev","doi":"10.1134/s0026893324020146","DOIUrl":"https://doi.org/10.1134/s0026893324020146","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Abstract</h3>The tick-borne encephalitis virus (TBEV) strain C11-13 (GenBank acc. no. OQ565596) of the Siberian genotype was previously isolated from the brain of a deceased person. TBEV C11-13 variants obtained at passages 3 and 8 in SPEV cells were inoculated into the brains of white mice for subsequent passages. Full genome sequences of all virus variants were analyzed by high-throughput sequencing. A total of 41 single nucleotide substitutions were found to occur mainly in the genes for the nonstructural proteins NS3 and NS5 (GenBank MF043953, OP902894, and OP902895), and 12 amino acid substitutions were identified in the deduced protein sequences. Reverse nucleotide and amino acid substitutions were detected after three passages through mouse brains. The substitutions restored the primary structures that were characteristic of the isolate C11-13 from a human patient and changed during the eight subsequent passages in SPEV cells. In addition, the 3′-untranslated region (3′-UTR) of the viral genome increased by 306 nt. The Y3 and Y2 3'-UTR elements were found to contain imperfect L and R repeats, which were probably associated with inhibition of cellular XRN1 RNase and thus involved in the formation of subgenomic flaviviral RNAs (sfRNAs). All TBEV variants showed high-level reproduction in both cell cultures and mouse brains. The genomic changes that occurred during successive passages of TBEV are most likely due to its significant genetic variability, which ensures its efficient reproduction in various hosts and its broad distribution in various climatic zones.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"298 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Regulation of Transcription by RNA Polymerase III Promotors in the Norm and Pathology 正常和病理情况下 RNA 聚合酶 III 启动子对转录的调控

IF 1.2 4区生物学

Molecular Biology Pub Date : 2024-04-09 DOI: 10.1134/s0026893324020134

A. M. Schwartz, K. A. Tatosyan, D. V. Stasenko, D. A. Kramerov

{"title":"Regulation of Transcription by RNA Polymerase III Promotors in the Norm and Pathology","authors":"A. M. Schwartz, K. A. Tatosyan, D. V. Stasenko, D. A. Kramerov","doi":"10.1134/s0026893324020134","DOIUrl":"https://doi.org/10.1134/s0026893324020134","url":null,"abstract":"Abstract—RNA polymerase III synthesizes a wide range of noncoding RNAs shorter than 400 nucleotides in length. These RNAs are involved in protein synthesis (tRNA, 5S rRNA, and 7SL RNA), maturation, and splicing of different types of RNA (RPR, MRP RNA, and U6 snRNA), regulation of transcription (7SK RNA), replication (Y RNA), and intracellular transport (vault RNA). BC200 and BC1 RNA genes are transcribed by RNA polymerase III in neurons only where these RNAs regulate protein synthesis. Mutations in the regulatory elements of the genes transcribed by RNA polymerase III as well as in transcription factors of this RNA polymerase are associated with the development of a number of diseases, primarily oncological and neurological. In this regard, the mechanisms of regulation of the expression of the genes containing various RNA polymerase III promoters were actively studied. This review describes the structural and functional classification of polymerase III promoters, as well as the factors involved in the regulation of promoters of different types. A number of examples demonstrate the role of the described factors in the pathogenesis of human diseases.","PeriodicalId":18734,"journal":{"name":"Molecular Biology","volume":"8 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140592367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0