Minerva endocrinology最新文献_第9页

Effect of spironolactone on pharmacological treatment of nonalcoholic fatty liver disease. 螺内酯对非酒精性脂肪肝药物治疗的影响。

IF 4.1

Minerva endocrinology Pub Date : 2023-09-01 Epub Date: 2021-10-20 DOI: 10.23736/S2724-6507.21.03564-8

Apostolis Papaefthymiou, Michael Doulberis, Kyriaki Karafyllidou, Eleftherios Chatzimichael, Georgia Deretzi, Aristomenis K Exadaktylos, Fotios Sampsonas, Athanasios Gelasakis, Spyros I Papamichos, Georgios Kotronis, Dimitra Gialamprinou, Elisabeth Vardaka, Stergios A Polyzos, Jannis Kountouras

{"title":"Effect of spironolactone on pharmacological treatment of nonalcoholic fatty liver disease.","authors":"Apostolis Papaefthymiou, Michael Doulberis, Kyriaki Karafyllidou, Eleftherios Chatzimichael, Georgia Deretzi, Aristomenis K Exadaktylos, Fotios Sampsonas, Athanasios Gelasakis, Spyros I Papamichos, Georgios Kotronis, Dimitra Gialamprinou, Elisabeth Vardaka, Stergios A Polyzos, Jannis Kountouras","doi":"10.23736/S2724-6507.21.03564-8","DOIUrl":"10.23736/S2724-6507.21.03564-8","url":null,"abstract":"Nonalcoholic fatty liver disease (NAFLD) was recently renamed to metabolic (dysfunction)-associated fatty liver disease (MAFLD) to better characterize its pathogenic origin. NAFLD represents, at least in western societies, a potential epidemic with raising prevalence. Its multifactorial pathogenesis is partially unraveled and till now there is no approved pharmacotherapy for NAFLD. A plethora of various choices are investigated in clinical trials, targeting an arsenal of different pathways and molecules. Since the mineralocorticoid receptor (MR) and renin-angiotensin-aldosterone system (RAAS) appear to be implicated in NAFLD, within this concise review, we focus on a rather classical and inexpensive pharmacological agent, spironolactone. We present the current lines of evidence of MR and RAAS-related preclinical models and human trials reporting an association with NAFLD. In conclusion, evidence about spironolactone of RAAS is commented, as potential future pharmacological management of NAFLD.","PeriodicalId":18690,"journal":{"name":"Minerva endocrinology","volume":"48 3","pages":"346-359"},"PeriodicalIF":4.1,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10346664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Results of semaglutide in patients older than 70 years, a real-world study of efficacy and safety. 西马鲁肽在70岁以上患者中的疗效和安全性的真实世界研究结果。

IF 4.1

Minerva endocrinology Pub Date : 2023-06-20 DOI: 10.23736/S2724-6507.23.03985-4

Maria D Garcia DE Lucas, Luis Perez-Belmonte, Beatriz Aviles-Bueno, Anabel Jimenez-Millan, Francisco Rivas Ruiz, José P Miramontes-González

{"title":"Results of semaglutide in patients older than 70 years, a real-world study of efficacy and safety.","authors":"Maria D Garcia DE Lucas, Luis Perez-Belmonte, Beatriz Aviles-Bueno, Anabel Jimenez-Millan, Francisco Rivas Ruiz, José P Miramontes-González","doi":"10.23736/S2724-6507.23.03985-4","DOIUrl":"https://doi.org/10.23736/S2724-6507.23.03985-4","url":null,"abstract":"Background: The aim of this study is to investigate the use of once-weekly semaglutide in a real population of patients with type 2 diabetes mellitus (T2DM) over 70 years in two Spanish hospitals.Methods: An observational, retrospective, and multicenter clinical study was designed. It included 60 patients with T2DM, with a mean age of 76.5 years, 63.3% women, and a mean of 15.5 years of evolution of T2DM, all managed in the outpatient clinical setting. The primary endpoint was the change in HbA<inf>1c</inf> from baseline to the end of the study. The secondary endpoints included changes in body weight and the proportion of patients achieving HbA<inf>1c</inf> <7.0% and body weight loss >5%.Results: After 12 months of follow-up, the reductions in HbA<inf>1c</inf> were -0.61±0.9% (P<0.0001) in the total cohort. Body weight reductions were -8.2±5.3 kg (P<0.0001). Overall, 67% reached the objective of an HbA<inf>1c</inf> level of <7%, and 73% achieved a weight loss of ≥5%.Conclusions: In routine clinical practice in Spain, the use of semaglutide once a week was associated with statistically significant and clinically relevant improvements in HbA<inf>1c</inf> and body weight in adults aged over 70 years with T2DM, without notable adverse effects, which supports real-world use.","PeriodicalId":18690,"journal":{"name":"Minerva endocrinology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9664268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of skin autofluorescence and carotid intima-media thickness in acromegaly patients. 肢端肥大症患者皮肤自身荧光与颈动脉内膜-中膜厚度的关系。

IF 4.1

Minerva endocrinology Pub Date : 2023-06-16 DOI: 10.23736/S2724-6507.23.03951-9

Meliha M Uygur, Mehmet Yaşar, Süreyya Ö Uygur, Dilek D Yazici, Dilek G Yavuz

{"title":"Association of skin autofluorescence and carotid intima-media thickness in acromegaly patients.","authors":"Meliha M Uygur, Mehmet Yaşar, Süreyya Ö Uygur, Dilek D Yazici, Dilek G Yavuz","doi":"10.23736/S2724-6507.23.03951-9","DOIUrl":"https://doi.org/10.23736/S2724-6507.23.03951-9","url":null,"abstract":"Background: The Pituitary Tumors Centers of Excellence (PTCOE) concept was established to provide a multimodal approach with careful management of comorbidities. Acromegaly, one of the main concerns of PTCOE per se, leads to increased mortality rates of which cardiovascular disease is an important cause. Increased skin autofluorescence (SAF) was shown to be associated with carotid intima-media thickness (CIMT), a well-established marker of atherosclerosis, and consequently cardiovascular complications. This study aimed to evaluate SAF and CIMT in association with anthropometric, clinical, and biochemical parameters in acromegaly patients and healthy controls.Methods: The study group included 138 acromegaly patients and 127 healthy controls from the Department of Endocrinology and Metabolism Disease, Marmara University Medical School. Growth hormone, insulin-like growth factor I, lipids, glucose, insulin levels were assessed. Advanced glycation end products (AGEs) were measured by the auto-fluorescence reader. CIMT was measured from the common carotid artery wall on B-mode ultrasound.Results: CIMT and SAF levels were significantly higher in the acromegaly group than the control group. There was a positive correlation between SAF and CIMT both in the total cohort and acromegaly patients. The presence of acromegaly, age, and SAF were the determining factors of CIMT in the whole study cohort.Conclusions: Our study is the first to examine the relationship between SAF and CIMT in acromegaly patients. We found higher CIMT and enhanced SAF in the acromegaly group compared to the control group with a significant positive correlation in between. The presence of acromegaly was related to increased SAF levels and CIMT. SAF was associated with CIMT in acromegaly patients. Implementation of CIMT and SAF evaluation in this clinical setting may improve cardiovascular complications, particularly in the PTCOE.","PeriodicalId":18690,"journal":{"name":"Minerva endocrinology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9631299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuropsychiatric symptoms, oral pigmentation and fever as revealing hints of autoimmune Addison's disease. 神经精神症状，口腔色素沉着和发热提示自身免疫性艾迪生病。

IF 4.1

Minerva endocrinology Pub Date : 2023-06-01 DOI: 10.23736/S2724-6507.21.03521-1

Raffaele Manna, Antonio Bianchi, Laura Gerardino, Clelia Cipolla, Donato Rigante, Raffaele Landolfi

引用次数: 1

Untargeted metabolomics analysis of serum and follicular fluid samples from women with polycystic ovary syndrome. 多囊卵巢综合征妇女血清和卵泡液样本的非靶向代谢组学分析

IF 4.1

Minerva endocrinology Pub Date : 2023-06-01 DOI: 10.23736/S2724-6507.20.03199-5

Wan-Li Xu, Guo-Yan Liu, Ning Zhang, Jian Ren, Xiu-Yang Li, Yu-Qiu Li, Yu Chen, Jia-Yi Liu

{"title":"Untargeted metabolomics analysis of serum and follicular fluid samples from women with polycystic ovary syndrome.","authors":"Wan-Li Xu, Guo-Yan Liu, Ning Zhang, Jian Ren, Xiu-Yang Li, Yu-Qiu Li, Yu Chen, Jia-Yi Liu","doi":"10.23736/S2724-6507.20.03199-5","DOIUrl":"https://doi.org/10.23736/S2724-6507.20.03199-5","url":null,"abstract":"Background: Polycystic ovary syndrome (PCOS) is a complex endocrine disorder with well-established metabolic abnormalities. In the present study, untargeted metabolomics technology was applied to analyze the serum and follicular fluid samples from women with polycystic ovary syndrome and healthy controls using 1H nuclear magnetic resonance (NMR).Methods: Seventy samples for PCOS analysis were collected in hospital of Shandong University of Traditional Chinese Medicine (Jinan, China), NMR was used as analytical technology and multivariate analysis was applied to analyze metabolomics difference in PCOS and healthy controls.Results: Significant metabolic differences were found in both serum and follicular fluid samples with orthogonal partial least-squares discriminant analysis (OPLS-DA). Three discriminated metabolites (1-Methylhistidine, threonine and Citrate) in both serum and follicular fluid were altered in PCOS patients. Abnormal energy metabolism, lipid metabolism and amino acid metabolism were detected in PCOS patients. Furthermore, more significantly changed amino acids were discovered in follicular fluid samples.Conclusions: Our findings would provide a resource for further investigations on metabolic disturbance in PCOS patients.","PeriodicalId":18690,"journal":{"name":"Minerva endocrinology","volume":"48 2","pages":"160-171"},"PeriodicalIF":4.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9463954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A clinical and in-silico study of microRNA-21 and growth differentiation factor-15 expression in pre-diabetes, type 2 diabetes and diabetic nephropathy. microRNA-21和生长分化因子-15在糖尿病前期、2型糖尿病和糖尿病肾病中的表达的临床和计算机研究

IF 4.1

Minerva endocrinology Pub Date : 2023-06-01 DOI: 10.23736/S2724-6507.22.03646-6

Riddhi G Agarwal, Manoj Khokhar, Purvi Purohit, Anupama Modi, Nitin K Bajpai, Gopal K Bohra, Mahendra K Garg, Praveen Sharma

{"title":"A clinical and in-silico study of microRNA-21 and growth differentiation factor-15 expression in pre-diabetes, type 2 diabetes and diabetic nephropathy.","authors":"Riddhi G Agarwal, Manoj Khokhar, Purvi Purohit, Anupama Modi, Nitin K Bajpai, Gopal K Bohra, Mahendra K Garg, Praveen Sharma","doi":"10.23736/S2724-6507.22.03646-6","DOIUrl":"https://doi.org/10.23736/S2724-6507.22.03646-6","url":null,"abstract":"Background: Diabetic nephropathy (DN), a microvascular complication associated with long-standing diabetes, is a major cause of the end-stage renal disease (ESRD). Our in-silico analysis indicates several enrichment analyses involved in glucose metabolism to be affected by GDF15 transcription factors.Methods: In-silico analysis was used to identify GDF15 and Insulin related protein-protein interaction (PPI) network and a common set of GDF15 regulating transcription factors by various databases. Common targeting miRNA of GDF15 regulating transcription factors were investigated in miRNet and TargetScan. Further, healthy controls (N.=30) and patients with pre-type-2 diabetes mellitus (pre-diabetes) (N.=30), T2DM (N.=30) and DN (N.=30) were included for analysis of routine biochemical tests, serum GDF15 levels by ELISA and to evaluate the Fold change expression (FCE) of circulating hsa-miR-21 by RT-PCR.Results: MicroRNA-21 was found to directly target GDF15 downregulating transcription factors KLF4, TP53, and CEBPB. A significant difference in the levels of serum GDF15 was observed in Pre-diabetes (708.56±76.37), T2DM (1528.87±140.75) and DN patients (10-fold higher; 5507.90±503.88) when compared to healthy controls (567.36±69.99). The FCE of circulating hsa-miR-21 was 6.19 (pre-diabetes), 8.22 (T2DM), 9.19 (DN), folds higher in cases as compared to controls, reflecting an increasing trend and several folds higher levels of hsa-miR-21 in patients.Conclusions: We suggest the potential of serum GDF15 and circulating-hsa-miR-21 to serve as clinically important biomarkers and therapeutic targets for controlling advancement of diabetes to DN.","PeriodicalId":18690,"journal":{"name":"Minerva endocrinology","volume":"48 2","pages":"172-185"},"PeriodicalIF":4.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9475564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Metformin improves survival in patients with concurrent diabetes and small cell lung cancer: a meta-analysis. 二甲双胍提高糖尿病合并小细胞肺癌患者的生存率:一项荟萃分析

IF 4.1

Minerva endocrinology Pub Date : 2023-06-01 DOI: 10.23736/S2724-6507.21.03289-2

Hua Fan, Shikun Bai, Xin Guan, Wen Ma, Yu Fu, Xuan Zhang, Lili Deng, Jing Tian

{"title":"Metformin improves survival in patients with concurrent diabetes and small cell lung cancer: a meta-analysis.","authors":"Hua Fan, Shikun Bai, Xin Guan, Wen Ma, Yu Fu, Xuan Zhang, Lili Deng, Jing Tian","doi":"10.23736/S2724-6507.21.03289-2","DOIUrl":"https://doi.org/10.23736/S2724-6507.21.03289-2","url":null,"abstract":"Introduction: To compare survival outcome among the patients with concurrent small cell lung cancer (SCLC) and diabetes mellitus (DM) using metformin or without metformin.Evidence acquisition: A systematic literature search for relevant studies up to Oct 2020 was conducted. Outcome of the included studies included overall survival (OS) or disease-free survival (DFS). HR values were pooled to estimate the effect of metformin on survival outcomes.Evidence synthesis: Six studies with 539 participants with both SCLC and diabetes were included in the analysis. The patients with metformin usage had significantly longer OS and DFS than those without metformin usage (OS: HR=0.72 [0.53-0.98], P=0.04; DFS: HR=0.59 [0.45-0.76], P<0.0001). The studies included were not significantly different in the two analyses by heterogeneity test. There was no obvious publication bias.Conclusions: Metformin may improve survival among patients with concurrent SCLC and DM. Further investigations especially randomized control trials are warranted, especially among the patients who do not have diabetes.","PeriodicalId":18690,"journal":{"name":"Minerva endocrinology","volume":"48 2","pages":"214-221"},"PeriodicalIF":4.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9525262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of tadalafil on skeletal muscle tissue: exploring interactions and novel mechanisms of action. 他达拉非对骨骼肌组织的影响:探索相互作用和新的作用机制。

IF 4.1

Minerva endocrinology Pub Date : 2023-06-01 DOI: 10.23736/S2724-6507.21.03698-8

Cristina Antinozzi, Emanuela A Greco, Paolo Sgrò, Ivan Dimauro, Antonio Aversa, Luigi DI Luigi

{"title":"Effects of tadalafil on skeletal muscle tissue: exploring interactions and novel mechanisms of action.","authors":"Cristina Antinozzi, Emanuela A Greco, Paolo Sgrò, Ivan Dimauro, Antonio Aversa, Luigi DI Luigi","doi":"10.23736/S2724-6507.21.03698-8","DOIUrl":"https://doi.org/10.23736/S2724-6507.21.03698-8","url":null,"abstract":"Beside its mechanical roles in controlling posture and locomotion, skeletal muscle system, the largest insulin and steroid hormones target tissue, plays a key role in influencing thermoregulation, secondary sexual characteristics, hormones metabolism, and glucose uptake and storage, as well as energetic metabolism. Indeed, in addition to insulin, several hormones influence the skeletal muscle metabolism/function and/or are influenced by skeletal muscles activity (i.e., physical exercise). Particularly, steroid hormones play a key role in modulating many biological processes in muscles, essential for overall muscle's function and homeostasis, both at rest and during all physical activities (i.e., physical exercise, muscular work). Phosphodiesterase type 5 (PDE5) is the enzyme engaged to hydrolyze cyclic guanosine monophosphate (cGMP) in inactive 5'-GMP form. Therefore, through the inhibition of this enzyme, the intracellular level of cGMP increases, and the cGMP-related cellular responses are prolonged. Different drugs inhibiting PDE5 (PDE5i) exist, and the commercially available PDE5i are sildenafil, vardenafil, tadalafil, and avanafil. The PDE5i tadalafil may influence cellular physiology and endocrine-metabolic pathways in skeletal muscles and exerts its functions both by activating the cell signaling linked to the insulin-related metabolic pathways and modulating the endocrine responses, protein catabolism and hormone-related anabolism/catabolism during and after physical exercise-related stress. Based on recent in-vivo and in-vitro findings, in this narrative review the aim was to summarize the available evidence describing the interactions between the PDE5i tadalafil and steroid hormones in skeletal muscle tissue and physical exercise adaptation, focusing our interest on their possible synergistic or competitive action(s) on muscle metabolism and function.","PeriodicalId":18690,"journal":{"name":"Minerva endocrinology","volume":"48 2","pages":"222-229"},"PeriodicalIF":4.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9463984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Effects of empagliflozin against indomethacin induced gastric mucosa. 恩格列净对吲哚美辛诱导的胃粘膜的影响。

IF 4.1

Minerva endocrinology Pub Date : 2023-06-01 DOI: 10.23736/S2724-6507.21.03425-4

Isilay Taskaldiran, Serife M Kuskonmaz, Pinar Celepli, Sema Hucumenoglu, Cemil Nural, Özcan Erel, Cavit Culha

{"title":"Effects of empagliflozin against indomethacin induced gastric mucosa.","authors":"Isilay Taskaldiran, Serife M Kuskonmaz, Pinar Celepli, Sema Hucumenoglu, Cemil Nural, Özcan Erel, Cavit Culha","doi":"10.23736/S2724-6507.21.03425-4","DOIUrl":"https://doi.org/10.23736/S2724-6507.21.03425-4","url":null,"abstract":"Background: Sodium-glucose cotransporter 2 (SGLT2) inhibitors (SGLT2i) are considered a new class antidiabetic agent, as well as lowering blood sugar, it has many positive effects. This study aimed to investigate the effects of SLGT2i on the gastric mucosa.Methods: We investigated the effects of empagliflozin on indomethacin-induced gastritis using 48 male Wistar Albino rats. We performed histopathological evaluations of gastric mucosa tissue. And we studied the levels of serum disulfide, native thiol, total thiol, and ischemia modified albumin, disulfide/native thiol ratio (SSSH), native thiol/total thiol percent ratio (SH total SH), and disulfide/total thiol percent ratio (SS total SH).Results: We found that empagliflozin increased mucin production in rat gastric mucosa. Besides, we observed milder inflammation findings and lower gastritis scores in the empagliflozin receiving groups than the placebo groups. Native thiol, total thiol, and disulfide levels were lower in the indomethacin-induced gastritis groups.Conclusions: This study is the first to investigate the effect of empagliflozin on the gastrointestinal tract in a rat model. We concluded that empagliflozin increased mucin production and revealed positive effects in an indomethacin-induced gastritis model.","PeriodicalId":18690,"journal":{"name":"Minerva endocrinology","volume":"48 2","pages":"186-193"},"PeriodicalIF":4.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9824495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Inhibition of neuropilin-1 improves non-alcoholic fatty liver disease in high-fat-diet induced obese mouse. 抑制neuropilin-1可改善高脂饮食诱导的肥胖小鼠的非酒精性脂肪性肝病。

IF 4.1

Minerva endocrinology Pub Date : 2023-06-01 DOI: 10.23736/S2724-6507.22.03895-7

Jian Zhou, Sian Xu, Yue Zhu, Xin Li, Ao Wang, Junfang Hu, Li Li, Yan Liu

{"title":"Inhibition of neuropilin-1 improves non-alcoholic fatty liver disease in high-fat-diet induced obese mouse.","authors":"Jian Zhou, Sian Xu, Yue Zhu, Xin Li, Ao Wang, Junfang Hu, Li Li, Yan Liu","doi":"10.23736/S2724-6507.22.03895-7","DOIUrl":"https://doi.org/10.23736/S2724-6507.22.03895-7","url":null,"abstract":"Background: Research findings indicate that neuropilin-1 plays a critical role in lipid metabolism and obesity-associated insulin resistance; on such a basis, the aim of this study was to explore the effects and working mechanism of neuropilin-1 inhibition on the non-alcoholic fatty liver (NAFLD) disease in high-fat-diet (HFD) induced obese mice.Methods: Firstly, the pcDNA3.1-NRP-1 recombinant plasmid containing neuropilin-1 (NRP1) gene and NRP1 RNA interference plasmid shRNA-NRP1 were successfully constructed. A total of 36 C57BL/6 mice were randomly assigned to 6 groups, blank group, control group, pcDNA3.1 injection group, pcDNA3.1-NRP-1 injection group, pGenesil-1.1 injection group and shRNA-NRP1 injection group. Expression of phospho-PI3K, phospho-AKT, phospho-mTOR and neuropilin-1 in liver was measured as well as body and liver weight, blood glucose, serum transaminases and lipid levels of the mice.Results: The weight and liver mass of HFD fed mice injected with pcDNA3.1-NRP-1 were significantly higher than those from the control group, but their body weight and liver mass decreased significantly after shRNA-NRP1 injection. The results also showed that neuropilin-1 expression can significantly influence the severity of hepatic steatosis in HFD fed mice, decreased serum FPG, LDL, AST, ALT levels and the expression of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and IL-6 mRNA. In addition, the Neuropilin-1 expression will also influence the p-PI3K, p-AKT and p-mTOR in mice.Conclusions: This study concluded that the inhibition of neuropilin-1 could improve NAFLD disease by decreasing body weight and reduce inflammation in HFD induced obese mice by modulating the PI3K/AKT/mTOR signaling pathway.","PeriodicalId":18690,"journal":{"name":"Minerva endocrinology","volume":"48 2","pages":"194-205"},"PeriodicalIF":4.1,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9476095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0