Molecular & general genetics : MGG最新文献_第2页

Analysis of genes involved in 6-deoxyhexose biosynthesis and transfer in Saccharopolyspora erythraea. 红糖多孢子菌6-脱氧己糖合成与转移相关基因分析。

Molecular & general genetics : MGG Pub Date : 2000-11-01 DOI: 10.1007/s004380000329

M Doumith, P Weingarten, U F Wehmeier, K Salah-Bey, B Benhamou, C Capdevila, J M Michel, W Piepersberg, M C Raynal

{"title":"Analysis of genes involved in 6-deoxyhexose biosynthesis and transfer in Saccharopolyspora erythraea.","authors":"M Doumith, P Weingarten, U F Wehmeier, K Salah-Bey, B Benhamou, C Capdevila, J M Michel, W Piepersberg, M C Raynal","doi":"10.1007/s004380000329","DOIUrl":"https://doi.org/10.1007/s004380000329","url":null,"abstract":"Glycosylation represents an attractive target for protein engineering of novel antibiotics, because specific attachment of one or more deoxysugars is required for the bioactivity of many antibiotic and antitumour polyketides. However, proper assessment of the potential of these enzymes for such combinatorial biosynthesis requires both more precise information on the enzymology of the pathways and also improved Escherichia coli-actinomycete shuttle vectors. New replicative vectors have been constructed and used to express independently the dnmU gene of Streptomyces peucetius and the eryBVII gene of Saccharopolyspora erythraea in an eryBVII deletion mutant of Sac. erythraea. Production of erythromycin A was obtained in both cases, showing that both proteins serve analogous functions in the biosynthetic pathways to dTDP-L-daunosamine and dTDP-L-mycarose, respectively. Over-expression of both proteins was also obtained in S. lividans, paving the way for protein purification and in vitro monitoring of enzyme activity. In a further set of experiments, the putative desosaminyltransferase of Sac. erythraea, EryCIII, was expressed in the picromycin producer Streptomyces sp. 20032, which also synthesises dTDP-D-desosamine. The substrate 3-alpha-mycarosylerythronolide B used for hybrid biosynthesis was found to be glycosylated to produce erythromycin D only when recombinant EryCIII was present, directly confirming the enzymatic role of EryCIII. This convenient plasmid expression system can be readily adapted to study the directed evolution of recombinant glycosyltransferases.","PeriodicalId":18636,"journal":{"name":"Molecular & general genetics : MGG","volume":"264 4","pages":"477-85"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s004380000329","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21950321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 104

Identification of the initiation codon for the atpB gene in Chlamydomonas chloroplasts excludes translation of a precursor form of the beta subunit of the ATP synthase. 衣藻叶绿体中atpB基因起始密码子的鉴定排除了ATP合成酶β亚基前体形式的翻译。

Molecular & general genetics : MGG Pub Date : 2000-11-01 DOI: 10.1007/s004380000332

B Rimbault, D Esposito, D Drapier, Y Choquet, D Stern, F A Wollman

引用次数: 23

Physiological evidence for differently regulated tryptophan-dependent pathways for indole-3-acetic acid synthesis in Azospirillum brasilense. 巴西氮螺旋藻合成吲哚-3-乙酸的色氨酸依赖途径不同调控的生理证据。

Molecular & general genetics : MGG Pub Date : 2000-11-01 DOI: 10.1007/s004380000340

R Carreño-Lopez, N Campos-Reales, C Elmerich, B E Baca

引用次数: 116

The Prr1 response regulator is essential for transcription of ste11+ and for sexual development in fission yeast. Prr1反应调节因子在裂变酵母中对ste11+的转录和性发育至关重要。

Molecular & general genetics : MGG Pub Date : 2000-11-01 DOI: 10.1007/s004380000305

R Ohmiya, H Yamada, C Kato, H Aiba, T Mizuno

{"title":"The Prr1 response regulator is essential for transcription of ste11+ and for sexual development in fission yeast.","authors":"R Ohmiya, H Yamada, C Kato, H Aiba, T Mizuno","doi":"10.1007/s004380000305","DOIUrl":"https://doi.org/10.1007/s004380000305","url":null,"abstract":"Schizosaccharomyces pombe expresses a putative transcription factor, named Prr1, which is intriguing in the sense that it contains a bacterial type of phospho-accepting receiver domain, preceded by a mammalian heat shock factor (HSF2)-like DNA-binding domain. The receiver domain is most probably involved in an as yet unidentified histidine-to-aspartate (His-to-Asp) phosphorelay pathway in S. pombe. In this study, the structure, function, and cellular localization of Prr1 were assessed in the context of oxidative stress and His-to-Asp phosphorelay. As the most intriguing result of this study, we found that Prr1 is essential not only for the expression of genes induced by oxidative stress (e.g., ctt1+ and trr1+), but also for the expression of ste11+, which in turn is responsible for the expression of a variety of genes required for sexual development. Accordingly, Prr1-deficient cells are not only hypersensitive to oxidative stress, but also severely defective in conjugation and/or spore formation. These results suggested that the transcription factor Prr1 plays a pivotal role in an as yet unknown signal transduction pathway that is implicated in sexual differentiation. These findings are discussed with special reference to the well-characterized transcription factors Pap1 and Atf1 of S. pombe.","PeriodicalId":18636,"journal":{"name":"Molecular & general genetics : MGG","volume":"264 4","pages":"441-51"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s004380000305","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21951548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

A putative mitochondrial ftsZ gene is present in the unicellular primitive red alga Cyanidioschyzon merolae. 推测线粒体ftsZ基因存在于单细胞原始红藻紫藻中。

Molecular & general genetics : MGG Pub Date : 2000-11-01 DOI: 10.1007/s004380000307

M Takahara, H Takahashi, S Matsunaga, S Miyagishima, H Takano, A Sakai, S Kawano, T Kuroiwa

引用次数: 103

Phylogenetic analysis of the functional domains of mariner-like element (MLE) transposases. 水手样元件(MLE)转座酶功能区系统发育分析。

Molecular & general genetics : MGG Pub Date : 2000-11-01 DOI: 10.1007/s004380000334

C Augé-Gouillon, H Notareschi-Leroy, P Abad, G Periquet, Y Bigot

引用次数: 19

Analysis of ENOD40 expression in alb1, a symbiotic mutant of Lotus japonicus that forms empty nodules with incompletely developed nodule vascular bundles. 形成空结节且结节维管束不完全发育的白莲共生突变体alb1中ENOD40的表达分析

Molecular & general genetics : MGG Pub Date : 2000-11-01 DOI: 10.1007/s004380000330

H Imaizumi-Anraku, H Kouchi, K Syono, S Akao, M Kawaguchi

{"title":"Analysis of ENOD40 expression in alb1, a symbiotic mutant of Lotus japonicus that forms empty nodules with incompletely developed nodule vascular bundles.","authors":"H Imaizumi-Anraku, H Kouchi, K Syono, S Akao, M Kawaguchi","doi":"10.1007/s004380000330","DOIUrl":"https://doi.org/10.1007/s004380000330","url":null,"abstract":"The alb1 mutant of Lotus japonicus (Ljsym74) forms empty nodules in which most of the bacteria remain in abnormally enlarged infection threads and fail to enter the host plant cells. The alb1 mutant was also found to be defective in differentiation of ramified nodule vascular bundles; only a single vascular bundle differentiates at the proximal end of the alb1 nodules and it fails to differentiate further. Histochemical analysis using fluorescein-conjugated wheat-germ agglutinin (F-WGA) indicated that the mutation in the ALB1 gene specifically affects the differentiation of vascular bundles in nodules. Analysis of nodulin gene expression revealed that the expression of an early nodulin gene, ENOD40, was very low in alb1 nodules. At early developmental stages of alb1 nodules, the pattern of ENOD40 transcription was essentially the same as that in wild-type nodules; transcripts were localized in dividing cortical cells and in the pericycle of the root stele opposite nodule primordia, as in wild-type nodules. However, mature alb1 nodules exhibited very weak or no expression of ENOD40 in the peripheral cells of the undeveloped nodule vascular bundle. The ENOD40 expression pattern in alb1 nodules is distinct from that in another ineffective mutant, fen1 (Ljsym76), in which ENOD40 expression persists prior to premature senescence. These findings lead us to speculate that ENOD40 may play a role in the differentiation of nodule vascular bundles.","PeriodicalId":18636,"journal":{"name":"Molecular & general genetics : MGG","volume":"264 4","pages":"402-10"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s004380000330","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21951543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

The MAK-V protein kinase regulates endocytosis in mouse. MAK-V蛋白激酶调节小鼠内吞作用。

Molecular & general genetics : MGG Pub Date : 2000-11-01 DOI: 10.1007/s004380000293

I V Korobko, E V Korobko, S L Kiselev

引用次数: 17

Allele-specific interactions between the yeast RFC1 and RFC5 genes suggest a basis for RFC subunit-subunit interactions. 酵母RFC1和RFC5基因之间的等位基因特异性相互作用提示了RFC亚基-亚基相互作用的基础。

Molecular & general genetics : MGG Pub Date : 2000-11-01 DOI: 10.1007/s004380000339

W Beckwith, M A McAlear

{"title":"Allele-specific interactions between the yeast RFC1 and RFC5 genes suggest a basis for RFC subunit-subunit interactions.","authors":"W Beckwith, M A McAlear","doi":"10.1007/s004380000339","DOIUrl":"https://doi.org/10.1007/s004380000339","url":null,"abstract":"Replication factor C (RFC) is an essential, multi-subunit ATPase that functions in DNA replication, DNA repair, and DNA metabolism-related checkpoints. In order to investigate how the individual RFC subunits contribute to these functions in vivo, we undertook a genetic analysis of RFC genes from budding yeast. We isolated and characterized mutations in the RFC5 gene that could suppress the cold-sensitive phenotype of rfc1-1 mutants. Analysis of the RFC5 suppressors revealed that they could not suppress the elongated telomere phenotype, the sensitivity to DNA damaging agents, or the mutator phenotype of rfc1-1 mutants. Unlike the checkpoint-defective rfc5-1 mutation, the RFC5 suppressor mutations did not interfere with the methylmethane sulfonate- or hydroxyurea-induced phosphorylation of Rad53p. The Rfc5p suppressor substitutions mapped to amino acid positions in the conserved RFC box motifs IV-VII. Comparisons of the structures of related RFC box-containing proteins suggest that these RFC motifs may function to coordinate interactions between neighboring subunits of multi-subunit ATPases.","PeriodicalId":18636,"journal":{"name":"Molecular & general genetics : MGG","volume":"264 4","pages":"378-91"},"PeriodicalIF":0.0,"publicationDate":"2000-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s004380000339","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21952253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Informational suppressor alleles of the eEF1A gene, fertility and cell degeneration in Podospora anserina. 鹅足孢子虫eEF1A基因的信息抑制等位基因、育性和细胞退化。

Molecular & general genetics : MGG Pub Date : 2000-10-01 DOI: 10.1007/s004380000337

P Silar, M Rossignol, R Tahar, Z Derhy, A Mazabraud

引用次数: 5