Menoufia Medical Journal最新文献_第3页

Evaluation of glycerol antiseptic effect on Gram positive and Gram negative bacteria 甘油对革兰氏阳性菌和革兰氏阴性菌防腐效果的评价

Menoufia Medical Journal Pub Date : 2023-10-05 DOI: 10.59204/2314-6788.1100

Asmaa S. Sleem, Samar S. Salman, Wafaa A. Shehata, Amal M. Dawoud

{"title":"Evaluation of glycerol antiseptic effect on Gram positive and Gram negative bacteria","authors":"Asmaa S. Sleem, Samar S. Salman, Wafaa A. Shehata, Amal M. Dawoud","doi":"10.59204/2314-6788.1100","DOIUrl":"https://doi.org/10.59204/2314-6788.1100","url":null,"abstract":"Objectives: To assess glycerol antibacterial activity against both Gram positive and negative microorganisms, detect impact of increasing glycerol concentration in bacterial cultures on extra/ intracellular Glycerol-3-phosphate (G3P) concentration, evaluate antiseptic efficacy of different glycerol/alcohol formulations and monitor HCWs tolerability to these formulations to recommend the best antiseptic preparation in vitro. Background: The progressively rising nosocomial infections have enforced the need for novel infection prevention modalities. Glycerol emerges as a significant and cheap antibacterial agent against both Gram positive and negative organisms without any toxic effects. Methodology: Different glycerol/ethanol formulations were evaluated for both bacterial inhibitory and bactericidal activity against selected bacteria (4 reference and 5 clinical strains). Effect of glycerol/ethanol combination was tested by checkerboard method. Colorimetric assay of extra and intracellular bacterial concentration of G3P was done. Skin tolerance of involved HCWs was evaluated against obtained bactericidal formulations including; 80% ethanol (E80)without glycerol (G0) as group (A), E80G1.45 (B), E80G20 (C)and E0G80 as group (D). Results: Microbial growth inhibition was detected at G40E40, G40E60, G1.45 E80 and G20 E80 for most strains. No visible growth (bactericidal effect) was detected with G80, G20 E80 and G1.45 E80. Glycerol/ethanol combination showed synergistic effect against Enterococcus faecalis and biofilm producing MRSA with additive effect against Proteus mirabilis, staphylococcus aureus. Acinetobacter baumannii displayed indifference. No antagonism was detected. G3P levels were positively correlated with added glycerol concentration. E80G20 achieved HCWs maximum tolerability (100%) followed by E0G80 (92.3%). Conclusion: E80G20 and E0G80 were superior to WHO recommended E80G1.45. However, more advanced studies are needed.","PeriodicalId":18558,"journal":{"name":"Menoufia Medical Journal","volume":"301 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135483927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic Approach and risk stratification of thrombocytosis: Integrating morphological, cytogenetic features and MPL gene mutation 血小板增多症的诊断方法和危险分层:整合形态学、细胞遗传学特征和MPL基因突变

Menoufia Medical Journal Pub Date : 2023-09-26 DOI: 10.59204/2314-6788.1053

Olfat M. Hendy, Dina A. Fouad, Shaimaa A. Pessar, Sara M. Aboagiza, Hanan M. Bedair

{"title":"Diagnostic Approach and risk stratification of thrombocytosis: Integrating morphological, cytogenetic features and MPL gene mutation","authors":"Olfat M. Hendy, Dina A. Fouad, Shaimaa A. Pessar, Sara M. Aboagiza, Hanan M. Bedair","doi":"10.59204/2314-6788.1053","DOIUrl":"https://doi.org/10.59204/2314-6788.1053","url":null,"abstract":"Objectives: To evaluate the integration of clinical, morphological, cytogenetic features and MPL gene mutation analysis in Egyptian patients with clonal thrombocytosis and correlate these finding to patient’s outcome. Background: Thrombocytosis can be caused by either reactive or autonomous processes as a result of genetic alterations that affect signaling pathway through gain-of-function activity of MPL (myeloproliferative leukemia virus), Janus kinase 2 (JAK 2), calreticulin (CALR). Methods: This study was conducted on 60 Egyptian patients having clonal thrombocytosis attending to clinical pathology department, Ain Shams University Hospitals during the period from August 2020 to December 2021. Complete blood count, LDH level and MPL gene mutation by Real-time PCR were measured. Results: JAK2 gene mutation was more prevalent than MPL gene mutation in our study (40% and 8.3% respectively). MPL mutation has an aggressive nature than JAK2 mutation as evident by; older age (68.80± 2.28 vs 55.5 ± 8.28 accordingly), more thrombotic manifestation (100% vs 58.3%), higher platelets count (1221.4 ± 309.70 vs 879.38 ± 215.98) and poor response to therapy (0% of MPL positive patients achieved complete response to therapy vs 41.7% for JAK2 positive patients) but with no marked morphological difference regarding megakaryocytes morphology. Conclusion: MPL W515L/K mutations present in 10.3% in essential thrombocythemia Egyptian patients, included in our current study. MPL gene mutation has a bad prognostic effect. Moreover, presence of coexisting JAK2 & MPL mutation have a synergistic bad effect to be evaluated in further larger studies.","PeriodicalId":18558,"journal":{"name":"Menoufia Medical Journal","volume":"94 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135719115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Mac-2-binding protein glycosylation isomer in assessment of liver fibrosis in patients with chronic hepatitis C virus receiving direct-acting antiviral agents mac -2结合蛋白糖基化异构体在接受直接作用抗病毒药物的慢性丙型肝炎患者肝纤维化评估中的作用

Menoufia Medical Journal Pub Date : 2023-09-08 DOI: 10.59204/2314-6788.1095

Mohsen Elkhayat, Ayman El Lehleh, Noran T. Aboelkhair, Mai Abozeid, Hadeer A. Shahin, Naglaa S. Elabd

引用次数: 0

Early and midterm outcomes of left anterior descending coronary artery reconstruction in extensive coronary artery atherosclerosis 广泛性冠状动脉粥样硬化左前降支重建的早期和中期预后

Menoufia Medical Journal Pub Date : 2023-08-31 DOI: 10.59204/2314-6788.1097

Hesham H. Ahmed, Khaled A. Sha’aban, A. Dokhan, Rafik F. Soliman, Mohammed G. Abdellatif

引用次数: 0

Assessment of Serum Copeptin Levels in Pediatric Patients with Sickle Cell Anemia 儿童镰状细胞性贫血患者血清Copeptin水平的评估

Menoufia Medical Journal Pub Date : 2023-08-28 DOI: 10.59204/2314-6788.1040

M. El-Hawy, A. Shehata, Basem Abd El-Fattah El-Gazzar, Ahmed Mostafa Ahmed Esmaiel, Mai El-Sayad Abd El-Hamid El-Saeedy

引用次数: 0

Combined Knee Arthroscopy with Medial Open Wedge High Tibial Osteotomy in Management of Medial Knee Joint Disorder of Adults Varus Knee 膝关节镜联合内侧开楔胫骨高位截骨术治疗成人膝内翻的内侧膝关节紊乱

Menoufia Medical Journal Pub Date : 2023-08-28 DOI: 10.59204/2314-6788.1056

E. E. Agroudy

引用次数: 0

Correlation between serum uric acid level and angiographic severity of coronary artery disease in patients with acute coronary syndrome 急性冠脉综合征患者血清尿酸水平与冠脉造影严重程度的相关性

Menoufia Medical Journal Pub Date : 2023-08-28 DOI: 10.59204/2314-6788.1093

Mohamed Yahia, Mustafa Ahmad, A. Elkersh

引用次数: 0

Phosphorus: The Forgotten Electrolyte in The Paediatric Intensive Care Unit 磷:儿科重症监护病房中被遗忘的电解质

Menoufia Medical Journal Pub Date : 2023-08-28 DOI: 10.59204/2314-6788.1082

Fady Mohamed ElGendy, Wafaa Abdelaziz Eladrosy, Muhammad Said El-Mekkawy

{"title":"Phosphorus: The Forgotten Electrolyte in The Paediatric Intensive Care Unit","authors":"Fady Mohamed ElGendy, Wafaa Abdelaziz Eladrosy, Muhammad Said El-Mekkawy","doi":"10.59204/2314-6788.1082","DOIUrl":"https://doi.org/10.59204/2314-6788.1082","url":null,"abstract":"Objective To evaluate prevalence of hypophosphatemia and hyperphosphatemia and their association with outcome among critically ill children. Background Phosphorus is essential element for various biological functions but data about serum phosphorus level abnormalities among critically ill children are limited. Patients and methods Prospective observational study including children admitted into Pediatric Intensive Care Unit (PICU). Serum phosphorus was measured on admission, day4, day8, and day12. Pediatric Sequential Organ Failure Assessment (pSOFA) score was calculated on admission. Hypophosphatemia and hyperphosphatemia risk factors were determined. Results Hypophosphatemia and hyperphosphatemia were generally non-severe. Hypophosphatemic patients had higher frequency of sepsis on admission compared with normophosphatemic patients (36.2% vs. 12.5%, p=0.019). Frequency of steroid use was higher among hypophosphatemic children (27.7% vs. 6.3%, p=0.017). Hypophosphatemia was associated with longer PICU stay (p=0.028) and hospitalacquired infections (p=0.022). pSOFA and frequencies of both sepsis and elevated serum creatinine were significantly higher among hyperphosphatemic compared with normophosphatemic patients. Hyperphosphatemia was associated with higher mechanical ventilation rate (p=0.018). No significant difference in mortality rate was found between hypophosphatemic, hyperphosphatemic, and normophosphatemic patients. However, phosphorus level on day8 was lower among non-survivors [median (interquartile range): 3.2 (2.3–4.7) vs. 3.9 (3.4–5.2), p=0.046]. Conclusion Phosphorus abnormalities, particularly hypophosphatemia, are common among critically ill children. Hyperphosphatemia was more likely to be found among patients with renal dysfunction, sepsis, and higher pSOFA score. Hypophosphatemia was associated with longer PICU stay, hospital-acquired infections, higher mechanical ventilation rate and pSOFA score.","PeriodicalId":18558,"journal":{"name":"Menoufia Medical Journal","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135033102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lateral Extra-articular Tenodesis Combined with Intra-articular ACL Reconstruction in Management of Anterolateral Knee Instability 外侧关节外肌腱固定术联合关节内前交叉韧带重建治疗膝关节前外侧不稳

Menoufia Medical Journal Pub Date : 2023-08-28 DOI: 10.59204/2314-6788.1058

E. E. Agroudy

引用次数: 0

Evaluation of Results of Percutaneous Acetabular Columns Fixation 经皮髋臼柱固定效果评价

Menoufia Medical Journal Pub Date : 2023-08-28 DOI: 10.59204/2314-6788.1059

E. E. Agroudy

引用次数: 0