Metabolomics最新文献

{"title":"Coronary artery disease is associated with particular change of serum metabolome: a case-control study.","authors":"Marcin Kondraciuk, Małgorzata Chlabicz, Jacek Jamiołkowski, Natalia Zieleniewska, Michał Ciborowski, Adrian Godlewski, Emilia Sawicka-Śmiarowska, Katarzyna Ptaszyńska, Magda Łapińska, Adam Krętowski, Karol A Kamiński","doi":"10.1007/s11306-025-02253-z","DOIUrl":"https://doi.org/10.1007/s11306-025-02253-z","url":null,"abstract":"<p><strong>Introduction: </strong>Cardiovascular disease (CVD) is a significant cause of mortality worldwide. Preventive programs are trying to reduce the burden of the disease. Recent advances in metabolomics profiling open a new avenue for developing complementary CVD evaluation strategies.</p><p><strong>Objectives: </strong>The aim of the study was to investigate whether a metabolomic profile can provide an additional characterisation of individuals with coronary artery disease (CAD).</p><p><strong>Methods: </strong>The study included 167 participants with CAD aged 41-79 years. A control group was formed of 166 individuals without CAD, gender- and age-matched to the study group. A total of 188 metabolites were profiled in serum by liquid chromatography-tandem mass spectrometry. After clearing the data, associations between 132 metabolites and CAD presence were analysed using multiple linear regression models.</p><p><strong>Results: </strong>We observed significant differences in serum metabolic profiles between analysed groups on various levels. However, a deeper analysis revealed sphingomyelin 41:1 (SM 41:1) as the main metabolite independently associated with CAD after correction for classical CV risk factors. Its concentration was lower in the CAD group (median 9.79 µmol/L, interquartile range (IQR) 7.92-12.23) compared to control one (median 13.60 µmol/L, IQR 11.30-16.15) (p < 0.001). Further analysis showed that SM 41:1 concentration was inversely correlated with CAD, current smoking, and hypertension; and positively associated with female gender and non-HDL level.</p><p><strong>Conclusions: </strong>CAD patients present lower plasma concentrations of SM 41:1 than healthy subjects. A better understanding of the biological function of sphingomyelin in CAD patients may help develop therapeutic approaches and risk stratification in this group.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 3","pages":"57"},"PeriodicalIF":3.5,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12031763/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methionine, threonine and glutamic acid adapted pathways in captive cheetahs on a glycine-supplemented diet.","authors":"Kathryn M van Boom, Tertius A Kohn, Adrian S W Tordiffe","doi":"10.1007/s11306-025-02243-1","DOIUrl":"https://doi.org/10.1007/s11306-025-02243-1","url":null,"abstract":"<p><strong>Background: </strong>Captive cheetahs are prone to a range of unusual diseases potentially linked to unnatural diets high in muscle meat and low in collagen-rich animal fibre. In the wild, cheetahs typically eat whole prey diets not easily replicated in a captive setting. Glycine is the most abundant amino acid in collagen with a key role in several metabolic pathways such as collagen biosynthesis. Several recent studies suggest that endogenous glycine production may be limited in several species.</p><p><strong>Objectives: </strong>Using untargeted ¹H- nuclear magnetic resonance, the metabolic changes in the urine and serum of 10 adult captive cheetahs on a glycine-supplemented diet were investigated.</p><p><strong>Methods: </strong>Cheetahs were fed either a meat only (control) or glycine-supplemented meat diet (30 g glycine per 1 kg meat) for four weeks, followed by a four-week cross-over. Urine and blood samples were collected at baseline and after each intervention.</p><p><strong>Results: </strong>A total of 151 and 60 metabolites were identified in the urine and serum, respectively. Specifically, dimethylsulphone, proline, fructose, dimethylamine, trimethylamine, pyroglutamic acid, 1,3-diaminopropane, dihydrothymine, methylmalonic acid and pimelic acid contributed to metabolome differences in the urine. In serum, glutamic acid, threonine, α-aminobutyric acid, glucose-6-phosphate, ethanolamine, methionine and propionic acid were highlighted. These metabolites play various metabolic roles in energy production, immune function, protein and collagen biosynthesis or as products of gut microbiome fermentation.</p><p><strong>Conclusion: </strong>Glycine supplementation influenced threonine sparing, pyrimidine biosynthesis pathways and bacterial fermentation products, although the implications of these findings on the health of captive cheetahs is unknown. Future studies should use a targeted approach to further elaborate on these pathways.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 3","pages":"56"},"PeriodicalIF":3.5,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12018600/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic and functional analyses of small molecules secreted by intestinal nematodes in the activation of epithelial tuft cells.","authors":"Marta Campillo Poveda, Stephan Löser, Victoria Gillan, Josh Richards, Claire Ciancia, Gavin Blackburn, Erin Kerr, Michael Barrett, Katie A Hildersley, Philippe Jay, Eileen Devaney, Tom N McNeilly, Collette Britton, Rick M Maizels","doi":"10.1007/s11306-025-02248-w","DOIUrl":"https://doi.org/10.1007/s11306-025-02248-w","url":null,"abstract":"<p><strong>Introduction: </strong>Intestinal helminth parasites trigger the host immune response through epithelial sensory tuft cells, but helminth-derived molecules that may activate tuft cells are poorly characterized.</p><p><strong>Objectives: </strong>The study aimed to identify small molecules released in vitro by two nematode parasites, that infect rodents (Nippostrongylus brasiliensis) and ruminants (Haemonchus contortus), and to test candidate ligands in an in vivo model of tuft cell differentiation.</p><p><strong>Methods: </strong>Small molecules were analyzed by hydrophilic interaction liquid chromatography (HILIC) of material released by adult parasites incubated in serum-free media, followed by mass spectrometry; selected molecules were administered to mice and tuft cell expansion enumerated after 5 days.</p><p><strong>Results: </strong>A range of different conditions (culture media, timing, oxygenation) were tested, and comparisons made between the conditions, and between the two nematode species at selected points. Common products across the conditions and species included carboxylic acids (malate, succinate), medium chain fatty acids (such as decanoic and undecanoic acids), purines (guanine, xanthine and their derivatives), and phosphocholine compounds. We selected 19 of the prominent molecules for in vivo testing by oral administration, including succinate, a known activator of tuft cell differentiation. Malate elicited a low but significant level of tuft cell expansion, while undecanoic acids with or without a bromine substitution were also able to induce significant differentiation comparable to succinate. Other molecules including phosphorylcholine had no effect.</p><p><strong>Conclusion: </strong>Multiple molecular species including decanoic and undecanoic acids released by helminths may contribute to activation of tuft cells in vivo.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 3","pages":"55"},"PeriodicalIF":3.5,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011944/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass spectrometry-based characterisation of the cardiac microtissue metabolome and lipidome.","authors":"Tara J Bowen, Andrew R Hall, Andrew D Southam, Ossama Edbali, Ralf J M Weber, Amanda Wilson, Amy Pointon, Mark R Viant","doi":"10.1007/s11306-025-02252-0","DOIUrl":"https://doi.org/10.1007/s11306-025-02252-0","url":null,"abstract":"<p><strong>Introduction: </strong>The use of large, non-sample specific metabolite reference libraries often results in high proportions of false positive annotations in untargeted metabolomics.</p><p><strong>Objective: </strong>This study aimed to measure and curate a library of polar metabolites and lipids present in cardiac microtissues.</p><p><strong>Results: </strong>Untargeted ultra-high performance liquid chromatography-coupled mass spectrometry measurements of cardiac microtissue intracellular extracts were annotated by comparison against four spectral databases and a retention time library. The annotations were combined to create a library of 313 polar metabolites and 1004 lipids.</p><p><strong>Conclusions: </strong>The curated library will facilitate higher confidence metabolite annotation in mass spectrometry-based untargeted metabolomics of cardiac microtissues.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 3","pages":"54"},"PeriodicalIF":3.5,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144034613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The causal association between circulating metabolites and Alzheimer's disease: a systematic review and meta-analysis of Mendelian randomization studies.","authors":"Yuxuan Wu, Fangying Chen, Tingting Zhang, Mengrong Miao, Mengxin Zhang, Jiaqiang Zhang, Enqiang Chang","doi":"10.1007/s11306-025-02242-2","DOIUrl":"https://doi.org/10.1007/s11306-025-02242-2","url":null,"abstract":"<p><strong>Introduction/objective: </strong>Some Mendelian randomization (MR) studies have found that there may be a genetic causal relationship between circulating metabolites and Alzheimer 's disease (AD), but the strength of evidence and the direction of association are not always consistent. In this study, a systematic review and meta-analysis of all the literature using MR methods to study the causal relationship between metabolites and AD was conducted to enhance the robustness and correlation of predicting genetic causality.</p><p><strong>Methods: </strong>We conducted a comprehensive review of Mendelian randomization (MR) studies which are within the timeframe of all years to 20 December 2023. Circulating metabolites were considered as the exposure factor, and AD served as the outcome. Two researchers, each with relevant professional backgrounds, independently evaluated study quality and extracted data from the selected studies. Meta-analysis was carried out using R Studio version 4.3.1.</p><p><strong>Results: </strong>In total, 30 studies were included, with 13 selected for meta-analysis. The meta-analysis results revealed that genetically predicted high levels of some metabolites may be associated with a reduced risk of AD. (HDL-C: OR = 0.90, 95% CI 0.83-0.97, p = 0.004; Testosterone: OR = 0.93, 95% CI 0.90-0.97, p = 0.001; Male hormones _{exclude testosterone}: OR = 0.85, 95% CI 0.75-0.96, p = 0.007; Glutamine: OR = 0.85, 95% CI 0.81-0.89, p < 0.001) Meanwhile, genetically predicted high LDL-C levels are associated with an increased risk of AD. (LDL-C: OR = 1.52, 95% CI 1.15-2.00, p = 0.003). There is not enough evidence to prove that there is a genetic causal relationship between diabetes and AD. (OR = 1.02, 95% CI 1.00-1.03, p = 0.12).</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 3","pages":"53"},"PeriodicalIF":3.5,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12011960/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic and morphometric analysis of allometric and total liver growth in Post-Hatch chickens.","authors":"Heidi Van Every, Carl J Schmidt","doi":"10.1007/s11306-025-02250-2","DOIUrl":"https://doi.org/10.1007/s11306-025-02250-2","url":null,"abstract":"<p><strong>Introduction: </strong>This study examines metabolic and morphometric changes in chicken liver metabolism during the post-hatch growth period (days 4-20). During this period, liver metabolism transitions from using yolk-derived lipids to feed derived carbohydrates and proteins. The period also encompasses distinct growth phases with implications for metabolic impacts on total and allometric (proportional) growth.</p><p><strong>Objectives: </strong>Identify shifts in metabolites and pathways that occur during the change in nutrients and relate these to patterns of total and allometric liver growth.</p><p><strong>Methods: </strong>Liver samples were collected every other day between days 4-20 and analyzed using metabolomic and morphometric approaches to relate metabolic changes to growth. Principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to identify trends in the data. Cross-validation ANOVA, and network analyses were applied to evaluate metabolic changes across the time periods.</p><p><strong>Results: </strong>Three liver growth periods were defined. Period A (days 4-8) exploited stored nutrients to support rapid growth. Period B (days 10-14) was transitional as stored nutrients were depleted and feed became the major metabolic driver. By period C (days 16-20) the liver is fully dependent on feed. Positive allometric growth occurs predominantly during period A while the organ continues to grow throughout the entire time.</p><p><strong>Conclusions: </strong>Metabolic pathways exhibit distinct networks as nutrient resources change over the early post-hatch period. These findings provide a framework for understanding how nutrient-driven metabolism influences liver scaling and functional maturation.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 3","pages":"52"},"PeriodicalIF":3.5,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11993442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144032873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 2025 Metabolomics publication awards.","authors":"Royston Goodacre","doi":"10.1007/s11306-025-02251-1","DOIUrl":"https://doi.org/10.1007/s11306-025-02251-1","url":null,"abstract":"","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 3","pages":"51"},"PeriodicalIF":3.5,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SARS-CoV2 variants differentially impact on the plasma metabolome.","authors":"Tina Kramaric, Onn Shaun Thein, Dhruv Parekh, Aaron Scott, Andrine Vangberg, Manfred Beckmann, Helen Phillips, David Thickett, Luis A J Mur","doi":"10.1007/s11306-025-02238-y","DOIUrl":"10.1007/s11306-025-02238-y","url":null,"abstract":"<p><strong>Introduction: </strong>Infection with severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) leads to COVID19 disease and caused a worldwide pandemic in 2019. Since the first wave of infections, there has been significant antigenic shifts, leading to the emergence of new variants. Today, infections have shifted away from the severe, fatal infection seen in 2019.</p><p><strong>Objective: </strong>This study aimed to assess how the plasma metabolomes from patients varied with infection with different strains and could reflect disease severity.</p><p><strong>Methods: </strong>Patients with COVID19 not requiring intensive care were recruited between January 2021 and May 2022 from the Queen Elizabeth Hospital Birmingham; 33 patients with alpha, 13 delta and 14 omicron variants. These were compared to 26 age matched contemporaneously recruited controls. Plasma samples were extracted into chloroform/methanol/water (1:2.5/1 v/v) and assessed by flow injection electrospray mass spectrometry (FIE-MS) using an Exactive Orbitrap mass spectrometer. Derived data were assessed using the R based MetaboAnalyst platform.</p><p><strong>Results: </strong>Plasma metabolomes from COVID19 patients were clearly different from controls. Metabolite variation could be related to infection with different SARS-CoV2 variants. Variant showed different levels of some phospholipids, ganglioside GD1a and a dihydroxyvitamin D3 derivative. Correlations of the plasma metabolomes indicated negative correlations between selected phospholipids and the levels of C-reactive protein, creatinine, neutrophil and D-dimer.</p><p><strong>Conclusion: </strong>The plasma metabolomes of COVID19 patients show changes, particularly in phospholipids, which could reflect disease severity and SARS-CoV2 variant infection.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 2","pages":"50"},"PeriodicalIF":3.5,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Metabolomic heterogeneity of ageing with ethnic diversity: a step closer to healthy ageing.","authors":"Drupad Trivedi, Katherine A Hollywood, Yun Xu, Fredrick C W Wu, Drupad K Trivedi, Royston Goodacre","doi":"10.1007/s11306-025-02247-x","DOIUrl":"10.1007/s11306-025-02247-x","url":null,"abstract":"","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 2","pages":"48"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11961457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pilot study on hemodynamically stable isolated chest trauma patients reveals dysregulation of oxidative metabolism.","authors":"Arun Kumar Malaisamy, Ramesh Vaidyanathan, Anand Kumar, Narendra Choudhary, Pratyusha Priyadarshini, Dinesh Kumar Bagaria, Arulselvi Subramanian, Kapil Dev Soni, Abhinav Kumar, Neel Sarovar Bhavesh","doi":"10.1007/s11306-025-02241-3","DOIUrl":"10.1007/s11306-025-02241-3","url":null,"abstract":"<p><strong>Background: </strong>Metabolomic dysregulation precedes clinical deterioration following injury. However, despite receiving comparable treatment, patients with similar injury severity often follow different clinical trajectories and outcomes.</p><p><strong>Methods: </strong>This prospective cohort study at a level 1 trauma centre screened 4541 acutely injured patients with chest trauma between September 2019 and February 2023. Fifty hemodynamically stable patients with isolated chest trauma were recruited for the final analysis. Urine samples were collected on the injury days 1, 3, and 7. For healthy subjects, the urine sample was collected once. NMR-based metabolomics was performed.</p><p><strong>Results: </strong>The study found that the majority of injured patients were young (median age of 40 years), with road traffic injuries being the most common. The median time to presentation of the patient to the ED was 3.08 h, and 92% of patients had multiple rib fractures, pulmonary contusion (60%), and pleural involvement (88%). No patient died. The study found that twenty metabolites were dysregulated (p-value < 0.001). Twelve metabolites were upregulated, while the other eight showed downregulation. However, only five metabolites showed temporal association. 4-HPA, phenylalanine, aconitate, and carnitine represent a high potential for use as a biomarker in patients with isolated blunt trauma chest patients who remain hemodynamically stable. These differentially regulated metabolites were involved in Glyoxylate and dicarboxylate metabolism pathways, glycine, serine, and threonine metabolism, and the Citrate cycle (TCA cycle).</p><p><strong>Conclusions and relevance: </strong>Metabolomics can accurately characterize metabolism in isolated blunt chest trauma patients, revealing perturbed pathways of traits such as oxidative stress and amino acid metabolisms. These metabolites could serve as biomarkers to detect systemic changes following chest injuries early. Metabolic profiling following an injury can aid in detecting systemic changes early and identifying novel biomarkers, enabling targeted interventions to improve patient outcomes.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 2","pages":"49"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}