Metabolomics最新文献

{"title":"SARS-CoV2 variants differentially impact on the plasma metabolome.","authors":"Tina Kramaric, Onn Shaun Thein, Dhruv Parekh, Aaron Scott, Andrine Vangberg, Manfred Beckmann, Helen Phillips, David Thickett, Luis A J Mur","doi":"10.1007/s11306-025-02238-y","DOIUrl":"10.1007/s11306-025-02238-y","url":null,"abstract":"<p><strong>Introduction: </strong>Infection with severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) leads to COVID19 disease and caused a worldwide pandemic in 2019. Since the first wave of infections, there has been significant antigenic shifts, leading to the emergence of new variants. Today, infections have shifted away from the severe, fatal infection seen in 2019.</p><p><strong>Objective: </strong>This study aimed to assess how the plasma metabolomes from patients varied with infection with different strains and could reflect disease severity.</p><p><strong>Methods: </strong>Patients with COVID19 not requiring intensive care were recruited between January 2021 and May 2022 from the Queen Elizabeth Hospital Birmingham; 33 patients with alpha, 13 delta and 14 omicron variants. These were compared to 26 age matched contemporaneously recruited controls. Plasma samples were extracted into chloroform/methanol/water (1:2.5/1 v/v) and assessed by flow injection electrospray mass spectrometry (FIE-MS) using an Exactive Orbitrap mass spectrometer. Derived data were assessed using the R based MetaboAnalyst platform.</p><p><strong>Results: </strong>Plasma metabolomes from COVID19 patients were clearly different from controls. Metabolite variation could be related to infection with different SARS-CoV2 variants. Variant showed different levels of some phospholipids, ganglioside GD1a and a dihydroxyvitamin D3 derivative. Correlations of the plasma metabolomes indicated negative correlations between selected phospholipids and the levels of C-reactive protein, creatinine, neutrophil and D-dimer.</p><p><strong>Conclusion: </strong>The plasma metabolomes of COVID19 patients show changes, particularly in phospholipids, which could reflect disease severity and SARS-CoV2 variant infection.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 2","pages":"50"},"PeriodicalIF":3.5,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11972186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143788675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Metabolomic heterogeneity of ageing with ethnic diversity: a step closer to healthy ageing.","authors":"Drupad Trivedi, Katherine A Hollywood, Yun Xu, Fredrick C W Wu, Drupad K Trivedi, Royston Goodacre","doi":"10.1007/s11306-025-02247-x","DOIUrl":"10.1007/s11306-025-02247-x","url":null,"abstract":"","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 2","pages":"48"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11961457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pilot study on hemodynamically stable isolated chest trauma patients reveals dysregulation of oxidative metabolism.","authors":"Arun Kumar Malaisamy, Ramesh Vaidyanathan, Anand Kumar, Narendra Choudhary, Pratyusha Priyadarshini, Dinesh Kumar Bagaria, Arulselvi Subramanian, Kapil Dev Soni, Abhinav Kumar, Neel Sarovar Bhavesh","doi":"10.1007/s11306-025-02241-3","DOIUrl":"https://doi.org/10.1007/s11306-025-02241-3","url":null,"abstract":"<p><strong>Background: </strong>Metabolomic dysregulation precedes clinical deterioration following injury. However, despite receiving comparable treatment, patients with similar injury severity often follow different clinical trajectories and outcomes.</p><p><strong>Methods: </strong>This prospective cohort study at a level 1 trauma centre screened 4541 acutely injured patients with chest trauma between September 2019 and February 2023. Fifty hemodynamically stable patients with isolated chest trauma were recruited for the final analysis. Urine samples were collected on the injury days 1, 3, and 7. For healthy subjects, the urine sample was collected once. NMR-based metabolomics was performed.</p><p><strong>Results: </strong>The study found that the majority of injured patients were young (median age of 40 years), with road traffic injuries being the most common. The median time to presentation of the patient to the ED was 3.08 h, and 92% of patients had multiple rib fractures, pulmonary contusion (60%), and pleural involvement (88%). No patient died. The study found that twenty metabolites were dysregulated (p-value < 0.001). Twelve metabolites were upregulated, while the other eight showed downregulation. However, only five metabolites showed temporal association. 4-HPA, phenylalanine, aconitate, and carnitine represent a high potential for use as a biomarker in patients with isolated blunt trauma chest patients who remain hemodynamically stable. These differentially regulated metabolites were involved in Glyoxylate and dicarboxylate metabolism pathways, glycine, serine, and threonine metabolism, and the Citrate cycle (TCA cycle).</p><p><strong>Conclusions and relevance: </strong>Metabolomics can accurately characterize metabolism in isolated blunt chest trauma patients, revealing perturbed pathways of traits such as oxidative stress and amino acid metabolisms. These metabolites could serve as biomarkers to detect systemic changes following chest injuries early. Metabolic profiling following an injury can aid in detecting systemic changes early and identifying novel biomarkers, enabling targeted interventions to improve patient outcomes.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 2","pages":"49"},"PeriodicalIF":3.5,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress and perspectives of metabolic biomarkers in blood sample for diabetic microvascular complications.","authors":"Li Yan, Xu Wang, Yujie Xiang, Shuyi Ru, Cheng Fang, Xiuhong Wu","doi":"10.1007/s11306-025-02245-z","DOIUrl":"https://doi.org/10.1007/s11306-025-02245-z","url":null,"abstract":"<p><strong>Background: </strong>Diabetes mellitus refers to a group of metabolic diseases characterized by chronic hyperglycemia due to multiple etiological factors. As the disease progresses, patients gradually develop microvascular complications, including diabetic nephropathy, diabetic retinopathy, and diabetic neuropathy. However, current clinical methods for detecting these microvascular complications are limitations, thus primary prevention and early diagnosis are of great importance.</p><p><strong>Aim of review: </strong>This review summarizes the known blood biomarkers of diabetic microvascular complications, classified according to type of structure, including amino acid metabolism, lipid metabolism, carnitine metabolism, organic acid metabolism, etc., which can be used for the simultaneous typing of diabetes mellitus based on microvascular complications, and to search for the trend of changes to lay the foundation for early diagnosis and understanding of the pathogenesis of diabetic microvascular complications, including oxidative stress, and mitochondrial dysfunction. Searches for the trend of changes to lay the foundation for early diagnosis and understanding of the pathogenesis of diabetic microvascular complications, including oxidative stress, mitochondrial dysfunction.</p><p><strong>Key scientific concepts of review: </strong>Due to the limitations of diagnostic criteria for diabetic microvascular complications, some patients already have the disease for which they are being tested. Metabolomics reflects the physiological state of an organism by analyzing the small molecules metabolites present in a biological tissue that are related to clinical phenotypes, providing a snapshot of the physiological and pathophysiological metabolic processes occurring within that organism at any given time, thus opening the door for the development of diagnostic biomarkers and precise treatment. In clinical metabolomics, blood is considered a specialized type of connective tissue, which allows it to transport substances throughout the body, connecting different systems together. Also, blood components are probably the most frequently used matrix in metabolomics studies. Therefore, metabolomics is used to analyze blood biomarkers that reflect the course of diabetes and explore the pathways involved in the pathophysiology of the three most common diabetic microvascular complications. Finally, in this review, we discuss the current limitations of metabolomic analysis, and the integrative multi-omics data, including genomics, transcriptomics, and proteomics, required for developing specific biomarkers for diabetic microvascular complications.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 2","pages":"47"},"PeriodicalIF":3.5,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss-of-functional mutation in ANGUSTIFOLIA3 causes leucine hypersensitivity and hypoxia response during Arabidopsis thaliana seedling growth.","authors":"Kensuke Kawade, Mamoru Nozaki, Gorou Horiguchi, Tomoko Mori, Katsushi Yamaguchi, Mami Okamoto, Hiromitsu Tabeta, Shuji Shigenobu, Masami Yokota Hirai, Hirokazu Tsukaya","doi":"10.1007/s11306-025-02249-9","DOIUrl":"10.1007/s11306-025-02249-9","url":null,"abstract":"<p><strong>Introduction: </strong>The ANGUSTIFOLIA3 (AN3) gene encodes a transcriptional co-activator for cell proliferation in Arabidopsis thaliana leaves. We previously showed that Physcomitrium patens AN3 orthologs promote gametophore shoot formation through arginine metabolism.</p><p><strong>Objectives: </strong>We analyzed the role of AN3 in Arabidopsis thaliana to understand how seedling growth is regulated by metabolic and physiological modulations.</p><p><strong>Methods: </strong>We first explored amino acids that affect the seedling growth of an3 mutants. Transcriptome and metabolome analyses were conducted to elucidate the metabolic and physiological roles of AN3 during seedling growth. Lastly, we examined the distribution of reactive oxygen species to corroborate our omics-based findings.</p><p><strong>Results: </strong>Our results indicated that an3 mutants were unable to establish seedlings when grown with leucine, but not arginine. Multi-omics analyses suggested that an3 mutants exhibit a hypoxia-like response. Abnormal oxidative status was confirmed by detecting an altered distribution of reactive oxygen species in the roots of an3 mutants.</p><p><strong>Conclusion: </strong>AN3 helps maintain the leucine metabolism and oxidative balance during seedling growth in Arabidopsis thaliana. Future research is necessary to explore the interaction between these processes.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 2","pages":"46"},"PeriodicalIF":3.5,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11958482/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the relationship between serum 25-hydroxyvitamin D levels and intestinal fungal communities and their metabolites in postmenopausal Chinese women.","authors":"Han Zhang, Jinhua Gong, Kunpeng Xu, Zixian Dang, Zhen Shang, Guanhong Chen, Haotian Feng, Yuying Zhang, Yingze Zhang, Tengbo Yu, Jianquan He, Wenxin Hong, Yongtao Zhang","doi":"10.1007/s11306-025-02244-0","DOIUrl":"10.1007/s11306-025-02244-0","url":null,"abstract":"<p><strong>Background: </strong>Research gaps persist in understanding the interactions between serum 25 (OH)D levels, intestinal fungi, and their metabolites in postmenopausal women.</p><p><strong>Methods: </strong>This study, approved by the Ethics Committee of Zhongshan Hospital, Xiamen University, recruited postmenopausal women from Xiamen. Clinical assessments included Body Mass Index (BMI) calculations and blood tests for various bone-related markers using Roche's electrochemiluminescence system. Bone density was measured via dual-energy X-ray absorptiometry. Fecal DNA was extracted for Internal Transcribed Spacer (ITS) sequencing with a two-stage PCR process and analyzed using high-throughput Illumina sequencing. Metabolites were extracted from fecal samples and analyzed by ultra-high-performance liquid chromatography combined with mass spectrometry. Statistical analyses and data visualization were performed using R, focusing on fungal community structure and correlations with metabolites.</p><p><strong>Results: </strong>The study analyzed 81 postmenopausal women, categorized into vitamin D deficient (VDD), insufficient (VDI), and sufficient (VDS) groups based on serum 25 (OH)D levels. Other health markers, including age and BMI, were consistent across groups. Notably, Linear discriminant analysis identified distinct fungal communities across VDD, VDI, and VDS groups. In the VDD group, notable fungi included Hanseniaspora occidentalis and Pichia. The VDI group showed enrichment of Candida, while the VDS group had higher abundances Such as Phanerochaete, and Nectriaceae. Alpha diversity metrics, such as the Chao1 index, differed significantly among the groups (p < 0.05). Correlation analysis (Spearman) revealed that fungi like Trichosporon and Penicillium positively associated with 25 (OH)D3, whereas fungi such as Cystofilobasidium were negatively correlated with bone mineral density (BMD). Metabolites like Glutaric acid positively correlated with 25 (OH)D3, while L-Citrulline and Deoxycholic acid were negatively correlated. Additionally, Argininosuccinic acid correlated positively with BMD, whereas Acamprosate and p-Hydroxyphenylacetic acid were negatively associated.</p><p><strong>Conclusion: </strong>In postmenopausal women, fungal community composition varies significantly with vitamin D status, potentially correlating with serum 25 (OH)D levels and BMD, indicating that specific fungal species may be relevant for therapeutic strategies with osteoporosis and offering insights into the broader bone health effects of vitamin D.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 2","pages":"45"},"PeriodicalIF":3.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of metabolites in urine in post-kidney transplant patients: insights into allograft function and creatinine clearance.","authors":"Eva Baranovicova, Matej Vnucak, Karol Granak, Patricia Kleinova, Erika Halasova, Ivana Dedinska","doi":"10.1007/s11306-025-02246-y","DOIUrl":"10.1007/s11306-025-02246-y","url":null,"abstract":"<p><strong>Introduction: </strong>The suboptimal function of transplanted kidney can lead to imbalances in processes controlled by the kidneys, necessitating long-term monitoring of the graft's function and viability. Given the kidneys' high metabolic activity, a metabolomics approach is well-suited for tracking changes in post-transplant patients and holds significant potential for monitoring graft function.</p><p><strong>Objectives: </strong>Examination of the response of urinary creatinine levels to (i) serum creatinine levels and (ii) allograft function during periods of impaired kidney function in post-transplant patients.</p><p><strong>Methods: </strong>We analyzed morning and 24-h urine samples from 55 patients who underwent primary kidney transplantation and were uniformly treated with immunosuppressants, with an average follow-up of 50 months post-surgery. We assessed the relative levels of urinary metabolites detectable by NMR spectroscopy and investigated correlations between these metabolite levels and renal function.</p><p><strong>Results: </strong>We observed rather unexpected independence of urinary creatinine levels on levels of serum creatinine as well as on allograft function expressed by eGFR (estimated glomerular filtration rate). This observation allowed a very good agreement of outcomes from raw and creatinine-normalized data, consistent for both morning urine samples and 24-h urine collections. The urinary levels of citrate and acetone were detected to be sensitive to allograft function, and the urinary levels of metabolites in combination showed promising prediction for kidney function, on the level of p-value: for 24 h pooled urine: 4.6 × 10^-12 and morning urine: 5.36 × 10^-9. We discussed the data also in the light of metabolic changes in blood plasma.</p><p><strong>Conclusion: </strong>We support the opinion of critical assessment of renal creatinine clearance when judging the filtration function of the allograft. As the next, urinary metabolomics can serve as an easily available supplement to prediction for allograft function in patients after kidney transplantation.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 2","pages":"44"},"PeriodicalIF":3.5,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11950123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143720223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin B6 deficiency produces metabolic alterations in Drosophila.","authors":"Giulia Tesoriere, Eleonora Pilesi, Michele De Rosa, Ottavia Giampaoli, Adriano Patriarca, Mariangela Spagnoli, Federica Chiocciolini, Angela Tramonti, Roberto Contestabile, Fabio Sciubba, Fiammetta Vernì","doi":"10.1007/s11306-025-02236-0","DOIUrl":"10.1007/s11306-025-02236-0","url":null,"abstract":"<p><strong>Introduction: </strong>Pyridoxal 5'-phosphate (PLP), the biologically active form of vitamin B6 is involved in 4% of cellular enzymatic activities and its deficiency is responsible for or contributes to several human diseases. The study of underlying mechanisms is still in its infancy and requires suitable model organisms. In Drosophila the deficiency of vitamin B6 produces chromosome aberrations and hallmarks of human diseases including diabetes and cancer. However, the effects of vitamin B6 deficiency have never been examined at a metabolic level.</p><p><strong>Objectives: </strong>This study evaluates the metabolic changes in vitamin B6 deficient Drosophila larvae with the aim of validating flies as a suitable model for diseases associated to vitamin B6 deficiency.</p><p><strong>Methods: </strong>To induce vitamin B6 deficiency we fed Drosophila wild type larvae with 4-deoxypyridoxine (4DP), a PLP antagonist. By HPLC analysis we verified that the 4DP treatment was effective in inducing vitamin B6 deficiency. Using an NMR-based metabolomic approach we compared the metabolites in larval extracts from untreated and 4DP-fed larvae.</p><p><strong>Results: </strong>The NMR spectra analysis identified quantitative differences for sixteen metabolites out of forty, including branched chain and aromatic amino acids, glucose, and lipids, thus revealing interesting possible associations with the phenotypes showed by vitamin B6 deficient flies.</p><p><strong>Conclusions: </strong>Our results validate Drosophila as a suitable model to study in depth the molecular mechanisms underlying human diseases associated with vitamin B6 deficiency and confirmed that 4DP treatment is effective in inducing vitamin B6 deficiency.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 2","pages":"42"},"PeriodicalIF":3.5,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11930875/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic profiling in experimental guinea pig models of bacterial and allergic inflammation.","authors":"J Hanusrichterova, E Baranovicova, R Barosova, M Kolomaznik, P Mikolka, P Kosutova, D Mokra, J Mokry, A Calkovska","doi":"10.1007/s11306-025-02239-x","DOIUrl":"10.1007/s11306-025-02239-x","url":null,"abstract":"<p><strong>Introduction: </strong>Based on distinct triggers, bacterial and allergen-induced inflammatory reactions have different pathophysiology. Metabolomic analysis is high-throughput technique that can provide potential biomarkers to distinguish between these responses.</p><p><strong>Objectives: </strong>In order to find out the metabolic profiles of two types of inflammation, metabolites were analysed in blood plasma and bronchoalveolar lavage fluid (BALF) of guinea pigs subjected to bacterial lipopolysaccharide (LPS) or allergen ovalbumin (OVA).</p><p><strong>Methods: </strong>Hydrogen-1 nuclear magnetic resonance (¹H NMR) spectroscopy for metabolite analysis was performed in samples of blood plasma and BALF of guinea pigs.</p><p><strong>Results: </strong>Random forest algorithm built on combination of levels of circulating and BALF metabolites resulted in almost ideal discrimination between acute allergic and bacterial inflammation. The differences between inflammation triggered by LPS and OVA were manifested in shift in energy metabolism, metabolism of branched-chain amino acids (BCAAs)/branched-chain keto acids (BCKAs) with alterations in alanine and glutamine, which are linked with both, ammonia homeostasis as well as gluconeogenesis.</p><p><strong>Conclusion: </strong>Distinct molecule nutrients are to be utilized during acute bacterial and allergic inflammatory response.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 2","pages":"43"},"PeriodicalIF":3.5,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11930882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corroborated evidence on change of metabolome after ischemic stroke due to large vessel occlusion.","authors":"Evgeny V Sidorov, Kyle Smith, Chao Xu, Madhusmita Route, Dharambir K Sanghera","doi":"10.1007/s11306-025-02235-1","DOIUrl":"https://doi.org/10.1007/s11306-025-02235-1","url":null,"abstract":"<p><strong>Introduction: </strong>Metabolomic studies which search for acute ischemic stroke (AIS) biomarkers commonly have contradictory findings. Robust methodology is required to understand true metabolome changes after AIS.</p><p><strong>Methods: </strong>To improve validity, we obtained corroborative evidence on change of serum metabolome after AIS by: (1) focusing on patients with large vessel occlusion (LVO), (2) combining cross-sectional and longitudinal study designs, and (3) performing analysis using different metabolome platforms: Nuclear Magnetic Resonance (NMR) and Liquid Chromatography-Mass Spectrometry (LC-MS). In the cross-sectional part we compared serum metabolome of 84 AIS patients and 82 controls using NMR at 48-72 h, while in the longitudinal part we prospectively analyzed serum metabolome using LC-MS on 15 AIS patients at < 24 h, 48-72 h, 5-7 days, 80-120 days. We hypothesized that serum metabolites elevated in cross-sectional part would show rising trajectory in longitudinal part, and vice versa.</p><p><strong>Results: </strong>We found that glycerol, phosphatidylethanolamine (PE), ceramide, phenylalanine and their derivatives had consistent increases, while other key metabolites including histidine, tyrosine, valine, glutamine, phosphatidylcholine (PC), sphingomyelin, fatty acids (FA) had consistent decreases after AIS.</p><p><strong>Conclusion: </strong>We identified corroborated changes in metabolome after AIS across different technologies and study designs. These changes correspond to loss of nerve cell membrane integrity and activation of alternative metabolic pathways in the setting of blood brain barrier (BBB) disruption. If proven on a larger sample, our findings may improve prediction of mortality, and functional outcomes after AIS.</p>","PeriodicalId":18506,"journal":{"name":"Metabolomics","volume":"21 2","pages":"41"},"PeriodicalIF":3.5,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}