MEDICAL SCIENCES JOURNAL最新文献_第10页

The effect of 4 weeks high-intensity interval training (HIIT) on the content of downstream and upstream mTORC1 pathways gastrocnemius muscle of type 2 diabetic rats 4周高强度间歇训练(HIIT)对2型糖尿病大鼠腓肠肌上下游mTORC1通路含量的影响

MEDICAL SCIENCES JOURNAL Pub Date : 2020-06-10 DOI: 10.29252/iau.30.2.120

Golnaz Faezi, M. S. Moghadam, S. - Shadmehri, M. Fathalipour

{"title":"The effect of 4 weeks high-intensity interval training (HIIT) on the content of downstream and upstream mTORC1 pathways gastrocnemius muscle of type 2 diabetic rats","authors":"Golnaz Faezi, M. S. Moghadam, S. - Shadmehri, M. Fathalipour","doi":"10.29252/iau.30.2.120","DOIUrl":"https://doi.org/10.29252/iau.30.2.120","url":null,"abstract":"Background: The pathway of mTORC1 is one of the most important pathway in protein synthesis, and type 2 diabetes and insulin resistance can lead to inhibit this pathway. The aim of this study was to investigate the effect of 4 weeks high-intensity interval training (HIIT) on the content of downstream and upstream mTORC1 pathways in gastrocnemius muscle in type 2 diabetic rats. Materials and methods: In this experimental study, 16 Sprague-Dawley male rats (with mean weight of 250 ± 20 gr) were selected and after induction of diabetes by streptozotocin and nicotinamide, the rats were randomly assigned into two groups, including diabetic HIIT training and diabetic control. The experimental group performed 4 days a week the exercise training for 4 weeks, while the control group did not have any training program. Independent T-test was used to analyze the data. Results: A significant change was not observed in the total content of AKT1 proteins (p<0.31), P70S6K1 (p<0.69) and 4EBP1 (p<0.84) in the HIIT training group compared to the control group, but the total protein content of mTOR (p<0.02) and the form of phosphorylation of AKT1 (p<0.03), mTOR (p<0.03), P70S6K1 (p<0.02) and 4EBP1 (p<0.009) proteins showed significant increase in training group compared to the control group. Conclusion: The HIIT training can probably activate the pathway of AKT1/mTOR/P70S6K1 and AKT1/mTOR/4EBP1 in the mTORC1 pathway; therefore, the HIIT training can lead to protein synthesis or muscle hypertrophy through this pathway in the gastrocnemius muscle of the trained rats","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78323539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Theoretical study of interaction between aspirine drug and Al-soped graphene nanostructure toward designing of suitable nanocarrier for drug delivery 阿斯匹林药物与Al-soped石墨烯纳米结构相互作用的理论研究有助于设计合适的药物递送纳米载体

MEDICAL SCIENCES JOURNAL Pub Date : 2020-06-01 DOI: 10.29252/iau.30.2.141

سارا فرشاد, مسعود درویش گنجی

{"title":"Theoretical study of interaction between aspirine drug and Al-soped graphene nanostructure toward designing of suitable nanocarrier for drug delivery","authors":"سارا فرشاد, مسعود درویش گنجی","doi":"10.29252/iau.30.2.141","DOIUrl":"https://doi.org/10.29252/iau.30.2.141","url":null,"abstract":"Background: In recent years, the unique physical and chemical properties of carbon nanostructures has led to many advancements in various fields, including chemistry and pharmaceuticals. Graphene is one of the carbon nanostructures which have attracted significant attention from researchers in adsorption and release of various drugs. Due to the high surface area of graphene, it can be used as a biological carrier in drug delivery. In this study, the interaction of aspirin with a graphene sheet doped with aluminum (graphenealuminum) and possibility of stable complex formation between them were investigated using the theoretical study. Materials and methods: The performance of carbon nanostructures for adsorption of aspirin on graphenealuminum was evaluated using quantum computation. The calculations were performed using density functional theory modified with dispersion forces (DFT-D) and basic functions by using of ORCA software. Results: Adsorption energy and electronic structure of aspirin /graphene-aluminum system were calculated. The measured adsorption energy and bond distance were −53.08 (kcal/mol) and 1.888 Å, respectively. The distribution of electron charge also indicated the continuity of electron clouds between drugs and nanostructure. Conclusion: The results showed that a strong bond formed between aspirin and graphene-aluminum and the complex formed in the aqueous medium was thermodynamically stable. Regarding the possibility of stable complex formation, graphene-aluminum was expected to be suitable nanocarier for delivery of aspirin to target cells.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"47 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79464631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The effect of positive thinking training on happiness and resilience of nurses in intensive care units: a randomized clinical trial 积极思维训练对重症监护室护士幸福感和心理弹性的影响:随机临床试验

MEDICAL SCIENCES JOURNAL Pub Date : 2020-06-01 DOI: 10.29252/iau.30.2.193

پانته آ کیانی, سید محمد کاظم نائینی, محبوبه صفوی

{"title":"The effect of positive thinking training on happiness and resilience of nurses in intensive care units: a randomized clinical trial","authors":"پانته آ کیانی, سید محمد کاظم نائینی, محبوبه صفوی","doi":"10.29252/iau.30.2.193","DOIUrl":"https://doi.org/10.29252/iau.30.2.193","url":null,"abstract":"Background: Nursing in intensive care units is associated with various stressors that can affect the psychological state. In this study, the effect of positive thinking training on the happiness and resiliency of nurses in intensive care units was investigated. Materials and methods: A total of 60 nurses from Shohadaye Tajrish and Shahid Taleghani hospitals of Tehran City were randomly divided into two groups. The intervention group received teaching positive thinking skills for 8 sessions (each session was 1.5 hours). Data were collected using a three-part questionnaire on demographic data, Oxford Happiness Index (OHI), and Connor-Davidson Resilience Scale (CD-RISC). Data were analyzed using SPSS version 23 software. Results: Demographic variables had no significant differences between two groups. There was no significant difference in the mean of happiness and resiliency in the pre-test stage between two groups. The results of covariance analysis indicated the effectiveness of intervention in increasing happiness and resiliency, so that 55% of changes in variance of happiness and 43% of changes in variance of resiliency were due to independent variable. Conclusion: Training of positive thinking skills improved nurses' happiness and resilience. These interventions are an uncomplicated and effective method that can be used extensively in nurses to improve psychological components.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80156525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The effect of agonist and antagonist of Nociceptine/Orphanin FQ receptor on seizure and cognitive dysfunction in experimental model of temporal lobe epilepsy in male rat 诺西西汀/孤啡肽FQ受体激动剂和拮抗剂对雄性大鼠颞叶癫痫实验模型癫痫发作和认知功能障碍的影响

MEDICAL SCIENCES JOURNAL Pub Date : 2020-06-01 DOI: 10.29252/iau.30.2.107

N. Jamali-Raeufy, M. Zeinivand, Mina Goudarzi, Sobhan Haghani

{"title":"The effect of agonist and antagonist of Nociceptine/Orphanin FQ receptor on seizure and cognitive dysfunction in experimental model of temporal lobe epilepsy in male rat","authors":"N. Jamali-Raeufy, M. Zeinivand, Mina Goudarzi, Sobhan Haghani","doi":"10.29252/iau.30.2.107","DOIUrl":"https://doi.org/10.29252/iau.30.2.107","url":null,"abstract":"Background: Temporal lobe epilepsy is a chronic neurological disorder characterized by spontaneous seizures, learning and memory deficiency, loss of neurons, mossy fiber sprouting and tissue apoptosis. This study was to investigate the effect of NOP receptor agonist (MCOPPB) and antagonist (SB612111) on seizure and cognitive dysfunction and histological studies in experimental model of temporal lobe epilepsy in male rat. Materials and methods: in this study, 50 male rats were divided into six groups, including sham, epileptic, valperoic treated epileptic, NOP receptor agonist and antagonist treated epileptic. Finally, seizure behavior, short-term (Y-maze) and long-term (shuttle box) memory, GFAP value and also histologic finding (Nissel, Tim and Apoptosis staining) were evaluated. Results: Kinic acid induced seizures associated with significant seizure behavior, impairment of learning and memory and tissue damage. Pretreated epileptic rats with NOP receptor agonist decreased seizure attacks, but did not improve memory. Administration of NOP antagonist was not effective on the seizure behavior, but contribute to improve the memory and learning abilities following treatment. Also, administration of NOP agonist and antagonist increased neuron count, reduced increased sprouting of mossy fibers, cell death and the activity of astrocytes in the hippocampus. Conclusion: pre-treatment of epileptic rets with NOP receptor agonist and antagonist reduced seizures attacks and improved short-term spatial memory and tissue damage in rats.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78621989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Paraneoplastic hypocalcemia in a patient whit gastric cancer accompanied by osteoblastic metastasis 胃癌伴成骨细胞转移的副肿瘤性低钙症1例

MEDICAL SCIENCES JOURNAL Pub Date : 2020-06-01 DOI: 10.29252/iau.30.2.221

A. Shahrasbi, Kosar Heydarzadeh, Sepideh Fahimi, Nazila Abbasi fard

引用次数: 0

The effects of chlorodiazepoxide on the Bax gene expression in CA1 pyramidal cells of the newborn rat hippocampus 氯二氮环氧化物对新生大鼠海马CA1锥体细胞Bax基因表达的影响

MEDICAL SCIENCES JOURNAL Pub Date : 2020-06-01 DOI: 10.29252/iau.30.2.134

A. Dinarvand, M. Hashemi, Rasool Dinarvand, Shabnam Movaseghi, Mojtaba Jafarinia

{"title":"The effects of chlorodiazepoxide on the Bax gene expression in CA1 pyramidal cells of the newborn rat hippocampus","authors":"A. Dinarvand, M. Hashemi, Rasool Dinarvand, Shabnam Movaseghi, Mojtaba Jafarinia","doi":"10.29252/iau.30.2.134","DOIUrl":"https://doi.org/10.29252/iau.30.2.134","url":null,"abstract":"Background : Most drugs used by pregnant women can pass through the placenta and expose embryos and developing embryos to teratogenic effects. Due to the increased cellular damage of Bax gene expression, the effect of cholorodiazepoxide use during pregnancy on Bax gene expression in the hippocampus of neonatal rats was investigated. Materials and methods : After confirmation of pregnancy in Wistar female rats, intraperitoneal injection of chlordiazepoxide was administered at a dose of 10 mg/ kg daily for 21 days in the experimental group. Two weeks after birth, the infant's brain was removed from the skull. After isolating the CA1 region of the neonatal hippocampus, the expression of the Bax proapoptotic gene was examined and compared with the control and carrier group (saline). Results : The level of gene expression was analyzed and propoptotic Bax gene showed significant increase in experimental group compared to control group. Conclusion : The results of this study indicated that the administration of chlorodiazepoxide during pregnancy can cause neuronal damage in the hippocampus of Wistar rats.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83303536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Infantile colic: from basics to treatment 婴儿绞痛:从基础到治疗

MEDICAL SCIENCES JOURNAL Pub Date : 2020-04-01 DOI: 10.29252/iau.30.1.1

H. Masoodi, S. Rahimi, Golvash Tavakolian, S. A. Badi, Shahrbanoo Keshavarz Azizi Raftar, S. Siadat

{"title":"Infantile colic: from basics to treatment","authors":"H. Masoodi, S. Rahimi, Golvash Tavakolian, S. A. Badi, Shahrbanoo Keshavarz Azizi Raftar, S. Siadat","doi":"10.29252/iau.30.1.1","DOIUrl":"https://doi.org/10.29252/iau.30.1.1","url":null,"abstract":"Infantile colic is a common condition among infants in which inability of caregivers to console their infants can lead to shaken baby syndrome, child abuse, depression and etc. Therefore it can lay a great economic burden in healthcare and social welfare systems. Even though it is highly prevalent, little agreement has been reached on the definition, pathogenesis or the optimal management strategy for infantile colic. In the past decade major leaps in understanding infantile colic has been taken by researchers. Although our understanding is greater than before, but novel treatments has yet to be introduced. The current review aims to delineate the Rome IV criteria definition, which is the latest published diagnostic criteria. Moreover, gastrointestinal, neurogenic, microbial and psychosocial factors that might contribute to the pathophysiology of infant colic are explored. Also, clinical assessment of infant with suspected colic is reviewed. Important aspect of the management of infant colic is further highlighted by this review. Management strategies, including dietary, behavioral, pharmacological and alternative interventions are also discussed.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"504 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77455397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of adiponectin level with biochemical variables and insulin resistance in patients with non-alcoholic fatty liver disease in an Iranian population 伊朗人群非酒精性脂肪肝患者脂联素水平与生化变量和胰岛素抵抗的关系

MEDICAL SCIENCES JOURNAL Pub Date : 2019-12-10 DOI: 10.29252/iau.29.4.329

مجتبی رحمانی, معصومه نژادعلی, جواد رستگارمقدم

{"title":"Association of adiponectin level with biochemical variables and insulin resistance in patients with non-alcoholic fatty liver disease in an Iranian population","authors":"مجتبی رحمانی, معصومه نژادعلی, جواد رستگارمقدم","doi":"10.29252/iau.29.4.329","DOIUrl":"https://doi.org/10.29252/iau.29.4.329","url":null,"abstract":"Background: Non-alcoholic fatty liver disease is one of the most important chronic liver disorders worldwide. Adipokines are polypeptide hormones that participate in the pathogenesis of non-alcoholic fatty liver disease. Adiponectin is an adipokine that stimulates fatty acid oxidation in muscle and plays a role in the development of insulin sensitivity. This study aimed to investigate the association between plasma adiponectin and nonalcoholic fatty liver disease. Materials and methods: This case and control study was conducted on 80 non-alcoholic fatty liver disease patients and 80 participants as control group. Plasma levels of adiponectin and insulin were measured by ELISA kit (Mercodia Company, Sweden) and other variables were determined by standard methods. Statistical analysis was performed using SPSS software, version 19. Results: Plasma adiponectin was significantly lower in non-alcoholic fatty liver disease patients than control (p-value<0.001). Whereas, subjects with non-alcoholic fatty liver disease had significantly higher levels of body mass index, serum transaminases, serum triglycerides, systolic blood pressure, serum insulin and insulin resistance compared to control group (p<0.01). An inverse correlation was observed between adiponectin with serum transaminases, triglycerides and insulin resistance (P<0.05), while it did not correlate with lipoproteins. Conclusion: The results indicate that adiponectin levels are reduced in individuals with non-alcoholic fatty liver disease. Adiponectin levels are inversely correlated with liver transaminases and insulin resistance.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77909086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Investigating the prevalence of resistance genes in Listeria monocytogenes isolated from food and clinical samples 调查从食品和临床样本中分离的单核细胞增生李斯特菌耐药基因的流行情况

MEDICAL SCIENCES JOURNAL Pub Date : 2019-12-10 DOI: 10.29252/iau.29.4.322

A. Shivaee, Shahla Shahbazi, A. Gholami, Parham Kianoush Pour, F. Jazi

{"title":"Investigating the prevalence of resistance genes in Listeria monocytogenes isolated from food and clinical samples","authors":"A. Shivaee, Shahla Shahbazi, A. Gholami, Parham Kianoush Pour, F. Jazi","doi":"10.29252/iau.29.4.322","DOIUrl":"https://doi.org/10.29252/iau.29.4.322","url":null,"abstract":"Background: Listeriosis can be fatal for vulnerable groups of society. The disease has been widespread in recent years due to the large consumption of dairy and meat products. There is little information about the susceptibility of antibiotics and the pattern of Listeria monostigenesis gene resistance in Iranian society. Accordingly, the present study was conducted to investigate the antibiotic susceptibility and genetic resistance pattern of Listeria monosteogenesis strains isolated from different clinical and environmental sources. Materials and methods: In this study, 55 isolates were tested for antibiotic susceptibility by disk diffusion in agar and genetic pattern by polymerase chain reaction (PCR). Results: 91% and 83% of the strains were resistant to streptomycin and Trimethoprim/sulfamethoxazole respectively. The result of PCR of antibiotic resistance genes showed that the prevalence of ermA, ermB, strA, tetA, tetS and ermC genes in isolates of Listeria monocytogenes was 50.90% (28/55), 21.81% (12/55), 89.9% (49/55), 0% (0/55), 21.81% (12/55) and 0% (0/55), respectively. Conclusion: Due to the presence of 1/2a and 1/2c serotypes in isolated isolates and the presence of marker virulence genes in these strains, these isolates have potential for biological risks and listeriosis disease. Existence of this genetic pattern and resistance pattern can be partly due to the use of antibiotics during the production of dairy products. Regarding results of this study, the manner and rate of using animal antibiotics can be managed.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"94 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91446398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of charantin on expression of Ngn3 gene in streptozotocin-induced diabetic rats charantin对链脲佐菌素诱导的糖尿病大鼠Ngn3基因表达的影响

MEDICAL SCIENCES JOURNAL Pub Date : 2019-12-10 DOI: 10.29252/iau.29.4.303

Abolfazl Nasirzadeh Vanhari, H. Sazgar, in Biology

{"title":"The effect of charantin on expression of Ngn3 gene in streptozotocin-induced diabetic rats","authors":"Abolfazl Nasirzadeh Vanhari, H. Sazgar, in Biology","doi":"10.29252/iau.29.4.303","DOIUrl":"https://doi.org/10.29252/iau.29.4.303","url":null,"abstract":"Background: Positive effects of medicinal herbs on diabetes have been demonstrated in previous studies. The aim of the present study was to evaluate the effect of charantin on the treatment of diabetes and increase the expression of the Ngn3 gene in diabetes, which is a key gene for transcription of the pancreas. Materials and methods: In this experimental study, 42 adult male Wistar rats were randomly divided into 7 healthy control groups, diabetic control, control group, 150 control, and three groups receiving effective doses of charantin (50-100-200 mg/ Per kilogram). All groups except diabetic control group and control group with streptozotocin chemical injected intraperitoneally. Afterwards, they received a cartilage by gavage for four weeks (three times a week). Results: The lowest expression was observed in the diabetic group. Among those who received different doses of charantine, the highest expression was among group with dose of 100 mg/kg, and the lowest expression in 50/kg group. Conclusion: It can be concluded that increased expression of the Ngn3 gene was not dose-dependent. After the development of diabetes, the patient's severity declined sharply, and after the use of the charantin and metformin drug, this reduction was somewhat offset.","PeriodicalId":18492,"journal":{"name":"MEDICAL SCIENCES JOURNAL","volume":"106 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73545708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0