Methods in molecular biology最新文献_第9页

Zymography for the Detection of Bacterial Proteases. 酶谱法检测细菌蛋白酶。

Methods in molecular biology Pub Date : 2025-01-01 DOI: 10.1007/978-1-0716-4482-9_16

Mary E Marquart

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ELISA for Detection of Total IgG Against YF 17DD. ELISA检测YF - 17DD总IgG。

Methods in molecular biology Pub Date : 2025-01-01 DOI: 10.1007/978-1-0716-4458-4_3

Ana Carolina R A Cajaraville

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An Improved Two-Dimensional HPLC Method for Endogenous 2'-Deoxy-ADPR and 2'-Deoxy-NAD. 内源性2’-脱氧- adpr和2’-脱氧- nad的改进二维高效液相色谱法

Methods in molecular biology Pub Date : 2025-01-01 DOI: 10.1007/978-1-0716-4414-0_6

Feng Gu, Ralf Fliegert, Andreas Bauche, Andreas H Guse

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Yellow Fluorescent Protein Quenching Assay for Analyzing Odorant Receptor Activity. 黄色荧光蛋白猝灭法分析气味受体活性。

Methods in molecular biology Pub Date : 2025-01-01 DOI: 10.1007/978-1-0716-4466-9_11

JiWoo Choi, JaeHyung Koo

引用次数: 0

Dissecting the Heterogeneity of Senescent Cells Through CITE-seq Integration. 通过CITE-seq整合分析衰老细胞的异质性。

Methods in molecular biology Pub Date : 2025-01-01 DOI: 10.1007/978-1-0716-4434-8_7

Kotb Abdelmohsen, Krystyna Mazan-Mamczarz, Dimitrios Tsitsipatis, Martina Rossi, Rachel B Munk, Myriam Gorospe

{"title":"Dissecting the Heterogeneity of Senescent Cells Through CITE-seq Integration.","authors":"Kotb Abdelmohsen, Krystyna Mazan-Mamczarz, Dimitrios Tsitsipatis, Martina Rossi, Rachel B Munk, Myriam Gorospe","doi":"10.1007/978-1-0716-4434-8_7","DOIUrl":"https://doi.org/10.1007/978-1-0716-4434-8_7","url":null,"abstract":"Cellular senescence, a state of persistent growth arrest following cell damage, is associated with aging and age-related diseases. Understanding cell heterogeneity within senescent populations is crucial for developing therapies to mitigate senescence-associated pathologies. The protocol described here outlines an integrated approach to exploit the presence of cell surface proteins on subsets of senescent cells to study their heterogeneity at the single-cell level. After identifying senescence-associated surface proteins by mass spectrometry (MS) and then performing cellular indexing of transcriptomes and epitopes sequencing (CITE-seq) single-cell analysis, we were able to identify unique transcriptomic programs associated with specific surface protein markers expressed in some senescent cells but not in others. We illustrate the utility of this approach by investigating the complex heterogeneity of senescent cell populations. However, this methodology can be applied to other biological scenarios where cells with unique transcriptomic profiles can be studied individually, thanks to the presence of specific cell surface proteins that distinguish them from other cells within the same population.","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":"2908 ","pages":"99-109"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990074","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanoparticles-Assisted Peptide Cancer Vaccine Delivery. 纳米颗粒辅助多肽癌症疫苗递送。

Methods in molecular biology Pub Date : 2025-01-01 DOI: 10.1007/978-1-0716-4542-0_10

Lanqing Luo, Rui Kuai

引用次数: 0

Micro-Focus Reduction Neutralization Tests (mFRNT) for Neutralizing Antibody (Nab) Quantification Against Yellow Fever Virus. 测定黄热病病毒中和抗体（Nab）的微焦点减少中和试验（mFRNT）

Methods in molecular biology Pub Date : 2025-01-01 DOI: 10.1007/978-1-0716-4458-4_17

Adriana de Souza Azevedo, Nathalia Dos Santos Alves

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A Method for H2A.Z/H2B Dimer Exchange Within Nucleosomes In Vitro. A H2A的方法。核小体内Z/H2B二聚体的体外交换。

Methods in molecular biology Pub Date : 2025-01-01 DOI: 10.1007/978-1-0716-4486-7_3

Benoit Guillemette, Liette Laflamme, Luc Gaudreau

引用次数: 0

Standardized Outcome Measures for the Clinical Application of Tissue Engineered Products. 组织工程产品临床应用的标准化结果测量。

Methods in molecular biology Pub Date : 2025-01-01 DOI: 10.1007/978-1-0716-4510-9_26

Anna S van den Bosch, Melinda Farkas, Frederique M Kemme, Matthea M Stoop, Paul P M van Zuijlen, Annebeth Meij-de Vries, Esther Middelkoop, Anouk Pijpe

{"title":"Standardized Outcome Measures for the Clinical Application of Tissue Engineered Products.","authors":"Anna S van den Bosch, Melinda Farkas, Frederique M Kemme, Matthea M Stoop, Paul P M van Zuijlen, Annebeth Meij-de Vries, Esther Middelkoop, Anouk Pijpe","doi":"10.1007/978-1-0716-4510-9_26","DOIUrl":"https://doi.org/10.1007/978-1-0716-4510-9_26","url":null,"abstract":"The clinical application of tissue engineered products aims to regenerate skin and enhance the appearance, texture, sensation, and functionality of affected skin or scars. To evaluate the efficacy and effectiveness of novel products, level 1 randomized controlled trials are required. However, currently a significant challenge in this field is the clinical and statistical heterogeneity of trial designs and outcome measures for tissue engineered products. To address this challenge and improve clinical outcomes, it is essential to standardize high-quality outcome measures. These measures are clinimetrically developed tools designed to reliably and validly assess specific health outcomes across diverse patient populations, clinical settings, and studies. This chapter proposes a core set of outcome measurements designed to evaluate the most relevant, feasible, reliable, and valid parameters in the clinical application of tissue engineered products. For routine clinical practice, we recommend the inclusion of photography, graft take, re-epithelialization, and scar quality assessment using the Patient and Observer Scar Assessment Scale. For clinical trials, additional measures such as colorimetry and elasticity assessment are advised. Detailed protocols for these methods are provided, resulting in a standardized core outcome set. High-quality outcome assessment also necessitates specialized training and the presence of trained personal at all assessment points. The chapter further addresses specific considerations for outcome assessment in pediatric patients, remote assessment strategies, and additional recommendations for optimizing clinical and research practices in this field. The implementation of standardized outcome measurement is essential for improving the outcomes of patients treated with tissue engineered products.","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":"2922 ","pages":"335-354"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flow Cytometry as Immunoassay Tool for Research on Yellow Fever Virus. 流式细胞术作为研究黄热病病毒的免疫分析工具。

Methods in molecular biology Pub Date : 2025-01-01 DOI: 10.1007/978-1-0716-4458-4_1

Raquel Curtinhas de Lima, Nieli Rodrigues da Costa Faria, Amanda Torrentes de Carvalho

{"title":"Flow Cytometry as Immunoassay Tool for Research on Yellow Fever Virus.","authors":"Raquel Curtinhas de Lima, Nieli Rodrigues da Costa Faria, Amanda Torrentes de Carvalho","doi":"10.1007/978-1-0716-4458-4_1","DOIUrl":"https://doi.org/10.1007/978-1-0716-4458-4_1","url":null,"abstract":"Flow cytometry is a sensitive and practical technique that can be applied in both basic and clinical research. It allows extracting quantitative and multiparametric valuable information to assist in the study of cell immunophenotyping, morphological complexity, location and/or expression of extra and intracellular molecules involved in metabolic and proliferative pathways, inflammation, viability, and cell death, among others. The parameters are analyzed by labeling antigens of yellow fever virus and/or cells with fluorescent specific monoclonal antibodies or dyes for immunophenotyping and data acquisition using a flow cytometer. In translational research, flow cytometry is a useful tool in tracking and monitoring the etiology, evolution, and outcome of several infectious diseases, such as yellow fever (YF), with the aim of benefiting human health through detection of intracellular viral antigen and vaccine efficacy trials, as well as characterization, quantification, and monitoring of immune cells subpopulations and their biological functions quality. In the last 10 years we have been facing the re-emergence of YF, considered an endemic disease caused by arbovirus in continents including South America. Unfortunately, severe forms of the disease are still associated with increased mortality. Even with the availability of effective vaccines, gaps about understanding the immune pathophysiology and clinical management are still considered a huge challenge for the scientific community. Therefore, such tool has the potential to aggregate in flavivirus setting, being effective in tracking infection in different biological culture systems, animal and human models, as well as in searching for new antiviral drugs and vaccine efficacy.","PeriodicalId":18490,"journal":{"name":"Methods in molecular biology","volume":"2913 ","pages":"1-17"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144044066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0