ELISA for Detection of Total IgG Against YF 17DD.

Q4 Biochemistry, Genetics and Molecular Biology

Methods in molecular biology Pub Date : 2025-01-01 DOI:10.1007/978-1-0716-4458-4_3

Ana Carolina R A Cajaraville

引用次数: 0

Abstract

The ELISA technique has attracted widespread interest as a sensitive, efficient, safe, and inexpensive way to measure antigen-antibody reactions since its description in 1971 to current days. Here it is described an indirect ELISA protocol to detect anti-YF 17DD antibodies as an important tool to evaluate immunogenicity to yellow fever vaccine candidates.

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ELISA检测YF - 17DD总IgG。

自1971年被描述至今，ELISA技术作为一种敏感、高效、安全和廉价的抗原抗体反应测量方法引起了广泛的兴趣。本文描述了一种间接ELISA检测抗yf 17DD抗体的方案，作为评估黄热病候选疫苗免疫原性的重要工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Methods in molecular biology Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

2.00

自引率

0.00%

发文量

3536

期刊介绍： For over 20 years, biological scientists have come to rely on the research protocols and methodologies in the critically acclaimed Methods in Molecular Biology series. The series was the first to introduce the step-by-step protocols approach that has become the standard in all biomedical protocol publishing. Each protocol is provided in readily-reproducible step-by-step fashion, opening with an introductory overview, a list of the materials and reagents needed to complete the experiment, and followed by a detailed procedure that is supported with a helpful notes section offering tips and tricks of the trade as well as troubleshooting advice.