Medical Research Journal最新文献

{"title":"Icaritin induces apoptosis and downregulates RhoA/ROCK pathway in the A549 adenocarcinoma cell line","authors":"A. Żuryń, Adrian Krajewski","doi":"10.5603/mrj.2022.0045","DOIUrl":"https://doi.org/10.5603/mrj.2022.0045","url":null,"abstract":"Introduction: Icaritin is a prenylflavonoid derivative from a traditional Chinese herb, Epimedium Genus. Icaritin exerts its anti-tumour effects in various cancers mainly by inhibiting cell proliferation, inducing cell differentiation and apoptosis, and suppressing cell migration and invasion. The study aimed to evaluate the effect of icaritin on apoptosis, cell cycle, and expression of selected cell cycle proteins: RhoA, ROCK1, and ROCK2 in the A549 cell line. Methods: Apoptosis and the cell cycle distribution were evaluated by flow cytometry. Protein expression was measured by conducting a western blot assay and immunofluorescence staining. Results and conclusions: The study revealed that icaritin induces apoptosis and cell cycle arrest in the G2/M phase in A549 cells. The authors showed that icaritin activates the p53 protein and downregulates Rho/ROCK pathway. Additionally, the decrease in expression of thrombospondin-1 was observed which can be part of the cytoprotective response of A549 cells on ICT treatment. The present study investigates the antitumour prop-erties of icaritin and conducts molecular analysis to show that ICT exerts its effect via the RhoA/ROCK pathway.","PeriodicalId":18485,"journal":{"name":"Medical Research Journal","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82643909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical markers of oxidative stress in patients with inflammatory bowel diseases","authors":"G. Mierzwa, J. Budzyński, D. Kupczyk, B. Augustyńska","doi":"10.5603/mrj.a2022.0044","DOIUrl":"https://doi.org/10.5603/mrj.a2022.0044","url":null,"abstract":"Introduction: Inflammatory bowel disease (IBD) is a group of diseases of unexplained aetiology, charac - terized by periods of remissions and exacerbations. Reactive oxygen species (ROS) as far as disorders of balance between levels of prooxidants and antioxidants may also participate in the occurrence of IBD. The aim of the present study was an assessment of the antioxidative barrier of the organism in patients with inflammatory bowel disease. Material and methods: The study group consisted of 99 patients (80 with IBD as a study group and 19 healthy as a control group) from Jan Biziel University Hospital in Bydgoszcz, Poland. Venous blood was the material for biochemical analysis: HT, GSH, GPX p , GPX RBC , GST, GR, SOD-1, MDA, NO 2/NO 3-and CP. Results: There were statistically significant differences in oxidative stress parameters observed between the study group and the control group, especially concerning HT, GSH, GPX RBC , GST, SOD-1, MDA and NO 2/NO 3. Discussion: The assumption that increased activity of antioxidative compounds may constitute a defence against the influence of oxidative stress may be true. Their decreased activity may participate in lowering an organism’s abilities to defend against oxidative stress and cause the development of free radical diseases. Further studies into targeted preventive strategies are needed. Conclusions: Prooxidative factors play an essential role in the pathogenesis of IBD. Due to the still un - known etiopathology of IBD, research on imbalances between pro-oxidants and antioxidants should be continued in larger groups of patients.","PeriodicalId":18485,"journal":{"name":"Medical Research Journal","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90714261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intranasal vaccines: a panacea to vaccine hesitancy?","authors":"M. J. Oladoye","doi":"10.5603/mrj.a2022.0043","DOIUrl":"https://doi.org/10.5603/mrj.a2022.0043","url":null,"abstract":"","PeriodicalId":18485,"journal":{"name":"Medical Research Journal","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85045913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of the COVID-19 pandemic on alcohol-related emergency department visits in a large European city","authors":"Hanna Cholerzyńska, Wiktoria Zasada, Patryk Konieczka, T. Kłosiewicz","doi":"10.5603/mrj.a2022.0042","DOIUrl":"https://doi.org/10.5603/mrj.a2022.0042","url":null,"abstract":"","PeriodicalId":18485,"journal":{"name":"Medical Research Journal","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85432368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment of vulvar lichen sclerosus with a fractional CO2 laser — case report","authors":"Kamila Stopińska, Adrianna Marzec, A. Olejek, Tomasz Niebudek","doi":"10.5603/mrj.a2022.0041","DOIUrl":"https://doi.org/10.5603/mrj.a2022.0041","url":null,"abstract":"","PeriodicalId":18485,"journal":{"name":"Medical Research Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91264196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Participation of fire protection units in Poland in ensuring the continuous operation of ventilators for home use — a 7-year observation","authors":"Łukasz Dudziński, Marcin Glinka, T. Kubiak, Mariusz Feltynowski","doi":"10.5603/mrj.a2022.0038","DOIUrl":"https://doi.org/10.5603/mrj.a2022.0038","url":null,"abstract":"Introduction: After each weather front with gales all over Poland, firemen have to perform a few hundred or so interventions. The life of persons covered by a specialistic treatment — home mechanical ventilation HMV — depends upon stable supplies of electricity. In case of breakdowns, indispensable aid is given by firemen who deliver generating sets available in Rescue and Firefighting Units (RFU). Aim of the study: Analysis and a statistical presentation of interventions of fire protection units to support the correct operation of home medical machines in case of temporary power failure. Material and methods: Data obtained from the Decision Support System of the State Fire Service (SFS DSS), made available to the authors by the Operational Planning Bureau at the Headquarters of the State Fire Service. In graphical presentations utilized were materials from the Head Office of Geodesy and Cartography, Law on Geodesy and Cartography, viz. Database for Topographic Objects, National Register of Borders. Medical data consistent with the purpose of the study were collected from the analysis of reports compiled by the person in charge of a rescue action of the State Fire Service, which is not the leading service in medical interventions. Results: In 2015–2021 were 1490 local hazards in which case it was necessary to support the operation — in most cases of ventilators for home use among people requiring ventilator-based therapy. The data show a constantly rising trend, the number of such actions increased by 64.29% — from 163 to 276 [mean (M) = 215.86, standard deviation (SD) = 66.35]. The average waiting time for undertaking inter - vention in these types of events was 910.68 seconds (15 min, 10 s). Conclusions: A significant element of rescue actions are volunteer fire brigade squads for being better deployed in rural areas, provided with generating sets; they can ensure faster an emergency electricity source. Persons in charge of a rescue action should draw up the medical part of a report more accurately (concerning the victim and the disease entity) while performing qualified first aid procedures.","PeriodicalId":18485,"journal":{"name":"Medical Research Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89759730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The clinical relevance assessment of ROSIER scale in emergency care","authors":"Dawid Woszczyk, T. Kłosiewicz","doi":"10.5603/mrj.a2022.0040","DOIUrl":"https://doi.org/10.5603/mrj.a2022.0040","url":null,"abstract":"","PeriodicalId":18485,"journal":{"name":"Medical Research Journal","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77753624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of knowledge on the functioning of patients with coronary artery disease","authors":"P. Michalski, M. Kasprzak, A. Kosobucka-Ozdoba, Łukasz Pietrzykowski, Magadalena Kieszkowska, A. Bączkowska, A. Kubica","doi":"10.5603/mrj.a2022.0039","DOIUrl":"https://doi.org/10.5603/mrj.a2022.0039","url":null,"abstract":"","PeriodicalId":18485,"journal":{"name":"Medical Research Journal","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81160598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the associations between GDF-15, OPG, and IL-15 levels in CRC: preliminary results","authors":"Julia Robotycka, Sylwia Mielcarska, Miriam Dawidowicz, A. Kula, Błażej Ochman, Tomasz Niebudek, Bogumiła Strzałkowska, D. Waniczek, E. Świętochowska","doi":"10.5603/mrj.a2022.0037","DOIUrl":"https://doi.org/10.5603/mrj.a2022.0037","url":null,"abstract":"","PeriodicalId":18485,"journal":{"name":"Medical Research Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85333502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The level of knowledge of healthcare professionals about child restraint systems","authors":"Ilona Skitek-Adamczak, Natalia Ciepluch, T. Kłosiewicz","doi":"10.5603/mrj.a2022.0036","DOIUrl":"https://doi.org/10.5603/mrj.a2022.0036","url":null,"abstract":"","PeriodicalId":18485,"journal":{"name":"Medical Research Journal","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88472271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}