Methods in molecular medicine最新文献_第8页

Monocyte-derived dendritic cells as antigen-presenting cells in T-cell proliferation and cytokine production. 单核细胞来源的树突状细胞在t细胞增殖和细胞因子产生中的抗原呈递细胞作用。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-366-0_9

Sun-sang J Sung

引用次数: 11

Inhibition of chaperone-dependent bacterial ribosome biogenesis. 伴侣依赖细菌核糖体生物发生的抑制作用。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-246-5_7

Abdalla Al Refaii, Jean-Hervé Alix

引用次数: 3

A method to assay penicillin-binding proteins. 一种测定青霉素结合蛋白的方法。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-246-5_11

Michael J Pucci, Thomas J Dougherty

引用次数: 3

Bacterial efflux pump inhibitors. 细菌外排泵抑制剂。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-246-5_15

Barbara J Kamicker, Michael T Sweeney, Frank Kaczmarek, Fadia Dib-Hajj, Wenchi Shang, Kim Crimin, Joan Duignan, Thomas D Gootz

{"title":"Bacterial efflux pump inhibitors.","authors":"Barbara J Kamicker, Michael T Sweeney, Frank Kaczmarek, Fadia Dib-Hajj, Wenchi Shang, Kim Crimin, Joan Duignan, Thomas D Gootz","doi":"10.1007/978-1-59745-246-5_15","DOIUrl":"https://doi.org/10.1007/978-1-59745-246-5_15","url":null,"abstract":"Infections caused by multidrug-resistant Gram-negative pathogens play a major role in the morbidity and mortality of hospitalized patients. The rise of resistance to current antibiotic therapies has made the discovery of new agents urgent. One of the major antibiotic resistance mechanisms utilized by more than 15 species of Gram-negative bacterial cells is the Resistance Nodulation Division (RND) efflux pump, which eliminates several classes of antibiotics such as penicillins and cephalosporin macrolides aminoglycosides, fluoroquinolonesx and tetracyclines. Here we describe a multistep process to identify compounds that inhibit the RND-type efflux pumps. This involves measuring the inhibition of accumulation of ethidium bromide in E. coli or Haemophilus influenzae cells and confirming that the inhibition is specific for the efflux pumps by using genetic constructs and biochemical methods to measure nonspecific inhibition due to e.g. intrinsic antibacterial activity or membrane disruption. In whole bacterial cells synergism antagonism or indifference of the combination of an antibiotic with the putative inhibitor is determined and this is then confirmed by quantitating viable bacterial cells in liquid culture over 24 h.","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":"142 ","pages":"187-204"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-59745-246-5_15","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27404788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 31

Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase III (mtFabH) assay: principles and method. 结核分枝杆菌-酮酰酰基载体蛋白合成酶III (mtFabH)测定:原理和方法。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-246-5_16

Sarbjot Sachdeva, Kevin A Reynolds

{"title":"Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase III (mtFabH) assay: principles and method.","authors":"Sarbjot Sachdeva, Kevin A Reynolds","doi":"10.1007/978-1-59745-246-5_16","DOIUrl":"https://doi.org/10.1007/978-1-59745-246-5_16","url":null,"abstract":"Fatty acid biosynthesis is one of the relatively newer targets in antibacterial drug discovery. The presence of distinct fatty acid synthases (FAS) in mammals and bacteria and the fact that most bacterial FAS enzymes are essential for viability make this a very attractive antimicrobial drug target. The enzyme beta-ketoacyl ACP synthase (KASIII or FabH) is the key enzyme that initiates fatty acid biosynthesis in a type II dissociated FAS. This enzyme catalyzes the condensation of acyl CoA and malonyl ACP (acyl carrier protein) to form a beta-ketoacyl ACP product, which is further processed to form mature fatty acids that are involved in various essential cellular processes and structures like phospholipid biosynthesis, cell wall formation, etc. Herein we describe a new assay for the Mycobacterium tuberculosis FabH (mtFabH) enzyme involved in a key initiation step in the synthesis of mycolic acids, which are an integral component of the cell wall. The assay eliminates the need for the cumbersome washing steps or specialty scintillation proximity assay beads and the preparation of acyl carrier proteins required in other assay formats. This discontinuous assay involves the reduction of radiolabled long-chain beta-ketoacyl CoA product to its dihydroxy derivative, which partitions into a nonpolar phase for quantitation, while the reduced radiolabeled substrate derivative remains in the aqueous phase.","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":"142 ","pages":"205-13"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-59745-246-5_16","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27404789","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Growth and purification of enteric adenovirus type 40. 肠道腺病毒40型的生长和纯化。

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1385/1-59745-166-5:145

Vivien Mautner

引用次数: 7

Adenovirus capsid chimeras: fiber terminal exon insertions/gene replacements in the major late transcription unit. 腺病毒衣壳嵌合体:主要晚期转录单元中的纤维末端外显子插入/基因替换。

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1385/1-59745-166-5:107

Jason Gall, John Schoggins, Erik Falck-Pedersen

引用次数: 1

Molecular profiling of hematopoietic stem cells. 造血干细胞的分子分析。

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1007/978-1-59745-223-6_1

Teresa V Bowman, Akil A Merchant, Margaret A Goodell

引用次数: 9

Sequence-specific primed PCR (PCR-SSP) typing of HLA Class I and Class II alleles. HLAⅰ类和ⅱ类等位基因序列特异性引物PCR分型研究。

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1007/978-1-59745-223-6_4

Klara Dalva, Meral Beksac

引用次数: 11

Identification of bone marrow derived nonhematopoietic cells by double labeling with immunohistochemistry and in situ hybridization. 免疫组织化学和原位杂交双重标记鉴定骨髓来源的非造血细胞。

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1007/978-1-59745-223-6_11

Isinsu Kuzu, Meral Beksac

{"title":"Identification of bone marrow derived nonhematopoietic cells by double labeling with immunohistochemistry and in situ hybridization.","authors":"Isinsu Kuzu, Meral Beksac","doi":"10.1007/978-1-59745-223-6_11","DOIUrl":"https://doi.org/10.1007/978-1-59745-223-6_11","url":null,"abstract":"Stem cell migration/trafficking is a field of interest that is shared by pathologists, histologists, clinical transplantation teams, cardiologists, neurologists, and many other members of different disciplines. Until the findings of a successful combination of in situ methods, the origin of chimeric parenchymal cells was a dilemma. These doublelabeling techniques have brought insight to our new concept of stem cell biology. It has been extremely helpful in the detection of the origin of terminally differentiated, including hematopoietic and nonhematopoietic, cells appearing following allogeneic stem cell transplantation. It has also become a standard approach for evaluation of repopulation following tissue injury in solid organ transplant patients or experimental models. Although very useful, this technique has its advantages and pitfalls. It requires expertise in application and interpretation. Suitable selection of specific markers against parenchymal cells and preferably a cocktail of antibodies targeting infiltrating inflammatory cells are mandatory. One pitfall of this method is its restriction to sex-mismatched pairs. The spectrum of labels for X and Y chromosomes are suitable for combination. To prevent misinterpretation, the precautions needed are defined in this chapter.","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":"134 ","pages":"145-59"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26860959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0