Methods in molecular medicine最新文献_第9页

Cartilage histomorphometry. 软骨histomorphometry。

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1007/978-1-59745-401-8_9

Ernst B Hunziker

引用次数: 0

Immunohistochemistry of the inflamed synovium. 炎症滑膜的免疫组织化学。

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1007/978-1-59745-401-8_3

Martina Gogarty, Oliver Fitzgerald

引用次数: 3

Laser capture as a tool for analysis of gene expression in inflamed synovium. 激光捕获作为分析炎症滑膜基因表达的工具。

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1007/978-1-59745-401-8_6

Ulf Müller-Ladner, Martin Judex, Elena Neumann, Steffen Gay

引用次数: 1

Purification and mass spectrometry identification of microtubule-binding proteins from Xenopus egg extracts. 爪蟾卵提取物微管结合蛋白的纯化及质谱鉴定。

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1007/978-1-59745-442-1_3

Vincent Gache, Patrice Waridel, Sylvie Luche, Andrej Shevchenko, Andrei V Popov

{"title":"Purification and mass spectrometry identification of microtubule-binding proteins from Xenopus egg extracts.","authors":"Vincent Gache, Patrice Waridel, Sylvie Luche, Andrej Shevchenko, Andrei V Popov","doi":"10.1007/978-1-59745-442-1_3","DOIUrl":"https://doi.org/10.1007/978-1-59745-442-1_3","url":null,"abstract":"Microtubule-binding proteins are conveniently divided into two large groups: MAPs (microtubule-associated proteins), which can stabilize, anchor, and/or nucleate microtubules, and motors, which use the energy of ATP hydrolysis for a variety of functions, including microtubule network organization and cargo transportation along microtubules. Here, we describe the use of Taxol-stabilized microtubules for purification of MAPs, motors, and their complexes from Xenopus egg extracts. Isolated proteins are analysed using sodium dodecyl sulfate gel electrophoresis and identified by various mass spectrometry and database mining technologies. Found proteins can be grouped into three classes: (1) known MAPs and motors; (2) proteins previously reported as associated with the microtubule cytoskeleton, but without a clearly defined cytoskeletal function; (3) proteins not yet described as having microtubule localization. Sequence-similarity methods employed for protein identification allow efficient identification of MAPs and motors from species with yet unsequenced genomes.","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":"137 ","pages":"29-43"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2750007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27122678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Artificial skin. 人造皮肤。

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1007/978-1-59745-443-8_10

M Föhn, H Bannasch

引用次数: 0

Synthetic hydrogel matrices for guided bladder tissue regeneration. 用于引导膀胱组织再生的合成水凝胶基质。

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1007/978-1-59745-443-8_7

Catharina A M Adelöw, Peter Frey

{"title":"Synthetic hydrogel matrices for guided bladder tissue regeneration.","authors":"Catharina A M Adelöw, Peter Frey","doi":"10.1007/978-1-59745-443-8_7","DOIUrl":"https://doi.org/10.1007/978-1-59745-443-8_7","url":null,"abstract":"Tissue engineering aims to provide a temporary scaffold for repair at the site of injury or disease that is able to support cell attachment and growth while synthesis of matrix proteins and reorganization take place. Although relatively successful, bladder tissue engineering suffers from the formation of scar tissue at the scaffold implant site partly due to the phenotypic switch of smooth muscle cells (SMCs) from a quiescent contractile phenotype to a synthetic proliferative phenotype, known as myofibroblast. We hypothesize that culturing human SMCs in enzymatically degradable poly(ethylene) glycol (PEG) hydrogels modified with integrin-binding peptides, and in co-culture with human urothelial cells (UCs), will offer some insight as to the required environment for their subsequent differentiation into quiescent SMCs. We have established protocols for isolation, culture, and characterization of human bladder UCs, SMCs, and fibroblasts and investigated co-culture conditions for SMCs and UCs. The optimal PEG hydrogel properties, promoting growth of these cells, have been investigated by varying the amounts of cell adhesion peptide, PEG, and crosslinker and examined using light and fluorescence microscopy. Furthermore, the cell organization within and on top of gels 14 days post seeding has been examined by histology and immunohistochemistry. We have investigated a co-culture model for UCs and SMCs integrated into PEG hydrogels, mimicking a section of the bladder wall for reconstructive purposes that also could contribute to the understanding of the underlying basic mechanisms of SMC differentiation.","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":"140 ","pages":"125-40"},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-59745-443-8_7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27122803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Simultaneous detection of adenovirus RNA and cellular proteins by fluorescent labeling in situ. 原位荧光标记法同时检测腺病毒RNA和细胞蛋白。

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1007/978-1-59745-277-9_5

Eileen Bridge

引用次数: 0

Generation of multicellular tumor spheroids by the hanging-drop method. 悬挂滴法生成多细胞肿瘤球体。

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1007/978-1-59745-443-8_8

Nicholas E Timmins, Lars K Nielsen

引用次数: 179

Detection of reactive oxygen species and nitric oxide in vascular cells and tissues: comparison of sensitivity and specificity. 血管细胞和组织中活性氧和一氧化氮的检测:敏感性和特异性的比较。

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1007/978-1-59745-571-8_20

Hua Cai, Sergey Dikalov, Kathy K Griendling, David G Harrison

引用次数: 55

Assessment of PI-3 kinase and Akt in ischemic heart diseases in diabetes. PI-3激酶和Akt在糖尿病缺血性心脏病中的作用

Methods in molecular medicine Pub Date : 2007-01-01 DOI: 10.1007/978-1-59745-571-8_22

Takashi Matsui, Amy J Davidoff

引用次数: 11