Methods in molecular medicine最新文献_第7页

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-366-0_3

Helga Kahlert

{"title":"Production of T-cell lines.","authors":"Helga Kahlert","doi":"10.1007/978-1-59745-366-0_3","DOIUrl":"https://doi.org/10.1007/978-1-59745-366-0_3","url":null,"abstract":"Allergen-specific T-cell lines established from allergic patients provide the opportunity of investigating T-cell functions at the poly- or oligoclonal level. T-cell lines are useful in determining the presence or absence of antigen-specific T-cell reactivity. However, to obtain detailed knowledge of the action of T cells with clearly defined features, for example epitope specificity or phenotype, T-cell clones are necessary.The frequency of allergen-specific T cells in peripheral blood mononuclear cells (PBMC) tends to be low and so stimulation of PBMC with single allergens often results in low allergen-specific reactivity or requires high doses of the allergen. In contrast, the stimulation of PBMC with whole allergen extract results in stronger reactivity because a greater spectrum of T-cell specificities is addressed. Therefore, for the investigation of polyclonal reactivity toward single allergens it is useful to establish T-cell lines, which represent an allergen-specific enrichment of T cells from the respective individual. These T cells are poly- or oligoclonal and might possess different epitope specificities. The method described here is based on experiences with human T-cell lines and clones specific for several allergens from grass pollens and tree pollens.","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":"138 ","pages":"31-41"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-59745-366-0_3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27522437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Biocomputational strategies for microbial drug target identification. 微生物药物靶标鉴定的生物计算策略。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-246-5_1

Kishore R Sakharkar, Meena K Sakharkar, Vincent T K Chow

{"title":"Biocomputational strategies for microbial drug target identification.","authors":"Kishore R Sakharkar, Meena K Sakharkar, Vincent T K Chow","doi":"10.1007/978-1-59745-246-5_1","DOIUrl":"https://doi.org/10.1007/978-1-59745-246-5_1","url":null,"abstract":"The complete genome sequences of about 300 bacteria (mostly pathogenic) have been determined, and many more such projects are currently in progress. The detection of bacterial genes that are non-homologous to human genes and are essential for the survival of the pathogen represent a promising means of identifying novel drug targets. We present a subtractive genomics approach for the identification of putative drug targets in microbial genomes and demonstrate its execution using Pseudomonas aeruginosa as an example. The resultant analyses are in good agreement with the results of systematic gene deletion experiments. This strategy enables rapid potential drug target identification, thereby greatly facilitating the search for new antibiotics. It should be recognized that there are limitations to this computational approach for drug target identification. Distant gene relationships may be missed since the alignment scores are likely to have low statistical significance. In conclusion, the results of such a strategy underscore the utility of large genomic databases for in silico systematic drug target identification in the post-genomic era.","PeriodicalId":18460,"journal":{"name":"Methods in molecular medicine","volume":"142 ","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/978-1-59745-246-5_1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27404355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Flow cytometry of bacterial membrane potential and permeability. 细菌膜电位和通透性的流式细胞术。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-246-5_14

Howard M Shapiro

引用次数: 50

Protein comparative sequence analysis and computer modeling. 蛋白质比较序列分析和计算机建模。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-60327-148-6_13

Brett D Hambly, Cecily E Oakley, Piotr G Fajer

引用次数: 0

Measuring the effects of genes and environment on complex traits. 测量基因和环境对复杂性状的影响。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-60327-148-6_4

Jennifer H Barrett

引用次数: 5

Microarrays for the study of viral gene expression during human cytomegalovirus latent infection. 人巨细胞病毒潜伏感染过程中病毒基因表达的微阵列研究。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-60327-148-6_9

Barry Slobedman, Allen K L Cheung

引用次数: 13

Standardization of allergen extracts. 过敏原萃取物标准化。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-366-0_12

Jørgen Nedergaard Larsen, Sten Dreborg

引用次数: 13

The facilitated antigen binding (FAB) assay--a protocol to measure allergen-specific inhibitory antibody activity. 促进抗原结合(FAB)测定——一种测量过敏原特异性抑制抗体活性的方案。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-366-0_21

James N Francis

引用次数: 10

Production of human T-cell clones. 人类t细胞克隆的生产。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-366-0_4

Adrienne Verhoef

引用次数: 2

Mapping of human T-cell epitopes of allergens. 人t细胞过敏原表位的定位。

Methods in molecular medicine Pub Date : 2008-01-01 DOI: 10.1007/978-1-59745-366-0_5

Thomas Zeiler, Tuomas Virtanen

引用次数: 5