Medical Principles and Practice最新文献

Takotsubo Syndrome Recurrence, a Trigger for Increased 30-Day Cardiovascular Mortality. Takotsubo综合征复发是30天心血管死亡率增加的诱因

IF 2.9 3区医学

Medical Principles and Practice Pub Date : 2025-04-01 DOI: 10.1159/000545544

Albert Topf, Moritz Mirna, Uta C Hoppe, Michael Lichtenauer, Lukas Motloch, Ravi Vazirani, Sabine Horn, Iván J Núñez-Gil

{"title":"Takotsubo Syndrome Recurrence, a Trigger for Increased 30-Day Cardiovascular Mortality.","authors":"Albert Topf, Moritz Mirna, Uta C Hoppe, Michael Lichtenauer, Lukas Motloch, Ravi Vazirani, Sabine Horn, Iván J Núñez-Gil","doi":"10.1159/000545544","DOIUrl":"https://doi.org/10.1159/000545544","url":null,"abstract":"Objectives: Recurrence of Takotsubo syndrome (TTS) has been reported with a rate up to 4 %. The effect of TTS recurrence on clinical outcomes has not been in the focus of studies so far.Methods: In our study, we enrolled 435 patients with TTS, of whom 14 patients had a recurrence. The inclusion interval for the index event was from 2015 to 2022 and patients were followed to categorize them in the group of TTS with or without the recurrence. TTS recurrences were registered until 06/2024. Blood samples, data on clinical presentation, precipitating factors, cardiovascular risk factors, medications, demographics, echocardiographic and electrocardiographic parameters were obtained. The 30-day -cardiovascular mortality (CV), the 30-day mortality and arrhythmia during hospitalization were evaluated.Results: Co-morbidities, ECG changes, pre-medication and symptoms were not significantly different between TTS patients with and without recurrence. Only the index left-ventricular ejection fraction (LVEF) of TTS patients with recurrence was significantly decreased (p = 0.046). In the case of outcome parameters, TTS patients with recurrence had a significantly, higher 30-day-CV mortality compared to those without recurrence (21.4 % vs 3.8 %, p = 0.001) with a 5.9-fold higher 30-day-CV mortality ((B(SE)= 1.94(0.70), p = 0.001)).Conclusion: In our study, the TTS recurrence was associated with 5.9-fold higher 30 day-CV mortality. Therefore, a more precise monitoring is necessary in this high-risk group. Whether only, the reduced LVEF is responsible for the increased 30-day-CV mortality, is an open issue and more factors can be assumed to play a role.","PeriodicalId":18455,"journal":{"name":"Medical Principles and Practice","volume":" ","pages":"1-14"},"PeriodicalIF":2.9,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143764412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Not Indolamine 2, 3 Dioxygenase Polymorphisms, But Low Levels of IDO And IDO2 Are Associated with Behçet's Syndrome. 与behet综合征相关的不是吲哚胺2,3双加氧酶多态性，而是低水平的IDO和IDO2

IF 2.9 3区医学

Medical Principles and Practice Pub Date : 2025-03-29 DOI: 10.1159/000545581

Ülkü Uçar, Yağmur Aydin Atalay, Güven Özkaya, Haluk Barbaros Oral

{"title":"Not Indolamine 2, 3 Dioxygenase Polymorphisms, But Low Levels of IDO And IDO2 Are Associated with Behçet's Syndrome.","authors":"Ülkü Uçar, Yağmur Aydin Atalay, Güven Özkaya, Haluk Barbaros Oral","doi":"10.1159/000545581","DOIUrl":"https://doi.org/10.1159/000545581","url":null,"abstract":"Objective: Behçet's syndrome (BS) is a multisystemic disorder with a complex genetic background. Indoleamine 2,3-dioxygenase (IDO) and IDO2, key enzymes in tryptophan metabolism, have immunomodulatory effects. Specific IDO and IDO2 polymorphisms may influence enzymatic activity. This study aimed to explore the association between IDO/IDO2 gene polymorphisms and BS susceptibility, and assess serum levels of IDO and IDO2 in relation to BS.Subjects and methods: Ninety patients with BS and 52 healthy controls were enrolled in this study. Predetermined single nucleotide polymorphisms (SNPs) were studied at specific gene loci for IDO and IDO2. Serum IDO and IDO2 levels were determined using ELISA.Results: No statistically significant differences were observed in the genotype and allele frequencies of IDO (rs7820268 and rs10108662) and IDO2 (rs4503083) between patients with BS and controls. Furthermore, no significant association was found between clinical findings and SNPs, except that the IDO rs7820268 CT genotype was significantly lower in patients with neurological involvement (0% vs 42%, p=0.026, OR=0.147, 95%CI=0.18-1.231). Serum levels of IDO and IDO2 were significantly lower in BS patients compared to controls (p<0.0000 and p<0.0001, respectively).Conclusion: Our research revealed that the serum IDO/IDO2 levels of BS were substantially lower than those in the control group. This finding has the potential to impact IDO activity and reduce immune tolerance. No correlation was observed between IDO/IDO2 polymorphisms and most clinical findings of BS. However, the IDO rs7820268 CT genotype was significantly reduced in neuro-Behçet's syndrome, suggesting a protective effect. Larger prospective trials are needed to further explore these findings.","PeriodicalId":18455,"journal":{"name":"Medical Principles and Practice","volume":" ","pages":"1-18"},"PeriodicalIF":2.9,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143753358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Risks of new-onset atrial fibrillation after coronary artery bypass grafting. 冠状动脉旁路移植术后新发心房颤动的风险。

IF 2.9 3区医学

Medical Principles and Practice Pub Date : 2025-03-25 DOI: 10.1159/000545376

Orhan Guvenc, Gamze Güvenç, Mesut Engin

引用次数: 0

Biopsychosocial Factors and Pain Hypervigilance should be considered in the Interpretation of Disease Activity in Systemic Sclerosis. 在解释系统性硬化症的疾病活动时，应考虑生物心理因素和疼痛过度警觉性。

IF 2.9 3区医学

Medical Principles and Practice Pub Date : 2025-03-21 DOI: 10.1159/000545343

Özlem Kiliç, Mehmet Nur Kaya, Muhammed Canbaş, Muhammet Çınar, Sedat Yılmaz

{"title":"Biopsychosocial Factors and Pain Hypervigilance should be considered in the Interpretation of Disease Activity in Systemic Sclerosis.","authors":"Özlem Kiliç, Mehmet Nur Kaya, Muhammed Canbaş, Muhammet Çınar, Sedat Yılmaz","doi":"10.1159/000545343","DOIUrl":"https://doi.org/10.1159/000545343","url":null,"abstract":"Objective: This study aimed to identify a pain phenotype associated with hypervigilance in systemic sclerosis (SSc) and to evaluate possible variables that influence pain hypervigilance symptoms.Methods: This cross-sectional, observational study included healthy controls (HCs) and SSc patients diagnosed with a score of 9 or higher according to the 2013 American College of Rheumatology-European League Against Rheumatism classification criteria. The pain hypervigilance symptoms were evaluated using the CS Inventory (CSI) questionnaire, while disease activity was assessed using the European Scleroderma Research Group Activity Index (EScSG-AI). The patients were classified based on CSI scores. Comparative analyses were conducted for clinical, sociodemographic, and biopsychosocial factors.Results: 51 SSc patients (92.2% female, mean age 50.54±13.16 years) and 45 HCs (88.9% female, mean age 52.62±10.4 years) were included. Education and monthly income were lower for SSc than HCs (p<0.05). The CSI score≥40 proportion was 56.9% in SSc and 15.6% in HCs (p<0.001). Depression-Anxiety-Stress Scale (DASS-21), Epworth Sleepiness Scale (ESS), Global Pittsburgh Sleep Quality Index (PSQI), and EuroQol Five-Dimensional Three-Level Questionnaire (EQ-5D-3L) scores were higher in SSc than HCs (p<0.05). In multiple linear regression analysis to determine predictors of CSI score≥40, the effective variable was EScSG-AI. In multivariate logistic regression analysis, educational level and global PSQI scores were factors associated with CSI score≥40 in SSc.Conclusions: CSI score was positively associated with depression, disease activity, stress, anxiety, and poor sleep quality, and negatively associated with education and economic status. Pain hypervigilance may affect organ involvement and functioning in SSc. Clinicians should examine its biopsychosocial components.","PeriodicalId":18455,"journal":{"name":"Medical Principles and Practice","volume":" ","pages":"1-22"},"PeriodicalIF":2.9,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical and Medical Economic Value of Screening Colonoscopy before Laparoscopic Cholecystectomy. 腹腔镜胆囊切除术前筛查结肠镜的临床和医疗经济价值。

IF 2.9 3区医学

Medical Principles and Practice Pub Date : 2025-03-18 DOI: 10.1159/000545322

Tsuyoshi Igami, Masanao Nakamura, Takuya Ishikawa, Takeshi Yamamura, Kentaro Yamao, Keiko Maeda, Yasuyuki Mizutani, Tsunaki Sawada, Yukihiro Yokoyama, Takashi Mizuno, Junpei Yamaguchi, Shunsuke Onoe, Masaki Sunagawa, Nobuyuki Watanabe, Taisuke Baba, Shoji Kawakatsu, Hiroki Kawashima, Tomoki Ebata

{"title":"Clinical and Medical Economic Value of Screening Colonoscopy before Laparoscopic Cholecystectomy.","authors":"Tsuyoshi Igami, Masanao Nakamura, Takuya Ishikawa, Takeshi Yamamura, Kentaro Yamao, Keiko Maeda, Yasuyuki Mizutani, Tsunaki Sawada, Yukihiro Yokoyama, Takashi Mizuno, Junpei Yamaguchi, Shunsuke Onoe, Masaki Sunagawa, Nobuyuki Watanabe, Taisuke Baba, Shoji Kawakatsu, Hiroki Kawashima, Tomoki Ebata","doi":"10.1159/000545322","DOIUrl":"https://doi.org/10.1159/000545322","url":null,"abstract":"Objective: Clinical value of screening colonoscopy (SC) has been widely accepted; however, its clinical utility remains controversial in patients who undergo laparoscopic cholecystectomy (LC). The aim of this study is to evaluate the clinical value of medical care costs for SC before LC.Subject and methods: Of the 509 patients who underwent LC, 335 underwent preoperative SC before LC. The electronic medical records were retrospectively reviewed, and the technical fees of SC and endoscopic and/or surgical resection for colorectal neoplasia (CRN) were analyzed.Results: In the 335 patients with SC before LC, the rate of CRN requiring resection, including advanced adenoma and adenocarcinoma, were 13.1%. The detected rate of CRN requiring resection in the age groups of <45, 44-55, 55-65, 65-75, ≥75 years were 5.3%, 3.8%, 9.8%, 17.4%, and 22.9%, respectively. Of the 174 patients without SC before LC, four patients were diagnosed with resectable colorectal carcinomas after LC. The total technical fees of SC and/or treatment of CRNs among the 335 patients with SC before LC and surgical procedures among the four patients with resectable colorectal carcinoma were 84,700 United States dollar (USD) and 32,000 USD, respectively. Regarding the technical fee per person, the former group (250 USD) had much economic advantage compared to the latter group (8,000 USD).Conclusions: Scheduling LC is recognized as an important chance to undergo SC. For the patients aged ≥55 years, colonoscopy is no longer a screening option but a clinical necessity due to the high detected rates of CRN requiring resection.","PeriodicalId":18455,"journal":{"name":"Medical Principles and Practice","volume":" ","pages":"1-16"},"PeriodicalIF":2.9,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effectiveness of Hormonal Therapy for Post-Menopausal Women with Hormone Receptor-Positive Advanced Breast Cancer: A Systematic Review and Meta-Analysis of Clinical Trials. 激素治疗绝经后妇女激素受体阳性晚期乳腺癌的有效性：临床试验的系统回顾和荟萃分析。

IF 2.9 3区医学

Medical Principles and Practice Pub Date : 2025-03-17 DOI: 10.1159/000545264

Vitalis C Okwor, Juliet C Okwor, Maryjane K Ukwuoma, Sara B Mitha, Martins C Nweke

{"title":"Effectiveness of Hormonal Therapy for Post-Menopausal Women with Hormone Receptor-Positive Advanced Breast Cancer: A Systematic Review and Meta-Analysis of Clinical Trials.","authors":"Vitalis C Okwor, Juliet C Okwor, Maryjane K Ukwuoma, Sara B Mitha, Martins C Nweke","doi":"10.1159/000545264","DOIUrl":"10.1159/000545264","url":null,"abstract":"Objective: Breast cancer (BC) cells exhibit mutations over time, conferring resistance to therapeutic approaches. We attempted to ascertain the efficacy of selected hormonal therapy for advanced BC.Methods: This is a systematic review and meta-analysis of clinical trials. We searched Medline, PubMed, Cochrane Library, Web of Science and others. Studies that investigated the effectiveness of hormonal therapy for HR+ advanced BC were included. The outcomes were progression-free survival (PFS), overall survival (OS) and objective response rate (ORR). A random-effect meta-analysis model was employed. The study protocol was registered with the International Prospective Register of Systematic Reviews: CRD42023431939.Results: 21 studies were included in the meta-analysis with an overall sample size of 8482. ORR and PFS between aromatase inhibitors (AI) and other hormonal therapies: selective estrogen receptor degrader (SERD), selective estrogen modulator (SERM) and androgen inhibitors showed no significant difference [OR = 1.122 (0.917-1.374), p = 0.263]; [OR = 0.010 (0.000-1.292), p = 0.063], respectively. Sub-group analysis showed a statistically significant difference in ORR in favour of patients who received SERM compared to AI [OR = 1.362 (1.033-1.795), p = 0.028]. For OS, no significant difference was observed among anastrozole, letrozole and exemestane recepients [OR = 1.718 (0.021-139.128), p = 0.809].Conclusion: Given the above findings, clinical decisions could be based on factors such as the line of cancer treatment, adverse events, drug dosing, and individual drug benefits. Although newer combination therapies are being adopted, the agents explored in this review are still widely used in clinical practice for HR+ BC.","PeriodicalId":18455,"journal":{"name":"Medical Principles and Practice","volume":" ","pages":"1-26"},"PeriodicalIF":2.9,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Response to the Letter on "Risks of new-onset atrial fibrillation after coronary artery bypass grafting". 对“冠状动脉旁路移植术后新发房颤风险”函件的回应。

IF 2.9 3区医学

Medical Principles and Practice Pub Date : 2025-03-12 DOI: 10.1159/000545222

Sameh Alagha, Serkan Mola, Mehmet Çeber, Alp Yıldırım

引用次数: 0

Surveillance of Corrected QT Interval-Prolonging Medications upon Admission throughout Hospitalization in a Tertiary Care Geriatric Ward. 三级护理老年病房住院期间qtc间期延长药物的监测

IF 2.9 3区医学

Medical Principles and Practice Pub Date : 2025-02-20 DOI: 10.1159/000544820

Ivana Baralić Knežević, Milena Kovačević, Katarina Stefanović, Predrag Erceg, Gordana Mihajlović, Jovana Aćimović, Katarina M Vučićević

{"title":"Surveillance of Corrected QT Interval-Prolonging Medications upon Admission throughout Hospitalization in a Tertiary Care Geriatric Ward.","authors":"Ivana Baralić Knežević, Milena Kovačević, Katarina Stefanović, Predrag Erceg, Gordana Mihajlović, Jovana Aćimović, Katarina M Vučićević","doi":"10.1159/000544820","DOIUrl":"10.1159/000544820","url":null,"abstract":"Objective: Long QT syndrome (LQTS) poses a significant risk of torsade de pointes, particularly in older patients due to age-related changes in cardiac repolarization and increased susceptibility to medication-induced QTc interval prolongation. Despite the increased risk, data on medication-related LQTS remain limited, leading to this study on its prevalence, characteristics, and risk factors, along with QT-prolonging drug use in older patients. The study aimed to identify clinical and medication-related predictors of LQTS and evaluate the burden of co-prescribed QT-prolonging medications in this population.Subjects and methods: This prospective study at a tertiary care hospital included initial and follow-up ECGs, with medication details were collected. Statistical analyses compared variables, including QTc intervals and medication use, between patients with and without LQTS.Results: The study included 128 adults aged 65 or older, with 27.3% presenting LQTS on admission, increasing to 42.2% after 7 days of hospitalization. Patients with LQTS had a higher prevalence of QTc interval-prolonging medications, list 1 medications, and atrial fibrillation. Laboratory changes and medication use were observed, with significant increases in QTc interval and list 1 medication administration. Male sex and amiodarone use were identified as predictors of LQTS during hospitalization.Conclusion: The study reports a high prevalence of prolonged QTc interval and LQTS in older inpatients. Proton pump inhibitors were frequently prescribed despite their QTc-prolonging potential. This underscores the need of close monitoring and awareness of QTc prolongation risks in older patients, advocating for routine ECG assessments and vigilant management of modifiable risk factors, especially the electrolytes.","PeriodicalId":18455,"journal":{"name":"Medical Principles and Practice","volume":" ","pages":"1-10"},"PeriodicalIF":2.9,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143468531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vitamin D Supplementation Mediates a Shift toward Anti-Inflammatory Cytokine Response in Multiple Sclerosis. 补充维生素D介导多发性硬化症向抗炎细胞因子反应的转变。

IF 2.9 3区医学

Medical Principles and Practice Pub Date : 2025-02-12 DOI: 10.1159/000544106

Suhail Al-Shammri, Arpita Chattopadhyay, Raj Raghupathy

{"title":"Vitamin D Supplementation Mediates a Shift toward Anti-Inflammatory Cytokine Response in Multiple Sclerosis.","authors":"Suhail Al-Shammri, Arpita Chattopadhyay, Raj Raghupathy","doi":"10.1159/000544106","DOIUrl":"10.1159/000544106","url":null,"abstract":"Objective: In this study, we assessed the impact of vitamin D (Vit-D) supplementation on cytokine profile in newly diagnosed drug-naïve multiple sclerosis (MS) patients.Methods: We recruited 16 patients with incident MS; those were followed up for 12 months. Serum levels of 25-hydroxy-Vit-D were measured at recruitment and follow-up. Peripheral blood mononuclear cells were isolated from blood samples and stimulated with a mitogen in tissue culture to elicit cytokine production. Culture supernatants were tested after 4 days post-culture for levels of pro-inflammatory (TNF-α, interferon [IFN]-gamma, interleukin [IL]-6, IL-12) and anti-inflammatory cytokines (IL-4, IL-5, IL-10).Results: Baseline blood sample analysis revealed that patients had low Vit-D levels (16.8 [12.41-31.4] nmol/L). Thirteen patients expressed a normal intact parathyroid hormone (iPTH) (5.5 [4.2-6.9]) and were given Caltrate D twice a day; 3 patients having elevated iPTH (mean ± SD: 12.8 ± 1.5) were prescribed 50,000 IU Vit-D once weekly and Caltrate D twice daily. IFN-β1α was prescribed after a confirmed diagnosis of relapsing-remitting MS in 13 patients; 3 patients declined immunotherapy. Irrespective of immunotherapy, we observed a gradual and significant decrease in pro-inflammatory cytokine levels and a steady significant decline in ratios of several pro-inflammatory to anti-inflammatory cytokines. The patients with higher pro-inflammatory to anti-inflammatory cytokine ratios were more prone to having relapses.Conclusions: Low Vit-D levels and elevated pro-inflammatory cytokines in drug-naïve MS patients suggest a potential immune-pathogenic role for hypovitaminosis D in MS development. Vit-D supplementation may exert protective effects by modulating the immune-inflammatory response, emphasizing its therapeutic importance in MS management.","PeriodicalId":18455,"journal":{"name":"Medical Principles and Practice","volume":" ","pages":"1-9"},"PeriodicalIF":2.9,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histamine Promotes Pseudomonas aeruginosa Biofilm Formation and Renders P. aeruginosa Biofilms More Resistant to Gentamicin and Azithromycin. 组胺促进铜绿假单胞菌生物膜的形成并使铜绿假单胞菌生物膜对庆大霉素和阿奇霉素更耐药。

IF 2.9 3区医学

Medical Principles and Practice Pub Date : 2025-02-12 DOI: 10.1159/000544111

Karim Dib

{"title":"Histamine Promotes Pseudomonas aeruginosa Biofilm Formation and Renders P. aeruginosa Biofilms More Resistant to Gentamicin and Azithromycin.","authors":"Karim Dib","doi":"10.1159/000544111","DOIUrl":"10.1159/000544111","url":null,"abstract":"Introduction: Pseudomonas aeruginosa biofilms contribute to the persistent presence of this bacterium in the cystic fibrosis airways. P. aeruginosa produces histamine in vitro and expresses histamine receptors. We investigated whether histamine regulated P. aeruginosa biofilm formation in vitro and contributed to bacterial virulence in Galleria mellonella.Methods: P. aeruginosa biofilms were measured by staining bacteria adhered on polystyrene with crystal violet. Histamine concentrations were measured by ELISA. G. mellonella survival upon inoculation with P. aeruginosa was measured in the absence or presence of histamine.Results: The concentration of histamine in the BHI broth was 140 ng/mL (1.3 μ<sc>m</sc>). Addition to the broth of diamine oxidase (DAO), an enzyme that catabolizes histamine, reduced by ∼3-fold the concentration of histamine and by 2-fold PAO1 strain biofilms. Addition of histamine (10-9<sc>m</sc>-10-4<sc>m</sc>) to the LB medium augmented P. aeruginosa biofilms. Maximum effects were observed with concentrations of 10-5<sc>m</sc> and 10-8<sc>m</sc> for the mucoid NH57388A strain and the PAO1 strain, respectively. DAO reduced mucoid NH57388A biofilms induced by histamine (10-4<sc>m</sc>) added to the LB medium. Addition of histamine to 48 h formed biofilms reduced anti-biofilm activities of gentamicin and azithromycin. Inoculation of G. mellonella with the PAO1 strain led to augmented histamine concentration in the haemolymph. Inoculation of histamine (10-8<sc>m</sc>) reduced the survival rate of G. mellonella infected with the PAO1 strain.Conclusion: Histamine produced during periods of infection may augment P. aeruginosa virulence by promoting the biofilm mode of life of this bacterium.","PeriodicalId":18455,"journal":{"name":"Medical Principles and Practice","volume":" ","pages":"1-12"},"PeriodicalIF":2.9,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143408716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0