Microbiome最新文献

{"title":"Deciphering the coordinated roles of the host genome, duodenal mucosal genes, and microbiota in regulating complex traits in chickens.","authors":"Fangren Lan, Xiqiong Wang, Qianqian Zhou, Xiaochang Li, Jiaming Jin, Wenxin Zhang, Chaoliang Wen, Guiqin Wu, Guangqi Li, Yiyuan Yan, Ning Yang, Congjiao Sun","doi":"10.1186/s40168-025-02054-5","DOIUrl":"10.1186/s40168-025-02054-5","url":null,"abstract":"<p><strong>Background: </strong>The complex interactions between host genetics and the gut microbiome are well documented. However, the specific impacts of gene expression patterns and microbial composition on each other remain to be further explored.</p><p><strong>Results: </strong>Here, we investigated this complex interplay in a sizable population of 705 hens, employing integrative analyses to examine the relationships among the host genome, mucosal gene expression, and gut microbiota. Specific microbial taxa, such as the cecal family Christensenellaceae, which showed a heritability of 0.365, were strongly correlated with host genomic variants. We proposed a novel concept of regulatability ( <math><msubsup><mi>r</mi> <mrow><mi>b</mi></mrow> <mn>2</mn></msubsup> </math> ), which was derived from h<sup>2</sup>, to quantify the cumulative effects of gene expression on the given phenotypes. The duodenal mucosal transcriptome emerged as a potent influencer of duodenal microbial taxa, with much higher <math><msubsup><mi>r</mi> <mrow><mi>b</mi></mrow> <mn>2</mn></msubsup> </math> values (0.17 ± 0.01, mean ± SE) than h<sup>2</sup> values (0.02 ± 0.00). A comparative analysis of chickens and humans revealed similar average microbiability values of genes (0.18 vs. 0.20) and significant differences in average <math><msubsup><mi>r</mi> <mrow><mi>b</mi></mrow> <mn>2</mn></msubsup> </math> values of microbes (0.17 vs. 0.04). Besides, cis ( <math><msubsup><mi>h</mi> <mrow><mtext>cis</mtext></mrow> <mn>2</mn></msubsup> </math> ) and trans heritability ( <math><msubsup><mi>h</mi> <mrow><mtext>trans</mtext></mrow> <mn>2</mn></msubsup> </math> ) were estimated to assess the effects of genetic variations inside and outside the cis window of the gene on its expression. Higher <math><msubsup><mi>h</mi> <mrow><mtext>trans</mtext></mrow> <mn>2</mn></msubsup> </math> values than <math><msubsup><mi>h</mi> <mrow><mtext>cis</mtext></mrow> <mn>2</mn></msubsup> </math> values and a greater prevalence of trans-regulated genes than cis-regulated genes underscored the significant role of loci outside the cis window in shaping gene expression levels. Furthermore, our exploration of the regulatory effects of duodenal mucosal genes and the microbiota on 18 complex traits enhanced our understanding of the regulatory mechanisms, in which the CHST14 gene and its regulatory relationships with Lactobacillus salivarius jointly facilitated the deposition of abdominal fat by modulating the concentration of bile salt hydrolase, and further triglycerides, total cholesterol, and free fatty acids absorption and metabolism.</p><p><strong>Conclusions: </strong>Our findings highlighted a novel concept of <math><msubsup><mi>r</mi> <mrow><mi>b</mi></mrow> <mn>2</mn></msubsup> </math> to quantify the phenotypic variance attributed to gene expression and emphasize the superior role of intestinal mucosal gene expressions over host genomic variations in elucidating host‒microbe interactions for comp","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"62"},"PeriodicalIF":13.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11871680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143537166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roux-en-Y gastric bypass-associated fecal tyramine promotes colon cancer risk via increased DNA damage, cell proliferation, and inflammation.","authors":"Maria Glymenaki, Sophie Curio, Smeeta Shrestha, Qi Zhong, Laura Rushton, Rachael Barry, Mona El-Bahrawy, Julian R Marchesi, Yulan Wang, Nigel J Gooderham, Nadia Guerra, Jia V Li","doi":"10.1186/s40168-025-02049-2","DOIUrl":"10.1186/s40168-025-02049-2","url":null,"abstract":"<p><strong>Background: </strong>Fecal abundances of Enterobacteriaceae and Enterococcaceae are elevated in patients following Roux-en-Y gastric bypass (RYGB) surgery. Concurrently, fecal concentrations of tyramine, derived from gut bacterial metabolism of tyrosine and/or food, increased post-RYGB. Furthermore, emerging evidence suggests that RYGB is associated with increased colorectal cancer (CRC) risk. However, the causal link between RYGB-associated microbial metabolites and CRC risk remains unclear. Hence, this study investigated the tyrosine metabolism of Enterobacteriaceae and Enterococcaceae strains isolated from patients post-RYGB and explored the causal effects of tyramine on the CRC risk and tumorigenesis using both human colonic cancer cell line (HCT 116) and wild-type and Apc^Min/+ mice.</p><p><strong>Results: </strong>We isolated 31 bacterial isolates belonging to Enterobacteriaceae and Enterococcaceae families from the feces of patients with RYGB surgery. By culturing the isolates in tyrosine-supplemented medium, we found that Citrobacter produced phenol as a main product of tyrosine, whereas Enterobacter and Klebsiella produced 4-hydroxyphenylacetate, Escherichia produced 4-hydroxyphenyllactate and 4-hydroxyphenylpyruvate, and Enterococcus and two Klebsiella isolates produced tyramine. These observations suggested the gut bacterial contribution to increased fecal concentrations of tyramine post-RYGB. We subsequently evaluated the impact of tyramine on CRC risk and development. Tyramine induced necrosis and promoted cell proliferation and DNA damage of HCT 116 cells. Daily oral administration of tyramine for 49 days to wild-type mice resulted in visible adenomas in 5 out of 12 mice, accompanied by significantly enhanced DNA damage (γH2AX +) and an increased trend of cell proliferation (Ki67 +) in the ileum, along with an upregulated expression of the cell division cycle gene (Cdc34b) in the colon. To evaluate the impact of tyramine on intestinal tumor growth, we treated Apc^Min/+ mice with the same doses of tyramine and duration. These mice showed larger colonic tumor size and increased intestinal cell proliferation and inflammation (e.g., increased mRNA expression of IL-17A and higher number of Ly6G + neutrophils) compared to water-treated Apc^Min/+ control mice.</p><p><strong>Conclusions: </strong>Our results collectively suggested that RYGB-associated fecal bacteria could contribute to tyramine production and tyramine increased CRC risk by increasing DNA damage, cell proliferation, and pro-inflammatory responses of the gut. Monitoring and modulating tyramine concentrations in high-risk individuals could aid CRC prognosis and management. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"60"},"PeriodicalIF":13.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869571/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An in vitro model demonstrating homeostatic interactions between reconstructed human gingiva and a saliva-derived multispecies biofilm.","authors":"Lin Shang, Sanne Roffel, Vera Slomka, Eleanor M D'Agostino, Aline Metris, Mark J Buijs, Bernd W Brandt, Dongmei Deng, Susan Gibbs, Bastiaan P Krom","doi":"10.1186/s40168-025-02033-w","DOIUrl":"10.1186/s40168-025-02033-w","url":null,"abstract":"<p><strong>Background: </strong>In the oral cavity, host-microbe interactions (HMI) continuously occur and greatly impact oral health. In contrast to the well-studied disease-associated HMI during, for example, periodontitis, HMI that are essential in maintaining oral health have been rarely investigated, especially in a human-relevant context. The aim of this study was to extensively characterize homeostatic HMI between saliva-derived biofilms and a reconstructed human gingiva (RHG). RHG was reconstructed following the structure of native gingiva, composed of a multilayered epithelium formed by keratinocytes and a fibroblast-populated compartment. To mimic the oral environment, RHG were inoculated with pooled human saliva resuspended in different saliva substitute media and incubated for 2 or 4 days. The co-cultured biofilms were retrieved and characterized by viable bacterial counting and compositional profiling (16S rRNA gene sequencing). RHG was investigated for metabolic activity (MTT assay), tissue histology (hematoxylin and eosin staining), epithelial proliferation (Ki67 staining), antimicrobial peptide expression, and cytokine secretion.</p><p><strong>Results: </strong>Viable biofilms were detected up to day 4 of co-culturing. Bacterial counts indicated biofilm growth from the inoculation to day 2 and maintained thereafter at a similar level until day 4. All biofilms shared similar composition throughout 4 days, independent of co-culture time and different saliva substitute media used during inoculation. Biofilms were diverse with Streptococcus, Haemophilus, and Neisseria being the dominating genera. While supporting biofilm development, RHG displayed no significant changes in metabolic activity, tissue histology, or epithelial proliferation. However, in the presence of biofilms, the antimicrobial peptides elafin and human β-defensin-2 were upregulated, and the secretion of cytokines IL-6, CXCL1, CXCL8, CCL5, and CCL20 increased.</p><p><strong>Conclusion: </strong>This model mimicked homeostatic HMI where a healthy gingiva supported a viable, diverse, and stable microbial community, incorporating bacterial genera found on native gingiva. The gingiva model maintained its tissue integrity and exerted protective responses in the presence of biofilms over time. This study adds to the evidence that shows the important role of the host in maintaining homeostatic HMI that are essential for oral health. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"58"},"PeriodicalIF":13.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Virome specific to tick genus with distinct ecogeographical distribution.","authors":"Di Tian, Run-Ze Ye, Yu-Yu Li, Ning Wang, Wan-Ying Gao, Bai-Hui Wang, Zhe-Tao Lin, Wen-Jie Zhu, Qiu-Shi Wang, Ya-Ting Liu, Hua Wei, Yi-Fei Wang, Yi Sun, Xiao-Yu Shi, Na Jia, Jia-Fu Jiang, Xiao-Ming Cui, Wu-Chun Cao, Zhi-Hong Liu","doi":"10.1186/s40168-025-02061-6","DOIUrl":"10.1186/s40168-025-02061-6","url":null,"abstract":"<p><strong>Background: </strong>The emergence of tick-borne pathogens poses a serious threat to both human and animal health. There remains controversy about virome diversity in relation to tick genus and ecogeographical factors.</p><p><strong>Results: </strong>We conducted the meta‑transcriptomic sequencing of 155 pools of ticks encompassing 7 species of 3 genera collected from diverse geographical fauna of Ningxia Province, China. Two species of Dermacentor genus were distributed in the predominantly grassland areas of the central and eastern regions, with the lowest viral diversity. Two species of Hyalomma ticks were found in the predominantly desert areas of the northern regions, with intermediate viral diversity. Three species of Haemaphysalis ticks were concentrated in the predominantly forested areas of the southern regions, exhibiting the highest viral diversity. We assembled 348 viral genomes of 63 species in 14 orders, including 26 novel viruses. The identified viruses were clearly specific to tick genus: 22 virus species were exclusive to Dermacentor, 12 to Hyalomma, and 27 to Haemaphysalis.</p><p><strong>Conclusions: </strong>The associations between tick genera and geographical distribution, viral richness, and composition provide new insights into tick-virus interactions, offering clues to identify high-risk regions for different tick-borne viruses. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"57"},"PeriodicalIF":13.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiota-derived urolithin A in monoclonal gammopathies and multiple myeloma therapy.","authors":"Alba Rodríguez-García, Raquel Ancos-Pintado, Roberto García-Vicente, Alejandra Ortiz-Ruiz, Andrés Arroyo, Miguel Ángel Navarro, María Luz Morales, Patricia Guevara-Ramirez, Pablo Justo, Nieves López-Muñoz, José Sánchez-Pina, Rafael Alonso, María Victoria Selma, María Dolores Frutos-Lisón, Rocío García-Villalba, Francisco A Tomás-Barberán, Rosa Ayala, Joaquín Martínez-López, María Linares","doi":"10.1186/s40168-025-02045-6","DOIUrl":"10.1186/s40168-025-02045-6","url":null,"abstract":"<p><strong>Background: </strong>Gut microbiota-derived urolithins may influence multiple myeloma (MM) disease progression and treatment. We analyzed urolithins and their associated microbiota in a retrospective cohort of 45 patients with active MM or premalignant disease using mass spectrometry and 16S rRNA gene sequencing.</p><p><strong>Results: </strong>Patients with detectable levels of urolithin in serum and stool and a higher abundance of urolithin-related microbiota had a better outcome. Analysis of the effects of urolithin A (UroA) treatment ex vivo, in vitro, and in vivo revealed that UroA is cytotoxic against MM cell lines and modulates the cell cycle and mitochondrial activity. Notably, UroA inhibits the proliferation of primary MM cells in vitro and in a xenograft mouse model, improving overall survival. Finally, combination therapy with UroA and bortezomib has a synergistic effect in vitro, even in the presence of bortezomib resistance, and modulates signaling pathways involved in MM development.</p><p><strong>Conclusions: </strong>UroA might be a potential therapeutic agent to halt MM disease progression or to overcome resistance when used in combination. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"56"},"PeriodicalIF":13.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869585/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enterocloster clostridioformis protects against Salmonella pathogenesis and modulates epithelial and mucosal immune function.","authors":"Benjamin S Beresford-Jones, Satoshi Suyama, Simon Clare, Amelia Soderholm, Wangmingyu Xia, Puspendu Sardar, Junhee Lee, Katherine Harcourt, Trevor D Lawley, Virginia A Pedicord","doi":"10.1186/s40168-025-02050-9","DOIUrl":"10.1186/s40168-025-02050-9","url":null,"abstract":"<p><strong>Background: </strong>Promoting resistance to enteric pathogen infection is a core function of the gut microbiota; however, many of the specific host-commensal interactions that mediate this protection remain uncharacterised. To address this knowledge gap, we monocolonised germ-free mice with mouse-derived commensal microbes to screen for microbiota-induced resistance to Salmonella Typhimurium infection.</p><p><strong>Results: </strong>We identified Enterocloster clostridioformis as a protective species against S. Typhimurium infection. E. clostridioformis selectively upregulates resistin-like molecule β and cell cycle pathway expression at the level of caecal epithelial cells and increases T-regulatory cells in the underlying mucosal immune system, potentially contributing to reduced infection-induced pathology.</p><p><strong>Conclusions: </strong>We highlight novel mechanisms of host-microbe interactions that can mediate microbiota-induced resistance to acute salmonellosis. In the backdrop of increasing antibiotic resistance, this study identifies novel potential avenues for further research into protective host responses against enteric infections and could lead to new therapeutic approaches. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"61"},"PeriodicalIF":13.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shared environments complicate the use of strain-resolved metagenomics to infer microbiome transmission.","authors":"Reena Debray, Carly C Dickson, Shasta E Webb, Elizabeth A Archie, Jenny Tung","doi":"10.1186/s40168-025-02051-8","DOIUrl":"10.1186/s40168-025-02051-8","url":null,"abstract":"<p><strong>Background: </strong>In humans and other social animals, social partners have more similar microbiomes than expected by chance, suggesting that social contact transfers microorganisms. Yet, social microbiome transmission can be difficult to identify based on compositional data alone. To overcome this challenge, recent studies have used information about microbial strain sharing (i.e., the shared presence of highly similar microbial sequences) to infer transmission. However, the degree to which strain sharing is influenced by shared traits and environments among social partners, rather than transmission per se, is not well understood.</p><p><strong>Results: </strong>Here, we first use a fecal microbiota transplant dataset to show that strain sharing can recapitulate true transmission networks under ideal settings when donor-recipient pairs are unambiguous and recipients are sampled shortly after transmission. In contrast, in gut metagenomes from a wild baboon population, we find that demographic and environmental factors can override signals of strain sharing among social partners.</p><p><strong>Conclusions: </strong>We conclude that strain-level analyses provide useful information about microbiome similarity, but other facets of study design, especially longitudinal sampling and careful consideration of host characteristics, are essential for inferring the underlying mechanisms of strain sharing and resolving true social transmission network. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"59"},"PeriodicalIF":13.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11869744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Evolutionary diversification and succession of soil huge phages in glacier foreland.","authors":"Hu Liao, Jian Li, Yan-Zi Wang, Hu Li, Xin-Li An, Tao Wang, Rui-Ying Chang, Yong-Guan Zhu, Jian-Qiang Su","doi":"10.1186/s40168-025-02068-z","DOIUrl":"10.1186/s40168-025-02068-z","url":null,"abstract":"","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"55"},"PeriodicalIF":13.8,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11866690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Live Akkermansia muciniphila boosts dendritic cell retinoic acid synthesis to modulate IL-22 activity and mitigate colitis in mice.","authors":"Hongbin Liu, Ruo Huang, Binhai Shen, Chongyang Huang, Qian Zhou, Jiahui Xu, Shengbo Chen, Xinlong Lin, Jun Wang, Xinmei Zhao, Yandong Guo, Xiuyun Ai, Yangyang Liu, Ye Wang, Wendi Zhang, Fachao Zhi","doi":"10.1186/s40168-025-02060-7","DOIUrl":"10.1186/s40168-025-02060-7","url":null,"abstract":"","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"54"},"PeriodicalIF":13.8,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11837666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Aerobic anoxygenic phototrophs play important roles in nutrient cycling within cyanobacterial Microcystis bloom microbiomes.","authors":"Haiyuan Cai, Christopher J McLimans, Helong Jiang, Feng Chen, Lee R Krumholz, K David Hambright","doi":"10.1186/s40168-025-02056-3","DOIUrl":"10.1186/s40168-025-02056-3","url":null,"abstract":"","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"53"},"PeriodicalIF":13.8,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829547/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}