Microbiome最新文献

{"title":"Novel Antarctic chemolithotroph drives iron biomineralization.","authors":"Jaekyung Yoon, Boyoung Lee, Kyu-Cheul Yoo, Min-Jung Kwak, Hae Jung Song, Chung Yeon Hwang, Yusook Chung, Kitae Kim, Soon-Kyeong Kwon, Ju Yeon Song, Hwan Su Yoon, Jihyun F Kim","doi":"10.1186/s40168-026-02536-0","DOIUrl":"10.1186/s40168-026-02536-0","url":null,"abstract":"<p><strong>Background: </strong>Iron, the most abundant redox-active metal in the Earth's crust, is coupled to numerous biogeochemical cycles. However, the mechanisms of iron oxidation and the organisms involved remain incompletely understood. Banded iron formations (BIFs) are a major reservoir of iron ore in the Precambrian sedimentary record, yet the biological contribution to their genesis remains a subject of unresolved debate. While large-scale BIF deposition largely ceased after the Proterozoic, microbial activity in modern Holocene sediments under fluctuating redox conditions provides a unique opportunity to examine mechanisms reminiscent of ancient iron cycling. Here, we report the stratigraphic sequence of microbiome profiles recorded in laminated facies with iron-containing crystalline illite in the embayment sediments beneath the Larsen C Ice Shelf (LCIS) in Antarctica during the Holocene.</p><p><strong>Results: </strong>LCIS sediments record microbial community shifts tightly coupled to environmental changes throughout the Holocene. Metagenomic analyses revealed three dominant microbial phases corresponding to geological facies boundaries. The open marine setting (phase A) showed higher taxonomic richness, whereas the sub-ice shelf sediments (phases B and C) were largely anoxic and characterized by diverse chemolithoautotrophic metabolisms. Keystone taxa including uncultured members of Thermodesulfovibrionia, as well as unique microbial communities and metabolisms, were evident in the aphotic, anoxic seawater; metagenomic analyses further revealed chemolithotrophy. The Thermodesulfovibrionia bacterium, visualized using fluorescence in situ hybridization and designated as \"Candidatus Mariimomonas ferrooxydans\", formed a novel clade in the phylum Nitrospirota. Metagenome-assembled genome analysis identified a putative outer-membrane Fe(II) oxidase, Cyc2, whose Fe(II)-oxidation activity was experimentally confirmed.</p><p><strong>Conclusion: </strong>Our findings document the interaction between microbiome and environment, illustrating how LCIS sediments preserve a dynamic record of microbial community responses to environmental transitions. These results provide critical insights into microbial iron mineralization, echoing the biogeochemistry of the geologic past, particularly synglacial iron formation during the Neoproterozoic Snowball Earth. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"14 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13647500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149128045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lung microbiota dysbiosis exacerbates influenza virus infection.","authors":"Hai Chang Yin, Xin Yu Zhang, Song Liu, Xinjie Jiang, Tian Fei Yu, He Zhang, Di Liu, Chang You Xia","doi":"10.1186/s40168-026-02450-5","DOIUrl":"10.1186/s40168-026-02450-5","url":null,"abstract":"<p><strong>Background: </strong>Previous studies have shown that microbiota in the respiratory tract play a significant role in shaping host immunity and reducing the risk of respiratory tract infections. Influenza, an acute respiratory infectious disease caused by the influenza virus (IAV), necessitates to have an in-depth understanding of the pathogenic mechanisms of viral infection and to utilize these mechanisms to prevent and control influenza infection.</p><p><strong>Results: </strong>In this study, we established models of different disease severity levels in mice infected with IAV, the relationships between changes in pulmonary microbiota and host interactions during IAV infection were analyzed. We found that these relationships were characterized by the enrichment of opportunistic pathogenic taxonomic groups and were related to disease severity. Multi-omics analysis combined with animal experiments clarified that colonization of Bacillus cereus in the lungs aggravates IAV infection through xanthosine, thereby downregulating the expression of nt5e and reducing the viral phagocytic ability of alveolar macrophages. Importantly, targeted removal of B. cereus by bacteriophages can alleviate IAV infection and the resulting pulmonary lesions.</p><p><strong>Conclusions: </strong>Highlighting provides a novel approach for preventing and controlling the progression of IAV infection by targeting the lung microbiota.</p><p><strong>In brief: </strong>Lung dysbiosis is associated with severe influenza virus (IAV) infection. Bacillus cereus aggravates IAV infection via the xanthosine-nt5e axis in SPF mice, which can be rescued by bacteriophage targeting. These findings highlight the potential of modulating the lung microbiota as a strategy for prevention and controlling IAV infection.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"14 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13651400/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Porphyromonas gingivalis induces intestinal inflammation through gingipain-dependent gut microbiome dysbiosis.","authors":"Ming Li, Jiyun Cui, Ru Qu, Rulong Liu, Yifan Sun, Ping Li, Juan Liu, Adrian Low, Xiaochang Huang, Fei Gan, Zhenjiang Zech Xu","doi":"10.1186/s40168-026-02532-4","DOIUrl":"10.1186/s40168-026-02532-4","url":null,"abstract":"","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"14 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2026-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543490/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148891914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Atg2 coordinates microbial metabolite signaling and epigenetic remodeling to maintain intestinal lipid homeostasis in Drosophila.","authors":"Ping Wang, Xinran Li, Jiangong Zhang, Jiewei Wang, Li Hua Jin","doi":"10.1186/s40168-026-02512-8","DOIUrl":"10.1186/s40168-026-02512-8","url":null,"abstract":"","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"14 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2026-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13528086/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148865616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat stress enhances gut microbial arginine catabolism to amplify MyD88-dependent inflammatory responses.","authors":"Xianxi Ye, Qingpeng Cai, Yangjie Pan, Minxuan Xu, Yulong Li, Minjie You, Jiayi Yang, Siyuan Chen, Huacheng He, Guangliang Hong, Hong Zheng","doi":"10.1186/s40168-026-02514-6","DOIUrl":"10.1186/s40168-026-02514-6","url":null,"abstract":"<p><strong>Background: </strong>As global temperatures continue to rise, heat stress (HS) has emerged as a major health threat of growing concern. HS triggers systemic inflammation and multi-organ damage, but so far its molecular mechanisms remain unclear. In this study, we explored the potential mechanism by which the gut microbial alterations amplify HS-associated inflammatory responses.</p><p><strong>Results: </strong>We found that the gut microbiota was disrupted in HS mice as characterized by increased LPS levels and enhanced arginine catabolism. Transplant of fecal microbiota from HS mice aggravated inflammatory responses in recipient mice after HS. Exogenous arginine pretreatment notably suppressed inflammation in the liver and cortex of HS mice. Mechanistically, arginine reduced MyD88 protein levels by activating its ubiquitination and weakened the MyD88-TLR4 interaction, thereby inhibiting the nuclear translocation of p65 and the expression of pro-inflammatory genes. Clinically, lower arginine levels were detected in the serum of HS patients and positively related with liver injury and inflammatory indicators. An arginine-enriched oral inulin hydrogel was developed to prevent inflammatory responses exacerbated by the gut microbial alterations through maintaining the gut microbiota homeostasis to reduce LPS and providing a sustained supply of arginine.</p><p><strong>Conclusions: </strong>This study reveals a mechanism by which the gut microbial alterations exacerbates HS-associated inflammatory responses via disrupting the balance between LPS and arginine, thereby providing a novel target for the prevention of HS. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"14 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2026-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13560437/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148930425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Baseline gut microbiome and metabolome profiles predict weight loss after a structured lifestyle intervention.","authors":"Benjamin Seethaler, Maryam Basrai, Nathalie M Delzenne, Jens Walter, Nguyen K Nguyen, Stephan C Bischoff","doi":"10.1186/s40168-026-02501-x","DOIUrl":"10.1186/s40168-026-02501-x","url":null,"abstract":"<p><strong>Background: </strong>Obesity remains a global health challenge, and responses to lifestyle-based weight-loss interventions are heterogeneous. Here, we evaluate a one-year structured lifestyle program in 50 adults with obesity (mean BMI 42 ± 7.0 kg/m²), integrating clinical, microbiome, and metabolomic profiling, to identify predictors of weight-loss success and metabolic improvement (ClinicalTrials.gov: NCT01344525). The intervention included a 3-month very low-calorie formula diet (approximately 850 kcal/day), a 3-month transition phase from a formula diet to a balanced diet (approximately 1000 kcal/day), and a 6-month maintenance period in which the participants followed a balanced diet (gradually increasing to a maximum of 2000 kcal/day).</p><p><strong>Results: </strong>Following the intervention, the participants exhibited marked reductions in body weight, body fat percentage, C-reactive protein, and glycated hemoglobin. Longitudinal analyses revealed that shifts in the gut microbiota composition were associated with changes in clinical and anthropometric data, as well as gut barrier function. An increased abundance of Lachnospiraceae was associated with improved gut barrier function; the relationship was mediated by fecal butyrate and propionate. Multivariate analyses revealed that serum baseline levels of diacylphosphatidylcholine C40:1 predicted postintervention BMI, indicating that this metabolite could serve as a biomarker of weight loss success. A random forest model incorporating baseline microbial and clinical features was used to predict weight loss and clinical improvements with high accuracy.</p><p><strong>Conclusions: </strong>Our findings elucidate the interplay between the gut microbiota and host metabolism during weight loss and highlight the potential utility of baseline profiling to achieve a high success rate in personalized obesity treatment. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"14 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2026-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13525661/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148857542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phagocytosis of microbial symbionts supports embryonic nutrition in the sponge Halichondria panicea.","authors":"Marta Turon, Cristina Díez-Vives, Tyler J Carrier, Laura de la Cruz-Castillejo, Maria Conejero, Leon Steiner, Sabrina Jung, Lucía Pita, Vasiliki Koutsouveli, Sören Franzenburg, Ute Hentschel, Ana Riesgo","doi":"10.1186/s40168-026-02473-y","DOIUrl":"10.1186/s40168-026-02473-y","url":null,"abstract":"<p><strong>Background: </strong>Animal development is frequently supported by microbial symbionts that contribute to host nutrition, metabolism, and physiology. While the functional importance of microbiomes in adult hosts is increasingly recognized, the role of symbiotic microbes during gametogenesis and embryogenesis remains poorly understood, particularly in early-diverging metazoans. Sponges represent an ideal system to investigate these processes due to their dense and diverse microbial communities. Here, we examined host-symbiont dynamics across the reproductive cycle of the marine sponge Halichondria panicea to assess how microbial communities contribute to reproduction and early development.</p><p><strong>Results: </strong>Specimens were collected monthly from February to July and classified by reproductive stage using histological analyses. We combined ultrastructural imaging, dual RNA sequencing of host and symbionts, 16S rRNA gene amplicon sequencing, and quantitative PCR to characterize microbial and transcriptional dynamics throughout reproduction. Pronounced shifts in both host gene expression and microbial community composition occurred during early embryogenesis, particularly in May. Transmission electron microscopy revealed nurse cells phagocytosing bacterial aggregates in close proximity to late oocytes, presumably converting them into yolk precursors. This coincided with a significant decline in the abundance of the dominant obligate symbiont, Candidatus Halichondribacter symbioticus. Host transcriptomic analyses showed upregulation of immune and phagocytic pathways, including pattern recognition receptors, lectins, and vesicle trafficking components, specifically in females undergoing embryogenesis in May. Concurrently, symbiont gene expression profiles indicated responses to acidic conditions, consistent with exposure to phagosomal environments.</p><p><strong>Conclusions: </strong>Our results are consistent with intracellular digestion of microbial symbionts during early embryogenesis, potentially to supplement the nutritional requirements of embryogenesis. These findings reveal symbiont phagocytosis as a previously underappreciated nutritional strategy during animal development and highlight the dynamic functional integration of microbiomes into reproductive physiology in basal metazoans. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"14 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2026-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13543439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148891922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering microbial community dynamics using cross-sectional data-informed NeuralODE.","authors":"Feng Xue, Xiaoxiu Tan, Chenhong Zhang, Hongyu Zhao, Tao Wang","doi":"10.1186/s40168-026-02458-x","DOIUrl":"10.1186/s40168-026-02458-x","url":null,"abstract":"<p><strong>Background: </strong>Understanding the ecological mechanisms of host-associated microbial ecosystems typically relies on either cross-sectional or time-series data. Cross-sectional analyses are limited in their ability to assess intervention effects, whereas time-series models require dense and informative sampling that is often impractical.</p><p><strong>Results: </strong>Here, we present an enhanced Neural Ordinary Differential Equations (NeuralODE) framework that, for the first time, integrates cross-sectional data into the dynamic modeling of sparse and weakly informative temporal data. We develop two instantiations of this framework, tailored to relative and absolute abundances, and introduce a dynamic keystoneness metric to quantify species importance over time. Across simulated and real-data benchmarks, incorporating cross-sectional data improved performance over competing methods, particularly in data-scarce settings. Moreover, biological validation demonstrated that the framework recovers experimentally supported interactions and prioritizes identified influential species.</p><p><strong>Conclusions: </strong>Together, these results establish our method as a reliable framework for mechanistic modeling of microbial ecosystems, offering new insights into their dynamic behavior. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"14 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13523204/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148840864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiome analysis and epigenetic patterns revealed distinct differences between two elm species with contrasting Dutch elm disease resistance.","authors":"Hans Hoenicka, Kristina Ulrich, Charlotte Haffner, Marta Starczak, Daniel Gackowski, Ben Bubner, Andreas Ulrich","doi":"10.1186/s40168-026-02488-5","DOIUrl":"10.1186/s40168-026-02488-5","url":null,"abstract":"<p><strong>Background: </strong>Dutch elm disease (DED), caused by the fungus Ophiostoma novo-ulmi, has devastated elm species in Europe and North America for over a century. While the Chinese elm (Ulmus parvifolia) frequently displays notable resistance to DED, the European wych elm (Ulmus glabra) remains highly susceptible. Understanding microbiome interaction and epigenetic factors might help to explain this contrasting resilience and subsequently improve disease management.</p><p><strong>Results: </strong>Seedlings of both elm species were germinated and maintained under identical environmental conditions. Comparative metabarcoding revealed distinct differences in bacterial and eukaryotic microbiomes, as well as in their associations with lichenized fungi and their photobiont algae. In U. parvifolia, 24 eukaryotic and 14 bacterial taxa were identified as the most differentially abundant microbiome components, and exhibited significantly different levels compared to U. glabra. In particular, Leotiomycetes fungi and Rhizobiales bacteria, both previously implicated in resistance to pathogens, were strongly enriched in U. parvifolia. Lichenized fungi and chlorophyte algae were likewise more abundant in U. parvifolia. In U. glabra, 11 eukaryotic and 16 bacterial taxa were significantly more abundant, including Nocardioides bacteria and Leptospora fungi. Ultra-performance liquid chromatography coupled with mass spectrometry revealed that, compared to U. glabra, U. parvifolia exhibited higher levels of 5-(hydroxymethyl)-2'-deoxycytidine and lower levels of 5-methyl-2'-deoxycytidine and N6-methyl-2'-deoxyadenosine in DNA, as well as increased amounts of 5-methylcytidine, N6-methyladenosine, and 5-hydroxymethyluridine in RNA. The elevated levels of well-established epigenetic markers in DNA and RNA are remarkable. However, it remains unclear what part of the holobiont may be responsible for this effect.</p><p><strong>Conclusions: </strong>The contrasting susceptibility of Chinese and European elms to DED correlates with pronounced differences in their associated microbial communities and lichens, as well as in patterns of epigenetic nucleoside modifications. In particular, the enrichment of specific stress-associated microbiome components, together with lichen associations and elevated levels of modified DNA and RNA nucleosides in U. parvifolia, may underlie its increased tolerance to DED. Although this study did not identify the factors responsible for DED resistance, it provides valuable insights into microbiomic and epigenetic features that could contribute to the development of new approaches to combat DED. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"14 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2026-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13488114/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148795201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conserved protein sequence-structure signatures identify antibiotic resistance genes from the human microbiome.","authors":"Lachlan Bartrop, Emy Beauchemin-Lauzon, Frédéric Grenier, Sébastien Rodrigue, Louis-Patrick Haraoui","doi":"10.1186/s40168-026-02487-6","DOIUrl":"10.1186/s40168-026-02487-6","url":null,"abstract":"<p><strong>Background: </strong>Bacteria exhibiting antimicrobial resistance (AMR) are a significant public health challenge worldwide. The human microbiome is a known repository of key determinants of AMR, antibiotic resistance genes (ARGs). Identifying previously undetected ARGs in the human microbiome that confer resistance to clinical concentrations of antibiotics is a crucial component of addressing AMR, yet can be hindered by their low homology to existing ARGs. Here, we address this by focussing on functionally important protein regions.</p><p><strong>Results: </strong>ARG-PASS (ARG-PAirwise Sequence vs Structure) is a protein function prediction method which leverages a one-class support vector machine trained on pairwise primary and tertiary distributions of structurally conserved regions of proteins encoded by ARGs. ARG-PASS was applied to six reference strains of the Human Microbiome Project. Nine candidates were selected for experimental verification, and all were functionally confirmed when expressed in E.coli, belonging to ARG classes: APH, dfr, class B and C β-lactamases, and penicillin-binding proteins. We also used ARG-PASS directly on protein structures within the AlphaFold database and identified a phnP gene (metallo-β-lactamase fold) with low similarity to characterised β-lactamases and media-dependent ampicillin activity. In total, 80% of the tested genes confer resistance at CLSI resistant breakpoints and the remainder represent 'pre-resistance' genes, with activity but not at a clinically relevant minimum inhibitory concentration. We suggest pre-resistance genes may preferentially evolve into clinically relevant resistance determinants.</p><p><strong>Conclusion: </strong>ARG-PASS provides a precise computational approach that integrates sequence and structure to identify divergent ARGs that evade homology-based detection, supporting improved resistance surveillance and antibiotic stewardship. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"14 1","pages":""},"PeriodicalIF":14.9,"publicationDate":"2026-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13602658/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149030822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}