Microbiome最新文献

{"title":"β-Elemonic acid mediated enrichment of Paenibacillus to help Salvia miltiorrhiza Bunge alleviate drought stress.","authors":"Hong-Mei Jia, Jie Zhou, Wen-Cheng Zhao, Dong-Mei He, Zhu-Yun Yan","doi":"10.1186/s40168-025-02154-2","DOIUrl":"https://doi.org/10.1186/s40168-025-02154-2","url":null,"abstract":"<p><strong>Background: </strong>Microorganisms can improve the adaptability of crops to drought and high-temperature stress. However, the changes of rhizosphere microbial communities under climate stress and the potential mechanisms driving microbial changes remain poorly understood.</p><p><strong>Results: </strong>In this study, the medicinal plant Salvia miltiorrhiza was used as the research object. ITS, 16S rRNA amplicon sequencing, and liquid chromatography-mass spectrometry-based metabolomics were integrated to investigate its physiological and biochemical responses to drought, high-temperature, and combined drought-high temperature under greenhouse. Additionally, we determined the seedling weight, leaf water content, active ingredient content of underground part, and the content of chlorophyll, leaf nitrogen, phosphorus, and potassium. The results demonstrated that microorganisms can alleviate stress by enhancing the water retention capacity of S. miltiorrhiza leaves; TD group increased by about 13%, promoting nutrient absorption; and the chlorophyll content of group D increased by about 78%, boosting photosynthetic efficiency and increasing the levels of stress-resistant compounds. We found that bacteria exhibited greater sensitivity to climatic stress factors, with Paenibacillus being significantly enriched only in the stress-treated group. Moreover, the synthetic community comprising Paenibacillus was confirmed to help S. miltiorrhiza alleviate drought stress. We further found that β-elemonic acid, a triterpene acid secreted by plant roots, specifically enriched Paenibacillus under drought stress. In addition, β-elemonic acid significantly promoted the growth of S. miltiorrhiza in the presence of Paenibacillus under drought stress.</p><p><strong>Conclusions: </strong>Our findings suggest that S. miltiorrhiza enrich beneficial Paenibacillus to combat drought stress through the secretion of the key metabolite β-elemonic acid. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"153"},"PeriodicalIF":13.8,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of hydrogen oxidation coupled with antimonate reduction, a novel antimony biogeochemical cycling, in two contrasting antimony-contaminated environments.","authors":"Yize Wang, Xiaoxu Sun, Yubo Cao, Zhimin Xu, Huicai Sun, Xiaojie Guan, Muhammad Usman Ghani, Lin Zheng, Baoqin Li, Duanyi Huang, Weimin Sun","doi":"10.1186/s40168-025-02149-z","DOIUrl":"https://doi.org/10.1186/s40168-025-02149-z","url":null,"abstract":"<p><strong>Background: </strong>Antimony (Sb) contamination is a serious environmental problem owing to its extensive production worldwide. High concentration of Sb is often detected in mining-contaminated environments, leading to the risk of contamination to the downstream environments through waterflow. Microorganisms play an important role in the fate and transport of Sb. Microbially mediated Sb(V) reduction performs an important environmental service because it can reduce the mobility of Sb and prevent the transport of Sb to downstream. As a commonly found intermediate in mining and aquatic environments, molecular hydrogen (H₂) may serve as an electron donor to drive Sb(V) reduction, although this biogeochemical process has not yet been reported.</p><p><strong>Results: </strong>In this study, Sb(V) reduction coupled with H₂ oxidation (HOSbR) was identified in two contrasting Sb-contaminated habitats, i.e., oligotrophic tailings and organic-rich river sediments. DNA-stable isotope probing identified Azospirillum and Hydrogenophaga spp. as the bacteria potentially responsible for HOSbR in oligotrophic tailings and organic-rich river sediments, respectively. Further, Azospirillum spp. were identified as keystone taxa in tailings. The causal inference framework suggested that Azospirillum spp. may contribute to the increased nitrogenase activity in oligotrophic tailings during HOSbR.</p><p><strong>Conclusions: </strong>These results suggest that bacteria responsible for HOSbR may play various important ecological roles including reducing the mobility of Sb and improving nutrient conditions in oligotrophic habitats. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"152"},"PeriodicalIF":13.8,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Wild wisdom meets cultivation: comparative rhizomicrobiome analysis unveils the key role of Paraburkholderia in growth promotion and disease suppression in Coptis chinensis.","authors":"Xianhe Cao, Qingjun Yuan, Chengcheng Hu, Hanxing Zhang, Xianyun Sun, Binbin Yan, Xiaojing Ma, Long Zhang, Luqi Huang, Shaojie Li, Zhenying Zhang","doi":"10.1186/s40168-025-02136-4","DOIUrl":"10.1186/s40168-025-02136-4","url":null,"abstract":"<p><strong>Background: </strong>The sustained monoculture and irregular planting practices rendered the cultivated Coptis chinensis more prone to various diseases compared to its wild counterparts. Rewilding the rhizomicrobiome of cultivated plants has emerged as a promising strategy to promote plant growth, but ancestral microbiota suitable for C. chinensis remain largely uncharted.</p><p><strong>Results: </strong>The amplicon data analyses revealed that habitat transition strongly influenced the rhizosphere microbial communities. The rhizomicrobiomes of wild C. chinensis encompassed a more diverse array of ecological groups and exhibited a greater functional diversity compared to their cultivated counterparts. A higher proportion of beneficial fungi was observed in the rhizosphere of wild C. chinensis, while the cultivated plants had a higher population of pathogenic fungi. Furthermore, a well-documented plant-growth-promoting rhizobacterium genus, Paraburkholderia, was found to play an essential role in the resistance of the wild C. chinensis to potential disease caused by Ilyonectria. Two strains of Paraburkholderia (Paraburkholderia nemoris and Paraburkholderia phytofirmans) were isolated, and in vitro experiments confirmed that these isolates possess various growth-promoting properties and antagonistic activities against known pathogens for C. chinensis root rot. Both of the Paraburkholderia isolates could markedly promote the plant immune response and enhance the overall health of the cultivated C. chinensis.</p><p><strong>Conclusions: </strong>By a comprehensive comparison of the rhizosphere microbiome between wild and cultivated C. chinensis, the promising bacterial genus Paraburkholderia was identified as a beneficial microbe significantly promoting the growth of C. chinensis, providing pivotal insights for future endeavors aimed at engineering the rhizosphere microbiome of C. chinensis, as well as other medicinal herbs. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"150"},"PeriodicalIF":13.8,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181905/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uncovering encrypted antimicrobial peptides in health-associated Lactobacillaceae by large-scale genomics and machine learning.","authors":"Rubing Du, Fei Han, Zhen Li, Jing Yu, Yan Xu, Yongguang Huang, Qun Wu","doi":"10.1186/s40168-025-02145-3","DOIUrl":"10.1186/s40168-025-02145-3","url":null,"abstract":"<p><strong>Background: </strong>Antimicrobial peptides (AMPs) are well known for their broad-spectrum activity and have shown great promise in addressing the antibiotic-resistant crisis. The Lactobacillaceae family, recognized for its health-promoting effects in humans, represents a valuable source of novel AMPs. However, the global prevalence and distribution of AMPs within Lactobacillaceae remains largely unknown, which limits the efficient discovery and development of novel AMPs.</p><p><strong>Results: </strong>We analyzed all available genomes (10,327 genomes), encompassing 38 genera and 515 species, to investigate the biosynthetic potential (indicated by the number of AMP sequences in the genome) of AMP in the Lactobacillaceae family. We demonstrated Lactobacillaceae species had ubiquitous (69.90%) biosynthetic potential of AMPs. Overall, 9601 AMPs were identified, clustering into 2092 gene cluster families (GCFs), which showed strong interspecies specificity (95.27%), intraspecies heterogeneity (93.31%), and habitat uniqueness (95.83%), that greatly expanded on the AMP sequence landscape. Novelty assessment indicated that 1516 GCFs (72.47%) had no similarity to any known AMPs in existing databases. Machine learning predictions suggested that novel AMPs from Lactobacillaceae possessed strong antimicrobial potential, with 664 GCFs having an additive minimum inhibitory concentration (MIC) below 100 μM. We randomly synthesized 16 AMPs (with predicted MIC < 100 μM) and identified 10 AMPs exhibiting varied-spectrum activity against 11 common pathogens. Finally, we identified one Lactobacillus delbrueckii-originated AMP (delbruin_1) having broad-spectrum (all 11 pathogens) and high antimicrobial activity (average MIC = 38.56 µM), which proved its potential as a clinically viable antimicrobial agent.</p><p><strong>Conclusions: </strong>We uncovered the global prevalence of AMPs in Lactobacillaceae and proved that Lactobacillaceae is an untapped and invaluable source of novel AMPs to combat the antibiotic-resistance crisis. Meanwhile, we provided a machine learning-guided framework for AMP discovery, offering a scalable roadmap for identifying novel AMPs not only in Lactobacillaceae but also in other organisms. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"151"},"PeriodicalIF":13.8,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Interactions with native microbial keystone taxa enhance the biocontrol efficiency of Streptomyces.","authors":"Tianyu Sun, Hongwei Liu, Ningqi Wang, Mingcong Huang, Samiran Banerjee, Alexandre Jousset, Yangchun Xu, Qirong Shen, Shimei Wang, Xiaofang Wang, Zhong Wei","doi":"10.1186/s40168-025-02171-1","DOIUrl":"10.1186/s40168-025-02171-1","url":null,"abstract":"","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"148"},"PeriodicalIF":13.8,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12180144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metagenomic analysis reveals gut phage diversity across three mammalian models.","authors":"Menghao Yu, Yunmeng Chu, Yongming Wang, Luofei Mo, Xin Tan, Shun Guo, Shengjian Yuan, Yingfei Ma","doi":"10.1186/s40168-025-02144-4","DOIUrl":"10.1186/s40168-025-02144-4","url":null,"abstract":"<p><strong>Background: </strong>The gut virome plays a pivotal role in shaping the host's microbiota. However, gut viruses across different mammalian models, and their connections with the human gut microbiota remain largely unknown.</p><p><strong>Results: </strong>We identified 977 high-confidence species-level viral operational taxonomic units (vOTUs) in mice (hcMGV), 12,896 in pigs (hcPGV), and 1480 in cynomolgus macaques (hcCMGV) from metagenomes, respectively. Clustering these vOTUs at approximately genus level uncovered novel clades with high prevalence across animal guts (> = 60%). In particular, crAss-like phages and cas-harboring jumbophages were characterized. Comparative analysis revealed that hcCMGV had a closer relationship with hcPGV than hcMGV, despite the animal-specific characteristics, and that 55.88% hcCMGV had connections with the human microbiota.</p><p><strong>Conclusions: </strong>Our findings shed light on the diversity of gut viruses across these three animals, contributing to future gut microbial studies using model animals. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"146"},"PeriodicalIF":13.8,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12180220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Longitudinal host-microbiome dynamics of metatranscription identify hallmarks of progression in periodontitis.","authors":"Ana Duran-Pinedo, Jose O Solbiati, Flavia Teles, Zhang Yanping, Jorge Frias-Lopez","doi":"10.1186/s40168-025-02170-2","DOIUrl":"10.1186/s40168-025-02170-2","url":null,"abstract":"","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"147"},"PeriodicalIF":13.8,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12180239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engrafting gut bacteriophages have potential to modulate microbial metabolism in fecal microbiota transplantation.","authors":"Shuai Ji, Freed Ahmad, Baizhao Peng, Ying Yang, Mengting Su, Xiaoshan Zhao, Tommi Vatanen","doi":"10.1186/s40168-025-02046-5","DOIUrl":"10.1186/s40168-025-02046-5","url":null,"abstract":"<p><strong>Background: </strong>Fecal microbiota transplantation (FMT) is widely used to treat severe infections and investigated for the treatment of complex diseases. The therapeutic efficacy of FMT is related to the successful engraftment of bacteriophages from healthy donors to recipients. However, gut bacteriophage contributions to FMT engraftment and treatment outcomes remain unclear.</p><p><strong>Methods: </strong>The gut phageome from previously published metagenomes of donors and recipients across 23 FMT studies was assembled and functionally annotated for a meta-analysis.</p><p><strong>Results: </strong>Gut phageome profiles of FMT recipients, especially those with recurrent Clostridioides difficile infection (rCDI), shifted toward donor phageomes, accompanied by increased phageome alpha diversity. Engraftment of donor phages varied between recipient conditions with the highest engraftment rate, overrepresented by putative temperate phage, in patients with rCDI. Consistently, a higher proportion of auxiliary metabolic genes (AMGs), with the potential to support and modulate bacterial metabolism, were annotated on putative temperate phages.</p><p><strong>Conclusions: </strong>FMT leads to significant taxonomic, functional, and lifestyle shifts in recipient phageome composition. Future FMT studies should include gut phageome characterization and consider it as a potential factor in microbial community shifts and treatment outcomes. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"149"},"PeriodicalIF":13.8,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12180181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144336740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal intestinal L. vaginalis facilitates embryo implantation and survival through enhancing uterine receptivity in sows.","authors":"Qianhong Ye, Yifan Hu, Haoyi Jiang, Tingting Luo, Longshan Han, Yuwen Chen, Jiaying Chen, Libao Ma, Ziyi He, Xianghua Yan","doi":"10.1186/s40168-025-02141-7","DOIUrl":"10.1186/s40168-025-02141-7","url":null,"abstract":"<p><strong>Background: </strong>The embryo implantation quality during early pregnancy is the predominant factor for embryo survival and litter performance in sows. Gut microbiota is demonstrated to show a correlation to pregnancy outcomes by participating in regulating maternal metabolism. However, the specific functional microbiota and its mechanical effects on regulating embryo implantation and survival remain unclear. The objective of this study was to clarify whether embryo implantation and litter performance were affected by maternal intestinal microbiota, and to identify specific microbial communities and its mechanism in regulating embryo implantation.</p><p><strong>Results: </strong>In this study, we first conducted 16S rRNA sequencing and metabolomic analysis revealing the intestinal microbiota and metabolism of 42 sows with different litter size to select the potential functional microbiota that may contribute to embryo survival. Then, we explored the effects of that microbiota on embryo implantation and litter performance through microbiota transplantation in mice and sows. We found that maternal intestinal L. vaginalis exhibits enrichment in sows with higher litter size, which could facilitate embryo implantation and survival and ultimately increases litter size in mice. We further employed transcriptomic analysis to determine the characteristics of uterus, which found an enhanced uterine receptivity after L. vaginalis gavage. The plasma untargeted metabolomic analysis after L. vaginalis gavage in mice and targeted metabolomics analysis of in vitro cultured medium of L. vaginalis were used to evaluate the metabolic regulation of L. vaginalis and to reveal the underlying functional metabolites. Next, an increasing adhesion rate of endometrial-embryonic cells and an obvious increasing formation of pinopodes in cell surface of porcine endometrial epithelial cells were observed after treatments of L. vaginalis metabolites, especially galangin and daidzein. Also, the gene expression levels related to uterine receptivity were increased after treatments of L. vaginalis metabolites in porcine endometrial epithelial cells. Finally, we found that L. vaginalis or its metabolites supplementation during early gestation significantly increased the litter performance in sows.</p><p><strong>Conclusions: </strong>Overall, intestinal microbial-host interactions can occur during early pregnancy and may be contribute to maternal metabolic changes and influence pregnancy outcomes in mammals. Our study provides insights of maternal intestinal L. vaginalis to enhance uterine receptivity and to benefit embryo/fetal survival through a gut-uterus axis, contributing to advanced concept and novel strategy to manipulate gut microbiota during early pregnancy, and in turn to improve embryo implantation and reduce embryo loss in sows. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"145"},"PeriodicalIF":13.8,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12175382/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of early-life exposures on growth and adult gut microbiome composition is dependent on genetic strain and parent- of- origin.","authors":"M Nazmul Huda, Emer Kelly, Keri Barron, Jing Xue, William Valdar, Lisa M Tarantino, Sarah Schoenrock, Folami Y Ideraabdullah, Brian J Bennett","doi":"10.1186/s40168-025-02130-w","DOIUrl":"10.1186/s40168-025-02130-w","url":null,"abstract":"<p><strong>Background: </strong>Early-life exposure to environmental factors can have long-lasting impacts on offspring health into adulthood and therefore is an emerging public health concern. In particular, the impact of maternal environmental exposures such as diet and antibiotic use on the establishment of the offspring gut microbiome has been recently highlighted as a potential link to disease risk. However, the long-term effects are poorly understood. Moreover, interindividual host genetic differences have also been implicated in modulating the gut microbiome, suggesting that these differences may modulate susceptibility to environmentally induced dysbiosis and exacerbate related health outcomes. Our understanding of how the developmental environment and genetics interact to modulate offspring long-term gut microbiota and health is still limited.</p><p><strong>Methods: </strong>In this study, we investigated the effects of early exposure to known or putative dietary insults on the microbiome (antibiotic exposure, protein deficiency, and vitamin D deficiency) in a novel population of mice. Dams were maintained on purified AIN93G antibiotic-containing (AC), low-protein (LP), low-vitamin D (LVD), or mouse control (CON) diets from 5 weeks prior to pregnancy until the end of lactation. After weaning, mice were transferred to new cages and fed a standardized chow diet. The parent-of-origin (PO) effect was determined via F1 offspring from reciprocal crosses of recombinant inbred intercross (RIX) of Collaborative Cross (CC) mice, where all F1 offspring within a reciprocal pair were genetically identical except for the X- and Y-chromosomes and mitochondrial genomes. We assayed offspring bodyweight and the gut bacterial microbiota via 16S rRNA gene sequencing at 8 weeks of age.</p><p><strong>Results: </strong>Our study revealed that early developmental exposure to antibiotics, protein deficiency, and vitamin D deficiency had long-lasting effects on offspring bodyweight and gut microbial diversity and composition, depending on the genetic background. Several bacterial genera and ASVs, including Bacteroides, Muribaculaceae, Akkermansia, and Bifidobacterium, are influenced by developmental insults. We also observed a significant effect of PO on offspring gut microbiota and growth. For example, the offspring of CC011xCC001 mice had increased bodyweight, microbial diversity indices, and several differential bacterial abundances, including those of Faecalibaculum, compared with those of the corresponding reciprocal cross CC001xCC011.</p><p><strong>Conclusion: </strong>Our results show that maternal exposure to nutritional deficiencies and antibiotics during gestation and lactation has a lasting impact on offspring gut microbiota composition. The specific responses to a diet or antibiotic can vary among F1 strains and may be driven by maternal genetics. Video Abstract.</p>","PeriodicalId":18447,"journal":{"name":"Microbiome","volume":"13 1","pages":"143"},"PeriodicalIF":13.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12168266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}