Medical laboratory sciences最新文献

{"title":"The effects of cholesterol lowering drugs on vitamin D status in familial hypercholesterolemia patients","authors":"Hesam Nasirpour, Yashar Azari Key, Nasrin Kazemipur, B. Shadman, Saba Hajazimian, A. Isazadeh, Sina Taefehshokr","doi":"10.22037/AMLS.V3I4.19099","DOIUrl":"https://doi.org/10.22037/AMLS.V3I4.19099","url":null,"abstract":"Background: Familial high blood cholesterol (hypercholesterolemia) is a common disease that involves many complications for patients. The aim of this study is to investigate the effects of cholesterol-lowering drugs (Gemfibrozil and Atorvastatin) on the level of serum Vitamin D. Materials and methods: In this study, the 25-hydroxy vitamin D levels were evaluated in 65 women between 30-55 years of age. After receiving drug information of patients, cholesterol-lowering medication; Gemfibrozil and Atorvastatin were prescribed by a specialist, then vitamin D and cholesterol levels were measured following 9 month treatment. Also 30 patients consumed vitamin D supplements plus medicine regularly. Results: In the first stage, vitamin D levels in subjects are quite normal but their cholesterol levels were higher than normal. In the second stage, vitamin D levels were measured after 9 months use of Gemfibrozil and Atorvastatin. Accordingly, cholesterol levels decreased significantly due to the use of blood cholesterol-lowering drugs (p=0.021). Also, in this stage the level of vitamin D showed a severe and significant reduction (p=0.041). However, there were no significant reductions in vitamin D in 30 women who consumed vitamin D supplements plus medicine (p=0.073). Conclusion: It seems that taking cholesterol-lowering medicines have reduced the amount of vitamin D. With long-term use of medications, bone diseases such as osteoporosis can be predicted in these individuals. Therefore, taking supplements and food rich in vitamin D during the use of these drugs is recommended.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91522137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of time, temperature and P-chloro-mercuriphenylsulfonic acid during serum storage on HDLᵼ-C and HDL₃-C concentration","authors":"Saeed Pourhassan, N. Maghbouli","doi":"10.22037/AMLS.V3I4.22463","DOIUrl":"https://doi.org/10.22037/AMLS.V3I4.22463","url":null,"abstract":"Background: Accurate measurement of clinical laboratory parameters plays an essential role in the correct interpretation of clinical biochemistry abnormalities. The purpose of this study was evaluation the time and temperature effect on HDLᵼ-C, HDL₂-C and HDL₃-C stability during storage. Material and Methods: 50 adult healthy subjects were participated. For the isolation of HDLᵼ-C, we used precipitation method and HDLᵼ-C data was analyzed by the Abell-Kendal cholesterol reference method. The remaining serum were dispensed into 12 sample tubes and divided into two groups. One of each group was stored upright at room temperature (approximately 25 oC) while another at 4 oC and the half of each group tubes were received p-chloro - mercuriphenylsulfonic acid  (PCMPS). The stored serum aliquots from all temperature and time points were analyzed on 1, 2, 3 days post collection. Results: HDLᵼ-C concentration at the temperature of room in 24 hours is not changed significantly but over the time decreased (7.2% in 3 days). In addition of PCMPS inhibitor, the concentration is increased  by 17.3% in 3 days. But in 4oC, with or without PCMPS, there is no a significant change in the HDLᵼ-C concentration. HDL₃-C was found to be the most stable lipoprotein studied because of non-significant effect of storage time and temperature on it. Conclusion: The results suggest 4oC as the ideal storage condition for the preservation of human serum samples for HDLᵼ-C assay. Also it is suggested that HDL concentration estimation should be performed in the first 24 hours of samples collection. PCMPS addition didn’t affect HDL subtypes concentration in 4oC.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74770574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Restoration of Morphine-Induced Amnesia by Pediococcus acidilactici, an Indigenous Probiotics of Iran, in Male Wistar Rats","authors":"M. Mohammadi","doi":"10.22037/amls.v4i1.22846","DOIUrl":"https://doi.org/10.22037/amls.v4i1.22846","url":null,"abstract":"Background : Probiotics are living microorganisms that have beneficial effects on the microbial balance of the host intestine (human and animal). Studies demonstrated that intestinal microbiota maybe affect the hormones of brain and nervous through the vagus nerve. The goal of this study was to investigate the impact of Lactobacillus Pediococcus acidilactici , indigenous probiotics of Iran isolated from traditional dairy products, on passive avoidance learning of male Wistar rats. Material and Methods : In this research, 80 male Wistar rats weighing from 80 to 100 grams were used. For induction of amnesia, morphine was used as an intraperitoneal injection. Afterward, the mice were with 0.1 ml of milk alone or containing 10 9 CFU/ml of . Pediococcus acidilactici for 8 months. In this study, a non-active avoidance learning behavioral test was used to test long-term memory in Wistar rats. Results : Results displayed that in the control group, morphine (1 mg/rat) significantly decreased learning. In the groups receiving probiotic and probiotic extract plus morphine, there was a significant difference in learning circumstances with the control group. Conclusions : The results of this study depict the beneficial effect of Pediococcus acidilactici , a native Iranian probiotic isolated from dairy products, in the learning quality of non-active conditional avoidance in rats induced by morphine. This indicates the effect of oral probiotics on improving memory and learning.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90151863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the lipid profile in patients with subclinical hypothyroidism","authors":"H. Musavi, M. Abbasi","doi":"10.22037/AMLS.V3I4.22027","DOIUrl":"https://doi.org/10.22037/AMLS.V3I4.22027","url":null,"abstract":"Background : Hypothyroidism is a thyroid gland-related disorder which causes lipid metabolism disturbance. Subclinical Hypothyroidism (SCH) is a compensatory stage in the course of this disease, in which TSH production increases while the levels of thyroid hormones are in normal or low-normal range. There are no studies about the lipid profile abnormalities in SCH. Therefore, the aim of this study was to evaluate the lipid profile in SCH patients and compare it with normal individuals. Methods : In this case-control study, subjects were randomly chosen among  800 individuals referred to Neka city hospital in Mazandaran province, Iran in order to  routine biochemical and thyroid hormone checkup. Participants were divided into two groups ;  cases (n=400) and  controls (n=400). Thyroid hormones were measured by ELISA, and lipid profile parameters were evaluated colorimetrically by AutoAnalyzer. Results : There were no significant differences in age and sex distribution between the two groups. Among the measured thyroid hormones, TSH was significantly different between the two groups (p≤ 0.05). In case of  lipid profile, high-density lipoprotein cholesterol  (HDL-C) was significantly different between the two groups (p≤ 0.05). However, no significant differences were observed in the amount of low-density lipoprotein cholesterol (LDL-C), triglyceride (TG) and cholesterol (p>0.05). Conclusion :  SCH patients, showed a decrease in HDL-C. It can be concluded that subclinical hypothyroidism similar to hypothyroidism can cause lipid metabolism disturbance.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75863153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of autophagy induction and inhibition in the Huh7.5 cell line through flow cytometry","authors":"Mohsen Abdoli, E. Aryan, Hamed Gouklani, Aida Gholoobi, Sajad Naseri, Kiana Ketabi, Z. Meshkat","doi":"10.22037/amls.v3i4.19821","DOIUrl":"https://doi.org/10.22037/amls.v3i4.19821","url":null,"abstract":"Background: Autophagy is a physiologic process in which double membrane vesicles engulf damaged proteins and organelles for delivering them to lysosomein order to degrade and recycle them via lysosomal digestion. Beclin1 is one of the basic proteins involved in the initial step of autophagosome formation. In the current study, the effect of exogenous Beclin1 to induce autophagy and the effect of 3MA to inhibit of autophagy was assessed in Huh7.5 cells as an in vitro models of hepatocellular carcinoma. Material and methods: The Recombinant pcDNA-Beclin1was transfected into Huh7.5 cells. Also, the cell treated with 3MA. Next, the autophagy induction and inhibition was conducted via LC3 staining as a main autophagy marker using flow cytometry. Results: The result of this study suggest that the over expression of exogenous Beclin1 in Huh7.5 cells elevated the autophagosome formation as shown by intracellular autophagosomal marker LC3-II staining for about 32.32 % and   3MA decreased  it up to2% in compared with control cells in which the  stained LC3-II was12.08. Conclusion:  Recombinant beclin1 may be used as a potential autophagy inducer agent and 3-methyl-Adenin inhibits autophagy formation in Huh7.5 cell. The staining autophagy formation marker LC3-II with specific antibody is a reliable method to measure autophagy activation via flow cytometry.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90495715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impurities profiling of seized crystals by GC/MS through 2016 in Iran","authors":"H. Musavi, H. Shokri-Afra, Hemen Moradi-sardareh","doi":"10.22037/AMLS.V3I4.21485","DOIUrl":"https://doi.org/10.22037/AMLS.V3I4.21485","url":null,"abstract":"Introduction Methamphetamine is a highly addictive psychoactive substance which threatens the health of individuals and society. Crystal methamphetamine is an industrial synthetic illegal substance which is made from many chemical reagents. The aim of this study was to analyze methamphetamine samples qualitatively in order to determine the components of this substance. Methods In this study 112 samples of crystalline methamphetamine were analyzed that obtained from Legal Medicine Organization of Iran during the year 2016. In order to determine the chemical characteristics of in samples, Gas Chromatography-mass Spectrometry (GC-MS) were carried out on the samples. Results The results demonstrated that all samples contained methamphetamine. The most frequently occurring synthesis active ingredients, by-products and adulterants were acetic acid, amphetamine and dimethyl amphetamine. Other ingredients were N-formyl amphetamine, N-formyl methamphetamine, N-acetyl methamphetamine, N-acetyl amphetamine, benzyl amphetamine, 2-6-dimethyl-3,5-diphenyl pyridine, N-ethyl methamphetamine, ketamine, ephedrine and pseudoephedrine. About 70% of samples contained phenmetrazine. Conclusion The chemical composition of crystal varies not only with methamphetamine content, but also in the adulterants. Illicit manufacture of methamphetamine results in the formation of some synthesis by-products. Identifying the composition of illicit amphetamines based on the presence or absence of other pharmaceuticals and by-products is presented in this study. All samples were methamphetamine. The fact that N-Benzyl 2-methylaziridine was the most frequently found impurity in the analyzed samples, indicates that most of the methamphetamine samples seized in Iran have been synthesized from ephedrine and pseudoephedrine as starting material.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77109598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-microbial Resistance Pattern of Uropathogens Isolated from Hospitalized Patients in Imam Khomeini hospital, Tehran, Iran","authors":"S. Jasemi, H. Rahdar, Morteza Karami-Zarandi, S. Mahfouzi, A. Abdollahi, M. Feizabadi","doi":"10.22037/AMLS.V3I4.21815","DOIUrl":"https://doi.org/10.22037/AMLS.V3I4.21815","url":null,"abstract":"Background: Urinary tract infection (UTI) is one of the most common type of bacterial infectious diseases which occurs in all age groups. The aim of this study was to determine anti-microbial resistance pattern of bacterial pathogens causing UTIs in hospitalized patients at the Imam Khomeini Hospital, Tehran, Iran. Material and Methods: Urine samples were collected from 11157 hospitalized patients at different wards of Imam Khomeini Hospital in Tehran, between January 2015 and December 2015.The cultured plates were assessed for significant bacterial growth. Anti-microbial susceptibility test was performed using standard disk diffusion method. Results: Out of the 11157 collected urine samples, significant bacterial growth of 25.38% was observed. The most common cause of UTI was gram-negative bacteria (82.2%). More than 50% of the gram-negative bacteria were resistant to ceftriaxone, ceftazidime and trimethoprim-sulfamethoxazole. Enterococcus spp. (10.1%) was found as the third causative agent of UTIs and the most common gram-positive bacteria. Conclusion: we conclude that the examination of the most common etiological agent of UTIs and their antimicrobial resistance patterns is advantageous and necessary in order to design a guideline for empirical therapy.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"275 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80012313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatoprotective and Anti-oxidant effects of Nepeta Ispahanica Boiss extract on CCL4 induced liver Injury","authors":"I. Pouraboli, Fahim Ebrahimi, M. Mirtadzadini","doi":"10.22037/AMLS.V5I1.28540","DOIUrl":"https://doi.org/10.22037/AMLS.V5I1.28540","url":null,"abstract":"Background: Natural products might be applicable as remedial agents with their roles in oxidative stress regulation and as natural antioxidants.  In this regard, Nepeta ispahanica bioss has been utilized in traditional medicine for several functions. Despite numerous properties of the Nepeta species including their antioxidant properties, Nepeta ispahanica boiss effects against hepatic injury induced by carbon tetrachloride (CCl4) have not been studied. This study aimed to investigate the hepatoprotective effects of Nepeta ispahanica boiss on CCL4 induced acute hepatic injury in an animal model.  Materials and Methods: The experiment used a total of 36 male Wistar rats, that were divided into six groups. Except for the intact control groups, all groups received a single intraperitoneal injection of CCl4 after pre‐treatment period with distilled water, Nepeta ispahanica boiss extracts, or legalon. After 24 hours, rats were anesthetized, sacrificed and blood samples were obtained. Serum level of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TB), urea and plasma levels of total protein and malondialdehyde (MDA) were evaluated. Besides, SOD and CAT enzyme activities and GSH levels were determined.  Histopathological studies also were done in liver tissue samples. Results: After the CCl4 injection, oxidative stress‐mediated necrotic acute liver injuries were observed. Also, serum ALP, AST, and ALT elevated. Hepatic lipid peroxidation and related decrease of endogenous antioxidants and antioxidative enzymes; lipid peroxidation markers, and oxidative stress markers were presented. Nevertheless, histological and biochemical markers of liver injury were reserved by the pre‐treatment with Nepeta ispahanica boiss extracts. Conclusion: The present study confirmed that the administration of Nepeta ispahanica boiss extracts before exposure to CCl4, induced significant hepatoprotective effects. These findings verified that Nepeta ispahanica boiss has favorable properties as an antioxidant and hepatoprotective agent.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"6 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90394679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Study of 25 (OH) Vitamin D Serum Levels in Patients with metabolic syndrome and healthy individuals","authors":"E. Rostami, F. Amiri, Z. Mohammadi, Parisa Khanicheragh, Fahimeh Safizadeh, Fariba Mohammadi Tahroodi, H. Javar, H. Aram, Negar Yavari","doi":"10.22037/AMLS.V5I3.29869","DOIUrl":"https://doi.org/10.22037/AMLS.V5I3.29869","url":null,"abstract":"Background: The incidence of metabolic syndrome has been rising in the Iran population. In parallel, vitamin D deficiency has also been increasing in Iran. This study aims to explore the association of vitamin D serum concentrations with metabolic syndrome and its components in the Iranian population.  Materials and Methods: A case-control study was managed. We enrolled 110 metabolic syndrome patients, according to the National Cholesterol Education Program Adult Treatment Panel III (ATP III) criteria as a case group and 130 healthy individuals as a control group. The serum level of 25-hydroxy vitamin D (25 (OH)D), lipid profile, and fasting blood glucose(FBS) status were determined using a commercially available ELISA method. Enzymatic methods determined total cholesterol (Chol), high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL), and triglyceride (TG) levels.  Results: In case group, the prevalence rate of 25 (OH)D deficiency ( 30 ng/mL) was 19.0%. A non-significant association between Chol level and age was noted (p=0.46, p=0.124). The levels of FBS and TG were significantly higher, and the levels of 25 (OH)D, LDL, and HDL were significantly lower in the case of the group compared to the control group. Conclusion: We found that the serum level of 25 (OH)D in patients with metabolic syndrome is lower than in the healthy group, and a low level of 25 (OH)D is related to increased risk of metabolic syndrome and its components.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"94 1","pages":"11-15"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80332482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the normal CSF values in premature neonates with term babies","authors":"M. Fallahi, seyyed Abbas Hamidi, M. Kazemian","doi":"10.22037/AMLS.V5I2.28080","DOIUrl":"https://doi.org/10.22037/AMLS.V5I2.28080","url":null,"abstract":"Background:  Premature infants are at more risk for meningitis, and lumbar puncture is therefore a necessary part of the sepsis workup. The normal cerebrospinal fluid markers of premature infants have not been well established. This study was conducted to define the normal values of cerebrospinal fluid in these infants. Materials and Methods: In this cross-sectional research, we compared the CSF analysis of premature and term neonates admitted to Mahdieh and Mofid children's hospitals from 2014 to 2017. Results: Of 248 neonates, 44.4% were females and 55.6% were males. Fifty-five percent were term and 45% were preterm. The mean birth weight and gestational age of term and preterm babies were 3409 ± 65 and 1373 ±376 gram (P value<0.001) and 38.46 and 31.66 weeks respectively (P value<0.001). The mean age of preterm and term patients at the time of lumbar puncture was 25.96 and 9.66 days respectively (P value<0.001). Overall, analysis of the CSF, protein, glucose, WBC, PMN, and RBC revealed the normal values of 92.7%, 79.4%, 89.9%, 91.5%, and 35.5% respectively. By comparing CSF parameters of term and premature babies, it was exhibited that protein was the only CSF parameter that was significantly different between two given groups (10.23mg/dl vs. 71.47 mg/dl, P-value<0.001). The percentages of abnormal CSF parameters in the two groups was not significantly different. Conclusion: Our study revealed that the CSF markers of term and preterm neonates are similar; the exception was the protein level so that it was significantly high even in the preterm neonates without meningitis or intraventricular hemorrhage.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"78 1","pages":"22-28"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85020961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}