Medical laboratory sciences最新文献_第9页

Comparison of the normal CSF values in premature neonates with term babies 早产儿与足月儿脑脊液正常值的比较

Medical laboratory sciences Pub Date : 2019-01-01 DOI: 10.22037/AMLS.V5I2.28080

M. Fallahi, seyyed Abbas Hamidi, M. Kazemian

{"title":"Comparison of the normal CSF values in premature neonates with term babies","authors":"M. Fallahi, seyyed Abbas Hamidi, M. Kazemian","doi":"10.22037/AMLS.V5I2.28080","DOIUrl":"https://doi.org/10.22037/AMLS.V5I2.28080","url":null,"abstract":"Background: Premature infants are at more risk for meningitis, and lumbar puncture is therefore a necessary part of the sepsis workup. The normal cerebrospinal fluid markers of premature infants have not been well established. This study was conducted to define the normal values of cerebrospinal fluid in these infants. Materials and Methods: In this cross-sectional research, we compared the CSF analysis of premature and term neonates admitted to Mahdieh and Mofid children's hospitals from 2014 to 2017. Results: Of 248 neonates, 44.4% were females and 55.6% were males. Fifty-five percent were term and 45% were preterm. The mean birth weight and gestational age of term and preterm babies were 3409 ± 65 and 1373 ±376 gram (P value<0.001) and 38.46 and 31.66 weeks respectively (P value<0.001). The mean age of preterm and term patients at the time of lumbar puncture was 25.96 and 9.66 days respectively (P value<0.001). Overall, analysis of the CSF, protein, glucose, WBC, PMN, and RBC revealed the normal values of 92.7%, 79.4%, 89.9%, 91.5%, and 35.5% respectively. By comparing CSF parameters of term and premature babies, it was exhibited that protein was the only CSF parameter that was significantly different between two given groups (10.23mg/dl vs. 71.47 mg/dl, P-value<0.001). The percentages of abnormal CSF parameters in the two groups was not significantly different. Conclusion: Our study revealed that the CSF markers of term and preterm neonates are similar; the exception was the protein level so that it was significantly high even in the preterm neonates without meningitis or intraventricular hemorrhage.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"78 1","pages":"22-28"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85020961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the antibacterial potential of Aloe vera as a source of antibacterial agents 芦荟作为抗菌剂来源的抗菌潜力评估

Medical laboratory sciences Pub Date : 2019-01-01 DOI: 10.22037/AMLS.V5I3.30773

A. Niroomand

{"title":"Assessment of the antibacterial potential of Aloe vera as a source of antibacterial agents","authors":"A. Niroomand","doi":"10.22037/AMLS.V5I3.30773","DOIUrl":"https://doi.org/10.22037/AMLS.V5I3.30773","url":null,"abstract":"Background: Nowadays the use of herbs as an alternative to the chemical drug is considered by researchers. Aloe vera belongs to the Asphodelaceae family, a medicinal plant that has been used since ancient times for different pharmaceutical products. This study aimed to evaluate the antibacterial properties of Aloe vera grown in Khouzestan, southwest of Iran. Materials and Methods:For this purpose, ethanol extract was prepared from aerial parts of Aloe vera and its activity was tested against some gram-positive (Enterococcus faecalis, Staphylococcus aureus, Bacillus cereus) and gram-negative (Pseudomonass aeruginosa, Salmonella Typhi, Proteus mirabilis) bacterial species through standard Kirby-Bauer disc diffusion method. Minimum inhibitory concentration and minimum bactericidal concentration were also investigated. Results: The Aloe vera extract showed antibacterial activity against the majority of bacteria. The highest activity (about 25mm inhibition zone) happened against P. aeruginosa but it did not show any inhibitory activity against S.aureus and P. mirabilis. The MIC was found as 10 mg/ml while MBC ≥ 80. Conclusion: Based on the results of this study it can be suggested that Aloe vera contains active antibacterial substances that can be used efficiently for bacterial pathogen control and it should be considered as a potent antimicrobial source for finding new antibiotics, especially against resistant species.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"4 1","pages":"21-25"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88230943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the Sylimarine Effect on IL-10 and IL-17 Production in Blood Samples of Chronic HCV Infected patients Sylimarine对慢性HCV感染者血液样本中IL-10和IL-17产生影响的评价

Medical laboratory sciences Pub Date : 2019-01-01 DOI: 10.22037/AMLS.V5I2.29771

pedram Attaredelgosha

{"title":"Evaluation of the Sylimarine Effect on IL-10 and IL-17 Production in Blood Samples of Chronic HCV Infected patients","authors":"pedram Attaredelgosha","doi":"10.22037/AMLS.V5I2.29771","DOIUrl":"https://doi.org/10.22037/AMLS.V5I2.29771","url":null,"abstract":"Background: The use of herbal remedies, either in combination with conventional drugs or as an alternative, is accepted worldwide. Silymarin derived from Milk thistle has evidence-based therapeutic potency for wide spectrum of liver diseases. The current work aimed to study the immune-modulating activity of Silymarin in HCV-infected patients by measuring the effect of pure Silymarin solution on the production of IL-17 and IL-10. Materials and Methods: Nine HCV-1a infected patients and three healthy controls were entered in this study. The mean age of patients and healthy controls were 45.53 (± 10.21) and 39.9 (±10.88), respectively. The PBMCs were isolated, cultured in 96-well plate and incubated with Silymarin solution (5µg/ml) for 24 hours. The cells and cell culture supernatant of three groups including patients treated with Silymarin, non-treated patients, and healthy controls were then subjected to Real Time PCR and ELISA to measure the levels of inflammatory and non-inflammatory cytokines including IL-17 and IL-10, respectively. Statistical analysis was conducted using SPSS software version 20.0. Results: According to Real Time PCR and ELISA results, the level of IL-17 was significantly reduced in patients treated with Silymarin while the expression of IL-10 was remarkably increased in these patients. Conclusion: The results of this study approved the immunomodulatory properties of Silymarin in HCV-infected patients. Hepatoprotective, antiviral, as well as immunomodulatory properties of the Silymarin make it a potential therapeutic option in patients with chronic hepatitis C.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"21 1","pages":"10-14"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85278450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Serum anti-hsp27 antibodies concentration in diabetes mellitus; population based case-control study 糖尿病患者血清抗hsp27抗体的研究基于人群的病例对照研究

Medical laboratory sciences Pub Date : 2019-01-01 DOI: 10.22037/AMLS.V5I1.30140

M. Tavana, N. Varasteh, Mohadeseh Rohban, H. Ghazizadeh, M. Sadeghzade, S. Tavalaie, Samaneh Abolbashari, H. Ramshini, Z. Meshkat, G. Ferns, M. Mobarhan, F. Sadabadi

{"title":"Serum anti-hsp27 antibodies concentration in diabetes mellitus; population based case-control study","authors":"M. Tavana, N. Varasteh, Mohadeseh Rohban, H. Ghazizadeh, M. Sadeghzade, S. Tavalaie, Samaneh Abolbashari, H. Ramshini, Z. Meshkat, G. Ferns, M. Mobarhan, F. Sadabadi","doi":"10.22037/AMLS.V5I1.30140","DOIUrl":"https://doi.org/10.22037/AMLS.V5I1.30140","url":null,"abstract":"Background: Diabetes mellitus is an important risk factor for cardiovascular disease. Different biomarkers have been investigated for the diagnosis of diabetes pathogenesis or its complications. There are also reports regarding an increased level of anti-HSP27 antibodies in atherogenesis. We aimed to evaluate serum anti-heat shock protein 27 antibodies level in subjects with diabetes mellitus and undiagnosed individuals. Materials and Methods: This cross-sectional study was conducted on 6447 MASHAD study subjects, including four groups with diabetes mellitus (n=610), undiagnosed diabetes (n=162), impaired fasting glucose (IFG) (n=619) and normal (n=5056) subjects. Demographic and anthropometric data were obtained from all participants. Fasting serum glucose (FSG) and other parameters were measured. In-house enzyme-linked immune sorbent assay method was used for measuring Anti-HSP27 antibodies levels. Results: There were significant differences in weight (p=0.034), body mass index, waist, and hip circumference, systolic and diastolic blood pressure, fasting serum glucose, lipid profile and high sensitive- C reactive protein (p<0.001) between four groups of diabetes mellitus, undiagnosed diabetes, impaired fasting glucose, and normal subjects. The serum anti-HSP27 antibody titer did not show a significant difference between studied groups. Conclusion: Serum antibody titers to HSP27 were not significantly different between four groups categorized based on their FSG levels in a large population.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"60 1","pages":"12-17"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90153402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluation of PCR for the Identification of Mycoplasma spp. and Mycoplasma genitalium congenital infection in cord blood 脐带血支原体和生殖道支原体先天性感染的PCR鉴定评价

Medical laboratory sciences Pub Date : 2019-01-01 DOI: 10.22037/AMLS.V5I2.30110

Sakineh Monzavi, M. Shahhosseiny, Mahsa Malekmohammadi Kalahroudi

{"title":"Evaluation of PCR for the Identification of Mycoplasma spp. and Mycoplasma genitalium congenital infection in cord blood","authors":"Sakineh Monzavi, M. Shahhosseiny, Mahsa Malekmohammadi Kalahroudi","doi":"10.22037/AMLS.V5I2.30110","DOIUrl":"https://doi.org/10.22037/AMLS.V5I2.30110","url":null,"abstract":"Background: Congenital infections are the most important threat to human fetal health caused by bacterial, viral, and fungal agents. In this study, PCR diagnostic method has been used on umbilical cord samples to diagnose bacterial agents such as Mycoplasmas, especially Mycoplasma genitalium. Materials and Methods: This study was performed on 100 cord blood samples from cesarean section patients. Limit of detection (LOD) and specificity tests of both PCR was performed. DNA samples were extracted by the DNG plus method and amplified by the PCR technique. The optimized PCR product of 715 bp for Mycoplasma spp. and 427 bp for M. genitalium was amplified and observed on 1.5% gel electrophoresis. DNA specificity testing of seven other organisms revealed a 100% specificity of these primers. The detection limit was set at 100 copy / reaction for Mycoplasma.spp and 1000 copy / reaction for M. genitalium. Results: Of the 100 samples examined, 5 samples were positive for Mycoplasma spp, and no positive cases were observed for M. genitalium. Maternal infections can have important consequences on the fetus. Conclusion: Molecular methods such as PCR could be used for rapid identification of important factors in congenital infections, such as mycoplasma, and provide a good prognosis.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"56 1","pages":"29-35"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74790965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Comparative Study of 25-Hydroxyvitamin D Level and associated Parameters in CKD and Dialysis Patients in Shiraz, Iran 伊朗设拉子地区CKD和透析患者25-羟基维生素D水平及相关参数的比较研究

Medical laboratory sciences Pub Date : 2019-01-01 DOI: 10.22037/AMLS.V5I3.31303

E. Rostami, Zakieh Sadat Sheikhalishahi, Vahideh Behmard, Mohammad Panji, Fatemeh Sadat Moravej, A. Namdari, Sina Dalvand, Azita Aliakbarniya, Negar Yavari, Vajiheh Najafi

{"title":"A Comparative Study of 25-Hydroxyvitamin D Level and associated Parameters in CKD and Dialysis Patients in Shiraz, Iran","authors":"E. Rostami, Zakieh Sadat Sheikhalishahi, Vahideh Behmard, Mohammad Panji, Fatemeh Sadat Moravej, A. Namdari, Sina Dalvand, Azita Aliakbarniya, Negar Yavari, Vajiheh Najafi","doi":"10.22037/AMLS.V5I3.31303","DOIUrl":"https://doi.org/10.22037/AMLS.V5I3.31303","url":null,"abstract":"Background: Vitamin D deficiency and insufficiency generally observed in chronic kidney disease (CKD) and dialysis patients. The goal of this work was to compare the serum levels of vitamin D and its-related metabolic parameters in CKD patients and hemodialysis patients. Materials and Methods: A total of 109 dialysis patients and 99 CKD patients who had not consumed vitamin D for ≥ 1 year were enrolled in this study. 25-Hydroxy vitamin D (25-(OH) D) levels, calcium, alkaline phosphatase (ALP), hemoglobin (Hb), hematocrit (HCT), total iron-binding capacity (TIBC), parathyroid hormone (PTH) and phosphorous were evaluated in the serum of the patients. Results: 25-(OH) D levels in patients with CKD and dialysis patients were 31.73±13.34 ng/mL and 15.52±5.36 ng/mL, respectively. The levels of calcium (P=0.000), ALP (P=0.006), 25(OH) D (P=0.000), FBS (P=0.000), Hb (P=0.002), HCT (P=0.002) and TIBC (P=0.000) were significantly higher, and the levels of PTH (p=0.000) and phosphorous (P=0.000) were significantly lower in the CKD group compared to dialysis group. Conclusion: 25-(OH) D levels and its metabolic parameters except PTH and phosphorus are lower in patients on dialysis compared with the patients with CKD.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"2 1","pages":"16-20"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73257587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Magnetic nanoparticles to improve the contrast of Magnetic Resonance Imaging 磁性纳米颗粒提高磁共振成像的对比度

Medical laboratory sciences Pub Date : 2019-01-01 DOI: 10.22037/AMLS.V5I1.28475

kosar eskandari

{"title":"Magnetic nanoparticles to improve the contrast of Magnetic Resonance Imaging","authors":"kosar eskandari","doi":"10.22037/AMLS.V5I1.28475","DOIUrl":"https://doi.org/10.22037/AMLS.V5I1.28475","url":null,"abstract":"Earlier detection of diseases reduces the mortality rate. So the development of better screening techniques could be considered as a main topic of interest. In non-invasive medical imaging, specifically in Magnetic Resonance Imaging (MRI), conventional contrast agents do not have a good performance in imaging of some fine parts. The prosperity of preclinical researches in oncology that purpose at developing and evaluating curative strategies on samples, requires effective new functionalized contrast agents for portrayal tumor growth, monitoring the trace of a treatment and/or inducing the demolition of cancerous tumors. This study was performed by searching Nanotechnology, Molecular imaging, magnetic nanoparticles, and contrast agent nanoparticles keywords in Google scholar, Science direct, PubMed and Scopus websites in terms of content. We reviewed the recent studies about development of nanoparticles as contrast agents for medical imaging because they have a longer vascular half-life than molecular contrast agents. It could be indicate that nanoparticles are important items in increasing the contrast of the images so that even reducing the size of the magnetic nanoparticles escalates the contrast and half-life of the particles. Particles with a diameter of 10 nm have a greater half-life than particles with a diameter of 30 nm or larger. It was also found that to removing material defects or improve their biocompatibility; particles should be covered with other materials or doped with metals.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88548488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of 8-week resistance training on IRS-1 gene expression in gastrocnemius muscle and glycemic profile in diabetes rats 8周阻力训练对糖尿病大鼠腓肠肌IRS-1基因表达及血糖谱的影响

Medical laboratory sciences Pub Date : 2019-01-01 DOI: 10.22037/AMLS.V5I1.27828

M. Eizadi, Z. Mirakhori, Ashraf Amini

{"title":"The effect of 8-week resistance training on IRS-1 gene expression in gastrocnemius muscle and glycemic profile in diabetes rats","authors":"M. Eizadi, Z. Mirakhori, Ashraf Amini","doi":"10.22037/AMLS.V5I1.27828","DOIUrl":"https://doi.org/10.22037/AMLS.V5I1.27828","url":null,"abstract":"Background: The insulin receptor substrate-1 (IRS-1) has an important role in insulin signaling pathways in the target tissue of obese or insulin-resistant individuals. This study aimed to assess the effect of resistance training on fasting glucose, insulin resistance, and IRS-1 gene expression in gastrocnemius muscle in male Wister rats with type 2 diabetes (T2D). Materials and Methods: For this purpose, T2D induced by high- fat diet (8 weeks) and STZ in fourteen male Wistar rats (220 ± 10 g) and then assigned into exercise (resistance training, 8 weeks, 5 days/weekly, n = 7) and control (no-training, n = 7) by randomly. Fasting blood samples were obtained for measuring glucose, insulin, and calculating insulin resistance (HOMA-IR). Also, the IRS-1 gene expression in gastrocnemius muscle was measured 48 hours after the last training session of both cases and controls. Results: Compared to control, IRS-1 gene expression in gastrocnemius muscle increased significantly by resistance training in exercise groups (p = 0.001). Fasting glucose (p < 0.001) and insulin resistance (p = 0.007) were reduced in the exercise rats compared to the control group. Conclusion: Based on the results, improved fasting glucose and insulin function after resistance training in T2D diabetes could be attributed to enhancing IRS-1 expression in gastrocnemius muscle by training.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"522 1","pages":"23-30"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88962492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Maternal Coagulation disorder on Birth Weight and Post-Natal Non-Coagulation Problems of Neonates 母亲凝血障碍对新生儿出生体重和产后非凝血问题的影响

Medical laboratory sciences Pub Date : 2019-01-01 DOI: 10.22037/AMLS.V5I2.31180

Fatemeh Ekhtesari, Lida Moghadam-Banaem, S. Shahbazi, S. Shahali

{"title":"Effects of Maternal Coagulation disorder on Birth Weight and Post-Natal Non-Coagulation Problems of Neonates","authors":"Fatemeh Ekhtesari, Lida Moghadam-Banaem, S. Shahbazi, S. Shahali","doi":"10.22037/AMLS.V5I2.31180","DOIUrl":"https://doi.org/10.22037/AMLS.V5I2.31180","url":null,"abstract":"Background: To evaluate the birth weight and post-natal non-coagulation problems of infants of mothers with coagulopathies versus normal mothers. Materials and Methods: In a retrospective cohort study, 100 women with coagulation problems attended the Iranian Haemophilia Comprehensive Care Center, and 200 normal women attending two health centers in Tehran and Karaj, with a history of at least one pregnancy were studied. A questionnaire about mothers’ and neonates’ condition was filled out by an interview, and the data were analyzed using SPSS software, version 16. Results: Using linear regression, the maternal coagulation problem had a negative effect on birth weight (p<0.001, β= -0.31). The prevalence of early and prolonged jaundice in newborns of mothers with coagulopathy was higher than that in newborns of normal mothers (12% vs. 2%, and 7% vs. 3%, respectively), the difference between the two groups in terms of incidence of early jaundice was statistically significant (chi square-p<0.001). The frequency of using phototherapy and blood exchange for treating neonatal jaundice, in neonates of mothers with coagulopathy was higher than in neonates of normal mothers (31% vs. 21% and 8% vs. 21%, respectively) (p<0.001). Furthermore, the duration of hospitalization, hospitalization in NICU, and re-hospitalization during the first month of life in neonates of mothers with coagulation problems was higher than in neonates of normal mothers (p<0.001). Conclusion: Considering the high prevalence of neonatal problems in newborns of mothers with coagulation problems and their lower birth weight, further care measures should be provided for them.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"1 1","pages":"36-44"},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83612701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning and prokaryotic expression of the globular head domain of hemagglutinin antigen (HA1) of influenza A (H3N2) virus in Escherichia coli and Bacillus subtilis 甲型流感(H3N2)病毒血凝素抗原(HA1)的克隆及在大肠杆菌和枯草芽孢杆菌中的原核表达

Medical laboratory sciences Pub Date : 2018-10-31 DOI: 10.22037/AMLS.V3I1.17869

Maryam Bidram, F. Behzadian, F. Fotouhi, M. Fazeli

{"title":"Cloning and prokaryotic expression of the globular head domain of hemagglutinin antigen (HA1) of influenza A (H3N2) virus in Escherichia coli and Bacillus subtilis","authors":"Maryam Bidram, F. Behzadian, F. Fotouhi, M. Fazeli","doi":"10.22037/AMLS.V3I1.17869","DOIUrl":"https://doi.org/10.22037/AMLS.V3I1.17869","url":null,"abstract":"Background: The influenza virus hemagglutinin is the major surface protein of the influenza A virus which is composed of HA1 and HA2 subunits. HA1 has an important role in binding of virus to cells and designing neutralizing antibodies. Escherichia coli ( E. coli ) and Bacillus subtilis ( B. subtilis ) both are known as the most useful prokaryotic hosts to express recombinant proteins. The aim of this study was to clone and express recombinant HA1protein in E. coli and B. subtilis bacteria. Materials and Methods: HA1 gene was cloned into pET-28a vector and pHT43 shuttle vector and then, both transformed to E. coli . The recombinant plasmids were extracted and then transformed into the BL21 and WB600 as expressing hosts. After induction with isopropyl-β-d-thiogalactoside (IPTG), expressed recombinant protein was analyzed by SDS-PAGE. Finally, the expressed protein was confirmed by the Western blot. Results: HA1 gene was cloned into pET-28a vector and pHT43 shuttle vector and then, both transformed to E. coli .The recombinant plasmids were extracted and then transformed into the BL21 and WB600 as expressing hosts. After induction with isopropyl-β-d-thiogalactoside (IPTG), expressed recombinant protein was analyzed by SDS-PAGE. Finally, the expressed protein was confirmed by the Western blot. Conclusion: This study demonstrated a strategy for production and purification of recombinant protein in large scale to test as vaccine candidate against influenza and it’s potentially immunogenicity be assessed in animal models.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86907853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0