Medical laboratory sciences最新文献_第7页

Occult Hepatitis B infection (OBI) in vaccinated groups, a metanalysis 接种疫苗组的隐性乙型肝炎感染(OBI)，一项荟萃分析

Medical laboratory sciences Pub Date : 2019-10-30 DOI: 10.22037/AMLS.V1I2.10296

S. Alavian, S. Jazayeri

{"title":"Occult Hepatitis B infection (OBI) in vaccinated groups, a metanalysis","authors":"S. Alavian, S. Jazayeri","doi":"10.22037/AMLS.V1I2.10296","DOIUrl":"https://doi.org/10.22037/AMLS.V1I2.10296","url":null,"abstract":"Nowadays, the presence of HBV DNA in the absence of HBsAg; occult hepatitis B infection; (OBI), is a known clinical entity along with the rapid influx of research being conducted on its clinical relevance. Biologists and clinicians alike have a recent-standing interest in this regards. OBI has been described in several clinical settings. However, the data on its prevalence among immunized and non-immunized healthy general population, in particular, among health care workers (HCWs) is ambigous. This review attemps to explore the significance of OBI in vaccinated groups as a special subject. The prevalence of OBI among general population, vaccinated children/general population and health care workers were: 157 (5.2%), 222 (6.7%) and 33 (1.8%), respectively. The prevalence of anti-HBc among OBI-positive subjects were: 64 (40.7%), 133 (82.7%) and 27 (81.8%), respectively. OBI is partly prevalent in general population and in vaccinated individuals, especially in those who born to HBsAg positive mothers. HBV serological surveys are not enough adequate and sensitive to rule out the presence of HBV DNA. For high-risk groups (subjects born to HBsAg mothers, health care workers, isolated anti-HBc, etc) sensitive molecular tests based on real time PCR should be applied for a proper diagnosis.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"31 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80746275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Relationship between Delivery Type and Jaundice Severity among Newborns Referred to Hospital 转诊新生儿黄疸严重程度与分娩方式的关系

Medical laboratory sciences Pub Date : 2019-10-14 DOI: 10.22037/AMLS.V5I4.32145

Mohammad Panji, Vahideh Behmard, Y. Varghaiyan, Zakieh Sadat Sheikhalishahi, Sajjad Peyvasteh, Effat Seyedhashemi, Mana Zakeri, Farkhonde Pooyanfar, Haleh Barmaki

{"title":"Relationship between Delivery Type and Jaundice Severity among Newborns Referred to Hospital","authors":"Mohammad Panji, Vahideh Behmard, Y. Varghaiyan, Zakieh Sadat Sheikhalishahi, Sajjad Peyvasteh, Effat Seyedhashemi, Mana Zakeri, Farkhonde Pooyanfar, Haleh Barmaki","doi":"10.22037/AMLS.V5I4.32145","DOIUrl":"https://doi.org/10.22037/AMLS.V5I4.32145","url":null,"abstract":"Background: Neonatal jaundice affects one of two newborns around the World and occurs when a baby has a high level of bilirubin in the blood. In recent decades, the cesarean section has increased, accompanied by higher neonatal jaundice risks compared with vaginal delivery. This study aimed to investigate the relationship between the jaundice severity and the delivery type and gender among newborns referred to a Qamar Bani Hashem Hospital in Khoy city (West Azerbaijan Province, Iran). Materials and Methods: In this cross-sectional study, we investigated randomly 309 newborns with asymptomatic jaundice, who referred to the hospital, from 2014 to 2018. The results of laboratory tests were recorded for all participating infants. Results: 49.19% of newborns with jaundice were born by cesarean section, and 49.19% were born by vaginal delivery. 141 (45.63%) of babies with jaundice were males, and 168 (54.37%) were females. Mean blood sugar (BS) (p=0.52), urea (p=0.48), creatinine(Cr) (p=0.69), Na (p=0.46), K (p=0.69), Ca (p=0.29), TB (p=0.58) and neonatal weight (p=0.14) within days 3 to 10 were not significantly different between vaginal delivery group and cesarean section group (p>0.05), while direct bilirubin showed significant variations (p<0.05). Mean serum direct bilirubin was 0.22±0.07 mg/dL in the normal vaginal delivery group and 0.25±0.09 mg/dL in the cesarean section group (p<0.05). Also, the level of K (P=0.04) was significantly higher in the male group compared to the female group. Conclusion: Based on our study, there is no significant relationship between the severity of jaundice and the type of delivery and gender in newborns.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"78 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84085073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Investigating the inhibitory effects of Seidlitzia rosmarinus extract on the amyloid fibril formation of ҡ-casein in the presence of dextran 探讨迷迭香子提取物在葡聚糖存在下对ҡ-casein淀粉样蛋白纤维形成的抑制作用

Medical laboratory sciences Pub Date : 2019-10-14 DOI: 10.22037/AMLS.V4I2.25212

A. Ghahghaei, Khatoon Heidari nasab, Fariba Mohammadi Tahroodi, S. Rahimi

{"title":"Investigating the inhibitory effects of Seidlitzia rosmarinus extract on the amyloid fibril formation of ҡ-casein in the presence of dextran","authors":"A. Ghahghaei, Khatoon Heidari nasab, Fariba Mohammadi Tahroodi, S. Rahimi","doi":"10.22037/AMLS.V4I2.25212","DOIUrl":"https://doi.org/10.22037/AMLS.V4I2.25212","url":null,"abstract":"Background: Formation of amyloid fibrils has been associated with different protein aggregation diseases. Many studies indicate that many proteins can be converted in vitro into amyloid structures. Isolated ҡ-casein (ҡ-CN) spontaneously forms amyloid fibrils under physiological conditions, so it is a convenient model for researching generic aspect of fibril formation. Materials and Methods: In this study the effect of aqueous extract of S. rosmarinus on the amyloid formation of ҡ-CN in the presence and absence of crowding agent, dextran, have been examined using Thioﬂavin T binding (ThT) assay, fluorescence spectroscopy, and circular dichroism (CD) spectroscopy. Results: ThT binding assay showed that dextran increased the rate of amyloid fibril formation and S. rosmarinus extract retarded the amyloid fibril formation in κ-CN. In the presence of dextran however, the effect of S. rosmarinus extract on the amyloid formation of ҡ-CN was less than in its absence. Fluorescence spectroscopy results also demonstrated that dextran led to unfolding and increased the exposure hydrophobic area in ҡ-CN. S. rosmarinus extract efficiency decreased the exposure of hydrophobic regions in κ-CN, whereas in the presence of dextran this effect of extract was reduced. CD spectroscopy results exhibited that incubation of κ-CN with S. rosmarinus extract prevented a structural transition to a β-sheet. CD spectroscopy results also indicated that by adding dextran to reduced κ-CN β-sheet structures observed, which indicates structural change. S. rosmarinus extract however, prevented transition to β-sheet structural. Conclusion: In conclusion our finding suggests that S. rosmarinus extract prevents amyloid fibril formation in κ-CN, although this effect decreased in the presence of dextran.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87461967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Diroﬁlaria repens infection of the breast: a case report 人类双丝虫复发性乳房感染:1例报告

Medical laboratory sciences Pub Date : 2019-10-14 DOI: 10.22037/AMLS.V1I1.9403

S. Maraghi, Ali Sameri, Yasser Jeddi

引用次数: 2

Pathogenesis of Acute Lymphoblastic Leukemia 急性淋巴细胞白血病的发病机制

Medical laboratory sciences Pub Date : 2019-10-13 DOI: 10.22037/AMLS.V4I1.23075

Shahin Aghamiri, Farhad Hajializadeh, A. Jafarpour, Shiva Bayat, Soodeh Namjoo, F. Zaker

引用次数: 0

Molecular investigation of CTX-M gene in Extended Spectrum β Lactamases (ESBLs) producing Pseudomonas aeruginosa isolated from Iranian patients with burn wound infection 伊朗烧伤创面感染患者产扩展谱β内酰胺酶(ESBLs)中CTX-M基因的分子研究

Medical laboratory sciences Pub Date : 2019-10-13 DOI: 10.22037/AMLS.V4I1.23078

F. Piri, M. Ebrahimi, K. Amini

{"title":"Molecular investigation of CTX-M gene in Extended Spectrum β Lactamases (ESBLs) producing Pseudomonas aeruginosa isolated from Iranian patients with burn wound infection","authors":"F. Piri, M. Ebrahimi, K. Amini","doi":"10.22037/AMLS.V4I1.23078","DOIUrl":"https://doi.org/10.22037/AMLS.V4I1.23078","url":null,"abstract":"Background: Pseudomonas aeruginosa (P. aeruginosa) is one of the most important causes of infection in burns and intensive care units. Extended-spectrum β-lactamases (ESBLs) production in P. aeruginosa is a major factor in the antibiotic resistance and is thought of as a serious threat to the currently available antibiotic armory. The purpose of this study was to determine the prevalence of CTX-M gene in ESBL-producing P. aeruginosa isolates in burn wound samples. Materials and methods: In this cross-sectional survey, a total of 60 clinical isolates of P. aeruginosa were obtained from patients suffering from burn wound infection referred to major hospitals of Tehran, Iran. After verification by biochemical tests and antimicrobial susceptibility testing, CTX-M gene was identified using PCR method. Results: The results of the molecular analysis of CTX-M gene showed that the prevalence of isolates of P. aeruginosa harboring CTX-M gene was 20% (12/60). Conclusion: The results from this study showed high levels of antibiotic resistance and CTX-M gene among P. aeruginosa isolated samples of burn-wound infections which condition may result in the increased the emergence of multidrug-resistant strains and the failure of therapy This study suggests that detailed data on the CTX-M gene frequency can be useful to achieve the best therapy for infections caused by ESBLs producing P. aeruginosa.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76769174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

To Reduce the Effects of Experimental Aflatoxicosis in Broiler Chicks Using Specific Egg Yolk Immunoglobulin (IgY) 特异性卵黄免疫球蛋白(IgY)降低肉鸡实验性黄曲霉中毒的作用

Medical laboratory sciences Pub Date : 2019-10-13 DOI: 10.22037/AMLS.V4I1.23058

N. Afzali, A. Nakhaei, S. J. H. Vashan, M. Torshizi

{"title":"To Reduce the Effects of Experimental Aflatoxicosis in Broiler Chicks Using Specific Egg Yolk Immunoglobulin (IgY)","authors":"N. Afzali, A. Nakhaei, S. J. H. Vashan, M. Torshizi","doi":"10.22037/AMLS.V4I1.23058","DOIUrl":"https://doi.org/10.22037/AMLS.V4I1.23058","url":null,"abstract":"Background : This experiment was conducted to evaluate the effects of the specific egg yolk immunoglobulin (IgY) on reducing the defects of experimental aflatoxicosis in broilers. Materials and Methods : In a completely randomized design, a total of 128 Ross 308 broiler chicks were used in 4 treatments, 4 replicates and 8 observations (chicks) for 42 days. Treatments were: 1) control; 2) diet containing 1 ppm aflatoxin B1; 3) diet contaminated with 1 ppm aflatoxin B1 + 0.75 % of egg yolk containing IgY; 4) diet contaminated with 1 ppm aflatoxin B1 + 1.5 % of egg yolk containing IgY. Results : The results showed that the use of aflatoxin containing diet significantly increased the feed conversion ratio, serum cholesterol concentration, serum gamma-glutamyl transferase (GGT), and aspartate aminotransferase (AST). Also, experimental aflatoxicosis resulted in reduced feed intake, weight gain, serum total protein and albumin concentrations (p<0.05); as well as the liver histopathologic lesions. IgY containing egg yolk (0.75% and 1.5%) added to the contaminated ration improved feed intake, weight gain and feed conversion ratio compared to treatment 2 (p<0.05). Compared to treatment 2, serum cholesterol concentration decreased while total protein concentration increased in treatments 3 and 4 (p<0.05). Liver tissue was approximately normal with mild effects on hepatocytes and mild cytoplasmic changes in chicks receiving treatments 3 and 4. Conclusion : It can be concluded that specific IgY is effective in reducing the defects of experimental aflatoxicosis as well as improving performance in broilers.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85239710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

marA efflux pump gene expression in Salmonella enteritidis strains treated with Artemisia tournefortiana hydroalcoholic extract and comparison with commercial efflux pump inhibitor, carbonyl cyanide 3-chlorophenylhydrazone (CCCP) tournefortiana水醇提取物处理肠炎沙门氏菌后marA外排泵基因的表达及与商业外排泵抑制剂羰基氰化3-氯苯腙(CCCP)的比较

Medical laboratory sciences Pub Date : 2019-10-13 DOI: 10.22037/AMLS.V4I1.24090

M. Khosravani, M. Dallal, M. Norouzi

{"title":"marA efflux pump gene expression in Salmonella enteritidis strains treated with Artemisia tournefortiana hydroalcoholic extract and comparison with commercial efflux pump inhibitor, carbonyl cyanide 3-chlorophenylhydrazone (CCCP)","authors":"M. Khosravani, M. Dallal, M. Norouzi","doi":"10.22037/AMLS.V4I1.24090","DOIUrl":"https://doi.org/10.22037/AMLS.V4I1.24090","url":null,"abstract":"Background: Salmonella enterica subsp. enterica serovar Enteritidis is a food-borne pathogenic bacterium that has recently become resistant to most quinolone antibiotics. The MarA efflux pump plays a significant role in the development of ciprofloxacin resistance in S. Enteritidis strains. The aim of this study was comparative evaluation of anti-efflux activity of Artemisia tournefortiana extract and commercial efflux inhibitor, carbonyl cyanide 3-chlorophenylhydrazone (CCCP) on marA efflux pump gene expression in S. Enteritidis clinical strains. M aterials and Methods: In this experimental study, Artemisia tournefortiana extract was prepared using maceration method. Subsequently, MarA efflux pump was detected in 20 clinical strains of S. Enteritidis via cartwheel and PCR methods. Finally, after treatment of strains with subMIC concentration of extract and 20 µg/L and CCCP, their anti-efflux activity against MarA efflux pump was studied using Real Time PCR. R es ults: The results of cartwheel and PCR methods indicated that all of ciprofloxacin resistant strains had MarA efflux pump. Subsequently, after treatment of strains with subMIC concentration of extract and CCCP, results show that both component have the ability to inhibit the MarA efflux pump, significantly. C onclusion: Considering the results of MarA efflux inhibition by A. tournefortiana and CCCP, it seems that this plant can be used as a potential source of drug use as a suppository pump inhibitor instead of CCCP.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87234788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The association between intracellular electrolytes and obesity indices 细胞内电解质与肥胖指数的关系

Medical laboratory sciences Pub Date : 2019-07-15 DOI: 10.22037/AMLS.V4I2.26275

R. Ebrahimi, Naghmeh Jannat Alipour, S. Emamgholipour

{"title":"The association between intracellular electrolytes and obesity indices","authors":"R. Ebrahimi, Naghmeh Jannat Alipour, S. Emamgholipour","doi":"10.22037/AMLS.V4I2.26275","DOIUrl":"https://doi.org/10.22037/AMLS.V4I2.26275","url":null,"abstract":"Background: Obesity with a rapid grow in developed and developing countries has a close association with higher disposition to related diseases such as hypertension. Intracellular functions of sodium, potassium, calcium, phosphate, and iron have been an interested subject in obese patients since their dysregulations are linked to a higher risk of hypertension and other metabolic disorders. Materials and Methods: In this study, the circulating levels of sodium, potassium, calcium, phosphate, and iron were determined in the serum of obese patients compared to normal-weight people. Moreover, we examined the correlation of such electrolytes with the well-known indices of obesity such as body mass index (BMI), waist circumference (WC), hip, triglycerides (TG), cholesterol and other characterizations. Results : The mean levels of sodium, potassium, calcium, phosphate, and iron were significantly different (p < 0.05) in obese patients compared to normal-weight subjects. We observed a positive partial correlation between the levels of these electrolytes and obesity indices such as BMI, WC, hip, and cholesterol. Conclusion : Collectively, the present study suggests the positive correlation between obesity and the indices of metabolic disorders such as hypertension and renal failure according to the observed imbalances in the concentration of electrolytes. Moreover, efforts for diet modification may be helpful in the programs aimed at decreasing the burden of obesity and related disorders.","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77221293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A Survey on the association of glycosylated hemoglobin A1C and hyperlipidemia in patients with type 2 diabetes 2型糖尿病患者糖化血红蛋白A1C与高脂血症的相关性研究

Medical laboratory sciences Pub Date : 2019-07-15 DOI: 10.22037/AMLS.V4I1.21919

H. Musavi, Mojdeh Mahdiannasser, A. Shamshirian, S. Azizi

{"title":"A Survey on the association of glycosylated hemoglobin A1C and hyperlipidemia in patients with type 2 diabetes","authors":"H. Musavi, Mojdeh Mahdiannasser, A. Shamshirian, S. Azizi","doi":"10.22037/AMLS.V4I1.21919","DOIUrl":"https://doi.org/10.22037/AMLS.V4I1.21919","url":null,"abstract":"Background : Type 2 diabetes includes a series of disorders characterized by hyperglycemia, which are followed by dysfunction in insulin secretion. In the clinical laboratory, A1C test defines blood glucose levels over a three-month period. The aim of this study is to evaluate the correlation between HbA1c and hyperlipidemia in patients with type 2 diabetes. Materials and Methods : An cross-sectional study was performed on 209 diabetic patients men (n=109) and women (n=100) with a mean age of 57.1 ± 11.1 years. Diagnosis of diabetes was performed according to the WHO criteria. In this study, venous blood (5 ml) was collected from participants after overnight fasting, and HbA1C levels and lipid profiles were determined using enzymatic methods and auto-analyzer device. Risk factor values (TCH/HDL-C) and atherogenic index of plasma (AIP) (logTG/HDL-c), as well as LDL-C/HDL-C ratio, were calculated. Results : HDL-C and TCH / HDL-C were significantly increased in women. On the other hand, LDL-C/HDL-C and HbA1c levels were significantly higher in men. There was no significant difference in the levels of cholesterol, triglyceride, fast blood sugar (FBS), LDL-C, and AIP between women and men. Cholesterol, FBS, LDL-C and LDL-C/HDL-C also showed a significant direct correlation with HbA1c, but no significant correlation was observed between triglyceride, HDL, AIP, and risk ratio with HbA1c. Conclusion : HbA1c may be considered as a biomarker for dyslipidemia screening in patients with type 2 diabetes","PeriodicalId":18401,"journal":{"name":"Medical laboratory sciences","volume":"532 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91550454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0