Mediators of Inflammation最新文献

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Contribution of m5C RNA Modification-Related Genes to Prognosis and Immunotherapy Prediction in Patients with Ovarian Cancer m5C RNA修饰相关基因对卵巢癌患者预后和免疫治疗预测的贡献
3区 医学
Mediators of Inflammation Pub Date : 2023-11-13 DOI: 10.1155/2023/1400267
Yibin Liu, Shouze Liu, Lu Yan, Qianqian Zhang, Wenhua Liu, Xianghua Huang, Shikai Liu
{"title":"Contribution of m5C RNA Modification-Related Genes to Prognosis and Immunotherapy Prediction in Patients with Ovarian Cancer","authors":"Yibin Liu, Shouze Liu, Lu Yan, Qianqian Zhang, Wenhua Liu, Xianghua Huang, Shikai Liu","doi":"10.1155/2023/1400267","DOIUrl":"https://doi.org/10.1155/2023/1400267","url":null,"abstract":"Background. 5-Methylcytosine (m5C) RNA modification is closely implicated in the occurrence of a variety of cancers. Here, we established a novel prognostic signature for ovarian cancer (OC) patients based on m5C RNA modification-related genes and explored the correlation between these genes with the tumor immune microenvironment. Methods. Methylated-RNA immunoprecipitation sequencing helped us to identify candidate genes related to m5C RNA modification at first. Based on TCGA database, we screened the differentially expressed candidate genes related to the prognosis and constructed a prognostic model using LASSO Cox regression analyses. Notably, the accuracy of the model was evaluated by Kaplan–Meier analysis and receiver operator characteristic curves. Independent prognostic risk factors were investigated by Cox proportional hazard model. Furthermore, we also analyzed the biological functions and pathways involved in the signature. Finally, the immune response of the model was visualized in great detail. Results. Totally, 2,493 candidate genes proved to be involved in m5C modification of RNA for OC. We developed a signature with prognostic value consisting of six m5C RNA modification-related genes. Specially, samples have been split into two cohorts with low- and high-risk scores according to the model, in which the low-risk OC patients exhibited dramatically better overall survival time than those with high-risk scores. Besides, not only was this model a prognostic factor independent of other clinical characteristics but it predicted the intensity of the immune response in OC. Significantly, the accuracy and availability of the signature were verified by ICGC database. Conclusions. Our study bridged the gap between m5C RNA modification and the prognosis of OC and was expected to provide an effective breakthrough for immunotherapy in OC patients.","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"53 15","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136281911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Jia-Wei-Kai-Xin-San Treatment Alleviated Mild Cognitive Impairment through Anti-Inflammatory and Antiapoptotic Mechanisms in SAMP8 Mice. 加味开心散治疗通过抗炎和抗凋亡机制减轻SAMP8小鼠轻度认知障碍。
IF 4.6 3区 医学
Mediators of Inflammation Pub Date : 2023-11-02 eCollection Date: 2023-01-01 DOI: 10.1155/2023/7807302
Xiaolu Zhang, Yingxin Sun, Qun Yu, Wenyun Zeng, Yue Zhang, Miao Zeng, Kexin Pang, Yifei Yu, Jiali Gan, Huhu Li, Lin Yang, Xijuan Jiang
{"title":"Jia-Wei-Kai-Xin-San Treatment Alleviated Mild Cognitive Impairment through Anti-Inflammatory and Antiapoptotic Mechanisms in SAMP8 Mice.","authors":"Xiaolu Zhang, Yingxin Sun, Qun Yu, Wenyun Zeng, Yue Zhang, Miao Zeng, Kexin Pang, Yifei Yu, Jiali Gan, Huhu Li, Lin Yang, Xijuan Jiang","doi":"10.1155/2023/7807302","DOIUrl":"10.1155/2023/7807302","url":null,"abstract":"<p><strong>Background: </strong>Alleviating mild cognitive impairment (MCI) is crucial to delay the progression of Alzheimer's disease (AD). Jia-Wei-Kai-Xin-San (JWKXS) is applied for treating AD with MCI. However, the mechanism of JWKXS in the treatment of MCI is unclear. Thus, this study aimed to investigate the effect and mechanism of JWKXS in SAMP8 mice models of MCI.</p><p><strong>Methods: </strong>MCI models were established to examine learning and memory ability and explore the pathomechanisms in brain of SAMP8 mice at 4, 6, and 8 months. The mice were treated for 8 weeks and the effects of JWKXS on MCI were characterized through Morris water maze and HE/Nissl's/immunohistochemical staining. Its mechanism was predicted by the combination of UPLC-Q-TOF/MS and system pharmacology analysis, further verified with SAMP8 mice, BV2 microglial cells, and PC12 cells.</p><p><strong>Results: </strong>It was found that 4-month-old SAMP8 mice exhibited MCI. Two months of JWKXS treatment improved the learning and memory ability, alleviated the hippocampal tissue and neuron damage. Through network pharmacology, four key signaling pathways were found to be involved in treatment of MCI by JWKXS, including TLR4/NF-<i>κ</i>B pathway, NLRP3 inflammasome activation, and intrinsic and extrinsic apoptosis. <i>In vitro</i> and <i>in vivo</i> experiments demonstrated that JWKXS attenuated neuroinflammation by inhibiting microglia activation, suppressing TLR4/NF-<i>κ</i>B and NLRP3 inflammasome pathways, and blocking the extrinsic and intrinsic apoptotic pathways leading to neuronal apoptosis suppression in the hippocampus.</p><p><strong>Conclusion: </strong>JWKXS treatment improved the learning and memory ability and conferred neuroprotective effects against MCI by inducing anti-inflammation and antiapoptosis. <i>Limitations</i>. The small sample size and short duration of the intervention limit in-depth investigation of the mechanisms. <i>Future Prospects</i>. This provides a direction for further clarification of the anti-AD mechanism, and provides certain data support for the formulation to move toward clinical practice.</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2023 ","pages":"7807302"},"PeriodicalIF":4.6,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10635749/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89718864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
RhoA Promotes Synovial Proliferation and Bone Erosion in Rheumatoid Arthritis through Wnt/PCP Pathway RhoA通过Wnt/PCP通路促进类风湿关节炎滑膜增生和骨侵蚀
3区 医学
Mediators of Inflammation Pub Date : 2023-11-01 DOI: 10.1155/2023/5057009
Ning Chen, Chao-Yue Diao, Xin Huang, Wei-Xing Tan, Ya-Bing Chen, Xin-Yu Qian, Jie Gao, Dong-Bao Zhao
{"title":"RhoA Promotes Synovial Proliferation and Bone Erosion in Rheumatoid Arthritis through Wnt/PCP Pathway","authors":"Ning Chen, Chao-Yue Diao, Xin Huang, Wei-Xing Tan, Ya-Bing Chen, Xin-Yu Qian, Jie Gao, Dong-Bao Zhao","doi":"10.1155/2023/5057009","DOIUrl":"https://doi.org/10.1155/2023/5057009","url":null,"abstract":"Ras homolog gene family member A (RhoA) plays a major role in the Wnt/planar cell polarity (PCP) pathway, which is significantly activated in patients with rheumatoid arthritis (RA). The function of RhoA in RA synovitis and bone erosion is still elusive. Here, we not only explored the impact of RhoA on the proliferation and invasion of RA fibroblast-like synoviocytes (FLSs) but also elucidated its effect on mouse osteoclast and a mouse model of collagen-induced arthritis (CIA). Results showed that RhoA was overexpressed in RA and CIA synovial tissues. Lentivirus-mediated silencing of RhoA increased apoptosis, attenuated invasion, and dramatically upregulated osteoprotegerin/receptor activator of nuclear factor-κB ligand (OPG/RANKL) ratio in RA-FLSs. Additionally, the silencing of RhoA inhibited mouse osteoclast differentiation in vitro and alleviated synovial hyperplasia and bone erosion in the CIA mouse model. These effects in RA-FLSs and osteoclasts were all regulated by RhoA/Rho-associated protein kinase 2 (ROCK2) and might interact with Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathways.","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"496 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135327076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identifying Macrophage-Related Genes in Ulcerative Colitis Using Weighted Coexpression Network Analysis and Machine Learning 利用加权共表达网络分析和机器学习识别溃疡性结肠炎中巨噬细胞相关基因
3区 医学
Mediators of Inflammation Pub Date : 2023-10-25 DOI: 10.1155/2023/4373840
Shaocheng Hong, Hongqian Wang, Shixin Chan, Jiayi Zhang, Bangjie Chen, Xiaohan Ma, Xi Chen
{"title":"Identifying Macrophage-Related Genes in Ulcerative Colitis Using Weighted Coexpression Network Analysis and Machine Learning","authors":"Shaocheng Hong, Hongqian Wang, Shixin Chan, Jiayi Zhang, Bangjie Chen, Xiaohan Ma, Xi Chen","doi":"10.1155/2023/4373840","DOIUrl":"https://doi.org/10.1155/2023/4373840","url":null,"abstract":"Ulcerative colitis (UC) is an inflammatory bowel disease of unknown cause that typically affects the colon and rectum. Innate intestinal immunity, including macrophages, plays a significant role in the pathological development of UC. Using the CIBERSORT algorithm, we observed elevated levels of 22 types of immune cell infiltrates, as well as increased M1 and decreased M2 macrophages in UC compared to normal colonic mucosa. Weighted gene coexpression network analysis (WGCNA) was used to identify modules associated with macrophages and UC, resulting in the identification of 52 macrophage-related genes (MRGs) that were enriched in macrophages at single-cell resolution. Consensus clustering based on these 52 MRGs divided the integrated UC cohorts into three subtypes. Machine learning algorithms were used to identify ectonucleotide pyrophosphatase/phosphodiesterase 1 (ENPP1), sodium- and chloride-dependent neutral and basic amino acid transporter B(0+) (SLC6A14), and 3-hydroxy-3-methylglutaryl-CoA synthase 2 (HMGCS2) in the training set, and their diagnostic value was validated in independent validation sets. Gene set enrichment analysis (GSEA) and gene set variation analysis (GSVA) revealed the main biological effects, and that interleukin-17 was one of several signaling pathways enriched by the three genes. We also constructed a competitive endogenous RNA (CeRNA) network reflecting a potential posttranscriptional regulatory mechanism. Expression of diagnostic markers was validated in vivo and in biospecimens, and our immunohistochemistry (IHC) results confirmed that HMGCS2 gradually decreased during the transformation of UC to colorectal cancer. In conclusion, ENPP1, SLC6A14, and HMGCS2 are associated with macrophages and the progression of UC pathogenesis and have good diagnostic value for patients with UC.","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"56 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134973888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exploration of Hub Genes and Pathogenetic Pathways in Systemic Lupus Erythematosus Complicated with Early Onset Atherosclerosis 系统性红斑狼疮并发早发性动脉粥样硬化的枢纽基因及发病途径探讨
3区 医学
Mediators of Inflammation Pub Date : 2023-10-21 DOI: 10.1155/2023/4508436
Han Zhang, Yinde Huang, Xin Li, Wenbin Chen, Yu Lun, Jian Zhang
{"title":"Exploration of Hub Genes and Pathogenetic Pathways in Systemic Lupus Erythematosus Complicated with Early Onset Atherosclerosis","authors":"Han Zhang, Yinde Huang, Xin Li, Wenbin Chen, Yu Lun, Jian Zhang","doi":"10.1155/2023/4508436","DOIUrl":"https://doi.org/10.1155/2023/4508436","url":null,"abstract":"Background. Notwithstanding the mounting evidence to suggest that systemic lupus erythematosus (SLE) accelerates the progression of atherosclerosis, the mechanisms underlying this phenomenon are yet to be completely understood. This research examined the molecular mechanism behind this vascular complication. Methods. The Gene Expression Omnibus database was retrieved to acquire the gene expression datasets for SLE (GSE109248) and atherosclerosis (GSE100927). The shared differentially expressed genes (DEGs) of SLE and atherosclerosis were screened with the help of the “limma” package in R software, followed by function enrichment analysis, protein–protein interaction (PPI) network construction, key module analysis, hub gene selection, and coexpression analysis. Results. In GSE109248 and GSE100927, 1195 and 418 DEGs in totals were identified, respectively. Subsequently, we acquired 78 common DEGs (70 upregulated genes and eight downregulated genes) with the same expression trends by using the Venn diagram. Finally, 12 hub genes, including PTPRC, TYROBP, FCGR3A, ITGAX, LCP2, IL1B, IRF8, LILRB2, CD68, C1QB, CCR7, and C1QA were identified by using seven different algorithms in Cytohubba. The functional analysis illustrates that these genes were predominantly enriched in immune and inflammation response, lipid and atherosclerosis, and osteoporosis. These results indicate an important role of SLE in inducing excessive inflammation, which may be medicate by these hub genes and can induce osteoporosis and imbalance of the normal mineral balance in the body as well as lipid abnormalities, which eventually leads to premature onset of atherosclerosis. In total, nine transcription factors (TFs) that may participate in regulating the function of these genes were identified. All hub genes and four TFs were validated successfully. Conclusion. The results of our research show that SLE and atherosclerosis have common DEGs, pathophysiology, and hub genes. These findings can provide fresh evidence and insights into a further investigation into the mechanisms at play.","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"24 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135511393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
IgE Immune Complexes Mitigate Eosinophilic Immune Responses through NLRC4 Inflammasome. IgE免疫复合物通过NLRC4炎症小体减轻嗜酸性粒细胞免疫反应。
IF 4.6 3区 医学
Mediators of Inflammation Pub Date : 2023-10-18 eCollection Date: 2023-01-01 DOI: 10.1155/2023/3224708
Ece Oylumlu, Goksu Uzel, Lubeyne Durmus, Ceren Ciraci
{"title":"IgE Immune Complexes Mitigate Eosinophilic Immune Responses through NLRC4 Inflammasome.","authors":"Ece Oylumlu,&nbsp;Goksu Uzel,&nbsp;Lubeyne Durmus,&nbsp;Ceren Ciraci","doi":"10.1155/2023/3224708","DOIUrl":"10.1155/2023/3224708","url":null,"abstract":"<p><p>Immune complexes (ICs) skew immune responses toward either a pro- or anti-inflammatory direction based on the type of stimulation. Immunoglobulin E (IgE) is associated with Th2 immune responses and known to activate innate immune cells. However, roles of antigen (Ag)-specific-IgE ICs in regulating human eosinophil responses remain elusive; therefore, this study builts upon the mechanism of which ovalbumin (Ova)-IgE ICs affects eosinophilic responses utilizing human EoL-1 cell line as a model. Eosinophils are granulocytes functioning through pattern recognition receptors (PRRs) and destructive granule contents in allergic inflammation and parasitic infections. One of the PRRs that eosinophils express is NLRC4, a member of the CARD domain containing nucleotide-binding oligomerization (NOD)-like receptor (NLR) family. Upon recognition of its specific ligand flagellin, NLRC4 inflammasome is formed and leads to the release of interleukin-1<i>β</i> (IL-1<i>β</i>). We exhibited that Ova-IgE ICs induced the NLRC4-inflammasome components, including NLRC4, caspase-1, intracellular IL-1<i>β</i>, and secretion of IL-1<i>β</i>, as well as the granule contents MMP9, TIMP1, and TIMP2 proteins via TLR2 signaling; these responses were suppressed, when NLRC4 inflammasome got actived in the presence of ICs. Furthermore, Ova-IgE ICs induced mRNA expressions of <i>MMP9</i>, <i>TIMP2</i>, and <i>ECP</i> and protein expressions of MMP9 and TIMP2 in EoL-1 through Fc<i>ɛ</i>RII. Interestingly, TLR2 ligand and Ova-IgE ICs costimulation elevated the number of CD63+ cells, a degranulation marker, as compared to the native IgE. Collectively, our findings provide a mechanism for the impacts of Ova-IgE ICs on eosinophilic responses via NLRC4-inflammasome and may help understand eosinophil-associated diseases, including chronic eosinophilic pneumonia, eosinophilic esophagitis, eosinophilic granulomatosis, parasitic infections, allergy, and asthma.</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2023 ","pages":"3224708"},"PeriodicalIF":4.6,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10599938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54229912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Amphiregulin Induces iNOS and COX-2 Expression through NF-κB and MAPK Signaling in Hepatic Inflammation. 肝炎症中双调节蛋白通过NF-κB和MAPK信号传导诱导iNOS和COX-2表达。
IF 4.6 3区 医学
Mediators of Inflammation Pub Date : 2023-10-11 eCollection Date: 2023-01-01 DOI: 10.1155/2023/2364121
Yu Jung Heo, Nami Lee, Sung-E Choi, Ja Young Jeon, Seung Jin Han, Dae Jung Kim, Yup Kang, Kwan Woo Lee, Hae Jin Kim
{"title":"Amphiregulin Induces iNOS and COX-2 Expression through NF-<i>κ</i>B and MAPK Signaling in Hepatic Inflammation.","authors":"Yu Jung Heo,&nbsp;Nami Lee,&nbsp;Sung-E Choi,&nbsp;Ja Young Jeon,&nbsp;Seung Jin Han,&nbsp;Dae Jung Kim,&nbsp;Yup Kang,&nbsp;Kwan Woo Lee,&nbsp;Hae Jin Kim","doi":"10.1155/2023/2364121","DOIUrl":"10.1155/2023/2364121","url":null,"abstract":"<p><strong>Background: </strong>Inflammation is a major cause of hepatic tissue damage and accelerates the progression of nonalcoholic fatty liver disease (NAFLD). Amphiregulin (AREG), an epidermal growth factor receptor ligand, is associated with human liver cirrhosis and hepatocellular carcinoma. We aimed to investigate the effects of AREG on hepatic inflammation during NAFLD progression, <i>in vivo</i> and <i>in vitro</i>.</p><p><strong>Methods: </strong>AREG gene expression was measured in the liver of mice fed a methionine choline-deficient (MCD) diet for 2 weeks. We evaluated inflammatory mediators and signaling pathways in HepG2 cells after stimulation with AREG. Nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) were analyzed using an enzyme-linked immunosorbent assay and western blotting. Nuclear transcription factor kappa-B (NF-<i>κ</i>B) and mitogen-activated protein kinases (MAPKs), including extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 mitogen-activated protein kinase, were analyzed using western blotting.</p><p><strong>Results: </strong>Proinflammatory cytokines (interleukin (IL)-6, IL-1<i>β</i>, and IL-8) and immune cell recruitment (as indicated by L3T4, F4/80, and ly6G mRNA expression) increased, and expression of AREG increased in the liver of mice fed the MCD diet. AREG significantly increased the expression of IL-6 and IL-1<i>β</i> and the production of NO, PGE2, and IL-8 in HepG2 cells. It also activated the protein expression of iNOS and COX-2. AREG-activated NF-<i>κ</i>B and MAPKs signaling, and together with NF-<i>κ</i>B and MAPKs inhibitors, AREG significantly reduced the protein expression of iNOS and COX-2.</p><p><strong>Conclusion: </strong>AREG plays a role in hepatic inflammation by increasing iNOS and COX-2 expression via NF-<i>κ</i>B and MAPKs signaling.</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2023 ","pages":"2364121"},"PeriodicalIF":4.6,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10586434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49691349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dimethyl Fumarate Protects against Lipopolysaccharide- (LPS-) Induced Sepsis through Inhibition of NF-κB Pathway in Mice. 富马酸二甲酯通过抑制小鼠NF-κB通路保护小鼠免受脂多糖(LPS)诱导的败血症。
IF 4.4 3区 医学
Mediators of Inflammation Pub Date : 2023-10-05 eCollection Date: 2023-01-01 DOI: 10.1155/2023/5133505
He Fang, Xingtong Wang, Mahendra Damarla, Rongju Sun, Qingli He, Ruojing Li, Pengfei Luo, Jun O Liu, Zhaofan Xia
{"title":"Dimethyl Fumarate Protects against Lipopolysaccharide- (LPS-) Induced Sepsis through Inhibition of NF-<i>κ</i>B Pathway in Mice.","authors":"He Fang, Xingtong Wang, Mahendra Damarla, Rongju Sun, Qingli He, Ruojing Li, Pengfei Luo, Jun O Liu, Zhaofan Xia","doi":"10.1155/2023/5133505","DOIUrl":"10.1155/2023/5133505","url":null,"abstract":"<p><p>Sepsis is one of the most severe complications and causes of mortality in the clinic. It remains a great challenge with no effective treatment for clinicians worldwide. Inhibiting the release of proinflammatory cytokines during sepsis is considered as an important strategy for treating sepsis and improving survival. In the present study, we have observed the effect of dimethyl fumarate (DMF) on lipopolysaccharide- (LPS-) induced sepsis and investigated the possible mechanism. By screening a subset of the Johns Hopkins Drug Library, we identified DMF as a novel inhibitor of nitric oxide synthesis in LPS-stimulated RAW264.7 cells, suggesting that DMF could be a potential drug to treat sepsis. To further characterize the effect of DMF on LPS signaling, TNF-<i>α</i>, MCP-1, G-CMF, and IL-6 expression levels were determined by using cytokine array panels. In addition, an endotoxemia model with C57BL/6 mice was used to assess the in vivo efficacy of DMF on sepsis. The survival rate was assessed, and HE staining was performed to investigate histopathological damage to the organs. DMF was found to increase the survival of septic mice by 50% and attenuate organ damage, consistent with the reduction in IL-10, IL-6, and TNF-<i>α</i> (inflammatory cytokines) in serum. In vitro experiments revealed DMF's inhibitory effect on the phosphorylation of p65, I<i>κ</i>B, and IKK, suggesting that the primary inhibitory effects of DMF can be attributed, at least in part, to the inhibition of phosphorylation of I<i>κ</i>B<i>α</i>, IKK as well as nuclear factor-<i>κ</i>B (NF-<i>κ</i>B) upon LPS stimulation. The findings demonstrate that DMF dramatically inhibits NO and proinflammatory cytokine production in response to LPS and improves survival in septic mice, raising the possibility that DMF has the potential to be repurposed as a new treatment of sepsis.</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2023 ","pages":"5133505"},"PeriodicalIF":4.4,"publicationDate":"2023-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10569893/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41236654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Empagliflozin Reverses Oxidized LDL-Induced RECK Suppression, Cardiotrophin-1 Expression, MMP Activation, and Human Aortic Smooth Muscle Cell Proliferation and Migration. 恩帕列嗪逆转氧化低密度脂蛋白诱导的RECK抑制、心肌营养素-1表达、MMP激活以及人主动脉平滑肌细胞增殖和迁移。
IF 4.4 3区 医学
Mediators of Inflammation Pub Date : 2023-10-04 eCollection Date: 2023-01-01 DOI: 10.1155/2023/6112301
Bysani Chandrasekar, Srinivas Mummidi, Vincent G DeMarco, Yusuke Higashi
{"title":"Empagliflozin Reverses Oxidized LDL-Induced RECK Suppression, Cardiotrophin-1 Expression, MMP Activation, and Human Aortic Smooth Muscle Cell Proliferation and Migration.","authors":"Bysani Chandrasekar, Srinivas Mummidi, Vincent G DeMarco, Yusuke Higashi","doi":"10.1155/2023/6112301","DOIUrl":"10.1155/2023/6112301","url":null,"abstract":"<p><p>Persistent oxidative stress and inflammation contribute causally to smooth muscle cell (SMC) proliferation and migration, the characteristic features of vascular proliferative diseases. Oxidatively modified low-density lipoproteins (OxLDL) elevate oxidative stress levels, inflammatory responses, and matrix metallopeptidase (MMP) activation, resulting ultimately in SMC migration, proliferation, and phenotype change. Reversion-inducing cysteine-rich protein with Kazal motifs (RECK) is a membrane-anchored MMP inhibitor. Empagliflozin is an SGLT2 inhibitor and exerts pleiotropic cardiovascular protective effects, including antioxidant and anti-inflammatory effects. Here, we investigated (i) whether OxLDL regulates RECK expression, (ii) whether ectopic expression of RECK reverses OxLDL-induced SMC migration and proliferation, and (iii) whether pretreatment with empagliflozin reverses OxLDL-induced RECK suppression, MMP activation, and SMC migration, proliferation, and differentiation. Indeed, results show that OxLDL at pathophysiological concentration promotes SMC migration and proliferation via NF-<i>κ</i>B/miR-30b-dependent RECK suppression. Moreover, OxLDL changed the SMC phenotype to a more pro-inflammatory type, and this effect is blunted by RECK overexpression. Further, treatment with empagliflozin reversed OxLDL-induced miR-30b induction, RECK suppression, MMP activation, SMC migration, proliferation, and proinflammatory phenotype changes. OxLDL-induced cardiotrophin (CT)-1 expression and CT-1 stimulated SMC proliferation and migration in part via leukemia inhibitory factor receptor (LIFR) and glycoprotein 130 (gp130). Ectopic expression of RECK inhibited these effects by physically associating with LIFR and gp130, as evidenced by immunoprecipitation/immunoblotting and double immunofluorescence. Importantly, empagliflozin inhibited CT-1-induced mitogenic and migratory effects. Together, these results suggest the therapeutic potential of sustaining RECK expression or empagliflozin in vascular diseases characterized by SMC proliferation and migration.</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2023 ","pages":"6112301"},"PeriodicalIF":4.4,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10567511/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41204642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Retracted: Cortical Plasticity Mechanism and Efficacy Prediction of Repeated Transcranial Magnetic Stimulation in the Treatment of Depression with Continuous Short Bursts of Rapid Pulse Stimulation (cTBS). 回缩:重复经颅磁刺激治疗持续短脉冲快速刺激(cTBS)抑郁症的皮层可塑性机制和疗效预测。
IF 4.6 3区 医学
Mediators of Inflammation Pub Date : 2023-10-04 eCollection Date: 2023-01-01 DOI: 10.1155/2023/9804270
Mediators Of Inflammation
{"title":"Retracted: Cortical Plasticity Mechanism and Efficacy Prediction of Repeated Transcranial Magnetic Stimulation in the Treatment of Depression with Continuous Short Bursts of Rapid Pulse Stimulation (cTBS).","authors":"Mediators Of Inflammation","doi":"10.1155/2023/9804270","DOIUrl":"10.1155/2023/9804270","url":null,"abstract":"<p><p>[This retracts the article DOI: 10.1155/2022/5741114.].</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2023 ","pages":"9804270"},"PeriodicalIF":4.6,"publicationDate":"2023-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10567506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41204643","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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