Macrophages Promote Atherosclerosis Development by Inhibiting CD8T Cell Apoptosis.

IF 4.4 3区医学 Q2 CELL BIOLOGY

Mediators of Inflammation Pub Date : 2024-09-21 eCollection Date: 2024-01-01 DOI:10.1155/2024/1929766

Xiaoming Xu, Yuteng Wu, Yifei Xu, Wei Mao, Yanyun Pan

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引用次数: 0

Abstract

Background: Atherosclerosis is an inflammatory cardiovascular disease. However, whether the association of immune cells in plaques promotes the progression of this disease has not yet been completely elucidated.

Materials and methods: Thus, this study aimed to investigate the relationship between C1q+ macrophages and CD8T cells through scRNA-seq data reanalysis, quantitative real-time PCR, and flow cytometry. Chromatin immunoprecipitation-quantitative polymerase chain reaction, western blot, and antibody-blocking experiments were performed to investigate the role of macrophage-CD8T interaction in atherosclerosis. An atherosclerotic mouse model was developed to confirm our findings.

Results: Mechanistically, Spi1 expression induced by granulocyte-macrophage colony-stimulating factor promoted C1q expression in the macrophages. Moreover, C1q+ macrophages suppressed CD8T cell apoptosis by upregulating Slc7a7 expression to enhance the L-arginine uptake of CD8T cells. CD8T-derived interferon-γ promoted macrophage activation to induce atherosclerosis. Blockade of the C1q-C1qbp axis attenuated atherosclerosis.

Conclusion: In conclusion, macrophages interacting with CD8T promote atherosclerosis development via the C1q-C1qbp axis.

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巨噬细胞通过抑制 CD8T 细胞凋亡促进动脉粥样硬化的发展

背景：动脉粥样硬化是一种炎症性心血管疾病：动脉粥样硬化是一种炎症性心血管疾病。然而，斑块中免疫细胞的关联是否会促进这种疾病的进展尚未完全阐明：因此，本研究旨在通过scRNA-seq数据再分析、定量实时PCR和流式细胞术研究C1q+巨噬细胞和CD8T细胞之间的关系。通过染色质免疫沉淀-定量聚合酶链反应、Western 印迹和抗体阻断实验，研究巨噬细胞与 CD8T 相互作用在动脉粥样硬化中的作用。为了证实我们的发现，还建立了动脉粥样硬化小鼠模型：结果：从机理上讲，粒细胞-巨噬细胞集落刺激因子诱导的 Spi1 表达促进了巨噬细胞中 C1q 的表达。此外，C1q+巨噬细胞通过上调Slc7a7的表达来增强CD8T细胞对L-精氨酸的摄取，从而抑制CD8T细胞的凋亡。CD8T 衍生的干扰素-γ促进巨噬细胞活化，诱发动脉粥样硬化。阻断C1q-C1qbp轴可减轻动脉粥样硬化：总之，巨噬细胞与 CD8T 相互作用，通过 C1q-C1qbp 轴促进动脉粥样硬化的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mediators of Inflammation 医学-免疫学

CiteScore

8.70

自引率

0.00%

发文量

202

审稿时长

4 months

期刊介绍： Mediators of Inflammation is a peer-reviewed, Open Access journal that publishes original research and review articles on all types of inflammatory mediators, including cytokines, histamine, bradykinin, prostaglandins, leukotrienes, PAF, biological response modifiers and the family of cell adhesion-promoting molecules.