Mediators of Inflammation最新文献

筛选
英文 中文
Atherosclerosis and Toll-Like Receptor4 (TLR4), Lectin-Like Oxidized Low-Density Lipoprotein-1 (LOX-1), and Proprotein Convertase Subtilisin/Kexin Type9 (PCSK9) 动脉粥样硬化与 Toll-Like Receptor4 (TLR4)、Lectin-Like Oxidized Low-Density Lipoprotein-1 (LOX-1) 和 Proprotein Convertase Subtilisin/Kexin Type9 (PCSK9)
IF 4.6 3区 医学
Mediators of Inflammation Pub Date : 2024-02-27 DOI: 10.1155/2024/5830491
Bahador Bagheri, Zahra Khatibiyan Feyzabadi, Ahmad Nouri, Ali Azadfallah, Mahyar Mahdizade Ari, Maral Hemmati, Mahboubeh Darban, Parisa Alavi Toosi, Seyedeh Zahra Banihashemian
{"title":"Atherosclerosis and Toll-Like Receptor4 (TLR4), Lectin-Like Oxidized Low-Density Lipoprotein-1 (LOX-1), and Proprotein Convertase Subtilisin/Kexin Type9 (PCSK9)","authors":"Bahador Bagheri, Zahra Khatibiyan Feyzabadi, Ahmad Nouri, Ali Azadfallah, Mahyar Mahdizade Ari, Maral Hemmati, Mahboubeh Darban, Parisa Alavi Toosi, Seyedeh Zahra Banihashemian","doi":"10.1155/2024/5830491","DOIUrl":"https://doi.org/10.1155/2024/5830491","url":null,"abstract":"Atherosclerosis is a leading cause of death in the world. A significant body of evidence suggests that inflammation and various players are implicated and have pivotal roles in the formation of atherosclerotic plaques. Toll-like receptor 4 (TLR4) is linked with different stages of atherosclerosis. This receptor is highly expressed in the endothelial cells (ECs) and atherosclerotic plaques. TLR4 activation can lead to the production of inflammatory cytokines and related responses. Lectin-like oxidized low-density lipoprotein-1 (LOX-1), an integral membrane glycoprotein with widespread expression on the ECs, is involved in atherosclerosis and has some common pathways with TLR4 in atherosclerotic lesions. In addition, proprotein convertase subtilisin/kexin type9 (PCSK9), which is a regulatory enzyme with different roles in cholesterol uptake, is implicated in atherosclerosis. At present, TLR4, PCSK9, and LOX-1 are increasingly acknowledged as key players in the pathogenesis of atherosclerotic cardiovascular diseases. Herein, we presented the current evidence on the structure, functions, and roles of TLR4, PCSK9, and LOX-1 in atherosclerosis.","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"1 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139979201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Protective Role of Canonical Wnt/β-Catenin Pathway in Pathogenic Bacteria-Induced Inflammatory Responses Canonical Wnt/β-Catenin 通路在致病细菌诱发的炎症反应中的保护作用
IF 4.6 3区 医学
Mediators of Inflammation Pub Date : 2024-02-27 DOI: 10.1155/2024/8869510
Zhongjia Jiang, Weiping Zhou, Xing Tian, Peng Zou, Ning Li, Chunmeng Zhang, Yanting Li, Guangyan Liu
{"title":"A Protective Role of Canonical Wnt/β-Catenin Pathway in Pathogenic Bacteria-Induced Inflammatory Responses","authors":"Zhongjia Jiang, Weiping Zhou, Xing Tian, Peng Zou, Ning Li, Chunmeng Zhang, Yanting Li, Guangyan Liu","doi":"10.1155/2024/8869510","DOIUrl":"https://doi.org/10.1155/2024/8869510","url":null,"abstract":"Inflammation is a complex host defensive response against various disease-associated pathogens. A baseline extent of inflammation is supposed to be tightly associated with a sequence of immune-modulated processes, resulting in the protection of the host organism against pathogen invasion; however, as a matter of fact is that an uncontrolled inflammatory cascade is the main factor responsible for the host damage, accordingly suggesting a significant and indispensable involvement of negative feedback mechanism in modulation of inflammation. Evidence accumulated so far has supported a repressive effect of the canonical Wnt/<i>β</i>-catenin pathway on microbial-triggered inflammation via diverse mechanisms, although that consequence is dependent on the cellular context, types of stimuli, and cytokine environment. It is of particular interest and importance to comprehend the precise way in which the Wnt/<i>β</i>-catenin pathway is activated, due to its essential anti-inflammatory properties. It is assumed that an inflammatory milieu is necessary for initiating and activating this signaling, implying that Wnt activity is responsible for shielding tissues from overwhelming inflammation, thus sustaining a balanced physiological condition against bacterial infection. This review gathers the recent efforts to elucidate the mechanistic details through how Wnt/<i>β</i>-catenin signaling modulates anti-inflammatory responses in response to bacterial infection and its interactions with other inflammatory signals, which warrants further study for the development of specific interventions for the treatment of inflammatory diseases. Further clinical trials from different disease settings are required.","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"23 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139979326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
STEAP3 Affects Ovarian Cancer Progression by Regulating Ferroptosis through the p53/SLC7A11 Pathway STEAP3 通过 p53/SLC7A11 通路调控铁突变,从而影响卵巢癌的进展
IF 4.6 3区 医学
Mediators of Inflammation Pub Date : 2024-02-26 DOI: 10.1155/2024/4048527
Yi Han, Lei Fu, Yan Kong, Changqing Jiang, Liying Huang, Hualing Zhang
{"title":"STEAP3 Affects Ovarian Cancer Progression by Regulating Ferroptosis through the p53/SLC7A11 Pathway","authors":"Yi Han, Lei Fu, Yan Kong, Changqing Jiang, Liying Huang, Hualing Zhang","doi":"10.1155/2024/4048527","DOIUrl":"https://doi.org/10.1155/2024/4048527","url":null,"abstract":"Ovarian cancer (OC) is a common malignant cancer in women with a low overall survival rate, and ferroptosis may be a potential new strategy for treatment. Six-transmembrane epithelial antigen of prostate 3 (STEAP3) is a gene closely related to ferroptosis, yet the role of STEAP3 in OC has not yet been thoroughly investigated. Using biological information analysis, we first found that STEAP3 was highly expressed in OC, which was significantly associated with poor prognosis of patients and was an independent prognostic factor. Through cloning, scratch, and transwell experiments, we subsequently found that knockdown of STEAP3 significantly reduced the proliferation and migration ability of OC cells. Furthermore, we found that knockdown of STEAP3 induced ferroptosis in OC cells by detecting ferroptosis indicators. Mechanistically, we also found that knockdown of STEAP3 induced ferroptosis through the p53/SLC7A11 signaling pathway. Through tumorigenic experiments in nude mice, we finally verified that the knockdown of STEAP3 could inhibit tumor growth in vivo by promoting ferroptosis through the p53 pathway. Overall, our study identified a novel therapeutic target for ferroptosis in OC and explored its specific mechanism of action.","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"3 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139968526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Potential Association of the Oral Microbiome with Trimethylamine N-Oxide Quantification in Mexican Patients with Myocardial Infarction 墨西哥心肌梗死患者口腔微生物组与三甲胺氧化物定量的潜在联系
IF 4.6 3区 医学
Mediators of Inflammation Pub Date : 2024-02-20 DOI: 10.1155/2024/3985731
Paulina Hernández-Ruiz, Alma R. Escalona Montaño, Luis M. Amezcua-Guerra, Héctor González-Pacheco, Elena Niccolai, Amedeo Amedei, María M. Aguirre-García
{"title":"Potential Association of the Oral Microbiome with Trimethylamine N-Oxide Quantification in Mexican Patients with Myocardial Infarction","authors":"Paulina Hernández-Ruiz, Alma R. Escalona Montaño, Luis M. Amezcua-Guerra, Héctor González-Pacheco, Elena Niccolai, Amedeo Amedei, María M. Aguirre-García","doi":"10.1155/2024/3985731","DOIUrl":"https://doi.org/10.1155/2024/3985731","url":null,"abstract":"Many attempts have been proposed to evaluate the linkage between the oral–gut–liver axis and the mechanisms related to the diseases’ establishment. One of them is the oral microbiota translocation into the bloodstream, liver, and gut, promoting a host dysbiosis and triggering the presence of some metabolites such as trimethylamine N-oxide (TMAO), known as a risk marker for cardiovascular disease, and especially the myocardial infarction (MI). In the present pilot study, the involvement of oral dysbiosis related to the presence of TMAO has been considered an independent component of the standard risk factors (SRs) in the development of MI, which has not been previously described in human cohorts. A positive and significant correlation of TMAO levels with <i>Porphyromonas</i> was identified; likewise, the increase of the genus <i>Peptidiphaga</i> in patients without SRs was observed. We determined that the presence of SRs does not influence the TMAO concentration in these patients. This report is the first study where the relationship between oral dysbiosis and TMAO is specified in the Mexican population. Our findings provide information on the possible contribution of the oral pathogens associated with gut dysbiosis in the development of MI, although further analysis should be performed.","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"194 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139925945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Interference in Macrophage Balance (M1/M2): The Mechanism of Action Responsible for the Anti-Inflammatory Effect of a Fluorophenyl-Substituted Imidazole 干扰巨噬细胞平衡(M1/M2):氟苯基取代咪唑的抗炎作用机制
IF 4.6 3区 医学
Mediators of Inflammation Pub Date : 2024-02-17 DOI: 10.1155/2024/9528976
Julia Salvan da Rosa, Eduarda Talita Bramorski Mohr, Tainá Larissa Lubschinski, Guilherme Nicácio Vieira, Thais Andreia Rossa, Marcus Mandolesi Sá, Eduardo Monguilhott Dalmarco
{"title":"Interference in Macrophage Balance (M1/M2): The Mechanism of Action Responsible for the Anti-Inflammatory Effect of a Fluorophenyl-Substituted Imidazole","authors":"Julia Salvan da Rosa, Eduarda Talita Bramorski Mohr, Tainá Larissa Lubschinski, Guilherme Nicácio Vieira, Thais Andreia Rossa, Marcus Mandolesi Sá, Eduardo Monguilhott Dalmarco","doi":"10.1155/2024/9528976","DOIUrl":"https://doi.org/10.1155/2024/9528976","url":null,"abstract":"Traditionally, the treatment of inflammatory conditions has focused on the inhibition of inflammatory mediator production; however, many conditions are refractory to this classical approach. Recently, an alternative has been presented by researchers to solve this problem: The immunomodulation of cells closely related to inflammation. Hence, macrophages, a critical key in both innate and acquired immunity, have been presented as an alternative target for the development of new medicines. In this work, we tested the fluorophenyl-imidazole for its anti-inflammatory activity and possible immunomodulatory effect on RAW 264.7 macrophages. We also evaluated the anti-inflammatory effect of the compound, and the macrophage repolarization to M2 was confirmed by the ability of the compound to reduce the M1 markers TNF-<i>α</i>, IL-6, MCP-1, IL-12p70, IFN-<i>γ</i>, and TLR4, the high levels of p65 phosphorylated, iNOS and COX-2 mRNA expression, and the fact that the compound was not able to induce the production of M1 markers when used in macrophages without lipopolysaccharide (LPS) stimulation. Moreover, fluorophenyl-imidazole had the ability to increase the M2 markers IL-4, IL-13, CD206, apoptosis and phagocytosis levels, arginase-1, and FIZZ-1 mRNA expression before LPS stimulation. Similarly, it was also able to induce the production of these same M2 markers in macrophages without being induced with LPS. These results reinforce the affirmation that the fluorophenyl-imidazole has an important anti-inflammatory effect and demonstrates that this effect is due to immunomodulatory activity, having the ability to trigger a repolarization of macrophages from M1 to M2a. These facts suggest that this molecule could be used as an alternative scaffold for the development of a new medicine to treat inflammatory conditions, where the anti-inflammatory and proregenerative properties of M2a macrophages are desired.","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"18 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-02-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139767621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Salvianolic Acid B Alleviates High Glucose-Induced Vascular Smooth Muscle Cell Inflammation by Upregulating the miR-486a-5p Expression 丹酚酸 B 通过上调 miR-486a-5p 的表达缓解高血糖诱导的血管平滑肌细胞炎症
IF 4.6 3区 医学
Mediators of Inflammation Pub Date : 2024-02-16 DOI: 10.1155/2024/4121166
Man-Li Zhang, Man-Na Zhang, Hui Chen, Xia Wang, Kun Zhao, Xuan Li, Xuan Song, Fei Tong
{"title":"Salvianolic Acid B Alleviates High Glucose-Induced Vascular Smooth Muscle Cell Inflammation by Upregulating the miR-486a-5p Expression","authors":"Man-Li Zhang, Man-Na Zhang, Hui Chen, Xia Wang, Kun Zhao, Xuan Li, Xuan Song, Fei Tong","doi":"10.1155/2024/4121166","DOIUrl":"https://doi.org/10.1155/2024/4121166","url":null,"abstract":"The macrovascular complications of diabetes cause high mortality and disability in patients with type 2 diabetes mellitus (T2DM). The inflammatory response of vascular smooth muscle cell (VSMC) runs through its pathophysiological process. Salvianolic acid B (Sal B) exhibits beneficial effects on the cardiovascular system. However, its role and mechanism in diabetic vascular inflammatory response remain unclear. In this study, we found that Sal B reduced vascular inflammation in diabetic mice and high glucose- (HG-) induced VSMC inflammation. Subsequently, we found that Sal B reduced HG-induced VSMC inflammation by downregulating FOXO1. Furthermore, miR-486a-5p expression was obviously reduced in HG-treated VSMC. Sal B attenuated HG-induced VSMC inflammation by upregulating miR-486a-5p. Loss- and gain-of-function experiments had proven that the transfection of the miR-486a-5p mimic inhibited HG-induced VSMC inflammation whereas that of the miR-486a-5p inhibitor promoted HG-induced VSMC inflammation, thereby leading to the amelioration of vascular inflammation in the diabetic mice. Furthermore, studies had shown that miR-486a-5p inhibited FOXO1 expression by directly targeting its 3<sup>′</sup>-UTR. In conclusion, Sal B alleviates the inflammatory response of VSMC by upregulating miR-486a-5p and aggravating its inhibition of FOXO1 expression. Sal B exerts a significant anti-inflammatory effect in HG-induced VSMC inflammation by modulating the miR-486a-5p/FOXO1 axis.","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"215 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139767614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification and Verification of Novel Biomarkers Involving Rheumatoid Arthritis with Multimachine Learning Algorithms: An In Silicon and In Vivo Study 利用多机器学习算法识别和验证涉及类风湿性关节炎的新型生物标记物:硅内和体内研究
IF 4.6 3区 医学
Mediators of Inflammation Pub Date : 2024-02-14 DOI: 10.1155/2024/3188216
Fucun Liu, Juelan Ye, Shouli Wang, Yang Li, Yuhang Yang, Jianru Xiao, Aimin Jiang, Xuhua Lu, Yunli Zhu
{"title":"Identification and Verification of Novel Biomarkers Involving Rheumatoid Arthritis with Multimachine Learning Algorithms: An In Silicon and In Vivo Study","authors":"Fucun Liu, Juelan Ye, Shouli Wang, Yang Li, Yuhang Yang, Jianru Xiao, Aimin Jiang, Xuhua Lu, Yunli Zhu","doi":"10.1155/2024/3188216","DOIUrl":"https://doi.org/10.1155/2024/3188216","url":null,"abstract":"<i>Background</i>. Rheumatoid arthritis (RA) remains one of the most prevalent chronic joint diseases. However, due to the heterogeneity among RA patients, there are still no robust diagnostic and therapeutic biomarkers for the diagnosis and treatment of RA. <i>Methods</i>. We retrieved RA-related and pan-cancer information datasets from the Gene Expression Omnibus and The Cancer Genome Atlas databases, respectively. Six gene expression profiles and corresponding clinical information of GSE12021, GSE29746, GSE55235, GSE55457, GSE77298, and GSE89408 were adopted to perform differential expression gene analysis, enrichment, and immune component difference analyses of RA. Four machine learning algorithms, including LASSO, RF, XGBoost, and SVM, were used to identify RA-related biomarkers. Unsupervised cluster analysis was also used to decipher the heterogeneity of RA. A four-signature-based nomogram was constructed and verified to specifically diagnose RA and osteoarthritis (OA) from normal tissues. Consequently, RA-HFLS cell was utilized to investigate the biological role of <i>CRTAM</i> in RA. In addition, comparisons of diagnostic efficacy and biological roles among <i>CRTAM</i> and other classic biomarkers of RA were also performed. <i>Results</i>. Immune and stromal components were highly enriched in RA. Chemokine- and Th cell-related signatures were significantly activated in RA tissues. Four promising and novel biomarkers, including <i>CRTAM</i>, <i>PTTG1IP</i>, <i>ITGB2</i>, and <i>MMP13</i>, were identified and verified, which could be treated as novel treatment and diagnostic targets for RA. Nomograms based on the four signatures might aid in distinguishing and diagnosing RA, which reached a satisfactory performance in both training (AUC = 0.894) and testing (AUC = 0.843) cohorts. Two distinct subtypes of RA patients were identified, which further verified that these four signatures might be involved in the immune infiltration process. Furthermore, knockdown of <i>CRTAM</i> could significantly suppress the proliferation and invasion ability of RA cell line and thus could be treated as a novel therapeutic target. CRTAM owned a great diagnostic performance for RA than previous biomarkers including <i>MMP3</i>, <i>S100A8</i>, <i>S100A9</i>, <i>IL6</i>, <i>COMP</i>, <i>LAG3</i>, and <i>ENTPD1</i>. Mechanically, CRTAM could also be involved in the progression through immune dysfunction, fatty acid metabolism, and genomic instability across several cancer subtypes. <i>Conclusion</i>. <i>CRTAM</i>, <i>PTTG1IP</i>, <i>ITGB2</i>, and <i>MMP13</i> were highly expressed in RA tissues and might function as pivotal diagnostic and treatment targets by deteriorating the immune dysfunction state. In addition, <i>CRTAM</i> might fuel cancer progression through immune signals, especially among RA patients.","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"139 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139767858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Qingfei Tongluo Mixture Attenuates Bleomycin-Induced Pulmonary Inflammation and Fibrosis through mTOR-Dependent Autophagy in Rats. 清瘟通络散通过mTOR依赖性自噬减轻博莱霉素诱导的大鼠肺部炎症和纤维化
IF 4.6 3区 医学
Mediators of Inflammation Pub Date : 2024-02-08 eCollection Date: 2024-01-01 DOI: 10.1155/2024/5573353
Shuyu Ge, Zhenghong Guo, Ting Xiao, Pingping Sun, Bo Yang, Yin Ying
{"title":"Qingfei Tongluo Mixture Attenuates Bleomycin-Induced Pulmonary Inflammation and Fibrosis through mTOR-Dependent Autophagy in Rats.","authors":"Shuyu Ge, Zhenghong Guo, Ting Xiao, Pingping Sun, Bo Yang, Yin Ying","doi":"10.1155/2024/5573353","DOIUrl":"10.1155/2024/5573353","url":null,"abstract":"<p><p>As an interstitial fibrosis disease characterized by diffuse alveolitis and structural alveolar disorders, idiopathic pulmonary fibrosis (IPF) has high lethality but lacks limited therapeutic drugs. A hospital preparation used for the treatment of viral pneumonia, Qingfei Tongluo mixture (QFTL), is rumored to have protective effects against inflammatory and respiratory disease. This study aims to confirm whether it has a therapeutic effect on bleomycin-induced IPF in rats and to elucidate its mechanism of action. Male SD rats were randomly divided into the following groups: control, model, CQ + QFTL (84 mg/kg chloroquine (CQ) + 3.64 g/kg QFTL), QFTL-L, M, H (3.64, 7.28, and 14.56 g/kg, respectively) and pirfenidone (PFD 420 mg/kg). After induction modeling and drug intervention, blood samples and lung tissue were collected for further detection. Body weight and lung coefficient were examined, combined with hematoxylin and eosin (H&E) and Masson staining to observe lung tissue lesions. The enzyme-linked immunosorbent assay (ELISA) and the hydroxyproline (HYP) assay kit were used to detect changes in proinflammatory factors (transforming growth factor-<i>β</i> (TGF-<i>β</i>), tumor necrosis factor-<i>α</i> (TNF-<i>α</i>), and interleukin-1<i>β</i> (IL-1<i>β</i>)) and HYP. Immunohistochemistry and Western blotting were performed to observe changes in proteins related to pulmonary fibrosis (<i>α</i>-smooth muscle actin (<i>α</i>-SMA) and matrix metalloproteinase 12 (MMP12)) and autophagy (P62 and mechanistic target of rapamycin (mTOR)). Treatment with QFTL significantly improved the adverse effects of bleomycin on body weight, lung coefficient, and pathological changes. Then, QFTL reduced bleomycin-induced increases in proinflammatory mediators and HYP. The expression changes of pulmonary fibrosis and autophagy marker proteins are attenuated by QFTL. Furthermore, the autophagy inhibitor CQ significantly reversed the downward trend in HYP levels and <i>α</i>-SMA protein expression, which QFTL improved in BLM-induced pulmonary fibrosis rats. In conclusion, QFTL could effectively attenuate bleomycin-induced inflammation and pulmonary fibrosis through mTOR-dependent autophagy in rats. Therefore, QFTL has the potential to be an alternative treatment for IPF in clinical practice.</p>","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"2024 ","pages":"5573353"},"PeriodicalIF":4.6,"publicationDate":"2024-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10869187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139741335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cryptotanshinone Inhibits Bladder Cancer Cell Malignant Progression in a Lipopolysaccharide-Induced Inflammatory Microenvironment through NLRP3 Inhibition 隐丹参酮通过抑制 NLRP3 抑制脂多糖诱导的炎症微环境中膀胱癌细胞的恶性进展
IF 4.6 3区 医学
Mediators of Inflammation Pub Date : 2024-01-30 DOI: 10.1155/2024/8828367
Chenye Tang, Xiao Guo, Yu Li, Yongxiang Yi, Zhiling Tang, Qihui Zhang, Bairu Luo, Kean Chen, Ke Liang, Gang Li
{"title":"Cryptotanshinone Inhibits Bladder Cancer Cell Malignant Progression in a Lipopolysaccharide-Induced Inflammatory Microenvironment through NLRP3 Inhibition","authors":"Chenye Tang, Xiao Guo, Yu Li, Yongxiang Yi, Zhiling Tang, Qihui Zhang, Bairu Luo, Kean Chen, Ke Liang, Gang Li","doi":"10.1155/2024/8828367","DOIUrl":"https://doi.org/10.1155/2024/8828367","url":null,"abstract":"<i>Background</i>. Bladder cancer (BC) is one of the most common malignancies of the urogenital system. This study assessed the nucleotide-binding oligomerization domain and leucine-rich repeat and pyrin domain-containing protein 3 (NLRP3) in BC as well as the effects of cryptotanshinone on changes in BC malignant behaviors and NLRP3 expression under a lipopolysaccharide (LPS)-induced inflammatory microenvironment. <i>Methods</i>. BC tissue specimens from 62 patients were collected for immunohistochemical detection of NLRP3 protein. BC and normal urothelial cell lines were cultured for the detection of NLRP3 mRNA and protein. Then, BC cells were pretreated with LPS to mimic the inflammatory tumor microenvironment. Next, these cells were incubated with a low or high dose of cryptotanshinone to assess its effects on tumor cell malignant behaviors as well as transfected with NLRP3 cDNA to confirm the role of NLRP3 in BC cells <i>in vitro</i>. <i>Results</i>. High NLRP3 expression was associated with larger tumor diameters (&gt;2 cm), muscle invasion, and metastasis. The levels of NLRP3 mRNA and protein were greater in BC cells than in normal urothelial cells. LPS pretreatment significantly promoted NLRP3 and inflammatory cytokine expression in BC cells, and induced cell viability, migration, and invasion. However, cryptotanshinone was able to reduce the LPS-induced increase of NLRP3 and inflammatory cytokine expression as well as the BC cell malignant progression. NLRP3 overexpression using NLRP3 cDNA further promoted BC cell malignant progression after LPS stimulation and reversed cryptotanshinone-reduced LPS-induced BC cell malignant behaviors. <i>Conclusion</i>. NLRP3 might possess oncogenic activity in BC, and the antitumor activity of cryptotanshinone in BC <i>in vitro</i> might be related to its inhibition of NLRP3 expression.","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"38 5 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139586992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Photoprotective Effects of Epigallocatechin Gallate on Ultraviolet-Induced Zebrafish and Human Skin Fibroblasts Cells 表没食子儿茶素没食子酸酯对紫外线诱导的斑马鱼和人类皮肤成纤维细胞的光保护作用
IF 4.6 3区 医学
Mediators of Inflammation Pub Date : 2024-01-24 DOI: 10.1155/2024/7887678
Jie Zhang, Yahui Xu, Xiyu Ruan, Ting Zhang, Minghui Zi, Qiao Zhang
{"title":"Photoprotective Effects of Epigallocatechin Gallate on Ultraviolet-Induced Zebrafish and Human Skin Fibroblasts Cells","authors":"Jie Zhang, Yahui Xu, Xiyu Ruan, Ting Zhang, Minghui Zi, Qiao Zhang","doi":"10.1155/2024/7887678","DOIUrl":"https://doi.org/10.1155/2024/7887678","url":null,"abstract":"<i>Background</i>. The long-term exposure to ultraviolet radiation (UVR) raises oxidative stress and chronic inflammation levels, which in turn has a series of deleterious effects on skin health, such as sunburn, photoaging, and skin cancer. Hence, our study was determined to investigate the effects and mechanisms of epigallocatechin gallate (EGCG) in zebrafish and human skin fibroblasts (HSF) cells to alleviate ultraviolet-induced photoaging. <i>Methods</i>. The 4 days postfertilization (dpf) zebrafish larvae and HSF cells were treated with 10 J/cm<sup>2</sup> UVA + 30 mJ/cm<sup>2</sup> UVB, or 25, or 50 <i>μ</i>M EGCG for 72 hr. The indicators involving in oxidative stress, inflammatory, and photoaging were measured by the kits, ELISA Kits and western blot methods. <i>Results</i>. EGCGs protect against UVR-induced skin damage in zebrafish and HSF cells. EGCG markedly decreased the reactive oxygen species (ROS), malondialdehyde, 8-OHdG levels, increased superoxide dismutase (SOD) activity, and significantly inhibited inflammatory factors levels including tumor necrosis factor-<i>α</i> (TNF-<i>α</i>), interleukin-1<i>α</i> (IL-1<i>α</i>), interleukin-6 (IL-6) in zebrafish, and HSF cells irradiated with UVR. We found that EGCG could reduce UVR-induced p38 mitogen-activated protein kinase (p38 MAPK) phosphorylation and effectively inhibited the activity of the transcriptional factor nuclear factor-<i>κ</i>B (NF-<i>κ</i>B), thereby reducing the protein-1 (AP-1), TNF-<i>α</i>, IL-1<i>α</i>, IL-6, and matrix metalloproteinase-1 (MMP-1) expressions, which are critical mediators of skin aging cascade causing the photoaging. <i>Conclusion</i>. These results validate that EGCG for protection of photoaging in zebrafish and HSF cells induced by UVR, which is closely related to the regulation of p38 MAPK/NF-<i>κ</i>B, AP-1 signaling pathway which relieve oxidative stress, inflammation, and collagen degradation.","PeriodicalId":18371,"journal":{"name":"Mediators of Inflammation","volume":"212 1","pages":""},"PeriodicalIF":4.6,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139561339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信